practical biotechnology concept
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Biotechnology-Practical Lab.1 3rd Student-Medical Analysis 2021
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Practical Biotechnology Concept
o Biotechnology is a field of applied biology that involves the use of living
organisms or parts of organisms for practical uses and bioprocesses in
engineering, technology, medicine and other fields requiring bioproducts.
o Modern use similar term includes genetic engineering as well as cell- and
tissue culture technologies.
o , as ‘the appliance of bioscience’, is helping to
diagnose and treat major human diseases.
provide solutions for a cleaner environment.
produce new industrial bioproducts and processes.
o is a rigorous course that
develops problem-solving skills necessary for success doing science at the
bench while exploring in depth the chemistry of biological molecules.
o Knowledge in this area is directly applicable to the biotechnology fields of
pharmaceuticals, environmental processes and remediation, as well as
bioinstrumentation.
Biotechnology-Practical Lab.1 3rd Student-Medical Analysis 2021
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LABORATORY SCHEDULE (Syllabus):
Weeks Lab. Topic
Week 1 Basic Concept and Instruction about Practical Biotechnology Lab.
Week 2 Nucleic Acid (DNA and RNA) Melting Point Determination Lab.
Week 3 Agarose Gel Electrophoresis Methods
Week 4 Polyacrylamide Gel Electrophoresis Analysis
Week 5 Polymerase Chain Reaction(PCR): Its Programs and Protocols of
Running it
Week 6 Finger Printing and Real Time PCR
Week 7 Primers Designs and Their Preparations (Finding Out What
Bioinformatics Can Do for You?)
Week 8 Restriction Enzyme Analysis
Week 9 DNA Ligase Analysis
Week 10 Bacterial Transformation (Competent Cells)
Week 11 Bacterial Conjugation
Week 12 Fluorescent in situ Hybridization Techniques and Southern
Blotting Techniques (Blotting)
Week 13 Western Blotting Technique
Week 14 Northern Blotting Technique
Week 15 Flow Cytometry (FACS) Protocols
Week 16 Gene Analyzer and DNA Sequencing.
Week 17 Chromatography (Paper Chromatography)
Week 18 Column Chromatography and Thin Layer Chromatography
Week 19 Separation of Enzymes and their Determination Using ELISA and
Cobas e400 plus Technique
Week 20 Separation of Vitamins and their Determination the Immunoassay
Analyzer Cobas e411 Technique