Priyanka Saha

3440 Olentangy River Road, Apt 6E Columbus, OH 43202

sahap@mail.gvsu.edu·1-616-920-9390

CAREER OBJECTIVE To obtain a position that will enable me to use my strong organizational skills, educational background, and ability to work well with people. I am skilled in conducting complex research projects with team members. I have efficiency in isolating and maintaining cultures of microorganisms, sterilization process, cell and tissue culture. I have experience of handling mammalian cell culture and maintain them for a long time. My internship helped me to understand the cellular and molecular events that underlie the pathogenesis of Parkinson’s disease. My molecular biology proficiency includes PCR, DNA and RNA isolation and purification, restriction analysis of DNA, primer design and sequence analysis. I have worked on animal model. I would like to join some R&D where I will be able to use my skills to profit of the organization or company.

EDUCATION

Professional Science Master (PSM) – Biotechnology 2013-2015

Grand Valley State University, Grand Rapids, Michigan

Master of Science in Botany - First Class 2006-2008

University of Calcutta, Scottish Church College, Kolkata, India

Bachelor of Science in Botany - First Class 2003-2006

University of Calcutta, Lady Brabourne College, Kolkata, India

Bachelor of Education (B.Ed.) - First Class 2010-2011

State Government University, Kolkata, India

INTERNSHIP

Intern at Michigan State University (Van- Andel Institute), Grand Rapids, MI, under Dr. Katrina L.

Paumier, Translational Science & Molecular Medicine (TSMM) Department (from August 2014- present).

1) Inhibiting the production of alpha- synuclein (α-syn) will prevent the spread and toxicity

induced by pathological α-syn.

Aim: To observe the role of different antisense oligonucleotides (ASO’s) to inhibit the α-syn

aggregation and dopamine cell death.

2) Antidepressants are neuroprotective in Parkinson’s disease.

Aim: Whether different antidepressants (especially amitriptyline) reduce the spread and

accumulation of a-syn, thus reducing dopamine toxicity.

PROJECT WORK

Chemical and Herbal Induction of Resistance in Soybean [Glycine max (L) MERRILL] Against

Anthracnose.

Dr. Shampa Bhattacharyya and Dr. Rajyasri Ghosh from Scottish Church College, India.

PURPOSE OF THE PROJECT-

To induce systemic resistance in susceptible cultivars of soybean against Anthracnose

disease by different kinds of defense activators (chemical and herbals).

To analyze protein profiles and to detect defense related proteins (DRPs) in

leaves of soybean plants treated with different defense activators.

To test the growth response of pathogen (Colletotrichum sp.) to plant defense

activators in vitro.

LABORATORY TOOLS AND TECHNIQUES Plants:

Plant tissue staining and Histology

Preparation of slides

Tissue mounting

Plant quadrat

Mushroom cultivation

Cell culture maintaining ( fungal and bacterial cell culture)

Using of pH meter

Quantification of Carbohydrate, Protein, Lipid

Sterile techniques

Microscopy- light, confocal, DIC & fluorescent ( moderate knowledge)

Spectrophotometer (for protein assays)

Spectroscopy measurement and analysis of nucleic acids

Agarose and SDS Gel Electrophoresis

Centrifuge

Primer design, PCR, RT-PCR

Restriction digestion

Plasmid/DNA/RNA purification & isolation (Plasmid miniprepes & alkaline lysis plasmid minipreps)

Sequence analysis (basic)

Plant and animal tissue culture

Basic 3D cell culture

Animals: Staining free-floating tissue (Immunohistochemistry).

Stereology and cell counting.

Cell culturing (MDA-MB-231and HEK-293 cell lines, Nigral/ Striatal cell).

Use of microtome-cutting brain section.

How to handle rats

How to run behavioral tests (Cylinder, sucrose, inky paws, forced swim test, novel object recognition test, anosmia test, priming test etc.)

Cut rat brains on a sliding microtome

Staining free-floating tissue (Immunohistochemistry)

Mount tissue on slides

Cover slipping slides

Implant a sub cutaneous pump in a rat

Prep for surgery

Perfusion surgery

Preparation of primary nerve cell culture

Making solutions

EXPERIENCE Graduate Assistant

Course- Scientific Communication & Perspectives in CMB 2014- April 2015

Grand Valley State University

Assistant Teacher- Biology

Thakurnagar girls’ high school (Higher Secondary), India 2011-2012

Assistant Teacher- Biology

Bandipur Ideal Academy for Girls, India 2012-2013