HARIS PMSC BIOTECHNOLOGYM.A.COLLEGEKOTHAMANGALAM

ISOLATION OF PLASMID DNA BY ALKALINE LYSIS WITH SDS

• Plasmid is an extra chromosomal DNA molecule which is capable of self replication.

• It is circular and double stranded.

• Plasmids naturally occur in bacteria but are also found in eukaryotic yeast.

HOW PLASMIDS ARE ISOLATED???

3 MAIN STEPS:-

1. CELL LYSIS

2. DEPROTEINATION & REMOVAL OF Chr.DNA

3. PRECIPITATION OF PLASMID DNA

FOR WHAT ????