primary immunodeficiency preeda sangacharoenkit m.d. division of allergy and immunology department...
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Primary Immunodeficiency
Preeda Sangacharoenkit M.D.Division of Allergy and Immunology
Department of PediatricsBangkok Metropolitan Administrative
Medical Colleague
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Cooper et al. American Academy of Family Physicians November 2003.
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Cooper et al. American Academy of Family Physicians November 2003.
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Rosenzweig and Holland. J Allergy Ciln Immunol April 2004.
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Evaluation of T-cell function
• Quantitative evaluation
: T-cell subpopulation
• Qualitative evaluation
: Delayed-type hypersensitivity (DTH) skin testing
Fleisher and Oliveira. J Allergy Clin Immunol August 2004.
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Evaluation of phagocytic function
• The nitroblue tetrazolium test (NBT)
• Fluorescent cytometry using the dihydrorhodamine-123 (DHR) probe
Segal and Holland. Pediatric Clinics of North America December 2000.
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Roos et al. Microbes and Infection 5 (2003)
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Panel D shows the results of the nitroblue tetrazolium test in normal neutrophils: phagocytosis results in dark-blue staining of the cytoplasm. Panel E shows the results of the nitroblue tetrazolium test in neutrophils from a patient with chronic granulomatous disease: there is no phagocytosis and thus no dark-blue cytoplasmic staining.
Lekstrom-Himes and Gallin. NEJM December 2000.
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Jirapongsananuruk et al. J Allergy Clin Immunol February 2003.