principles of biochemistry
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PRINCIPLES OF BIOCHEMISTRY. Chapter 28 Regulation of Gene Expression. 28.1 Principles of Gene Regulation 28.2 Regulation of Gene Expression in Bacteria 28.3 Regulation of Gene Expression in Eukaryotes. p.1115. - PowerPoint PPT PresentationTRANSCRIPT
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PRINCIPLES OF BIOCHEMISTRY
Chapter 28
Regulation of Gene Expression
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28.1 Principles of Gene Regulation
28.2 Regulation of Gene Expression
in Bacteria28.3 Regulation of Gene
Expression in Eukaryotes
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The cellular concentration of a protein is determined by a delicate balance of at least seven processes, each having several potential points of regulation:
1. Synthesis of the primary RNA transcript (transcription)
2. Posttranscriptional modification of mRNA
3. Messenger RNA degradation
4. Protein synthesis (translation)
5. Posttranslational modification of proteins
6. Protein targeting and transport
7. Protein degradation
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FIGURE 28-1
FIGURE 28–1 Seven processes that affect the steady-state concentration of a protein.
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Genes expressed at a more or less constant level in virtually every cell of a species or organism, are often referred to as housekeeping genes. Unvarying expression of a gene is called constitutive gene expression.
Gene products that increase in concentration under particular molecular circumstances are referred to as inducible; the process of increasing their expression is induction.
Gene products that decrease in concentration in response to a molecular signal are referred to as repressible, and the process is called repression.
28.1 Principles of Gene Regulation
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RNA Polymerase Binds to DNA at Promoters
Most E. coli promoters have a sequence close to a consensus (Fig. 28–2).
Transcription Initiation Is Regulated by Proteins That Bind to or near Promoters
At least three types of proteins regulate transcription initiation by RNA polymerase: specificity factors alter the specificity of RNA polymerase for a given promoter or set of promoters; repressors impede access of RNA polymerase to the promoter; and activators enhance the RNA polymerase–promoter interaction.
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FIGURE 28-2
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FIGURE 28–2 Consensus sequence for many E. coli promoters.
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FIGURE 28-3
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FIGURE 28–3 Consensus sequence for promoters that regulate expression of the E. coli heat shock genes.
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Repressors bind to specific sites on the DNA. In bacterial cells, such binding sites, called operators.
Regulation by means of a repressor protein that blocks transcription is referred to as negative regulation.
Activators provide a molecular counterpoint to repressors; they bind to DNA and enhance the activity of RNA polymerase at a promoter; this is positive regulation.
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FIGURE 28-4(a)
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FIGURE 28–4 Common patterns of regulation of transcription initiation.
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FIGURE 28-4(b)
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FIGURE 28-4(c)
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FIGURE 28-4(d)
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Many Bacterial Genes Are Clustered and Regulated in Operons
The gene cluster and promoter, plus additional sequences that function together in regulation, are called an operon (Fig. 28–5).
The genes were those for β-galactosidase, which cleaves lactose to galactose and glucose, and galactoside permease (lactose permease), which transports lactose into the cell (Fig. 28–6).
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FIGURE 28-5
FIGURE 28–5 Representative bacterial operon.
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FIGURE 28-6
FIGURE 28–6 Lactose metabolism in E. coli.
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The lac Operon Is Subject to Negative Regulation
The lactose (lac) operon (Fig. 28–7a) includes the genes for β-galactosidase (Z), galactoside permease (Y), and thiogalactoside transacetylase (A).
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FIGURE 28-7(a)
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FIGURE 28–7 The lac operon.
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FIGURE 28-7(b)
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FIGURE 28-7(c)
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FIGURE 28-7(d)
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Regulatory Proteins Have Discrete DNA-Binding Domains
Most of the chemical groups that differ among the four bases and thus permit discrimination between base pairs are hydrogen-bond donor and acceptor groups exposed in the major groove of DNA (Fig. 28–8).
The two hydrogen bonds that can form between Gln or Asn and the N6 and N-7 positions of adenine cannot form with any other base. And an Arg residue can form two hydrogen bonds with N-7 and O6 of guanine (Fig. 28–9).
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FIGURE 28-8
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FIGURE 28–8 Groups in DNA available for protein binding.
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FIGURE 28–9
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FIGURE 28–9 Two examples of specific amino acid residue–base pairinteractions that have been observed in DNA-protein binding.
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FIGURE 28-10
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FIGURE 28–10 Relationship between the lac operator sequence O1 and the lac promoter.
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Several DNA-binding motifs have been described, but here we focus on two that play prominent roles in the binding of DNA by regulatory proteins: the helix-turn- helix and the zinc finger.
Helix-Turn-Helix The helix-turn-helix motif comprises about 20 amino acids in two short α-helical segments, each seven to nine amino acid residues long, separated by a β turn (Fig. 28–11).
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Zinc Finger Several hydrophobic side chains in the core of the structure also lend stability. Figure 28–12 shows the interaction between DNA and three zinc fingers of a single polypeptide from the mouse regulatory protein Zif268.
Homeodomain This domain of 60 amino acids—called the homeodomain, because it was discovered in homeotic genes (genes that regulate the development of body patterns).
The DNA sequence that encodes this domain is known as the homeobox.
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FIGURE 28–11(a)
p.1123FIGURE 28–11 Helix-turn-helix.
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FIGURE 28–11(b)
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FIGURE 28–11(c)
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FIGURE 28–11(d)
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FIGURE 28–12
p.1123FIGURE 28–12 Zinc fingers.
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FIGURE 28–13
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FIGURE 28–13 Homeodomain.
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Regulatory Proteins Also Have Protein-Protein Interaction Domains
Two important examples are the leucine zipper and the basic helix-loop-helix.
Leucine Zipper This motif is an amphipathic helix with a series of hydrophobic amino acid residues concentrated on one side (Fig. 28–14).
Basic Helix-Loop-Helix The helix-loop-helix motifs of two polypeptides interact to form dimers (Fig. 28–15).
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FIGURE 28–14(a)
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FIGURE 28–14 Leucine zippers.
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FIGURE 28–14(b)
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FIGURE 28-15
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FIGURE 28–15 Helix-loop-helix.
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28.2 Regulation of Gene Expressionin BacteriaThe lac Operon Undergoes Positive Regulation
A regulatory mechanism known as catabolite repression restricts expression of the genes required for catabolism of lactose, arabinose, and other sugars in the presence of glucose, even when these secondary sugars are also present. The effect of glucose is mediated by cAMP, as a coactivator, and an activator protein known as cAMP receptor protein, or CRP.
The effect of glucose on CRP is mediated by the cAMP interaction (Fig. 28–18).
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FIGURE 28–16
FIGURE 28–16 CRP homodimer. p.1126
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FIGURE 28–17
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FIGURE 28–17 Activation of transcription of the lac operon by CRP.
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FIGURE 28–18
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FIGURE 28–18 Combined effects of glucose and lactose on expression of the lac operon.
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Many Genes for Amino Acid Biosynthetic Enzymes Are Regulated by Transcription Attenuation
The E. coli tryptophan (trp) operon (Fig. 28–19) includes five genes for the enzymes required to convert chorismate to tryptophan.
The Trp repressor is a homodimer, each subunit containing 107 amino acid residues (Fig. 28–20).
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FIGURE 28–19
p.1128FIGURE 28–19 The trp operon.
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FIGURE 28–20
p.1128FIGURE 28–20 Trp repressor.
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Once repression is lifted and transcription begins, the rate of transcription is fine-tuned by a second regulatory process, called transcription attenuation.
The trp operon attenuation mechanism uses signals encoded in four sequences within a 162 nucleotide leader region at the 5' end of the mRNA, preceding the initiation codon of the first gene (Fig. 28–21a). Within the leader lies a region known as the attenuator, made up of sequences 3 and 4.
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FIGURE 28–21(a)
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FIGURE 28–21 Transcriptional attenuation in the trp operon.
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FIGURE 28–21(b) Part 1
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FIGURE 28–21(b) Part 2
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FIGURE 28–21(c)
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Induction of the SOS Response Requires Destruction of Repressor Proteins
The LexA repressor (Mr 22,700) inhibits transcription of all the SOS genes (Fig. 28–22).
Synthesis of Ribosomal Proteins Is Coordinated with rRNA Synthesis
One r-protein encoded by each operon also functions as a translational repressor, which binds to the mRNA transcribed from that operon and blocks translation of all the genes the messenger encodes (Fig. 28–23).
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FIGURE 28–22
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FIGURE 28–22 SOS response in E. coli.
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FIGURE 28–23
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FIGURE 28–23 Translational feedback in some ribosomal protein operons.
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The regulation coordinated with amino acid concentrations is known as the stringent response (Fig. 28–24).
Amino acid starvation leads to the binding of uncharged tRNAs to the ribosomal A site; this triggers a sequence of an enzyme called stringent factor (RelA protein) to the ribosome.
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FIGURE 28–24
p.1132FIGURE 28–24 Stringent response in E. coli.
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The Function of Some mRNAs Is Regulated by Small RNAs in Cis or in Trans
A well-characterized example of RNA regulation in trans is seen in the regulation of the mRNA of the gene rpoS (RNA polymerase sigma factor), which encodes σS, one of the seven E. coli sigma factors.
The σS mRNA is present at low levels under most conditions but is not translated, because a large hairpin structure upstream of the coding region inhibits ribosome binding (Fig. 28–25).
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FIGURE 28-25(a)
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FIGURE 28–25 Regulation of bacterial mRNA function in trans by sRNAs.
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FIGURE 28-25(b)
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Regulation in cis involves a class of RNA structures known as riboswitches.
Binding of an mRNA’s riboswitch to its appropriate ligand results in a conformational change in the mRNA, and transcription is inhibited by stabilization of a premature transcription termination structure, or translation is inhibited (in cis) by occlusion of the ribosome-binding site (Fig. 28–26).
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FIGURE 28-26
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FIGURE 28–26 Regulation of bacterial mRNA function in cis byriboswitches.
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28.3 Regulation of Gene Expression in EukaryotesTranscriptionally Active Chromatin Is Structurally Distinct from Inactive Chromatin
Heterochromatin, is transcriptionally inactive. Heterochromatin is generally associated with particular chromosome structures—the centromeres, for example. The remaining, less condensed chromatin is called euchromatin.
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Chromatin Is Remodeled by Acetylation and Nucleosomal Displacement/Repositioning
The transcription-associated structural changes in chromatin are generated by a process called chromatin remodeling.
The acetylation and methylation of histones figure prominently in the processes that activate chromatin for transcription.
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These methylations facilitate the binding of histone acetyltransferases (HATs), enzymes that acetylate particular Lys residues.
When transcription of a gene is no longer required, the extent of acetylation of nucleosomes in that vicinity is reduced by the activity of histone deacetylases (HDACs).
DNA-Binding Activators and Coactivators Facilitate Assembly of the General Transcription Factors
These additional regulatory sequences are usually called enhancers in higher eukaryotes and upstream activator sequences (UASs) in yeast.
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Successful binding of active RNA polymerase II holoenzyme at one of its promoters usually requires the action of other proteins (Fig. 28–29), of four types:
(1) transcription activators, which bind to enhancers or UASs and facilitate transcription;
(2) chromatin modification and remodeling proteins, described above;
(3) coactivators; and
(4) basal transcription factors, required at every Pol II promoter.
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FIGURE 28-29(a)
p.1139FIGURE 28–29 Eukaryotic promoters and regulatory proteins.
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FIGURE 28-29(b)
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Transcription Activators These high mobility group (HMG) proteins (refers to their electrophoretic mobility in polyacrylamide gels) play an important structural role in chromatin remodeling and transcriptional activation.
Coactivator Protein Complexes The principal eukaryotic coactivator consists of 20 to 30 or more polypeptides in a protein complex called mediator.
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TATA-Binding Protein The first component to bind in the assembly of a preinitiation complex (PIC) at the TATA box of a typical Pol II promoter is the TATAbinding protein (TBP).
Choreography of Transcriptional Activation We can now begin to piece together the sequence of transcriptional activation events at a typical Pol II promoter (Fig. 28–30).
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FIGURE 28–30 Part 1
FIGURE 28–30 The components of transcriptional activation.
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FIGURE 28–30 Part 2
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The Genes of Galactose Metabolism in Yeast Are Subject to Both Positive and Negative Regulation
Some of the general principles described above can be illustrated by one well-studied eukaryotic regulatory circuit (Fig. 28–31).
Other protein complexes also have a role in activating transcription of the GAL genes. These include the SAGA complex for histone acetylation, the SWI/SNF complex for nucleosome remodeling, and the mediator complex.
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FIGURE 28-31
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FIGURE 28–31 Regulation of transcription of GAL genes in yeast.
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FIGURE 28-32
FIGURE 28–32 Protein complexes involved in transcriptional activationof a group of related eukaryotic genes.
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Transcription Activators Have a Modular Structure
Experiments that substitute a variety of different peptide sequences for the acidic activation domain of Gal4p suggest that the acidic nature of this domain is critical to its function, although its precise amino acid sequence can vary considerably.
A wide variety of other activator proteins also have these glutamine-rich domains.
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FIGURE 28-33(a)
p.1142FIGURE 28–33 Transcription activators.
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FIGURE 28-33(b)
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Eukaryotic Gene Expression Can Be Regulated by Intercellular and Intracellular Signals
The hormone-receptor complex acts by binding to highly specific DNA sequences called hormone response elements (HREs), thereby altering gene expression.
The hormone receptors have a highly conserved DNA-binding domain with two zinc fingers (Fig. 28–34).
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FIGURE 28-34
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FIGURE 28–34 Typical steroid hormone receptors.
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Regulation Can Result from Phosphorylation of Nuclear Transcription Factors
The β-adrenergic pathway that leads to elevated levels of cytosolic cAMP, which acts as a second messenger in eukaryotes as well as in bacteria, also affects the transcription of a set of genes, each of which is located near a specific DNA sequence called a cAMP response element (CRE).
The catalytic subunit of protein kinase A, released when cAMP levels rise, enters the nucleus and phosphorylates a nuclear protein, the CRE-binding protein (CREB).
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Many Eukaryotic mRNAs Are Subject to Translational Repression
Some proteins bind directly to mRNA and act as translational repressors (Fig. 28-35).
A protein kinase called HCR (hemin-controlled repressor) is activated, catalyzing the phosphorylation of eIF2.
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FIGURE 28-35
p.1145FIGURE 28–35 Translational regulation of eukaryotic mRNA.
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Posttranscriptional Gene Silencing Is Mediated by RNA Interference
In higher eukaryotes, including nematodes, fruit flies, plants, and mammals, a class of small RNAs called micro-RNAs (miRNAs).
Many miRNAs are present only transiently during development, and these are sometimes referred to as small temporal RNAs (stRNAs).
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If an investigator introduces into an organism a duplex RNA molecule corresponding in sequence to virtually any mRNA, Dicer cleaves the duplex into short segments, called small interfering RNAs (siRNAs).
These bind to the mRNA and silence it (Fig. 28–36b). The process is known as RNA interference (RNAi).
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FIGURE 28-36
p.1146FIGURE 28–36 Gene silencing by RNA interference.
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RNA-Mediated Regulation of Gene Expression Takes Many Forms in Eukaryotes
All RNAs that do not encode proteins, including rRNAs and tRNAs, come under the general designation of ncRNAs (noncoding RNAs).
Development Is Controlled by Cascades of Regulatory Proteins
The life cycle of the fruit fly includes complete metamorphosis during its progression from an embryo to an adult (Fig. 28–37).
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FIGURE 28-37
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FIGURE 28–37 Life cycle of the fruit fly Drosophila melanogaster.
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Among the most important characteristics of the embryo are its polarity and its metamerism.
The Drosophila egg, along with 15 nurse cells, is surrounded by a layer of follicle cells (Fig. 28–38).
Maternal genes are expressed in the unfertilized egg, and the resulting maternal mRNAs remain dormant until fertilization.
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Segmentation genes, transcribed after fertilization, direct the formation of the proper number of body segments. At least three subclasses of segmentation genes act at successive stages: gap genes, and pair-rule genes together with segment polarity genes.
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FIGURE 28-38 Part 1
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FIGURE 28–38 Early development in Drosophila.
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FIGURE 28-38 Part 2
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FIGURE 28-39(a)
FIGURE 28–39 Distribution of a maternal gene product in a Drosophila egg.
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FIGURE 28-39(b)
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Segmentation Genes Gap genes, pair-rule genes, and segment polarity genes, three subclasses of segmentation genes in Drosophila, are activated at successive stages of embryonic development.
One well-characterized segmentation gene is fushi tarazu (ftz), of the pair-rule subclass.
The mRNAs and proteins derived from the normal ftz gene accumulate in a striking pattern of seven stripes that encircle the posterior two-thirds of the embryo (Fig. 28–41).
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FIGURE 28-41
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FIGURE 28–41 Distribution of the fushi tarazu (ftz) gene product inearly Drosophila embryos.
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Homeotic Genes A set of 8 to 11 homeotic genes directs the formation of particular structures at specific locations in the body plan. These genes are now more commonly referred to as Hox genes.
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FIGURE 28-42(a)
FIGURE 28–42 The Hox gene clusters and their effects on development.
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FIGURE 28-42(b)
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FIGURE 28-43
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FIGURE 28–43 Effects of mutations in Hox genes in Drosophila.