points in practical virology

Important points in practical virology

Abdou Mohammad Nagy, DVM, PhDDepartment of Virology

Faculty of Veterinary MedicineZagazig University, Zagazig 44519, Egypt

Biological safety cabinetsClass I biological safety cabinet

http://www.phac-aspc.gc.ca/publicat/lbg-ldmbl-04/ch9-eng.php

Class II biological safety cabinet


Class III biological safety cabinet


Work under BSC

Tissue homogenizer Ice box Ice pack

Pestle & mortar

What is Positive pressure protective suit (PPPS)?

Fresh, filtered air is typically supplied to the interior of a PPPS via overhead tubing. In addition to the physical barrier provided, the positive pressurization offers additional protection in the event of exposure through a defect or puncture in the suit, for if the suit’s integrity is compromised, air will be forced out instead of being sucked in. Extensive training with the PPPS is required to safely pursue research in a PPPS within a biosafety facility.

https://en.wikipedia.org/wiki/Positive_pressure_personnel_suit




Do you want to work with Ebola virus?

http://www.naturalnews.com/047226_Ebola_outbreak_Dallas_health_care_worker.html




Could you Identify improper biosafety problems?

Common safety practices

https://www.google.com.eg/search?q=biosafety+symbols

https://www.google.com.eg/search?q=biosafety+symbols

How do you use fire extinguisher?

http://www.fireonline.com.au/extinguishers

Types of fire extinguisher

http://www.fireandsecurity-alarms.co.uk/fire-extinguishers/



Liquid nitrogen hazards in virology lab

University of Windsor students, overseen by Andrzej Sobiesiak, the head of the mechanical engineering department, right, transport a tank designed to carry 200 litres of liquid nitrogen along Wyandotte Street http://windsorstar.com/news/local-news/probe-launched-after-students-roll-hazardous-material-down-street

http://windsorstar.com/news/local-news/probe-launched-after-students-roll-hazardous-material-down-street

http://windsorstar.com/news/local-news/probe-launched-after-students-roll-hazardous-material-down-street

Evaporated Liquid Nitrogen-Induced Asphyxia: A Case Reporthttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2526498/

Petechiae (arrowheads) were revealed in periorbital areas of the decedent.

What does your lab need to detect high levels of liquid N2??

Virus inoculation in fertile eggs

Good vs bad eggs

Virus Inoculation in fertile eggs

A) Curled and dwarfed embryos post IBV infection. B) Normal embryohttps://www.researchgate.net/publication/285581786_Phylogentic_analysis_of_recent_infectious_bronchitis_virus_isolates_from_broiler_chicken_farms_in_Kafrelsheikh_Egypt/figures?lo=1

Pock lesions https://en.wikipedia.org/wiki/Chorioallantoic_membrane#/media/File:Smallpox_CAM.png

https://www.researchgate.net/publication/285581786_Phylogentic_analysis_of_recent_infectious_bronchitis_virus_isolates_from_broiler_chicken_farms_in_Kafrelsheikh_Egypt/figures?lo=1






https://en.wikipedia.org/wiki/Chorioallantoic_membrane#/media/File:Smallpox_CAM.png



Mouse inoculation (I like it)Handling of mice

http://www.usp.br/bioterio/Artigos/Procedimentos%20experimentais/Routeadministration-4.pdf

S/C inoculation


Intracerebral inoculation


Intraperitoneal inoculation Intranasal inoculation


Intravenous inoculation

Cell culture equipment Inverted microscope

Roux bottleLeighton culture tube

Primary cell culture preparation

http://www.cloud-clone.com/topic/Primary-cell-culture.html



Cell subculture, freezing & Thawing

https://www.abmgood.com/marketing/knowledge_base/cell_culture_introduction.php



CPE produced by viruses

http://abbs.oxfordjournals.org/content/41/12/998/F2.expansion



http://www.virology.ws/2009/05/28/influenza-microneutralization-assay/



What is the difference between two images?

Different pH of cell culture medium

Pink YellowRed

Thank you

points in practical virology

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