presented at sevpac 2007 – permission granted for use on sevpac website only 06-1536 shelley j...
TRANSCRIPT
![Page 1: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/1.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
06-1536
Shelley J Newman DVM, DVSc, DACVP
M. Souza, C. Greenacre, G. Daniels
![Page 2: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/2.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Signalment
Female,10 year old
Yellow-naped
Amazon parrot
![Page 3: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/3.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
History
10 day history of anemia,
lymphocytic leukocytosis
and lethargy
![Page 4: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/4.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Physical exam
No abnormalities
noted initially
![Page 5: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/5.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Serial Chemistry Results
• Hypoproteinemia
• Hypoalbuminemia
• Hypophosphatemia
• Hypocholesterolemia
• Monoclonal gammopathy
(Beta region)
![Page 6: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/6.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Diagnostic Work up
Imaging
• Contrast radiography
• Contrast CT
• PETscan
![Page 7: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/7.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 8: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/8.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 9: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/9.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 10: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/10.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 11: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/11.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 12: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/12.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Histopathology
• Expansion and infiltration
• Round cell population
• Abundant cytoplasm
• Round vesicular nuclei
• Prominent multiple nucleoli
• Rare mitoses
• Anisokaryosis and anisocytosis
• Little intra-epithelial involvement
![Page 13: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/13.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Special Stains
• ZN and Fites acid fast stains were negative
• Considered cell size and plasmacytoid differentiation as indication of plasma cell tumor or lymphosarcoma
• Recommended immunohistochemistry
![Page 14: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/14.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Monoclonal CD3
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 15: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/15.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Monoclonal CD3
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 16: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/16.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
IMHC Results
• CD79 did not produce staining in the avian splenic control or the intestinal infiltrate
• BLA.36 did not produce staining in the avian splenic control
• CD3 polyclonal and CD3 monoclonal stained splenic control tissue and the neoplastic cells
![Page 17: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/17.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Diffuse Intestinal T cell Lymphosarcoma
![Page 18: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/18.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Outcome
• Owner elected euthanasia after seven weeks of chemotherapy
![Page 19: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/19.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 20: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/20.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website onlyPresented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
![Page 21: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/21.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Discussion
• LSA is rare in birds but typically presents as cutaneous or peri-orbital lesions
• Not thought to be virally induced in psittacines
![Page 22: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/22.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Discussion
• No previous reports of diffuse T cell intestinal lymphosarcoma in psittacines
• Prognosis is poor despite chemotherapy as with the T cell immunophenotype in mammals.
• Unique findings in this case included gammopathy and lymphocytic leukosis
![Page 23: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/23.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Monoclonal Gammopathy (Beta)
• Might have more strongly supported B cell origin
• Been reported twice before with psittacine T cell lymphosarcoma
• Unlikely to result from unidentified infectious etiology
![Page 24: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/24.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
References
• Ramos-Vara JA, Smith EJ, Watson GL. Lymphosarcoma with plasmacytoid differentiation in a Scarlet macaw. Avian Dis 1997, 41: 499-504.
• de Wit M, Schoemaker NJ, Kik MJL, Westerhof I. Hypercalcemia in two amazon parrots with malignant lymphoma. Avian Dis 2003, 47:223-228.
![Page 25: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/25.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Acknowledgements
• Dale Godson – Prairie Diagnostics
• Histopathology Laboratory - UT
• Ladonna Mrkonjich
• Dr. Donnell
• Dr. Roslyn Casimir
![Page 26: Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only 06-1536 Shelley J Newman DVM, DVSc, DACVP M. Souza, C. Greenacre, G. Daniels](https://reader034.vdocuments.us/reader034/viewer/2022052618/551bfe635503469e4f8b4aba/html5/thumbnails/26.jpg)
Presented at SEVPAC 2007 – Permission granted for use on SEVPAC website only
Questions??