Cassava Plants with a Depleted Cyanogenic Glucoside Content in Leaves and Tubers. Distribution of Cyanogenic

Glucosides, Their Site of Synthesis and Transport, and Blockage of the Biosynthesis by RNA Interference

Technology

by Kirsten Jørgensen, Søren Bak, Peter Kamp Busk, Charlotte Sørensen, Carl Erik Olsen, Johanna Puonti-Kaerlas, and Birger Lindberg Møller

PlantphysiolVolume 139(1):363-374

September 12, 2005

©2005 by American Society of Plant Biologists

The biosynthetic pathway for the cyanogenic glucosides linamarin and lotaustralin in cassava.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

LC-MS analysis of the content of linamarin in tubers (A) and the first unfolded leaf (B) in 6-month-old in vivo-grown cassava RNAi plants.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

The phenotype of transgenic RNAi plants in vitro.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

Cyanide potential of individual leaves, petioles, and stems of an entire in vivo-grown cassava plant.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

Stem girdling of a cassava plant.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

Variation in cyanide potential among individual plants belonging to the same cassava clone.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

Influence of nitrate and potassium fertilizer on cyanogenic glucoside content in cuttings from 6-week-old cassava plants as determined by LC-MS. sd is shown by error bars.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists

Cellular localization of CYP79D1and CYP79D2 in the first fully unfolded cassava leaf and the corresponding petiole determined by in tube in situ RT-PCR analysis using light microscopy of

100-μm transverse sections.

Jørgensen K et al. Plantphysiol 2005;139:363-374

©2005 by American Society of Plant Biologists