Pre Breeding WorkshopPre-Breeding Workshop,activities & intro to discussionsdiscussions

ECPGR Barley WGECPGR Barley WG10-12.05.2011marja.jalli@mtt.fi

Pre-breedingPre breeding

Definition:

all activities designed to identify desirable characteristics/genes from unadapted PGRcharacteristics/genes from unadapted PGR and to transfer them to an intermediate product that breeders can manipulate

Pre-Breeding for disease resistance in FI

GENETIC RESOURCES

CHARACTERIZATION OF HE PATHOGEN

GENE BANK GENE BANK

SCREENING FOR RESISTANCE SOURCES

CHARACTERIZATIONCHARACTERIZATIONOF THE RESISTANCE

DEVELOPMENTOF GENETIC MARKERS

RESEARCH RESEARCH

BACKCROSSINGPROGRAMME

PYRAMIDING

PLANT BREEDINGPLANT BREEDING

RESISTANCE GENES

VARIETY

CLEAN RAW MATERIALFOR FOOD PROCESSING

INDUSTRY

ENVIRONMENTAL &

FARMER

INDUSTRY

Elcio Guimaraes:

• population growth, urbanization, loss in diversity etc. motivate for pre-breeding

• a process referred to as prebreeding or genetic enhancement is often necessary to• a process referred to as prebreeding or genetic enhancement is often necessary to produce material that can be easily used by breeders

• challenges:

1) Activities related to PGR: high cost and long term return 2) Lack of characterization and evaluation data 3) Knowledge on the genetic diversity, local knowledge and diversity important –3) Knowledge on the genetic diversity, local knowledge and diversity important information from them, knowledge of the inter species relationship 4) Encourage the development of public-private cooperation5) Prebreeding needs a strong breeding program to operate under

http://www.nordgen.org/ngdoc/plants/PPP_workshop_2011/2_SettingTheScene_ElcioGuimares.pdfp

Minimizing the energy use in cropping s stemscropping systems

for ex fertilizers, pesticidesPGR &

PRE-BREEDINGPGR &

PRE BREEDING

CLIMATECHANGE

Local foodproduction

Energy & waterfor production

PRE BREEDING PRE-BREEDINGCHANGE

FOOD FOREVERYONE

A t bl i

Plants adaptedto environment

Plants withgood nutritional

value

CLIMATECHANGE

CLIMATECHANGE

Acceptable pricesfor agricultural

products

Good fertilityof soil

PGR &PRE-BREEDING PGR &

CLIMATE

Soil & nature saving cropping systems

PGR &PRE-BREEDINGCLIMATE

CHANGE

Soil & nature saving cropping systems(for ex no-tillage, organic)

ProposalProposalTo establish a cooperative project under ECPGR cereal network on pre-breedingunder ECPGR cereal network on pre breeding.

Organize a few days meeting for participantsith l i t t ( ll 3 WG i l d d) ith th i twith real interest (all 3 WGs included) with the aim to

define targets and funding possibilities.

Key words: climate change, emerging diseases, cereals, pre-breeding, base-broadening

ECPGR Cereals Network Working Groups on Barley

Project proposal for ECPGR Phase VIII

“ACCELERATED PRE-BREEDING FOR CLIMATIC CHANGE”

Original workplanOK:s• Survey on activities of the project participants• Kick-off meeting• Knowledge sharing

Still waiting on the ECPGR level• Evaluation of germplasm, extending the present ring tests (or including them in the pre-breeding initiative)in the pre-breeding initiative) • Inventory of pre-breeding activities in Europe• Identification characterisation and evaluation data required Evaluation of on-going SNP genotyping of germplasmgoing SNP genotyping of germplasm • Identification of priorities for additional genotyping• Starting the development of pure genetic stocks as ‘type specimens’ of gene-bank accessions• Starting the pre-breeding programmes

Timetable2008 ( f ) 2009 2009•Meeting 2008 November–December (preferably), or early 2009 late 2009

•External project proposal(s) after the meeting

Seminar

• A pre-breeding workshop on cereals in Alnarp Sweden on 24-25 November 2009.• Organized by NordGen, ECPGR, SLU, Graminor, MTT Agrifood Research O g y , C G , S U, G , gFinland and Oatly. • Altogether 49 participants from 12 countries joined the first meeting on increasingly important subject on pre-breeding.•The programme included 20 presentations with four current themes: 1) Pre-breeding in cereals – status and examples of existing collaborations, 2) Breeding goals and needs, 3) Challenges of funding and 4) Future needs and

li tiapplication.

• Conclusions from discussions made common interests were prioritized• Conclusions from discussions made – common interests were prioritized• A Concept Notes on Pre breeding issues was produced – available on webto everyone’s use

Further activities – Updating the WG’s PreBreeding Work Plan(to be discussed in discussion groups)(to be discussed in discussion groups)

• Elcio’s thoughts:1) Activities related to PGR: high cost and long term return 2) L k f h t i ti d l ti d t2) Lack of characterization and evaluation data 3) Knowledge on the genetic diversity, local knowledge and diversity important –information from them, knowledge of the inter species relationship 4) Encourage the development of public-private cooperation) g p p p p5) Prebreeding needs a strong breeding program to operate under

• Conclusion paper from seminar1) Build a joined disease resistance database including for ex data on PGR reactions to1) Build a joined disease resistance database including for ex. data on PGR reactions to different biotic/biotic stresses, and the availability of molecular markers. The database can also be used as a tool to identify missing data and the need for urgent research. It was also discussed that there is a need for access to joined differential sets and nurseries for the various diseases of importance.2) A need to develop phenotype tests relevant for abiotic stress resistance and apply to relevant germplasm3) Lobbying for pre-breeding at EU and national levels and supporting establishment of3) Lobbying for pre-breeding at EU and national levels, and supporting establishment of private-public collaborations

Further activities(to be discussed in discussion groups)(to be discussed in discussion groups)

4) Increase the online availability of characterization/evaluation data,combine the FIGS concept and evaluation data to develop precise models 5) I th di l b t b k d b d t i f t d5) Improve the dialog between gene banks and breeders, strive for transparency and build trust in the legal frameworks6) Make genotyping information available where differences in genome regions for adapted material vs. landraces are demonstrated. p7) The policy needs to be in line with political agenda and decision makers, and on the EU level, awareness on research needs should be raised.

• What about:• What about:1) AEGIS / pre-breeding interaction?2) The role of ECPGR: deciding on common European specific aims / applying for funding / connecting people / organizings seminars to share the knowledge / / etc, etc…

As a result of the meeting:As a result of the meeting: AN UPDATED, CONCRETE, REALISTIC WORKPLAN ON PRE-BREEDING

With specified aims, outcome, people in charge, timetable

Updating the WG’s PreBreeding & breeding Work PlanUpdating the WG s PreBreeding & breeding Work Plan

1) Agree on activities without additional funding

2) Agree on project plan aiming at funding

• Build a joined disease resistance database among participants• Build a joined disease resistance database among participants• including for ex. data on PGR reactions to different biotic/biotic stresses• information on availability of molecular markers • information on differential sets and nurseries for the various diseases of importance• Combine the FIGS concept and evaluation data to develop precise models• Person resp: Nordforsk project among Nordic countries• Timetable:Timetable:

• Develop/describe phenotype tests relevant for abiotic stress resistance• Person resp.: MS• Timetable:

• Evaluation of germplasm (ring tests)• Person resp : MJ• Person resp.: MJ• Timetable:

• Lobby/market for pre-breeding at EU and national levels, and support establishment of private-public collaborations (concept note)

• Person resp.: everyone

• Distribute the questionnaire

EVALUATION* RING TESTDEVELOPMENT OF

MOLECULAR MARKERSDrought resistanceResistance to abiotic stressesResistance to biotic stresses

*

Resistance to biotic stressesResistance to RamulariaResistance to RhynchosporiumResistance loose&covered smutResistance to leaf stripeResistance to Pyrenophora teresResistance to Puccinia hordeiAmino acid compositionBeta glucan contentBreeding for qualityDetermination of short day genesDetermination of transpiration indexDetermination of transpiration indexDifferent climatic conditionsDomestication of barleyGenetic diversity studiesHullesMineral use efficacyPh i l i l & h l i l tt ib tPhysiological & morphological attributesPhytate contentStarch charactersTreshing ability

*landraces, wild species, genetic stocks, mutants, cultivars * Wild species, landraces, genetic stocks, mutants, breeding material, cultivars p , , g , , g ,

• Project proposal• Project proposal

• Key’words’:- Climate changeg- Use of Genetic Resources (new alleles)- Produce Genome-based resources to support molecular breeding- Quality / resistance traits for phenotyping

Crop physiology- Crop physiology- Advanced Agronomy- Bioinformatic tools- Integration of the actors of the Barley chain- Dissemination of the new materials and technologies

• Lorenzos project and/or Individual project

• Persons resp :Persons resp.:• Timetable:

• Distribution of information• e-mail, home page• meetings (also others than ECPGR)meetings (also others than ECPGR)• Persons resp.: everyone• Timetable: continuous