PRACTICAL

MICROBIOLOGYGram’s stain

Gram’s stain

Gram staining (or Gram's method) is a method of

differentiating bacterial species into two large groups

Gram Positive Gram negative

Gram staining differentiates bacteria by the chemical and

physical properties of their cell walls.

Dyes and chemicals used in staining

What happens in staining process!!!

• 1. smear preparation

Procedure

Staining procedure

Results

Examples

Gram –ve bacilli Gram +ve cocci