prince henry’s institute monash medical centre 246 clayton road, clayton, victoria contacts: dr....
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Prince Henry’s InstituteMonash Medical Centre246 Clayton Road, Clayton, Victoria
Contacts:Dr. Morag Young: [email protected]. Peter Fuller: [email protected]
Steroid Receptor Biology
Mineralocorticoid receptors (MR) and Heart DiseaseMineralocorticoid receptors (MR) and Heart Disease
Heart disease is a leading cause of death in Australia (40%) MR are a key players in this pathology…but how?!
Dr Morag YoungDr Morag Young
Uninephrectomised mouse/rat treated
with aldosterone/DOC plus 0.9% saline
Cardiac FibrosisHypertension Cardiac Hypertrophy
Oxidative Stress
NADPH oxidase
InflammationCOX-2
Osteopontin
Macrophages
8 days
8 weeks
We have 3 tissue selective MR knockout mouse strains that we are using to determine the specific role of the MR in heart
Mineralocorticoid receptors (MR) and Heart DiseaseMineralocorticoid receptors (MR) and Heart DiseaseDr Morag YoungDr Morag Young
Fibrillar collagen
Fibroblast
VSMC
Endothelial cell
Cardiac myocyte
Artery
Macrophage MR-null mice:
No effect on DOC-induced monocyte/macrophage recruitment
Reduced basal gene expression
Cardiac myocyte MR-null mice:
No effect on DOC-induced monocyte/macrophage recruitment
No effect on inflammatory gene expression
Endothelial cell MR-null mice:
Reduced DOC-induced macrophage recruitment at 8 days
MR
MRMR
Granulosa Cell Tumours (GCT)Granulosa Cell Tumours (GCT)Granulosa Cell Tumours (GCT)Granulosa Cell Tumours (GCT)
• Comprise 5% of malignant ovarian tumours• Molecular analysis of tumour bank and cell lines•Tissue selective knockout of IKK signalling in ovaries
Our focus has been on:• profiles of gene expression: candidates & microarray • signalling pathways – constitutive activation• expression of ER
•coregulatory molecules• ERβ-induced genes
• mutation detection•Implications for normal granulosa cells
Professor Peter FullerProfessor Peter Fuller