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    CHAPTER 6 IGCSE

    PLANT NUTRITION

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    THE EQUATION FORPHOTOSYNTHESIS

    Photosynthesisis the fundamenta !"#$ess %&!ants manufa$tu"e f##d m#e$ues (carbohydrf"#m "a' mate"ias(CO2and H2O) usin* energyf"#m light+

    The "a' mate"ias a"e Ci*ht ene"*&+

    The !"#du$ts a"e *u$#s

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    THE PROCESS OF PHOTOSYNTH

    G"een !ants ta.e in CO2th"#u*h thei" ea/es (%& difusion H2Ois a%s#"%ed th"#u*h !ants0 "##ts (%& osmosis)- and

    t"ans!#"ted t# the eaf th"#u*h xylem/esses+

    Chlorophyll t"a!s light energyand a%s#"%s it+

    This ene"*& is used t# %"ea. u! H,O m#e$ues- than t# %#nh&d"#*en and CO, t# f#"m glucose+

    Gu$#se is usua& $han*ed t# sucrose f#" t"ans!#"t a"#un!ant- #" t# starchf#" st#"a*e+

    O2is "eeased as a 'aste !"#du$t- #" used %& !ant f#" "es!

    In this !"#$ess- light energy is $#n/e"ted t# chemical enthe f#"mati#n #f *u$#se and its su%se1uent st#"a*e+

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    PHOTOSYNTHESIS IN2ESTIGATIOPRINCIPLE AN3 STARCH TEST

    E4!e"iments $an %e used t# 5nd #ut 'hat fa$t#"i*ht- $h##"#!h&) a"e needed f#" !h#t#s&nthesthe !ant need t# %e destarch+ T# %e $e"tain thaeafs a"e th#"#u*h& desta"$hed- testa eaff#" starch %ef#"e %e*in the in/esti*ati#n+

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    PRINCIPLE OF IN2ESTIGATION

    1.Inestigations need controls Control!ant (#" ea/e) has a su%stan$es it needs+

    !est!ant a$.s #ne su%stan$e (i*ht$h#"#!h&CO,)

    2. Plants must be destarched

    It is /e"& im!#"tant that the ea/es u a"e testin* sh#udan& starchin them at the %e*innin* #f the e4!e"iment+

    S#- 5"st #f a- u must desta"$h the !ants+ Lea/e them inthe dar"f#" 78 h#u"s+ The !ants use u! a st#"es #f sta"$ea/es+

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    #. $tarch test %ith Iodine solution

    Afte" a fe' h#u"s- $a""& #ut the sta"$h test #n %#th!ants9Iodine solutionis used: a %ue;%a$. $##u" #

    ea/e is !#siti/e+ &oil the lea' in %ater f#" %"ea. d#'n the mem%"ane > i#dine s#uti#n *eth"#u*h $e mem%"ane t# "ea$hstarchinside the $hand "ea$t 'ith them+

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    &oil the lea' in alcohol (ethan#) in a 'ate"%ath9The green colour#f the eaf and the %"#'i#dine s#uti#n $an ##. %a$. t#*ethe"- s# u n

    "em#/e chlorophyll%& dissolingit #ut 'ith aLea/e it unti a the $h#"#!h& has $#me #ut #f eaf+

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    (inse the lea' in %ater9 ?#iin* the eaf in ethma.es it %"itte- the 'ate" s#ftens it+

    S!"ead the eaf #ut #n a %hitetie > eas& t# see

    "esut+ dd iodine solutiont# the eaf > %ue; %a$. $#

    !#siti/e- sta"$h is !"esent+

    A leaf before (on the left) and after (on the right) starch test

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    PHOTOSYNTHESIS IN2ESTIGATIO(CH*O(OPH+**, CO2 AN3 *I-

    TESTS)

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    CHLOROPHYLL

    ProcessTa.e a !#tted !ant 'ith ariegated (*"een and

    ea/es+

    estarch the !ant %& .ee!in* it in $#m!ete daf#" a%#ut 78 h#u"s+

    E4!#se the !ant t# the sunlightf#" a fe' da&sTest #ne #f the ea/es f#" starch 'ith i#dine s#

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    Obserations; A"eas 'ith !"e/i#us& green!at$hes test positi%ue %a$.)+

    ; A"eas 'ith !"e/i#us& !ae yello% !at$hestest negatie ("emain %"#'n)+ Conclusion

    - Photosynthesis takes plapatches because of the preof chlorophyll.

    - The pale yellow patches d

    photosynthesis because of chlorophyll.

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    *I-H!ProcessTa.e a !#tted !ant+

    estarch the !ant %& .ee!in* it in $#m!ete da"a%#ut 78 h#u"s+Test #ne #f it ea/es f#" sta"$h- t# $he$. that is d#$#ntain an&+Fi4 a eaf #f this !ant in %et'een t'# st"i!s #f a t

    #n eaf+Pa$e the !ant in i*ht f#" a fe' da&s+Rem#/e the $#/e" f"#m the eaf and test it f#" sta

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    ObserationsP#siti/e sta"$h test 'i %e #%tained #n& in the !#"the eaf e4!#sed t# i*ht and ne*ati/e test in !a"ts!a!e" st"i!+

    Conclusion

    Light is necessary fophotosynthesis.

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    C(&O/ IO0IProcessTa.e t'# desta"$hed !#tted !ants+C#/e" %#th the !ants 'ith %e @a"s and a%e them

    and ?+Inside Set;u! A- .ee! /aHCO#(s#dium %i$a"%#na

    !"#du$es CO,+

    Inside Set;u! ?- .ee! /aOH(S#dium h&d"#4ide)+

    a%s#"%s CO,+ee! %#th the set;u!s in the suni*ht at east f#" 6h#u"s+Pe"f#"m the sta"$h test #n %#th #f the !ants+

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    Obserations

    Leaf f"#m the !ant in 'hi$h /aHCO#has %een !a$ed*i/es positietest+Leaf f"#m the !ant in 'hi$h /aOH has %een .e!t*i/e negati' test+ConclusionPant in Set u! A *ets CO

    ,'he"eas !ant in Set;u! ? d#e

    n#t *et CO,+

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    C! O *I-H! I/!/$I!+ O/ !H (! OPHO!O$+/!H$I$

    Pants need i*ht ene"*&

    t# ma.e the $hemi$aene"*& needed t# $"eate$a"%#h&d"ates+

    In$"easin* the i*htintensit& 'i %##st thes!eed #f !h#t#s&nthesis+

    H#'e/e"- at hi*h i*htintensities the "ate%e$#mes $#nstant+

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    xplanation

    *ight energy absorbed by chlorophyll is conerted to

    At /e"& #' i*ht e/es the !ant 'i %e "es!i"in* #n& n#t!h#t#s&nthesiBin*+

    As the light intensity increases, the rate o' !h#t#s&ntheincreases.

    Ho%eer, the rate %ill not increase beyond a $e"tain e/intensit&+

    At hi*h i*ht intensities the "ate %e$#mes constant, een %in$"eases in i*ht intensit&- the"e a"e n# in$"eases in the "ate

    The !ant is una%e t# ha"/est the i*ht at these hi*h intensit$h#"#!h& s&stem $an %e dama*ed %& /e"& intense i*ht e/

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    EFFECT OF !4P(!5(ON THE RATE OFPHOTOSYNTHESIS

    hen the temperaturerises the rate o'photosynthesis risesalso. !here is

    an optimumtemperature at %hichthe rate o'photosynthesis ismaximum.

    ?end this tem!e"atu"e-the "ea$ti#n 1ui$.&$#mes t# a hat+

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    xplanation

    ! *O6 !4P(!5(- the enB&me does not have enouenergyt# meet man& su%st"ate m#e$ues- s# the "ea$ti#n

    hen the tem!e"atu"e "ises- the !a"ti$es in the "ea$ti#n moquicker and collide more- s# the "ate #f !h#t#s&nthesis "is

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    ! !H OP!I454 !4P(!5(- the enB&meefcient and the "ate if ma4imum+

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    t !4P(!5($ &O7 89:C the rate slo%do%n.

    This is %e$ause the enB&mes in/#/ed in the $he"ea$ti#ns #f !h#t#s&nthesis a"e tem!e"atu"e sen

    and destroyed (denatured) at higher

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    fect o' Carbon Dioxide on the (ate o'Photosynthesis

    hen the $#n$ent"ati#n #fCO, is #' the "ate #f!h#t#s&nthesis is as# #'+(the !ant has t# s!end time'aitin* f#" m#"e CO, t#

    a""i/e)+ In$"easin* the $#n$ent"ati#n#f CO in$"eases the "ate #f!h#t#s&nthesis+

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    xplanation

    The "ate #f !h#t#s&nthesis

    increases linearly %ithin$"easin* CO,$#n$ent"ati#n (f"#m !#int At# ? )+

    The "ate fas *"adua&-

    and at a $e"tain CO,$#n$ent"ati#n it sta&s$#nstant (f"#m !#int ? t#C)+

    He"e a "ise in CO, e/es

    has no efect as the

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    Limitin* fa$t#"s in !h#t#s&nthes

    *imiting 'actoris s#methin* !"esent in the

    en/i"#nment in su$h short supply that it "est"i$t!"#$esses+

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    $unlight

    O/e" the 5"st !a"t #f the $u"/e(between A and )- i*ht is alimiting 'actor+ The !ant isimited in h#' fast it $an!h#t#s&nthesise %e$ause it

    d#es n#t ha/e en#u*h i*ht+ etween and C- i*ht is n#t

    a imitin* fa$t#"+ E/en if m#"ei*ht is sh#ne #n the !ant- itsti $ann#t !h#t#s&nthesise

    an& faste"+

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    Carbon dioxide

    In !h#t#s&nthesis CO,is a .e&imitin* fa$t#"+ The usuaatm#s!he"i$ e/e #f CO,is =+=

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    Tem!e"atu"e

    The $hemi$a "ea$ti#ns #f !h#ts&nthe$an #n& ta.e !a$e /e"& s#'& at #'tem!e"atu"e- s# a !ant $an!h#t#s&nthesise faste" #n a 'a"m dan a $#d #ne+

    i di i f h h i

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    O!timum $#nditi#ns f#" !h#t#s&nthesis G"een h#use

    if $"#!s a"e *"#'n in*assh#uses- it is !#ssi%et# $#nt"# $#nditi#ns s# thatthe& a"e !h#t#s&nthesisin*as fast as !#ssi%e+

    CO i h t

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    CO2enrichment

    In a $#sed *assh#use- it is !#ssi%e t# !"#/ide CO,f#" the !ants- e+*+ %& %u"nin* f#ssi fues #""eeasin* !u"e CO,f"#m a *as $&inde"+

    Optimum light

    In $#ud& #" da". $#nditi#ns- e4t"a a"ti5$ia i*ht%e !"#/ided- s# that i*ht is n#t imitin* the "ate !h#t#s&nthesis+

    Optimum temperature

    The tem!e"atu"e $an %e "aised %& usin* a heatis&stem+ If f#ssi fues a"e %u"ned- the"e is as# a f"#m the CO2!"#du$ed+

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    Leaf st"u$tu"e

    The eaf $#nsist #f a%"#ad- at !a"t $aedthe lamina- 'hi$h is

    @#ined t# the "est #f the!ant %& a eaf sta.#" petiole+ Runnin*th"#u*h the !eti#e

    a"e ascular bundles-'hi$h then f#"mthe einsin the eaf+

    Ath#u*h a eaf ##.s thin its is made u! #f see

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    Ath#u*h a eaf ##.s thin- its is made u! #f seelayers#f $es+ Y#u $an see these if u ##. at at"ans/e"se se$ti#n ($"#ss;se$ti#n) #f a eaf unde"mi$"#s$#!e+

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    !"omework

    3"a' $"#ss se$ti#n #f eaf 'ith a%La%e 'ith fun$ti#n (use #C$)/ame- Cha"a$te"isti$- un$ti#n+

    E4!ain %"ie& theADA%&A&'#s#finte"na st"u$tu"e #f eaf f#" !h#t#s&n

    E4am!e

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    E4am!e

    5PP( PI(4I$ thin and transparent ;

    allo%s light to passthrough

    no chloroplasts arepresent

    act as a barrier todisease organisms

    Cuticle

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    Lower

    epiderm

    Upper

    epiderm

    Cuticle

    Palisade

    mesophyll

    Vascular

    bundle

    Xylem

    Phloem

    Spong

    mesoph

    Airspace

    stoma

    CUTICLE

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    CUTICLE

    eatures !

    characteristics Co"ersthe

    epidermis

    A transparent layer

    o# cutin $a%yco"ering&

    waterproo#

    unction

    '( Protectthe lea#)( Allow lightto enter the lea#

    *( Pre"ente%cessi"e water loss

    UPPE+ EPI,E+-IS

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    UPPE+ EPI,E+-IS

    eatures !

    characteristics A single layero#

    #lattenedcells

    ,oes notcontain

    chloroplast Transparent

    unction

    '( Allow lightto penetrate the lea# andthe mesophyll layer

    PALISA,E -ES.P/0LL

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    PALISA,E -ES.P/0LL

    eatures !

    characteristics .ne or #ew rows o#

    "erticallyorientedclosely pac1edcolumn2shapedcells

    /a"e a highdensity o#chloroplast

    unction

    '( Trap most o# the incoming light

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    SP.340 -ES.P/0L

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    SP.340 -ES.P/0L

    eatures !

    characteristics Loosely pac1ed

    irregularly shapedcells

    /a"e#ewerchloroplast

    unction

    '( Increase the internal sur#acearea #gaseous e%change

    )( Trape%cessi"e light #rom palisademesophyll

    VASCULA+ 5U3,LE

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    VASCULA+ 5U3,LE

    eatures !

    characteristics Consists o#%ylem

    and phloem

    unction

    '( Xylem 6 transports mineral ions a

    waterto the lea#)( Phloem2 transport products o#

    photosynthesisaway #rom the lea#

    AI+ SPACE

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    AI+ SPACE

    eatures !

    characteristics Spaces between

    spongy mesophyll

    unction

    '( Allowdi##usion o# gases betweentatmosphere and the mesophyll(

    L.$E+ EPI,E+-IS

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    L.$E+ EPI,E+-IS

    eatures !

    characteristics Layer o# epidermal

    cells

    ,oes not contain

    chloroplast e%cept#or the guard cells

    unction

    '( Protectthe lea#)( Allow gaseous e%change through

    stomata

    4UA+, CELLS !ST.-A

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    4UA+, CELLS !ST.-A

    eatures !

    characteristics Thinner outerwalls

    Thic1er innerwalls

    -odi#iedlower

    epidermis

    7 Each stomata is

    #lan1ed by two guard

    cells which regulate

    the si8e o# the pores

    unction'( +egulatethe

    opening and clos

    o# the stomatal po

    Pants mine"a "e1ui"ements an

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    fe"tiiBe"s Pant is in need f#" mineral ions t# $#nt"# $hem

    a$ti/ities- *"#'- and !"#du$e mate"ias+ The m#s

    im!#"tant mine"as a"e 4agnesiumi#ns and /

    +Im!#"tan$e #f nit"ate andi i

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    ma*nesium i#ns

    . /itrates

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    . /itrates

    plants absorb nitrate ions from the

    soil, through their root hairs nitrate ions combine with glucose

    ----- amino acids

    amino acids bond together----- protein

    deficiencycauses poor growth,especially of lea!es. The stembecomes weak, lower lea!es becomeyellow and die, while upper lea!esturn pale green

    % a*nesium

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    %+a*nesium

    plants absorb magnesium ions

    from the soil solution used for the manufacture

    of chlorophyll

    each chlorophyll contains onemagnesium atom

    deficiencymakes lea!es turnyellow from the bottom of the stem

    upwards and e!entually stopsphotosynthesis