Please form a line in order to get the materials that are

needed for the day.

Microscope Parts & Function

Electron Microscope Light Microscope

• Ocular Lens (eyepiece) magnifies the image 10X

• Body Tube – distance between eyepiece & objectives.

• Arm – supports nosepiece

• Objectives – further magnifies the image. – 4X– 10X– 40X

• Revolving Nosepiece – holds the objectives, turns to change from one objective to another

• Stage – supports slide

• Stage Clips – secure the slide in position for viewing

• Coarse Adjustment Knob – moves the stage up and down in large increments to bring image into a rough focus.

• Fine Adjustment Knob – moves the stage slightly to bring image into sharp focus.

• Diaphragm – controls the amount of light that is allowed to pass through the object being viewed

• Light Source – provides light for viewing the image

• Base – supports microscope

• X axis stage adjustment – moves stage (and image) left & right.

• Y axis stage adjustment – moves stage (and image) up & down.

Microscope Use

Human Hair - 200X

Human Skin – 50X

Spider – 40X

Flea – 40X

Spider – under electron microscope

Flea under electron microscope

Deer hair (800X) electron microscope

Skin – electron microscope

1. Make sure the 4X (low power) objective is in place, and use the coarse adjustment knob to lower the stage to as low as possible.

2. Place a slide on the stage and stabilize it with the clips.3. Center it over the light source using the x- and y- adjustment knobs. 4. Slowly decrease the distance between the stage and the nosepiece using the coarse

adjustment knob until the object comes into focus.5. It may now be necessary to rotate the diaphragm to allow in the appropriate amount

of light (generally, thinner specimens will need less light and thicker specimens will need more).

6. Use the fine adjustment knob to sharpen the focus. 7. Once the object is in focus under the low power (4X) objective, make sure that the

object is COMPLETELY centered in your field of view using your x- and y-adjustment knobs (the object is directly at the end of the black line in your field of view).

8. Move to the next highest powered objective. 9. From this point forward, use only the fine adjustment knob to sharpen focus. 10. When finished observing the specimen, rotate the nosepiece so that the 4X (low

power) objective is back into place. Use the coarse adjustment knob to lower the stage back to its lowest point. Remove the slide.