plasticity of fiber type expression objectives (i and ii) methodology: methodology:...
TRANSCRIPT
![Page 1: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/1.jpg)
PLASTICITY OF FIBER TYPE PLASTICITY OF FIBER TYPE EXPRESSIONEXPRESSION
OBJECTIVES (I and II)OBJECTIVES (I and II)•Methodology:Methodology:
• Electrophysiology: resting Em and action Electrophysiology: resting Em and action potential potential
• Measurement of ion channel activityMeasurement of ion channel activity• Source of informationSource of information
•Role of Role of ::• CalcineurinCalcineurin• nFATnFAT• Nitric oxide (NO)Nitric oxide (NO)• NOR-1NOR-1
![Page 2: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/2.jpg)
INTRODUCTIONINTRODUCTION
![Page 3: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/3.jpg)
CALCINEURIN
CATALYTIC DOMAIN
CaM-Bindingdomain
Auto-InhibitoryDomain
Calcineurin A (CNA)
![Page 4: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/4.jpg)
CALCINEURIN AND NFAT PATHWAY IN B-LYMPHOCYTES
![Page 5: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/5.jpg)
CALCINEURIN-NFAT Pathway
![Page 6: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/6.jpg)
CALCINEURIN INCREASES mRNA LEVELS FOR MYOGLOBIN, SLOW TnI AND SARCOMERIC MITOCHONDRIAL CREATINE
KINASE IN GASTROCNEMIUS MUSCLE
![Page 7: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/7.jpg)
CALCINEURIN DECREASES mRNA LEVELS FOR PARVALBUMIN AND MUSCLE CREATINE KINASE
IN GASTROCNEMIUS MUSCLE
![Page 8: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/8.jpg)
FIRING PATTERNS IN MUSCLE
![Page 9: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/9.jpg)
FIRING PATTERNS IN MUSCLE
![Page 10: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/10.jpg)
FIRING PATTERNS vs. FORCE-FREQUENCY CURVE IN MUSCLE
![Page 11: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/11.jpg)
Ca2+ TRANSIENT IN SLOW AND FAST FIBERS
![Page 12: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/12.jpg)
DISCUSSIONDISCUSSION
![Page 13: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/13.jpg)
ACTIVATION OF NF-ЌB, JNK1 AND NFAT IN B-LYMPHOCYTES
![Page 14: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/14.jpg)
ACTIVATION OF NF-ЌB, JNK1 AND NFAT IN Ca2+ spike vs. prolonged
elevation
![Page 15: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/15.jpg)
ACTIVATION OF NF-ЌB, JNK1 AND NFAT IN small prolonged elevation of
Ca2+
![Page 16: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/16.jpg)
ACTIVATION OF NF-ЌB, JNK1 AND NFAT IN Ca2+ dose-response curve
![Page 17: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/17.jpg)
MUSCLE OVERLAOD CAUSES A CALCINEURIN DEPENDENT
HYPERTROPHY
![Page 18: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/18.jpg)
MUSCLE OVERLAOD CAUSES A CALCINEURIN DEPENDENT INCREASE
IN TYPE I FIBERS
![Page 19: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/19.jpg)
MUSCLE OVERLAOD CAUSES A CALCINEURIN DEPENDENT CHANGE IN mRNA OF MHC AND
TnI
![Page 20: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/20.jpg)
h
![Page 21: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/21.jpg)
![Page 22: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/22.jpg)
h
![Page 23: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/23.jpg)
h
![Page 24: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/24.jpg)
![Page 25: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/25.jpg)
h
![Page 26: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/26.jpg)
h
![Page 27: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/27.jpg)
h
![Page 28: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/28.jpg)
h
![Page 29: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/29.jpg)
h
![Page 30: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/30.jpg)
![Page 31: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/31.jpg)
h
![Page 32: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/32.jpg)
h
![Page 33: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/33.jpg)
![Page 34: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/34.jpg)
h
![Page 35: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/35.jpg)
h
![Page 36: PLASTICITY OF FIBER TYPE EXPRESSION OBJECTIVES (I and II) Methodology: Methodology: Electrophysiology: resting Em and action potential Electrophysiology:](https://reader035.vdocuments.us/reader035/viewer/2022081603/56649eb15503460f94bb77e2/html5/thumbnails/36.jpg)
h