7/25/2019 Poster Dfbs

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INFLUENCE OF CADMIUM, LEAD AND NICKEL ONPHOTOSYNTHETIC APPARATUS OF BLACK LOCUST

(Robinia pseudoacaciaL. 1753)Milan upunski1, Milan Boriev1, Saa Orlovi2, Slobodanka Pajevi1, Nataa Nikoli1,

Andrej Pilipovi2, Rita Horak11University of Novi Sad, Faculty of Sciences, Department for Biology and Ecology, Serbia2University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia

Introduction Plantsrepresents anideal agents for soil and water remediation because of their unique genetic, biochemical and physiological properties.Black locust is characterizbiomass productive species. It is a native tree to southeastern America, and due to his rapidgrowth and its capacity to fix atmospheric nitrogen, it has been used in rehabmining land and heavily industrialized areas.The occurrence of heavy metals in soils may be beneficial or toxic to the environment. Theyare extremly dangerous for planconcentrationsbecause of its cumulative destructive effect, but the most dangerous effects areexpressedby combinations of several heavy metals. The aimof this stuexamine the influence of cadmium, nickel and lead to photosynthetic activity of black locust, and determinethe ability for phytoremediationapplication.

Material and Methods Photosynthesis and transpiration were measured using the LCpro+ portable photosynthesissystem, manufactured by Adc BioScientific Ltd.Light conditions were set using the LCpro+ light unit,which emitted photosynthetically active radiation (PAR) at 1000 mol m-2s-1. The air supply unitprovided a flow of ambient air to the leaf chamber at a constant rate of 100 mol m-2s-1. Humiditywere maintained at rate of 10 mbar. Temperature and CO

2concentration were at ambient levels.

Parameter WUE (water use efficiency) was calculated as the ratio of photosynthesis to transpiration. The experiment included a control group of plants and five treatments of pollution:Pb, Cd, Ni

and two mixed treatments (Mandm) of all three metals in two concentrations. Concentrations of Cd,Pb and Ni were determined from prepared solutions by employing atomic absorptionspectrophotometry (AAS). All data were analyzed using Duncans multiple range test at the level of significance p