‘Isolation of DNA’ classes at the University of Gdansk

On 22th April class II A took part in biology classes at the University. The subject of this project was isolating DNA from kiwi fruit.

Introduction

When we came into the lab we were greeted by a lecturer. Then we put our aprons on and the scientist told us about laboratory equipment which we used.

In the lab

Laboratory equipment which we used

Vortex Separator Electronic pipette

Next, the teacher told us how to use GENOMIC MINI AX TISSUE - special set to isolate DNA.

In the lab

Column

Receiving tube

Ion exchange membrane binding DNA

The flow of liquid under the influence of gravity and capillarity

DNA isolation consisted of passing through the membrane reagents and sample organic with a special fluid and mixing in special separator.

In the lab

At the end we had to remove water and residual liquids from the sample.

In the lab

We could take ready samples to houses- (samples were ready to watch next day).

In the lab

After classes

The process of DNA isolation is very arduous, but students were very pleased with these classes, because they could use the professional laboratory equipment.

Thanks for your attentionThanks for your attention

Sources:

• http://wtz.ar.krakow.pl/kbz/wirowki.html• http://www.meranco.com.pl/produkty/pipeta-elektroniczna-xplorer• http://www.wirowka-laboratoryjna.adverti.com.pl/item.php?id=WIRLAB-MPW-

031&nazwa=Vortex_BIOSAN_V-1_plus• http://www.aabiot.com/gravity/• http://www.scind.org/82/Mindblower/russia-to-build--worlds-first-dna-databank.html• https://aphroditessecretcyprus.com/?product=kiwi-dried• http://www.fototerytorium.pl/14084-info-probowki-z-kolorowym-plynem-w-

drewnianym-stoja.html• http://www.conrad.pl/Mikroskop-Bresser-Optik-USB,-powi%C4%99kszenie-40x-

1024x.htm?websale8=conrad&pi=825709

XX Secondary School in Gdańsk, PolandSupervision: Agnieszka Jackiewicz

Consultation: Beata PiełowskaAuthor: Maciej Suchocki