prednisone and azathioprine compared with prednisone and ...€¦ · prednisone (both survive with...
TRANSCRIPT
546 Blood, Vol 72, No 2 (August), 1988: pp 546-554
Prednisone and Azathioprine Compared With Prednisone and Placebo forTreatment of Chronic Graft-v-Host Disease: Prognostic Influence of
Prolonged Thrombocytopenia After Allogeneic Marrow Transplantation
By K.M. Sullivan, R.P. Witherspoon, R. Storb, P. Weiden, N. Flournoy, S. Dahlberg, H.J. Deeg, J.E. Sanders,
K.C. Doney, F.R. Appelbaum, R. McGuffin, G.B. McDonald, J. Meyers, M.M. Schubert, J. Gauvreau,
H.M. Shulman, G.E. Sale, C. Anasetti, T.P. Loughran, S. Strom, J. Nims, and E.D. Thomas
We conducted a randomized. double-blind comparison of
prednisone and placebo (group I) v prednisone and azathio-
prine (1 .5 mg/kg/day) (group II) as early treatment of
extensive chronic graft-v-host disease (GVHD). Patients
with platelet counts <100.0O0/�sL were placed into ther-
apy with prednisone alone (group III). All three groups
received identical doses of prednisone (1 mg/kg every
other day) and one double-strength trimethoprim-sulfame-
thoxazole (TMP-SMX) tablet twice daily. Between January
1 980 and December 1 983. 1 79 previously untreated
patients were enrolled and 1 64 were evaluable. Patients
randomized to group I (n = 63) and group II (n - 63) were
well matched for prognostic factors; those placed into
group III (n - 38) had more frequent acute GVHD and
progressive onset of chronic GVHD. Median duration of
therapy was 2 years. Complications included diabetes
(5%). aseptic necrosis (5%) and infection. For groups I, II.
and Ill, the respective incidence of infection was dissemi-
nated varicella. 1 1 %. 24%. 34%; bacteremia, 6%. 11%.
C HRONIC graft-v-host disease (GVHD) is a major late
complication in 25% to 45% of survivors of allogeneic
marrow transplantation.’3 Less than 20% of patients with
untreated extensive chronic GVHD survive with Karnofsky
performance scores �7O%.� Therapy with antithymocyte
globulin or corticosteroids given late in the course of disease
results in little benefit.2 In a pilot study of combined immu-
nosuppression with cytotoxic agents and corticosteroids, I 6
of 21 patients survive free of disability.2 However, compari-
son of therapy with azathioprine and prednisone to our
previous experience with prednisone alone was complicated
by the fact that patients given the combination were treated
34%; and interstitial pneumonia. 5%. 1 4%, 1 8%. Recurrent
malignancy was the most frequent cause of death and did
not differ significantly across the groups. Nonrelapse mor-
tality, however. did differ: 21 % in group I. 40% in group II,
and5s%ingrouplll(l vlI.P= .003;l vlll.P=- .001). Forty
patients in group I, 30 in group II, and 10 in group Ill survive
with a minimum follow-up of 3.8 years. Karnofsky perfor-
mance scores for 68 survivors are 90% to 1 00%, scores for
seven survivors are 70% to 89% and scores for five
survivors are <70%. Actuarial survival at 5 years after
transplant is 61 % in group I, 47% in group II, and 26% in
group III (I v II. P = .03; I v III. P = .0001 ). Treatment with
prednisone alone results in fewer infections and better
survival than prednisone and azathioprine in standard-risk
chronic GVHD. Treatment with prednisone alone is less
effective in high-risk patients with thrombocytopenia. and
other strategies are required.
a 1988 by Grune & Stratton. Inc.
in more recent times when earlier diagnosis and therapy,
better supportive care and closer follow-up were used.
Because the benefits and toxicities of long-term cytotoxic
therapy after marrow grafting are unclear, we studied in a
randomized, double-blind trial the use of prednisone and
placebo compared with prednisone and azathioprine in early
treatment of chronic GVHD. Patients with poor marrow
function as a contraindication to azathioprine (platelet
counts < 100,000/zL) were placed into treatment with pred-
nisone alone. We report the results of this controlled clinical
trial, which has a minimum follow-up of 3.8 years after
transplant.
MATERIALS AND METHODS
From the Fred Hutchinson Cancer Research Center and the
University of Washington School ofMedicine, Seattle.
Submitted August 12. 1987; accepted March 29. 1988.
Supported by US Public Health Service Grants No. CA 18221.
CA 18029. CA 09515, CA 15704 from the National Cancer Insti-tute. and HL 36444from the National Institute ofHeart. Lung and
Blood, Department ofHealth and Human Services.
Presented in part at the 29th Annual Meeting of the AmericanSociety ofHematology. Washington. DC. December 5-8. 1987.
Dr Thomas is a recipient of Research Career Award Al 02425from the National Institute of Allergy and Infectious Diseases,
DHHS. Dr. Loughran is a Fellow of the Leukemia Society ofAmerica.
Address reprint requests to Keith M. Sullivan. MD. Fred Hut-
chinson Cancer Research Center, Department ofMedicine. DivisionofOncology, I 124 Columbia St. Seattle, WA 98104.
The publication costs of:his article were defrayed in part by page
charge payment. This article must therefore be hereby marked
“advertisement” in accordance with 18 U.S.C. section 1 734 solely to
indicate this fact.
© 1988 by Grune & Stratton. Inc.
0006-4971/88/7202-0019$3.00/0
From January 1980 to December 1983, 179 patients with exten-
sive (multiorgan) chronic GVHD entered the study. Seventyenrolled patients were randomized to receive prednisone and placebo(group I), and 7 1 were randomized to receive prednisone and
azathioprine (group II). An additional 38 patients with thrombocy-topenia (group III) were placed into treatment with prednisonealone. When an interim analysis showed an increased mortality,4group III was closed and treatment was modified to include alternat-
ing cyclosporine and prednisone. Results of that subsequent studywere presented in another report.5
Fifteen (1 1%) of 141 enrolled patients randomized to groups I and
II had violations of the double-blind treatment protocol and were notevaluable for response. Five patients refused treatment after enroll-ment because they considered that therapy was not required. Fiverefused the study drug and two took open-label azathioprine becausethey or their physicians wished to choose the specific drug regimen.The remaining three patients discontinued treatment before com-pleting the 9-month schedule. The results in these I 5 inevaluable
patients were as follows. In group I, three refused therapy (two died
and one survived with 40% performance); one stopped treatmentafter 3 months and died; one refused study drug, took prednisone and
azathioprine, and died; and two refused study drug and took
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prednisone (both survive with 100% performance). In group II, two Patients with aplastic anemia were prepared for transplant withrefused therapy (both died); three refused study drug (one died, one cyclophosphamide, 50 mg/kg on each of four successive days.’
survived with 50% performance, and one survived with 100% perfor- Patients with hematologic malignancies received high-dose cyclo-mance); one complied poorly with treatment and died; one refused phosphamide or other chemotherapy regimens followed by totalstudy drug, took prednisone and azathioprine, and died; and one body irradiation (TBI) given as a single lO-Gy dose or as a
stopped treatment after 6 months and survived with 100% perfor- fractionated I 2- to I 7.5-Gy dose.7’8 All but 14 patients receivedmance. marrow from HLA-identical siblings; those 14 received marrow
Demographic data of 164 evaluable patients are listed in Table 1. from HLA-nonidentical donors.’ Three HLA-identical recipients
Table 1 . Patient Characteristics at Study Entry
Randomized Placed
Prednisone AlonePrednisone + Placebo Prednisone + Azathioprine
Characteristic (Group I) (Group II) (Group III)
No. of evaluable patients 63 63 38
0
35
2
0
PREDNISONE ± AZATHIOPRINE IN CHRONIC GVHD 547
Abbreviations: ALL, acute lymphoblastic leukemia; ANL, acute nonlymphoblastic leukemia; CML, chronic myelogenous leukemia; GVHD, graft-v-host
disease; MTX, methotrexate.
Diagnosis
Aplastic anemia
Refractory ANL/ALL
Relapse ANL/ALL
2nd+ Remission ANL/ALL
1st Remission ANL/ALL
Accelerated o�’ blast crisis CML
Chronic phase CML
Lymphoma/other
No. (%) of patients
9
2/0
6/6
1/10
13/3
4
7
2/0
7
4/0
8/6
1/3
12/3
8
7
2/2
2
0/0
1/3
2/8
1 1/1
4
4
2/0
<10 yr
10-30 yr
>30 yr
Median (range) age in years
Sex (M/F)
HLA-nonidentical donor (%)
Bone marrow T-ceII depletion
Acute GVHD prophylaxis
None
ShortMTX(11 d)
Standard MTX (102 d)
Cyclosporine (180 d)
MTX/cyclosporine ( 1 1 / 1 80 d)
Grade of Acute GVHD
0
II
III
IV
Total grade ll-lV (%)
Type of onset of chronic GVHD (%)
De novo
Quiescent
Progressive
Presentation of chronic GVHD (%)
Subclinical always
Subclinical -‘ clinical
Clinical always
No. (%) Entering treatment
�4 mo after transplant
5- 1 2 mo after transplant
> 1 2 mo after transplant
Median (range) values at entry
Time to treatment (months after transplant)
Karnofsky score (%)
Serum bilirubin (mg/dI)
WBC count (x 1034iL)
Platelet count (x 103/s1)
8(13)
42 (66)
13 (21)
23 (2-43)
44/19
4 (6)
0
5
46
9
3
21
13
19
10
0
29 (46)
21 (33)
31 (49)
11 (17)
6(10)
18 (28)
39 (62)
35 (56)
21 (33)
7(11)
3 (2-38)
70(60-95)
0.9 (0.2-14.0)
5.4 (1.6-19.0)
170(100-442)
5 (8)
44(70)
14 (22)
24 (1-48)
47/16
4 (6)
0
6
49
5
3
24
12
11
16
0
27 (43)
24 (38)
27 (43)
12 (19)
8 (13)
17 (27)
38 (60)
34 (54)
26 (41)
3 (5)
4 (2-33)
70 (30- 100)
0.7 (0.2-39.0)
5.4 (2.2-20.0)
168(100-600)
6 (16)
22 (58)
10(26)
23 (3-47)
28/10
6 (16)
8
6
9
14
24 (63)
8 (21)
17 (45)
13 (34)
5 (13)
9 (24)
24 (63)
33 (87)
5 (13)
0
3 (3-8)
70(30-90)
1.0 (0. 1-14.0)
4.3 (1.5-14.8)
39 (6-80)
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548 SULLIVAN ET AL
were given marrow purged of T lymphocytes.’#{176} All others received
unmodified allogeneic marrow. The prophylaxis, grading, and treat-ment ofacute GVHD were reported �
Chronic GVHD had a progressive onset if it followed as a direct
extension of acute GVHD. Quiescent chronic GVHD developed
after resolution of acute GVHD, whereas de novo chronic GVHDwas not preceded by acute GVHD. Diagnosis was established upon
review of clinical, laboratory, and histologic data by previouslypublished criteria.2” Subclinical chronic GVHD was defined ashistologic evidence ofchronic GVHD on both the blind skin and oralbiopsies without signs or symptoms of clinical disease. Clinicalchronic GVHD was defined as both histologic and clinical evidence
of chronic GVHD. At study entry, all patients were in hematologicremission with donor marrow engraftment and had not receivedprior treatment for chronic GVHD. Previous GVHD prophylaxis
was discontinued upon study entry.Protocols and consent forms were approved by the Institutional
Review Board of the Fred Hutchinson Cancer Research Center. All
three groups received prednisone given in oral divided doses forweeks I and 2 and thereafter as a single oral morning dose (Table 2).
Patients in groups I and II received study drug in addition toprednisone. Study drug was assigned by random permutations of a
set of numbers known only to the protocol registrar and was suppliedas unmarked 50-mg scored tablets of either placebo or azathioprine.
Study drug was given as a single evening dose of 1.5 mg/kg/day.Patients in all three treatment groups received prophylactic
TMP-SMX. Adults received one double-strength (160 mg TMP)tablet twice daily, and children received 75 mg/m2 TMP twice daily.
Patients with life-threatening TMP-SMX allergies received prophy-lactic penicillin or cephalexin. Patients with less severe allergic
histories were rechallenged with TMP-SMX. Patients receivedsupportive care with artificial tear replacements, sun-blocking
creams, and oral caloric and protein supplements as required.
After 9 months of treatment, patients were reevaluated in Seattlewith physical examination, assessment of Karnofsky performancescore, laboratory studies (blood urea nitrogen, serum creatinine,
complete blood count, and liver function tests), pulmonary functiontests, Schirmer’s test and biomicroscopy, nutritional status, and
routine skin and oral mucosa biopsies and other biopsies as� The drug code was not broken and response totreatment was determined in a blinded manner. The followingcriteria were used to judge treatment response. Progressive disease
after 2 months of treatment or stable disease with persistingKarnofsky scores <50% after 9 months of treatment was consideredno response. The drug code was broken at study failure. Clinically
inactive chronic GVHD, but biopsies showing continued GVHD
activity with no new organ involvement after 9 months of therapy
was considered partial response. Clinically inactive disease andbiopsies showing no GVHD activity after 9 months of therapy was
Table 2. Alterna te-Day Predn isone Regimen
Prednisone
WeekofTherapy
(mg/kg/�aIly)
DayA DayS
1 1.0 1.0
2 1.0 1.0
3 2.0 0.5
4 2.0 0.25
5 2.0 0.12
6 2.0 0
7 1.5 0
8 1.25 0
9-36 1.0 0
considered complete response. When treatment was stopped after a
complete response but clinical and histologic disease activityreturned, the patient was considered to have a flare of chronic
GVHD. Therapy was reinstituted for another 9 months. For patientswith a complete response, therapy was discontinued. For patients
with a partial response, treatment continued. If chronic GVHDsymptoms were clinically active and disabling after 18 months oftreatment, patients were declared a failure.
Results were analyzed as of June 1, 1987. Survival rates wereestimated by the Kaplan-Meier method, and comparison statistics(two-sided significance levels) were calculated using the log-rankand stratified log-rank tests.’9’�#{176}
RESULTS
Entry data. As shown in Table 1 , patients randomized to
groups I and II were well matched for treatment and
prognostic factors. Twenty-four (38%) patients in group I, 25
(40%) in group II, and 14 (37%) in group III started
treatment while chronic GVHD was still subclinical. Forty-
four (70%) of these 63 patients with subclinical disease at
diagnosis developed clinical chronic GVHD during treat-
ment. Only in 19 (12%) of the 164 evaluable patients did
chronic GVHD remain subclinical throughout therapy.
Patients in group III did not appear to differ from random-
ized patients in diagnosis, age, acute GVHD prophylaxis,
pretreatment Karnofsky scores or bilirubin values, or time to
treatment. By definition, all had platelet counts <100,000/
sL. The leukocyte counts were slightly lower in group III
patients, but only two patients had leukocytes <2,000/zL at
entry. Median pretreatment bone marrow cellularity was
80% of normal in group I, 80% of normal in group II, and
50% of normal in group III.
Response. Nine months after starting therapy, 47 (29%)
of 164 evaluable patients had died or relapsed, 26 (16%) had
failed therapy, and 41 (25%) had a partial response and 50
(30%) had a complete response to treatment (Table 3).
Complete responses occurred in 21 (33%) patients ofgroup I,
23 (37%) ofgroup II, and six (16%) ofgroup III.
Long-term follow-up. Among I I 7 patients surviving in
hematologic remission who completed 9 months of therapy
(Table 3), 50 (93%) of 54 patients in group I, 40 (89%) of 45
patients in group II, and I 5 (83%) of 1 8 patients in group III
returned one or more times to Seattle for reevaluation.
Toxicity and infection. Thirty (18%) of the 164 evalu-
able patients developed one or more of the toxicities listed in
Table 3. Twenty-nine (46%) patients in group I, 39 (62%) in
group II, and 28 (74%) in group III developed one or more
infections listed in Table 3. Disseminated varicella zoster,
bacteremia, and interstitial pneumonia were more frequent
in patients randomized to receive azathioprine as compared
with placebo recipients. Patients in group III had more
frequent infections than did patients in group I who received
identical treatment.
Among 28 episodes of bacteremia, eight were owing to
pneumococcus (five occurring when patients were off TMP-
SMX), six Staphylococcus, six Pseudomonas, five Hemo-
phi/us influenzae, and three other gram-negative organisms.
Twenty episodes of interstitial pneumonia (six Pneumocystis
pneumoniae, five varicella, four idiopathic, three cytomega-
lovirus (CMV), one Legionella, and one herpes simplex)
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PREDNISONE ± AZATHIOPRINE IN CHRONIC GVHD 549
Table 3. Results of Treatment
Randomized Placed
Prednisone + Placebo Prednisone + Azathioprine Prednisone Alone
Result (Group I) (Group II) (Group Ill)
No. of evaluable patients 63 63 38
Response (%) after 9-mo therapy
Complete response 21 (33) 23 (37) 6 (16)
Partial response 18 (29) 17 (27) 6 (16)
Failed treatment 1 5 (24) 5 (8) 6 (16)
Died or Relapsed before 9 mo 9 (14) 18 (29) 20 (52)
Median (range) Karnofsky score after 9-mo therapy (%)
Complete responders 100 (90-100) 100 (80-100) 100 (90-100)
Partial responders 95 (80-100) 90 (80-100) 90 (80- 100)
Failed treatment 70 (30-95) 30 (30-90) 70 (50-90)
Toxicity of therapy
Diabetes mellitus 3 5 1
Aseptic necrosis 4 3 1
Neutrophils < 1 ,000/ML 0 4 2
Platelets < 1 00.00O/�L 1 3 -
Gastrointestinal hemorrhage 1 2 2
Severe osteoporosis 1 0 1
Psychosis 1 0 0
Episodes of infection (no. of patients, percentage of patients)
Varicella zoster (total) 18 (18, 29%) 25 (25. 40%) 17 (15, 39%)
Localized 1 1 (1 1. 17%) 10(10, 16%) 2 (2. 5%)
Disseminated 7(7. 11%) 15(15,24%) 15(13,34%)
Bacteremia 4 (4, 6%) 7 (7. 1 1%) 17 (13, 34%)
Interstitial pneumonia 3 (3. 5%) 9 (9. 14%) 8 (7, 18%)
Noninterstitial pneumonia 13 (10, 16%) 14 (10, 16%) 17 (13, 34%)
were observed. All six episodes of Pneumocystis pneumoniae
developed in 31 patients either not given or discontinuing
TMP-SMX. Forty-four episodes of noninterstitial pneumo-
nia developed, 21 owing to unknown organisms and the
remainder owing to Pneumococcus ( 1 1 ), Hemophilus
influenzae (four), fungus (four), gram-negative organisms
(two) and staphylococcus (two).
Drug dosage. Prednisone was given in the first 9 months
in full dose schedule to 141 (86%) patients; the remaining
patients had some dose modifications owing to the toxicities
listed in Table 3. Permanent reduction in prednisone dose
was observed in eight (5%) patients: three to 75% to 99%,
three to 50% to 74%, and two to 25% to 40% protocol dose.
Azathioprine was given in reduced dose in four (6%)
patients: two to 75% to 99% and two to 50% to 74% protocol
dose. Prophylactic TMP-SMX was not given or was discon-
tinued owing to side effects in nine (14%) patients in group I,
nine ( 14%) in group II, and I 3 (34%) in group III. Twelve of
these 3 1 patients received no additional antibiotics. The
remaining 19 received cephalexin (nine), penicillin (eight),
or ampicillin (two).
Clinical manifestations. Sites of disease noted in 145
patients with clinical chronic GVHD were dermal (79%),
hepatic (73%), oral (72%), ophthalmic (47%), intestinal
(16%), myofascial (11%), pulmonary (1 1%), esophageal
(6%), and serosal (2%). Intestinal chronic GVHD was more
frequent in patients in group III. Laboratory abnormalities
included rheumatoid factor reactivity (16%), hypogamma-
globulinemia (IgG < 650 mg/dL, 15%) and autoantibody
production (8%). Scleroderma with joint contractures devel-
oped in 1 1 (8%) of 145 evaluable patients with clinical
chronic GVHD.
Mortality. Twenty-three (37%) patients in group I, 33
(52%) in group II, and 28 (74%) in group III died (Table 4).
Recurrent malignancy was the most common cause of death.
Kaplan-Meier estimates of recurrent malignancy were 26%
in group I, 33% in group II, and 39% in group III (I v II,
P = .95; I v III, P = .23). Infection was the most common
cause of nonrelapse mortality. The remaining deaths were
owing to progressive GVHD without infection, hemorrhage,
or organ failure. One patient with a long pretransplant
history of paranoia refused psychiatric therapy and commit-
ted suicide.
Figure 1 shows the probability of death from nonrelapse
causes. By actuarial estimate, 21% of patients in group I,
40% in group II, and 58% in group III died of nonmalignant
causes (P = .0001). Among randomized patients, nonrelapse
mortality was significantly increased in azathioprine recip-
ients (P = .003). When group I was compared to group III,
the increase in nonrelapse mortality in patients with throm-
bocytopenia was significant (P = .0001).
Prognostic factors. Mortality did not appear related to
gender, acute GVHD prophylaxis, or time to study entry.
Table 5 lists factors in relation to mortality from all causes.
Age >20 years (P = .02), prior grade II through IV acute
GVHD (P = .03), the progressive onset of chronic GVHD
(P = .0001), and failure to respond to 9 months of treatment
(P = .04) were associated with increased mortality in log-
rank analyses stratified by treatment group.
Actuarial mortality in 19 patients in whom chronic
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550 SULLIVAN ET AL
Table 4. Causes of Death
Randomized Placed
Prednisone + Placebo Prednisone + Azathioprine Prednisone AloneCause (n-63) (n-63) (n-38)
Bacterial infection 2 1 0 6
Interstitial pneumonia
Varicella zoster 1 1 2
Cytomegalovirus 0 0 2
Idiopathic 0 2 0
Pneumocystis 0 1 0
Other Infection 1 1 0
Fungal infection 1 3 3
Progressive GVHD without infection 1 1 1
Respiratory failure without IP 1 1 1
Heart failure 0 1 1
Varicella zoster without IP 1 1 0
Hemorrhage 0 1 1
Myasthenia gravis 0 0 1
Suicide 1 0 0
Recurrent malignancy 14 10 10
Total 23 33 28
Abbreviations: GVHD, graft-versus-host disease; IP, interstitial (nonbacterial, nonfungal) pneumonia.
100 GVHD remained subclinical throughout therapy was 58% as
80 p<00001 compared with 50% mortality in 46 patients with subclinicalTh,’ornbocylopenio, p/OCed: , clinical chronic GVHD and 52% mortality in 99 patients
0 -1�� P’ednisone(n-38) with clinical chronic GVHD at diagnosis. Patients with/0 40 � Prednisone #Azothioprine(n�63) sustained subclinical chronic GVHD had higher mortality
Randomized: owing to an increased rate of relapse of leukemia. Figure 22: � ‘P,edn,sone#P/oceboj’n:63) shows the probabilityofrelapse in 146 patients with hemato-
0 1 2 3 4 5 6 7 8 9 10 11 logic malignancies in relation to the course of chronicYears after Transplant GVHD. Three-way statistical analysis showed that relapse of
malignancy was significantly increased in patients in whomFig 1 . Kaplan-Meier product limit estimates of the probability chronic GVHD remained subclinical throughout observation
of death from nonrelapse (ie. transplant-related) causes. (P = .0003).
Table 5. Mortality Risk Factors
No. of Dead/No. of Evaluable Patients
Groupl Groupll Grouplll Total(%)
Factor (n-63) (n-63) (n-38) (n- 164)
Patient age
�20yr 8/31 6/17 11/17 25/65 (38)
>20 yr 15/32 27/46 17/21 59/99 (60)
Grade acute GVHD
0 6/21 10/24 4/8 20/53 (38)
I 5/13 7/12 4/6 16/31 (52)
II 6/19 6/11 8/9 20/39 (51)
Ill 6/10 10/16 11/14 27/40(68)
IV 0/0 0/0 1/1 1/1 (100)
Type of chronic GVHD onset
Denovo 6/21 10/24 4/8 20/53 (38)
Quiescent 12/31 14/27 12/17 38/75 (51)
Progressive 5/11 9/12 12/13 26/36 (72)
Presentation of chronic GVHD
Subclinical always 3/6 4/8 4/5 1 1/1 9 (58)
Subclinical -. clinical 7/ 1 8 6/ 1 7 7/9 20/44 (45)
Clinical always 13/39 23/38 17/24 53/101 (52)
Response after 9-mo therapy
Complete response 2/21 7/23 1/6 10/50 (20)
Partialresponse 5/18 4/17 4/6 13/41 (31)
Failed treatment 7/1 5 4/5 3/6 14/26 (54)
Died or relapsed before 9 mo 9 1 8 20 47
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600J‘0
40
20
C
100
80
60
40
20
PREDNISONE ± AZATHIOPRINE IN CHRONIC GVHD 551
100
80 p(O.0003
� CIWOyS (n�/9)
(C//fl/CO/ 0/wayS (n�85)
Years after Transplant
Fig 2. Kaplan-Meier product limit estimates of the probabilityof relapse of malignancy in 1 9 patients with subclinical -‘ subclini-cal chronic GVHD. 42 patients with subclinical -‘ clinical chronic
GVHD. and 85 patients with clinical chronic GVHD. Eighteen of the164 evaluable patients (16 with clinical and 2 with subclinical
clinical chronic GVHD) receiving transplants for nonmalignantdisorders were excluded from this analysis.
Status ofsurvivors. Forty (63%) patients in group I, 30
(48%) patients in group II, and 10 (26%) patients in group
III survive (Table 6). Minimum follow-up is 45 months after
transplant. Nine (14%) patients with complete response in
group I, six (10%) in group II, and two (5%) in group III had
flares of chronic GVHD a median of 2 months (range one to
1 1 months) after stopping therapy and were retreated.
Primary sites of flare included skin (ten), liver (four), mouth
(one), eye (one), and muscle (one). Currently, 65 (81%) of
the survivors continue off treatment and are free of chronic
GVHD. The Karnofsky performance scores of the 80 surviv-
ing patients are shown in Table 6.
Figure 3 shows the actuarial survival. Survival estimates
were 61% in group I, 47% in group II, and 26% in group III
(P = .001 ). When randomized patients in groups I and II
were compared, survival was reduced in azathioprine recip-
ients (P = .03). When groups I and III were compared,
survival was impaired in patients with thrombocytopenia
(P= .0001).
DISCUSSION
The well-being of the marrow graft recipient may depend
on events developing long after the patient leaves the trans-
plant center. Collaboration between the primary care physi-
RondomizedPrednisone # Placebo (n�63)
Randomized
Prednisone #Aza/fliopnne (n:63)
rh(ombocy/openio, placedp<o.oo1 Prednisone (n�38)
C0 1 2 3 4 5 6 7 8 9 10 11
Years after Transplant
Fig 3. Kaplan-Meier product limit estimates of the probabilityof survival among 63 evaluable patients in group I (predni-sone + placebo). 63 evaluable patients in group II (predni-
sone + azathioprine), and 38 patients in group Ill (prednisone). Ticmarks represent 40 group I, 30 group II, and 10 group Ill patientssurviving as of June 1 #{149}1 987. Minimum follow-up is 3.8 years after
transplant.
cian and investigators at the center assumes increasing
importance as the success and use of marrow transplantation
increases.2’ This collaboration is vital in conducting long-
term, double-blind clinical trials such as we describe. We
found that with appropriate education and responsiveness,
physician and patient compliance was enthusiastic and only
15 (8%) of 179 patients enrolled in the study were not
evaluable. Moreover, although 80% of our transplant recip-
ients reside outside the Pacific Northwest, I 05 (90%) of I I 7
patients completing 9 months of protocol therapy returned to
Seattle for long-term follow-up evaluation.
With early treatment, runting, scleroderma, and joint
contractures are now uncommon developments. Indeed, most
patients have normal Karnofsky scores, and only five (6%) of
80 survivors function at <70% performance. With improved
disability-free survival, new manifestations of chronic
GVHD, such as obliterative bronchiolitis2225 and myasthenia
gravis,26’27 may be observed. Nevertheless, with minimum
follow-up of 3.8 years and with 65 (81%) of 80 survivors
currently off all treatment, the present study appears suffi-
ciently mature to detect most causes of disability.28
The double-blind, placebo-controlled design allowed
assessment of the role of azathioprine in early treatment of
chronic GVHD. Prior clinical2 and experimental29 studies
Table 6. Status of Survivi ng Patients
5tatus
Randomized Placed
Prednisone Alone(n - 38)
Prednisone + Placebo
(n - 63)Prednisone + Azathioprine
(n - 63)
No. of surviving patients
Median (range) follow-up
Months after transplant
Months after study entry
Treatment characteristics
Median (range) duration of therapy (mo)
No. of patients still on therapy (%)
No. of patients stopping therapy
No. of patients still off therapy
Median (range) duration off therapy (mo)
Current Karnofsky score
100%-90%
89%-70%
<70%
40
68 (48-125)
63 (42-88)
24 (9-72)
8 (20)
35
32
48 (3-78)
34
4
2 (5%)
30
72 (51-95)
64 (44-90)
22 (9-90)
6 (20)
28
24
46 (2 1 -74)
26
3
1 (3%)
10
66 (45-89)
6 1 (42-85)
24 (9-57)
1 (10)
10
9
33 (2-64)
8
0
2 (20%)
For personal use only.on October 22, 2017. by guest www.bloodjournal.orgFrom
552 SULLIVAN ET AL
suggested that azathioprine has activity against GVHD. The
current trial is the first randomized study of chronic GVHD,
and with its design the effect of immunodeficiency owing to
uncontrolled GVHD and immunodeficiency owing to cyto-
toxic therapy could be dissociated and analyzed. Surprising-
ly, with early treatment the control ofGVHD was equivalent
in recipients of prednisone alone and prednisone plus aza-
thioprine. However, viral and bacterial infections were more
frequent with combination treatment. The nonrelapse mor-
tality was significantly increased in standard-risk patients
receiving additional azathioprine (21% in group I v 40% in
group II). Interstitial pneumonia appeared to be increased30
but hepatic abnormalities3’ and significant myelosuppression
were not more common in azathioprine recipients.
The study design also allowed assessment of the contribu-
tion of cytotoxic therapy in preventing recurrent malignancy
after transplant. The relapse rates did not differ significantly
in placebo and azathioprine recipients. In previous studies,
the contribution of chronic GVHD to an antileukemic effect
had been less clear since patients with more severe chronic
GVHD were usually treated with azathioprine.32’33 The pres-
ent study fails to show an antileukemic effect of azathioprine
treatment.
Although some experimental studies suggest that graft-
v-leukemia (GVL) reactivity can be obtained without
augmenting GVHD,34’35 other studies question this dissocia-
tion.36 The current clinical trial does not support the sugges-
tion that GVHD can be separated from the GVL effect.
Patients with chronic GVHD remaining subclinical through-
out treatment had a 55% probability of relapse as compared
with 28% and 25% rates of relapse, respectively, in patients
with clinical or subclinical �- clinical chronic GVHD
(P .0003). This increased recurrence of leukemia nullified
any survival advantage that might be gained in treating
subclinical disease.
Infection remains a distressingly frequent complication of
chronic GVHD. Patients with thrombocytopenia have a poor
response to therapy and an increased number of infections
even without cytotoxic treatment. This dynamic relation of
chronic GVHD to opportunistic infection and recovery of
immune function is a complex, interrelated, and time-
dependent process.37 Numerous reports demonstrate multi-
plc B and T cell defects in patients with chronic GVHD.3”2
Patients with chronic GVHD often develop bacterial and
. interstitial pneumonias and bacteremia.43’” Infection with
encapsulated organisms such as Pneumococcus and Hemo-
phi/us influenzae are especially frequent.45 Use of prophy-
lactic TMP-SMX reduces the incidence of late interstitial
pneumonia in patients with chronic GVHD. In a previous
report of patients with chronic GVHD, we found a 28%
actuarial incidence of late interstitial pneumonia in patients
treated with prednisone without TMP-SMX v I 5% in
untreated patients (ie, no immunosuppression and no antibi-
otics) v 8% in patients treated with prednisone and TMP-
SMX (P = .001).” Because patients with chronic GVHD
demonstrate impaired immunoglobulin regulation and a
pattern of infection resembling that associated with the
primary immunodeficiencies, long-term administration of
intravenous (IV) immune globulin may be another method to
prevent infection.�’ Controlled studies are currently in prog-
ress to test this hypothesis. Finally, prompt recognition and
treatment of varicella zoster can also lower mortality.
Because of the seriousness of varicella in these patients,
prompt treatment with IV acyclovir appears to be fully
warranted.47
Prognostic factors in this study included patient age, acute
GVHD, progressive onset of chronic GVHD, failure to
respond to 9 months of therapy, and thrombocytopenia. The
reason for increased mortality in patients with thrombocyto-
penia is poorly understood” Pretreatment thrombocytope-
nia was associated with impaired marrow function, as cvi-
denced by a median megakaryocyte cellularity of 50% of
normal (compared to 80% median megakaryocyte cellularity
in patients with normal platelet counts). Viral-induced or
cell-mediated49 suppression of megakaryocytopoiesis could
account for persisting thrombocytopenia. Recent in vitro
studies suggest immune-mediated defects in hematopoietic
progenitor cell function in patients with chronic GVHD.5#{176}
The use of cyclosporine in this setting of thrombocytopenia
appears to be of value and is the subject of another report.5
We conclude that in patients with chronic GVHD with
normal marrow function, prednisone led to fewer infections
and better survival than therapy with prednisone and aza-
thioprine. Early treatment abated the disabling natural
history of clinical extensive chronic GVHD. Patients with
subclinical GVHD did not benefit from therapy due to an
increased rate of recurrent leukemia. Persisting thrombocy-
topenia identified a group of patients with poor response to
prednisone and frequent, often fatal, infection. Improved
supportive care and treatment are needed for high-risk
chronic GVHD patients who have poor marrow function or
who fail initial therapy.
ACKNOWLEDGMENT
We are indebted to referring physicians for management of the
patients as well as inpatient and outpatient physicians, nurses, andsupport staff of the Fred Hutchinson Cancer Research Center andSwedish Hospital Medical Center. We appreciate the support ofJack Pelkey and Burroughs Wellcome, Research Triangle Park,
NC, for their support and supply of the study drug. We especiallythank Deborah Gayle and Marianne Hansen for superb assistance inlong-term follow-up.
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1988 72: 546-554
Doney and FR AppelbaumKM Sullivan, RP Witherspoon, R Storb, P Weiden, N Flournoy, S Dahlberg, HJ Deeg, JE Sanders, KC prolonged thrombocytopenia after allogeneic marrow transplantationtreatment of chronic graft-v-host disease: prognostic influence of Prednisone and azathioprine compared with prednisone and placebo for
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