ppt mikroum fixed
TRANSCRIPT
GROUP 2:
Angga Fatchulloh A. (105090100111009)
Khairatul Insani F. (105090100111031)
Dharma Asih P. (105090101111009)
Lia Rachmawati (105090107111001)
Winda Rahayu (105090113111001)
The growth of every living creature needs sufficientnutrients and environmental conditions that support for thegrowth process, as well as microbes. There are severalfactors that can affect the growth of microbes, such as:temperature, humidity, osmotic pressure, light, pH, and AWnutrient. Antibiotic resistance is a bacteria that has a set ofways to adapt for environments that contain antibiotic.Resistance mecanism include mutations in bacteria,inhibition of the enzymatic activity of antibiotics, changes inproteins that are targets of antibiotics, changes in metabolicpathways and membrane permeability
Student lab can determine the effect of
hydrogen’s ion concentration on the growth
of microbes
Student lab can distinguish the nature of
microbes based on their oxygen needed
Student lab can determine the effect of
chemical compounds on the growth of
microbes
pH 5pH 3 pH 7 pH 9
Physiological pH
Flacon tube with 5 ml
bacteria culture isolates 24
hours
Inoculated into tubes of
glucose phenol red broth
pH indicator, four tubes
should each contain 1 ml
bacteria culture isolates
48 hours of incubation at
room temperature
Physiological oxygen
48 hours of incubation
at room temperature
Melt the nutrient agar by
steaming, cool to 45
degrees Celcius
Inoculated 0.1 ml bacteria
culture isolates into tubes
of nutrient agar
homogenization
Inoculated by spread plate
technique 0.1 ml bacteria
culture isolates into plate of
nutrient agar
Flacon tube with 0.9 ml
bacteria culture isolates 24
hours
48 hours of incubation
at room temperature
Resistance of antibiotic
Fixing four antibiotic discs
to plate of nutrient agar with
the use Laminar Air Flow
(LAF)
Pict. 1. Tubes which contain of 5 ml Glucose-phenol red broth with power of
Hydrogen (pH) 3,5,7,9 before inoculation and incubation.
2a. 2b. 2c. 2d.
From the pictures above, we know that all of the tubes are shown positive reaction after
incubation by isolates. Pict: 2a. pH 3 2b. pH 5 2c. pH 7 2d. pH 9
Positive reactions: not only indicated by the color change on Glucose-phenol broth but
also can be the formation of a precipitate in the bottom of tube.
Colour after Inoculation Colour after Incubation Growth On pH
3 5 7 9 3 5 7 9 3 5 7 9
Yellow Yellow
orang
e Red Yellow yellow Dark orange Dark red - - + +
Yellow Yellow
orang
e Red Yellow orange Red DarkRed + + + +
Yellow Yellow
orang
e Red Yellow
slightly
turbid yellow
slightly turbid
red
slightly turbid
yellow, there are
deposits + + + +
Yellow Yellow
orang
e Red Yellow Yellow
slightly turbid
red turbid red - - + +
Yellow Yellow
orang
e Red
slightly
orange
orange in
surface
orange in
surface Orange + + + +
Yellow Yellow
orang
e Red Yellow turbid yellow turbid yellow Jingga - + + +
Yellow Yellow
orang
e Red Yellow turbid yellow Yellow Light Orange - + + +
Yellow Yellow
orang
e Red Yellow Yellow Light Orange Light Orange - - + +
Microbial
K2C1
K2C2
H1K2
H3K3
H4K2
Eschericia coli
Bacillus cereus
Salmonella sp.
Table 1. Result of pH’s Effect on Microbial Test
3a. 3b.
Pict. 3a.Tubes which contain of 10-15 ml Nutrient agar before inoculation
and 3b. After inoculation (growth of aerob bacteria)
Pict.4 positive reaction is indicated by clear zone
formation on the medium.
Cephazoline (+)
Kanamycin (+)
Chlorampenizol (+)
Erythromycin (+)
The growth of bacteria is affected by pH, oxygen
and chemicals
There are several kinds of microbial based on
their need for oxygen: aerobic, facultative
anaerobes and obligate anaerobes
The effect of chemical compounds can inhibit the
growth of microbes