Abstracts / Journal of Biotechnology 185S (2014) S37–S125 S65

following 24 and 48 h. In conclusion, TDCPP was able to induceddetectable cytotoxic effects on HeLa cells. Na+-K+-ATPase can rep-resent one of the potential cellular targets of the FR toxic action.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.218

Assessment of difenoconazole effect on soilmicroorganisms based on enzymatic analyzesunder different temperature conditions

Marioara Nicoleta Filimon 1,∗, Adriana Isvoran 1,Diana Vladoiu 2, Roxana Popescu 3, Doina Verdes 3,Daliborca Vlad 4, Sorin Voia 5, Vasile Ostafe 2

1 Department of Biology-Chemistry, West University,Timisoara, Romania2 Laboratory of Advanced Researches inEnvironmental Protection, West University,Timisoara, Romania3 Department of Cellular and Molecular Biology,University of Medicine and Pharmacy “Victor Babes”,Timisoara, Romania4 Department of Biochemistry and Pharmacology,University of Medicine and Pharmacy “Victor Babes”,Timisoara, Romania5 Faculty of Animal Science and Biotechnology, BanatUniversity of Agricultural Sciences and VeterinaryMedicine, Timisoara, Romania

E-mail address: nicoleta filimon@yahoo.com (M.N. Filimon).

Difenoconazole (3-clor-4-[(2RS,4RS,2RS,4SR)-4-metil-2-(1H-1,2,4-triazol-1-il-metil)-1,3-dioxolan-2-il]fenil4-clorfenil eter) isa broad-spectrum fungicide frequently used in crops to destroypathogen fungi. Difenoconazole treatment was carried outunder laboratory conditions, at 28–29◦ C, at concentrationsof 0.037–0.150 mg/g soil. Biochemical parameters deter-mined during a 21 days period after the fungicide treatmentwere dehydrogenase and urease activities. Dehydrogenaseactivity in soil samples treated with three different doses ofdifenoconazole, registered values of 0.497 ± 0.107 mg triphenyl-formazan/g soil, 0.352 ± 0.159 mg triphenyl-formazan/g soil and0.413 ± 0.339 mg triphenyl-formazan/g sol. The values of ureaseactivity in experimental samples were 2.720 ± 0.691 mg HN4/gsoil, 2.491 ± 0.701 mg HN4/g soil and 2.388 ± 0.569 mg HN4/gsoil. Comparing dehydrogenase and urease activities to the val-ues registered in the untreated soil sample we could observe astatistically significant decrease p < 0.05. Based on dehydroge-nase activity we can conclude that a temperature of 28–29 ◦Chas a stimulatory effect on the growth and metabolism of soilmicroorganisms, inducing probably a high metabolisation rateof difenoconazole. This work was supported by a grant of theRomanian Ministry of Education, CNCS-UEFISCDI, project numberPN-II-RU-PD-2012-3-0220.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.219

Construction of microbial biopreparations foroil fields waste cleanup in the Republic ofKazakhstan

Azhar Zhubanova 1, Gulzhan Kaiyrmanova 1,Dariya Jussupova 2, Aliya Yernazarova 1,∗

1 Department of Biotechnology, Al-Farabi KazakhNational University, Almaty, Kazakhstan2 Department of Geography of Kazakhstan, Ecologyand Methods of Teaching, Abai Kazakh NationalPedagogical University, Almaty, Kazakhstan

E-mail address: aliya.yernazarova@gmail.com (A. Yernazarova).

Intensive oil fields development in Kazakhstan has increased thelandfills for disposal of waste oil, i.e. it led to an increase of thecontaminated land area and, second, respectively, to the severeenvironmental problems in the region of oil production, and inthe surrounding areas as well. Oil waste disposal requires inte-grated approaches and includes physical, chemical and biologicalmethods. As is well known, bioremediation using hydrocarbon-oxidizing microorganisms remains to be the most promising way torestore the productive soil properties. In this report we present theresearch results on designing of effective biopreparations based on10 strains of indigenous microorganisms isolated from the liquidand solid soil samples of investigated region and 2 strains of theuniversity department collection of microbial cultures. Based onthe study results of the oil emulsification index and assess of biolog-ical stability to the landfill drilling storage wastewater, the 3 mostactive destructor-cultures of Zhanazhol oil waste deposits wereidentified. Based on the summary of research work, the microor-ganisms association consisting of 2 microorganism cultures wasrecommended for further field remediation means: Pseudomonasssp. BShS-1 and P. aeruginosa H14.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.220

Potential of microbes to decompose crude oilcontaminated soil

Virgil Scarlat 1,∗, Maria Pele 1, Elena MariaDraghici 2

1 Department of Biotechnologies, University ofAgronomic Sciences and Veterinary Medicine,Bucharest, Romania2 Department of Horticulture, University ofAgronomic Sciences and Veterinary Medicine,Bucharest, Romania

E-mail address: mpele50@yahoo.com (V. Scarlat).

Introduction: One of the serious problems of our time is increasinglyfrequent oil pollution. Therefore has become an alternative way ofremediation of oil contaminated sites through the addition of spe-cific microorganisms. The main aim of this study was evaluating theeffect of some microorganisms on oil polluted soils samples. Mate-rials and methods: Contaminated soil samples were taken from 5different polluted areas from Romania. The soil samples were airdried and portions of 5 kg were placed in special containers, inoc-ulated with different microorganisms collected from soils pollutedwith crude oil. Determination of total petroleum was carried outaccording by gravimetric method. Results: Amounts of TPH fromsoils analyzed are 14.11 to 41.73 times higher than the highest valueallowed for a soil with low usage. The most effective decompositionof petroleum products was shown by consortia of fungus Fusariumand Aspergillus. After 21 days the content of oil in these samples isalmost zero. Conclusions: This study showed that identify the most

S66 Abstracts / Journal of Biotechnology 185S (2014) S37–S125

effective microorganisms for decontamination from polluted soils,multiplying and reintroduction in soils affected greatly assist theremediation process. The results suggested complementary studiesfor separation and detection of effective microorganisms of pol-luted soils.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.221

Antimicrobial activity of Humulus lupulusextract

Steliana Rodino 1,3,∗, Alina Butu 1, ConstantaNegoescu 2, Petruta Petrache 3, Reta Condei 4,Ioana Nicolae 4, Calina Petruta Cornea 3

1 National Institute of Research and Development forBiological Sciences, Bucharest, Romania2 Banat’s University of Agricultural Sciences andVeterinary Medicine “Regele Mihai I al României”from Timisoara, Romania3 Faculty of Biotechnology, University of AgronomicSciences and Veterinary Medicine of Bucharest,Romania4 Faculty of Management, Economic Engineering inAgriculture and Rural Development, University ofAgronomic Sciences and Veterinary Medicine ofBucharest, Romania

E-mail addresses: steliana.rodino@yahoo.com (S. Rodino),alina butu@yahoo.com (A. Butu).

The research on naturally occurring substances presenting antimi-crobial properties has intensified in the last decades due to legalregulations and also to the consumers demand that request toreduce the quantities of chemical products used on horticulturalcrops. In this paper was investigated the inhibitory activity ofHumulus lupulus L. (hops) extract against some plant pathogens,represented by: Alternaria spp, Pythium debarianum and Phytoph-thora infestans. The plant extract was obtained by cold percolationfrom dried and minced female inflorescences. The poisoned foodtechnique was used for antimicrobial activity evaluation, by using aconcentration of 2%, 4% and 8% of the extract in the growth medium.Although the response of the tested fungi to each concentration wasdifferent over the control, generally growth inhibition increasedwith the concentration, on the tested range. The in vitro studiesrevealed that the concentration of 8% hops extract showed fungi-toxic activity against all the strains tested.

AcknowledgementsThis work has been funded by UEFISCDI research contract PN-

II-PT-PCCA 106/2012.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.222

Expansion of the use of natural reeds(Phragmites australis) in the delta river Ili

Ruslan Salmurzauly ∗, Sabir Nurtazin, ZuraYessimsiitova, Zhannat Bazarbaeva

Department of Biology and Biotechnology, Al-FarabiKazakh National University, Almaty, Kazakhstan

E-mail address: ruslaan200587@gmail.com (R. Salmurzauly).

Due to the sharp decline of state support for the agricultural sectorin many regions of Kazakhstan, including the delta river Ili, therewas a sharp recession of production, increased pressure on naturalecosystems as the result of poaching, uncontrolled haymaking, pri-vate fires, deforestation. Therefore is necessary search of economic

solutions to ensure maximum preservation of natural ecosystemsincompliance with the requirement to reduce the load to the envi-ronment. One of the most promising options of land use in the deltaIli ensuring sustainable preservation of the environment is use ofthe reed, which stocks in 2010 was 338,400 ha; with an averageyield about 200 centner/ha. In this region the reed (Phragmites aus-tralis) has the highest potential growth for seasonal phytomass, andwe can use it as the fuel granules and as the feeding briquettes withthe minimal expenses for manufacturing of raw material. Plannedto purchase of special equipment to manufacturing the reed gran-ules and briquettes. The reed certainly applies to renewable energyand its efficient use can provide a saving coal, as well as promoteconservation saksaul forest which traditionally used by local peopleas fuel.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.223

Influence of unsymmetrical dimethylhydrazineand nitrosodimethylamine to growth indicatorsof the wheat

Saltanat Usubalieva 1,∗, Elmira Boribay 1, IntykShayahmetovna Shayakhmetova 2, OrynbasarTleuberlina 1, Nagima Muhazhanova 1, NursuluAkhtayeva 3

1 Kazakh Economic University named afterT.Ryskulov, Almaty, Kazakhstan2 Almaty Technological University, Almaty,Kazakhstan3 Al-Farabi Kazakh National University, Almaty,Kazakhstan

E-mail address: usubalieva.saltanat@mail.ru (S. Usubalieva).

UDMH (in 0.1 N HCI) and NDMA in concentrations – 10−6 M, 10−5 M,10−4 M, 10−3 M were taken for research. The seeds of the wheatvarieties Omsk-9 were germinated in solutions of the test sub-stances in the dark at a temperature of 22–240 ◦C. The results ofresearch showed braking of germination of grains when exposedto large concentrations of the test substances. Inasmuch as UDMH(10−3 M) reduces the degree of germination of the wheat on 15%,while at the same NDMA concentrations causes complete brakingof germination of the grain. It should be noted that UDMH notvery affect to the neoplasm of the roots, however NDMA exert aninhibitory effect. The degree of braking of root growth depends onthe concentration substance. Thus, in the processing with solutionsof 10−5 M, 10−6 M UDMH new roots appeared on 20–32% and athigh concentrations on 16–18% less. It should be noted that theroot formations is amplified to the level of control when movingplants all of the options into water and the growth in length isreversed only partially. Also, the dependence of growth of the aerialparts of the concentration of hydrazine was investigated. NDMA ascompared with UDMH more strongly inhibits growth.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.224