plasma surface treatment and polymerization for functionalizing material surfaces jw bradley dept....
TRANSCRIPT
Plasma surface treatment and polymerization for
functionalizing material surfaces
JW Bradley
Dept of Electrical Engineering and Electronics
The University of Liverpool
1) Remove material
1) Add material
1) Change chemical or physical nature of the surface
Incident particle
Sputtered particle
Atomic collisions
Plasma surface treatment of polymers
Environmental advantages over conventional processes
bullEnhanced adhesion eg automotive - car bumpers
bullEnhanced wetability
bullSurface preparation for cell support
bullBio-compatibility - lens treatment
bullTextile treatment
bullMicro-electronics
PC
Mass SpectrometerProbe
HAL EQP 1000 Mass Spectrometer
Controller
Pirani gauge
Rotary Pump
Monomer vapour inlet
Substrate
OscilloscopePulse Generator
Matching Network
RF Generator and Power Meter
Turbo Pump
Glass chamber
LOW-PRESSURE PLASMA
Cell growth and viability on patterned surfaces
MG63 cells on plasma patterned PS after 48 hours culture The gaps width 200 m
5m
10m
AFM Friction image (O+N)C = 027
With RD Short ndash Sheffield
Controlling ion energy and flux
XPS analysis of the surface modification of polystyrene
0
3
6
9
12
15
18
0 5 10 15 20 25 30 35
Power (W)
OC
()
VUV + Neutral Treatment
VUV Treatment Only
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
1) Remove material
1) Add material
1) Change chemical or physical nature of the surface
Incident particle
Sputtered particle
Atomic collisions
Plasma surface treatment of polymers
Environmental advantages over conventional processes
bullEnhanced adhesion eg automotive - car bumpers
bullEnhanced wetability
bullSurface preparation for cell support
bullBio-compatibility - lens treatment
bullTextile treatment
bullMicro-electronics
PC
Mass SpectrometerProbe
HAL EQP 1000 Mass Spectrometer
Controller
Pirani gauge
Rotary Pump
Monomer vapour inlet
Substrate
OscilloscopePulse Generator
Matching Network
RF Generator and Power Meter
Turbo Pump
Glass chamber
LOW-PRESSURE PLASMA
Cell growth and viability on patterned surfaces
MG63 cells on plasma patterned PS after 48 hours culture The gaps width 200 m
5m
10m
AFM Friction image (O+N)C = 027
With RD Short ndash Sheffield
Controlling ion energy and flux
XPS analysis of the surface modification of polystyrene
0
3
6
9
12
15
18
0 5 10 15 20 25 30 35
Power (W)
OC
()
VUV + Neutral Treatment
VUV Treatment Only
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Plasma surface treatment of polymers
Environmental advantages over conventional processes
bullEnhanced adhesion eg automotive - car bumpers
bullEnhanced wetability
bullSurface preparation for cell support
bullBio-compatibility - lens treatment
bullTextile treatment
bullMicro-electronics
PC
Mass SpectrometerProbe
HAL EQP 1000 Mass Spectrometer
Controller
Pirani gauge
Rotary Pump
Monomer vapour inlet
Substrate
OscilloscopePulse Generator
Matching Network
RF Generator and Power Meter
Turbo Pump
Glass chamber
LOW-PRESSURE PLASMA
Cell growth and viability on patterned surfaces
MG63 cells on plasma patterned PS after 48 hours culture The gaps width 200 m
5m
10m
AFM Friction image (O+N)C = 027
With RD Short ndash Sheffield
Controlling ion energy and flux
XPS analysis of the surface modification of polystyrene
0
3
6
9
12
15
18
0 5 10 15 20 25 30 35
Power (W)
OC
()
VUV + Neutral Treatment
VUV Treatment Only
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
PC
Mass SpectrometerProbe
HAL EQP 1000 Mass Spectrometer
Controller
Pirani gauge
Rotary Pump
Monomer vapour inlet
Substrate
OscilloscopePulse Generator
Matching Network
RF Generator and Power Meter
Turbo Pump
Glass chamber
LOW-PRESSURE PLASMA
Cell growth and viability on patterned surfaces
MG63 cells on plasma patterned PS after 48 hours culture The gaps width 200 m
5m
10m
AFM Friction image (O+N)C = 027
With RD Short ndash Sheffield
Controlling ion energy and flux
XPS analysis of the surface modification of polystyrene
0
3
6
9
12
15
18
0 5 10 15 20 25 30 35
Power (W)
OC
()
VUV + Neutral Treatment
VUV Treatment Only
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Cell growth and viability on patterned surfaces
MG63 cells on plasma patterned PS after 48 hours culture The gaps width 200 m
5m
10m
AFM Friction image (O+N)C = 027
With RD Short ndash Sheffield
Controlling ion energy and flux
XPS analysis of the surface modification of polystyrene
0
3
6
9
12
15
18
0 5 10 15 20 25 30 35
Power (W)
OC
()
VUV + Neutral Treatment
VUV Treatment Only
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Controlling ion energy and flux
XPS analysis of the surface modification of polystyrene
0
3
6
9
12
15
18
0 5 10 15 20 25 30 35
Power (W)
OC
()
VUV + Neutral Treatment
VUV Treatment Only
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Pulsed plasma polymerisation
bull Wide range of potential applications
ndash Barrier coatings PET films to form packaging cartons
ndash Scratch resistant transparent coatings
ndash Anti-corrosive layers
ndash Anti-adhering anti-soiling coatings - ie baking trays pans etc
ndash Biocompatibility
Production of deposits by pulsed plasma polymerisation
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
CH2 CH CO
OH
bull Model for film growth
bull Time evolution of plasma parameters
bull XPS (with derivatisation) functional group quantification
Plasma polymerisation (Acrylic acid NIPAAm hexane allyl aminehellip)
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Plasmaa
Porous scaffold (varying lengths L)
ions neutrals radicals
Mass spec extraction electrode
L
Plasma deposition inside 3-D porous engineering scaffolds ndash Collaboration with M Alexander Nottingham
Cell (3T3 fibroblasts ) adhesive plasma deposits of allyl amine and cell repellent hexane coatings
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Using Knudsen diffusion in gaps
Laboratory of Biophysics and Surface Analysis School of Pharmacy The University of Nottingham University Park Nottingham NG7 2RD UK
Tissue Engineering Group School of Pharmacy Centre for Biomolecular Sciences The University of Nottingham University Park Nottingham NG7 2RD UK
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Water contact angle versus distance under the gap
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Average number of cells in 02 mm increments along the steep gradient (left ppHex right ppAAm) after 1() 2() and 3() days of incubation
The sample mask interface was set at the origin of the x-axis The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Study 3T3 fibroblasts cell interactions
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Average number of cells in 02 mm increments along the shallow gradient (left ppHex rightppAAm) after 1() 2() and 3() days of incubation
The columns to the right are the average cell number on the uniform ppAAm samples after 1 and 2 days
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Cell number on the shallow gradient after day 1() 2() and 3() plotted against the corresponding WCA The uniform samples (larger symbols) are shown for day 1 (ppHex 1048711ppAAm 1048711) and day 2 (ppHex 1048711 ppAAm 1048711) The error bars represent SEM (gradient n=15 uniform samples n=35)
Cell density as function of the surface energy - WCA
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Plasma physics- chemistry study - Acrylic acid
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
The orifice and end cap for detection of negative ions
Orifice at +65 V
End cap and spectrometer barrel at ground potential
Extracting ions from the plasma
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Surface Analysis
Functional group retention ndash by XPS
Pulsed plasma
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Time-averaged mass spectra for a pulse off-time of 10 ms
Series [nM+H]+ mz= 73 145 217
Series [nM-H]- mz = 71143215 287
Neutrals
Negative ions
Positive ions
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Negative ion mass spectra for pulse off times of 05 ms (a) and 10 ms (b)
Low masses detected
Higher masses detected
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Positive ion flux ndash time resolved
The time-resolved IEDF ion fluxes (a) and (b) - 48 sccm 50 W (c) and (d) - 15 sccm 50W Zero time point corresponds to the beginning of the on-pulse
217217 145
73
55
21755
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
287 amu
215
143
71
Negative ions ndash time resolved fluxes
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Negative ion structural assignments and potential production mechanisms
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
0
02
04
06
08
1
12
14
16
18
2
0 1000 2000 3000 4000 5000 6000 7000 8000 9000 10000
t (s)
nn
np (
10
15 m
-3)
np nn
Langmuir probe measurements of the negative and positive ion densities
Acrylic acid ndash pulsed RF 40ms Off time ndash 10 mTorr
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Atmospheric pressure plasmas
Uses Killing bacteria sterilize medical equipment food - decontaminate biological weapons deposition treatment polymerisation
M Laroussi Old Dominion University in Virginia
E Stoffels et al - TU Eindhoven
100k to 1M colony-forming units of E coli killed after 10 seconds Plasma powers lt 150 mW
Cold Plasma
ldquoPlasma NeedlerdquoNon-thermal atmospheric pressure plasmas
Uses Dentistry and Surgery
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Conclusions
Low-pressure plasma treatmentpolymerization is useful
Applications in many areas ndash Bio-surfaces flexible electronics etc
High-pressure and Atmospheric pressure plasma being developed
Activity in technological plasma research is relevant and timely
The synergy between plasma physics engineering chemistry surface science and bio-science will provide unique opportunities
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-
Micro-plasmas
Microplasma used for
1 UV radiation source ndash He Xe
2 Light sources- flat panel displays micro-lasers
3 Plasma-reactors
4 Surface modification ndash source of radical ands ions
5 Deposition - HMDSO
6 flow reactors maskless etching of Si
7 Analytical spectroscopy- liquid and gases
8 Photo detectors
J G Eden et al J Phys D Appl Phys 36 (2003)
University of IllinoisLaboratory for Optical Physics and Engineering
- Slide 1
- Slide 2
- Slide 3
- Slide 4
- Cell growth and viability on patterned surfaces
- Controlling ion energy and flux
- Slide 7
- Slide 8
- Slide 9
- Slide 10
- Slide 11
- Slide 12
- Slide 13
- Slide 14
- Slide 15
- Slide 16
- Slide 17
- Slide 18
- Slide 19
- Slide 20
- Slide 21
- Slide 22
- Slide 23
- Slide 24
- Conclusions
- Slide 26
-