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Pilar Ruiz-Lozano, PhD CV, October 2018

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PILAR RUIZ-LOZANO, PhD

Chief Scientific Officer

EDUCATION July 1987 B.Sc. (Major Biology). University of Barcelona July 1988 M.Sc. Biochemistry. University of Barcelona. Nov. 1992 Ph.D. Biochemistry and Molecular Biology. University of Barcelona.

EMPLOYMENT 1989-1992 Teaching Assistant, University of Barcelona 1993-1998 Post-Graduate Researcher. Department of Medicine. School of Medicine. University of California, San Diego. Supervisor: Dr. Kenneth R. Chien 1998-2003 Assistant Project Molecular Biologist. UC San Diego 2003-2005 Assistant Research Scientist. UC San Diego 2005-2010 Assistant Professor, Sanford-Burnham-Prebys Medical Research Institute 2010-2016 Associate Professor, Stanford University 2015-2017 Stanford University, Leave of Absence 2015-date Chief Scientific Officer, RegenCor Inc

ENTREPRENEURSHIP 2015 Founder REGENCOR Inc., a Delaware Corporation

PATENTS AND INVENTIONS “EPICARDIAL-DERIVED PARACRINE FACTORS FOR REPAIRING MYOCARDIAL TISSUE” Inventors: Pilar Ruiz-Lozano, et al. Application No: 62196766. Filed July 24, 2015 “COMPOSITIONS AND METHODS FOR TREATING CARDIOVASCULAR AND PULMONARY DISEASES AND DISORDERS WITH APELIN” Inventors: Jayakumar Rajadas, et al. Filing number US 62/080,025 “ENGINEERED COLLAGEN MATRICES FOR MYOCARDIAL THERAPY” Inventors: Pilar Ruiz-Lozano, et al. Serial No.: 61/718,046; Filed: October 24, 2012 Stanford Ref. No.: S11-458; Sanford-Burnham Ref. No.: 13-002-01PRPV No.: 3815.109 “NOVEL GPCR AND METHODS OF USE OF THE SAME” Inventors: Pilar Ruiz-Lozano Publication number US20090010909 A1 Publication date Jan 8, 2009 Filing date May 7, 2008

Pilar Ruiz-Lozano, PhD

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SERVICE Ad-hoc Reviewer: At least twice a year I perform regular service as an ad-hoc reviewer in the following study sections: o NIH, Cardiovascular Differentiation and Development Study Section o NIH, Special Emphasis Panel Cardiac Development o NIH, Cardiac Hypertrophy Study Section o NIH, Special Emphasis Panel, Heart Failure o NIH, SBIR

Permanent Member: American Heart Association Review Panel, (2007-2009) American Heart Association Review Panel (current)

Current Ad-hoc Reviewer: NIH SBRI Study Section(s), Teleton (Italy), Agència Catalana de Reçerca (Catalan Government, Spain), ANEP (Spanish Government), among others. Ad-hoc Editor: Circulation Journal Reviewer: o Proc. Natl. Acad. Sci. USA o Development o Circulation o Developmental Biology z o Developmental Dynamics o Circulation Research o Nature Communications

PEER-REVIEWED PUBLICATIONS (1-64)

68 publications; 10,252 citations; h-index 39; i10-index 58 (data from Google Scholar, October 2018)

Parikh V, Liu J, Shang C, Woods C, Chang A, Zhao M, Charo D, Grunwald Z, Huang Y, Seo K, Tsao P, Bernstein D, Ruiz-Lozano P, Quertermous T, and Ashley E. Apelin and APJ orchestrate complex tissue-specific control of cardiomyocyte hypertrophy and contractility in the hypertrophy-heart failure transition. Am. J. Physiology 2018. doi: 10.1152/ajpheart.00693.2017. PubMed PMID: 29775410. Diez-Cuñado, M, Wei K, Bushway P, Maurya MR, Perera, R, Subramaniam S, Ruiz-Lozano , P*, Mark Mercola*. miRNAs that induce human cardiomyocyte proliferation converge on the Hippo pathway. Cell Reports 2018;23(7):2168-74. doi: 10.1016/j.celrep.2018.04.049. PubMed PMID: 29768213. Cunningham TJ, Yu MS, McKeithan WL, Spiering S, Carrette F, Huang CT, Bushway PJ, Tierney M, Albini S, Giacca M, Mano M, Puri PL, Sacco A, Ruiz-Lozano P, Riou JF, Umbhauer M, Duester G, Mercola M, Colas AR. Id genes are essential for early heart formation. Genes Dev. 2017. doi: 10.1101/gad.300400.117. PubMed PMID: 28794185. Eschenhagen, T, Bolli, R, Braun, T, Field, LJ, Fleischmann, BK, Frisén, J, Giacca, M,. Hare, JM, Houser, SR, Lee, RT, Marbán, E,. Martin, JF, Molkentin, JD, Murry, CE, Riley, PR, Ruiz-Lozano, P, Sadek, HA, Sussman, MA, Hill, JA. Cardiomyocyte Regeneration: A Consensus Statement Circulation 2017; 136:00–00. DOI: 10.1161/CIRCULATIONAHA.117.029343


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Crespo R, Wei K, Rodenak-Kladniew B, Mercola M, Ruiz-Lozano P, Hurtado C. Effect of geraniol on rat cardiomyocytes and its potential use as a cardioprotective natural compound. Life Sci. 2017;172:8-12. doi: 10.1016/j.lfs.2017.01.008. PubMed PMID: 28131760.

Mahmoudi M, Zhao M, Matsuura Y, Laurent S, Yang PC, Bernstein D, Ruiz-Lozano P, Serpooshan V. Infection-resistant MRI-visible scaffolds for tissue engineering applications. Bioimpacts. 2016;6(2):111-5. doi: 10.15171/bi.2016.16. PubMed PMID: 27525229; PMCID: PMC4981249.

Diaz-Trelles R, Scimia MC, Bushway P, Tran D, Monosov A, Monosov E, Peterson K, Rentschler S, Cabrales P, Ruiz-Lozano P, Mercola M. Notch-independent RBPJ controls angiogenesis in the adult heart. Nature Commun. 2016;7:12088. doi: 10.1038/ncomms12088. PubMed PMID: 27357444; PMCID: PMC4931341.

Wei K, Serpooshan V, Hurtado C, Diez-Cunado M, Zhao M, Maruyama S, Zhu W, Fajardo G, Noseda M, Nakamura K, Tian X, Liu Q, Wang A, Matsuura Y, Bushway P, Cai W, Savchenko A, Mahmoudi M, Schneider MD, van den Hoff MJ, Butte MJ, Yang PC, Walsh K, Zhou B, Bernstein D, Mercola M, Ruiz-Lozano P. Epicardial FSTL1 reconstitution regenerates the adult mammalian heart. Nature. 2015;525(7570):479-85. doi: 10.1038/nature15372. PubMed PMID: 26375005; PMCID: PMC4762253. Full Article. Faculty1000Prime Article

Wei K, Diaz-Trelles R, Liu Q, Diez-Cunado M, Scimia MC, Cai W, Sawada J, Komatsu M, Boyle JJ, Zhou B, Ruiz-Lozano P, Mercola M. Developmental origin of age-related coronary artery disease. Cardiovasc Res. 2015;107(2):287-94. doi: 10.1093/cvr/cvv167. PubMed PMID: 26054850; PMCID: PMC4565989.

Serpooshan V, Sivanesan S, Huang X, Mahmoudi M, Malkovskiy AV, Zhao M, Inayathullah M, Wagh D, Zhang XJ, Metzler S, Bernstein D, Wu JC, Ruiz-Lozano P, Rajadas J. [Pyr1]-Apelin-13 delivery via nano-liposomal encapsulation attenuates pressure overload-induced cardiac dysfunction. Biomaterials. 2015;37:289-98. doi: 10.1016/j.biomaterials.2014.08.045. PubMed PMID: 25443792.

Serpooshan V, Mahmoudi M, Zhao M, Wei K, Sivanesan S, Motamedchaboki K, Malkovskiy AV, Gladstone AB, Cohen JE, Yang PC, Rajadas J, Bernstein D, Woo YJ, Ruiz-Lozano P. Protein Corona Influences Cell-Biomaterial Interactions in Nanostructured Tissue Engineering Scaffolds. Adv Funct Mater. 2015;25(28):4379-89. doi: 10.1002/adfm.201500875. PubMed PMID: 27516731; PMCID: PMC4978190.

Serpooshan V, Zhao M, Metzler SA, Wei K, Shah PB, Wang A, Mahmoudi M, Malkovskiy AV, Rajadas J, Butte MJ, Bernstein D, Ruiz-Lozano P. Use of bio-mimetic three-dimensional technology in therapeutics for heart disease. Bioengineered. 2014;5(3):193-7. doi: 10.4161/bioe.27751. PubMed PMID: 24637710; PMCID: PMC4101012.


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Serpooshan V, Ruiz-Lozano P. Ultra-rapid manufacturing of engineered epicardial substitute to regenerate cardiac tissue following acute ischemic injury. Methods in molecular biology. 2014;1210:239-48. doi: 10.1007/978-1-4939-1435-7_18. PubMed PMID: 25173173.

Serpooshan V, Zhao M, Metzler SA, Wei K, Shah PB, Wang A, Mahmoudi M, Malkovskiy AV, Rajadas J, Butte MJ, Bernstein D, Ruiz-Lozano P. The effect of bioengineered acellular collagen patch on cardiac remodeling and ventricular function post myocardial infarction. Biomaterials. 2013;34(36):9048-55. doi: 10.1016/j.biomaterials.2013.08.017. PubMed PMID: 23992980; PMCID: PMC3809823.

Nam J, Onitsuka I, Hatch J, Uchida Y, Ray S, Huang S, Li W, Zang H, Ruiz-Lozano P, Mukouyama YS. Coronary veins determine the pattern of sympathetic innervation in the developing heart. Development. 2013;140(7):1475-85. doi: 10.1242/dev.087601. PubMed PMID: 23462468; PMCID: PMC3596991.

Mahmoudi M, Abdelmonem AM, Behzadi S, Clement JH, Dutz S, Ejtehadi MR, Hartmann R, Kantner K, Linne U, Maffre P, Metzler S, Moghadam MK, Pfeiffer C, Rezaei M, Ruiz-Lozano P, Serpooshan V, Shokrgozar MA, Nienhaus GU, Parak WJ. Temperature: the "ignored" factor at the NanoBio interface. ACS nano. 2013;7(8):6555-62. doi: 10.1021/nn305337c. PubMed PMID: 23808533.

Thomas PS, Sridurongrit S, Ruiz-Lozano P, Kaartinen V. Deficient signaling via Alk2 (Acvr1) leads to bicuspid aortic valve development. PLoS One. 2012;7(4):e35539. doi: 10.1371/journal.pone.0035539. PubMed PMID: 22536403; PMCID: PMC3334911.

Scimia MC, Hurtado C, Ray S, Metzler S, Wei K, Wang J, Woods CE, Purcell NH, Catalucci D, Akasaka T, Bueno OF, Vlasuk GP, Kaliman P, Bodmer R, Smith LH, Ashley E, Mercola M, Brown JH, Ruiz-Lozano P. APJ acts as a dual receptor in cardiac hypertrophy. Nature. 2012;488(7411):394-8. doi: 10.1038/nature11263. PubMed PMID: 22810587; PMCID: PMC3422434. Faculty1000 article

Llagostera E, Alvarez Lopez MJ, Scimia C, Catalucci D, Parrizas M, Ruiz-Lozano P, Kaliman P. Altered beta-adrenergic response in mice lacking myotonic dystrophy protein kinase. Muscle Nerve. 2012;45(1):128-30. doi: 10.1002/mus.22256. PubMed PMID: 22190319; PMCID: PMC3422658.

Prigozhina NL, Heisel A, Wei K, Noberini R, Hunter EA, Calzolari D, Seldeen JR, Pasquale EB, Ruiz-Lozano P, Mercola M, Price JH. Characterization of a novel angiogenic model based on stable, fluorescently labelled endothelial cell lines amenable to scale-up for high content screening. Biology of the Cell / under the auspices of the European Cell Biology Organization. 2011;103(10):467-81. doi: 10.1042/BC20100146. PubMed PMID: 21732911; PMCID: PMC3538155.

Mercola M, Ruiz-Lozano P, Schneider MD. Cardiac muscle regeneration: lessons from development. Genes & Development. 2011;25(4):299-309. doi: 10.1101/gad.2018411. PubMed PMID: 21325131; PMCID: PMC3042154.


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Brade T, Kumar S, Cunningham TJ, Chatzi C, Zhao X, Cavallero S, Li P, Sucov HM, Ruiz-Lozano P, Duester G. Retinoic acid stimulates myocardial expansion by induction of hepatic erythropoietin which activates epicardial Igf2. Development. 2011;138(1):139-48. doi: 10.1242/dev.054239. PubMed PMID: 21138976; PMCID: PMC2998168.

Iqbal Z, Cejudo-Martin P, de Brouwer A, van der Zwaag B, Ruiz-Lozano P, Scimia MC, Lindsey JD, Weinreb R, Albrecht B, Megarbane A, Alanay Y, Ben-Neriah Z, Amenduni M, Artuso R, Veltman JA, van Beusekom E, Oudakker A, Millan JL, Hennekam R, Hamel B, Courtneidge SA, van Bokhoven H. Disruption of the podosome adaptor protein TKS4 (SH3PXD2B) causes the skeletal dysplasia, eye, and cardiac abnormalities of Frank-Ter Haar Syndrome. Am J Hum Genet. 2010;86(2):254-61. doi: 10.1016/j.ajhg.2010.01.009. PubMed PMID: 20137777; PMCID: PMC2820172.

Denzel MS, Scimia MC, Zumstein PM, Walsh K, Ruiz-Lozano P, Ranscht B. T-cadherin is critical for adiponectin-mediated cardioprotection in mice. J Clin Invest. 2010;120(12):4342-52. doi: 10.1172/JCI43464. PubMed PMID: 21041950; PMCID: PMC2993592.

Fink M, Callol-Massot C, Chu A, Ruiz-Lozano P, Izpisua Belmonte JC, Giles W, Bodmer R, Ocorr K. A new method for detection and quantification of heartbeat parameters in Drosophila, zebrafish, and embryonic mouse hearts. Biotechniques. 2009;46(2):101-13. doi: 10.2144/000113078. PubMed PMID: 19317655; PMCID: PMC2855226.

Buechling T, Akasaka T, Vogler G, Ruiz-Lozano P, Ocorr K, Bodmer R. Non-autonomous modulation of heart rhythm, contractility and morphology in adult fruit flies. Dev Biol. 2009;328(2):483-92. doi: 10.1016/j.ydbio.2009.02.013. PubMed PMID: 19233157; PMCID: PMC2829972.

Sridurongrit S, Larsson J, Schwartz R, Ruiz-Lozano P, Kaartinen V. Signaling via the Tgf-beta type I receptor Alk5 in heart development. Dev Biol. 2008;322(1):208-18. doi: 10.1016/j.ydbio.2008.07.038. PubMed PMID: 18718461; PMCID: PMC2677203.

Huang X, Gao X, Diaz-Trelles R, Ruiz-Lozano P, Wang Z. Coronary development is regulated by ATP-dependent SWI/SNF chromatin remodeling component BAF180. Dev Biol. 2008;319(2):258-66. doi: 10.1016/j.ydbio.2008.04.020. PubMed PMID: 18508041.

Zamora M, Manner J, Ruiz-Lozano P. Epicardium-derived progenitor cells require beta-catenin for coronary artery formation. Proc Natl Acad Sci U S A. 2007;104(46):18109-14. doi: 10.1073/pnas.0702415104. PubMed PMID: 17989236; PMCID: PMC2084304.

Ruiz-Lozano P, Rajan P. Stem cells as in vitro models of disease. Curr Stem Cell Res Ther. 2007;2(4):280-92. PubMed PMID: 18220912.

Llagostera E, Catalucci D, Marti L, Liesa M, Camps M, Ciaraldi TP, Kondo R, Reddy S, Dillmann WH, Palacin M, Zorzano A, Ruiz-Lozano P, Gomis R, Kaliman P. Role of myotonic dystrophy protein kinase (DMPK) in glucose homeostasis and muscle insulin action. PLoS One.


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2007;2(11):e1134. doi: 10.1371/journal.pone.0001134. PubMed PMID: 17987120; PMCID: PMC2043489.

Franco D, Moreno N, Ruiz-Lozano P. Non-resident stem cell populations in regenerative cardiac medicine. Cell Mol Life Sci. 2007;64(6):683-91. doi: 10.1007/s00018-007-6521-4. PubMed PMID: 17380309.

Care A, Catalucci D, Felicetti F, Bonci D, Addario A, Gallo P, Bang ML, Segnalini P, Gu Y, Dalton ND, Elia L, Latronico MV, Hoydal M, Autore C, Russo MA, Dorn GW, 2nd, Ellingsen O, Ruiz-Lozano P, Peterson KL, Croce CM, Peschle C, Condorelli G. MicroRNA-133 controls cardiac hypertrophy. Nature Med. 2007;13(5):613-8. doi: 10.1038/nm1582. PubMed PMID: 17468766.

Wang J, Hoshijima M, Lam J, Zhou Z, Jokiel A, Dalton ND, Hultenby K, Ruiz-Lozano P, Ross J, Jr., Tryggvason K, Chien KR. Cardiomyopathy associated with microcirculation dysfunction in laminin alpha4 chain-deficient mice. J Biol Chem. 2006;281(1):213-20. doi: 10.1074/jbc.M505061200. PubMed PMID: 16204254.

St Amand TR, Lu JT, Zamora M, Gu Y, Stricker J, Hoshijima M, Epstein JA, Ross JJ, Jr., Ruiz-Lozano P*, Chien KR*. Distinct roles of HF-1b/Sp4 in ventricular and neural crest cells lineages affect cardiac conduction system development. Dev Biol. 2006;291(2):208-17. doi: 10.1016/j.ydbio.2005.10.018. PubMed PMID: 16430881.

Merki E, Zamora M, Raya A, Kawakami Y, Wang J, Zhang X, Burch J, Kubalak SW, Kaliman P, Izpisua Belmonte JC, Chien KR, Ruiz-Lozano P. Epicardial retinoid X receptor alpha is required for myocardial growth and coronary artery formation. Proc Natl Acad Sci U S A. 2005;102(51):18455-60. doi: 10.1073/pnas.0504343102. PubMed PMID: 16352730; PMCID: PMC1317903.

Martinez-Lopez MJ, Alcantara S, Mascaro C, Perez-Branguli F, Ruiz-Lozano P, Maes T, Soriano E, Buesa C. Mouse neuron navigator 1, a novel microtubule-associated protein involved in neuronal migration. Mol Cell Neurosci. 2005;28(4):599-612. doi: 10.1016/j.mcn.2004.09.016. PubMed PMID: 15797708.

Kaliman P, Catalucci D, Lam JT, Kondo R, Gutierrez JC, Reddy S, Palacin M, Zorzano A, Chien KR, Ruiz-Lozano P. Myotonic dystrophy protein kinase phosphorylates phospholamban and regulates calcium uptake in cardiomyocyte sarcoplasmic reticulum. J Biol Chem. 2005;280(9):8016-21. doi: 10.1074/jbc.M412845200. PubMed PMID: 15598648.

Fujioka M, Wessells RJ, Han Z, Liu J, Fitzgerald K, Yusibova GL, Zamora M, Ruiz-Lozano P, Bodmer R, Jaynes JB. Embryonic even skipped-dependent muscle and heart cell fates are required for normal adult activity, heart function, and lifespan. Circulation Res. 2005;97(11):1108-14. doi: 10.1161/01.RES.0000191546.08532.B2. PubMed PMID: 16239588; PMCID: PMC2726805.


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Andrade J, Lam JT, Zamora M, Huang C, Franco D, Sevilla N, Gruber PJ, Lu JT, Ruiz-Lozano P. Predominant fusion of bone marrow-derived cardiomyocytes. Cardiovascular Res. 2005;68(3):387-93. doi: 10.1016/j.cardiores.2005.09.016. PubMed PMID: 16256964.

Ruiz-Lozano P, Chien KR. Cre-constructing the heart. Nature Genet. 2003;33(1):8-9. doi: 10.1038/ng0103-8. PubMed PMID: 12509773.

Minamisawa S, Hiratsuka E, Ruiz-Lozano P, Machida S, Furutani Y, Nishimura M, Takao A, Yanagisawa K, Momma K, Saeki Y, Matsuoka R. Ectopic expression of an embryonic skeletal myosin heavy chain in human fetal and Syrian hamster hearts. J Muscle Res Cell Motil. 2003;24(7):399-406. PubMed PMID: 14677642.

Kioussi C, Briata P, Baek SH, Rose DW, Hamblet NS, Herman T, Ohgi KA, Lin C, Gleiberman A, Wang J, Brault V, Ruiz-Lozano P, Nguyen HD, Kemler R, Glass CK, Wynshaw-Boris A, Rosenfeld MG. Identification of a Wnt/Dvl/beta-Catenin --> Pitx2 pathway mediating cell-type-specific proliferation during development. Cell. 2002;111(5):673-85. PubMed PMID: 12464179.

Hamblet NS, Lijam N, Ruiz-Lozano P, Wang J, Yang Y, Luo Z, Mei L, Chien KR, Sussman DJ, Wynshaw-Boris A. Dishevelled 2 is essential for cardiac outflow tract development, somite segmentation and neural tube closure. Development. 2002;129(24):5827-38. PubMed PMID: 12421720.

Ferrer-Martinez A, Ruiz-Lozano P, Chien KR. Mouse PeP: a novel peroxisomal protein linked to myoblast differentiation and development. Dev Dyn. 2002;224(2):154-67. doi: 10.1002/dvdy.10099. PubMed PMID: 12112469.

Ruiz-Lozano P, Nakamura T, Chien KR. [Developmental genes and heart disease]. Rev Esp Cardiol. 2001;54(12):1439-45. PubMed PMID: 11754790.

Giordano FJ, Gerber HP, Williams SP, VanBruggen N, Bunting S, Ruiz-Lozano P, Gu Y, Nath AK, Huang Y, Hickey R, Dalton N, Peterson KL, Ross J, Jr., Chien KR, Ferrara N. A cardiac myocyte vascular endothelial growth factor paracrine pathway is required to maintain cardiac function. Proc Natl Acad Sci U S A. 2001;98(10):5780-5. doi: 10.1073/pnas.091415198. PubMed PMID: 11331753; PMCID: PMC33290.

Canicio J, Ruiz-Lozano P, Carrasco M, Palacin M, Chien K, Zorzano A, Kaliman P. Nuclear factor kappa B-inducing kinase and Ikappa B kinase-alpha signal skeletal muscle cell differentiation. J Biol Chem. 2001;276(23):20228-33. doi: 10.1074/jbc.M100718200. PubMed PMID: 11279241.

Ruiz-Lozano P, Ryan AK, Izpisua-Belmonte JC. Left-right determination. Trends Cardiovasc Med. 2000;10(6):258-62. PubMed PMID: 11282304.


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Nguyen-Tran VT, Kubalak SW, Minamisawa S, Fiset C, Wollert KC, Brown AB, Ruiz-Lozano P, Barrere-Lemaire S, Kondo R, Norman LW, Gourdie RG, Rahme MM, Feld GK, Clark RB, Giles WR, Chien KR. A novel genetic pathway for sudden cardiac death via defects in the transition between ventricular and conduction system cell lineages. Cell. 2000;102(5):671-82. PubMed PMID: 11007485.

Chu PH, Ruiz-Lozano P, Zhou Q, Cai C, Chen J. Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system. Mech Dev. 2000;95(1-2):259-65. PubMed PMID: 10906474.

Zhou Q, Ruiz-Lozano P, Martone ME, Chen J. Cypher, a striated muscle-restricted PDZ and LIM domain-containing protein, binds to alpha-actinin-2 and protein kinase C. J Biol Chem. 1999;274(28):19807-13. PubMed PMID: 10391924.

Tsukui T, Capdevila J, Tamura K, Ruiz-Lozano P, Rodriguez-Esteban C, Yonei-Tamura S, Magallon J, Chandraratna RA, Chien K, Blumberg B, Evans RM, Belmonte JC. Multiple left-right asymmetry defects in Shh(-/-) mutant mice unveil a convergence of the shh and retinoic acid pathways in the control of Lefty-1. Proc Natl Acad Sci U S A. 1999;96(20):11376-81. PubMed PMID: 10500184; PMCID: PMC18041.

Ruiz-Lozano P, Hixon ML, Wagner MW, Flores AI, Ikawa S, Baldwin AS, Jr., Chien KR, Gualberto A. p53 is a transcriptional activator of the muscle-specific phosphoglycerate mutase gene and contributes in vivo to the control of its cardiac expression. Cell Growth Differ. 1999;10(5):295-306. PubMed PMID: 10359011.

Nakamura T, Ruiz-Lozano P, Lindner V, Yabe D, Taniwaki M, Furukawa Y, Kobuke K, Tashiro K, Lu Z, Andon NL, Schaub R, Matsumori A, Sasayama S, Chien KR, Honjo T. DANCE, a novel secreted RGD protein expressed in developing, atherosclerotic, and balloon-injured arteries. J Biol Chem. 1999;274(32):22476-83. PubMed PMID: 10428823.

Marszalek JR, Ruiz-Lozano P, Roberts E, Chien KR, Goldstein LS. Situs inversus and embryonic ciliary morphogenesis defects in mouse mutants lacking the KIF3A subunit of kinesin-II. Proc Natl Acad Sci U S A. 1999;96(9):5043-8. PubMed PMID: 10220415; PMCID: PMC21813.

Kuo H, Chen J, Ruiz-Lozano P, Zou Y, Nemer M, Chien KR. Control of segmental expression of the cardiac-restricted ankyrin repeat protein gene by distinct regulatory pathways in murine cardiogenesis. Development. 1999;126(19):4223-34. PubMed PMID: 10477291.

Kimber WL, Hsieh P, Hirotsune S, Yuva-Paylor L, Sutherland HF, Chen A, Ruiz-Lozano P, Hoogstraten-Miller SL, Chien KR, Paylor R, Scambler PJ, Wynshaw-Boris A. Deletion of 150 kb in the minimal DiGeorge/velocardiofacial syndrome critical region in mouse. Hum Mol Genet. 1999;8(12):2229-37. PubMed PMID: 10545603.


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Barak Y, Nelson MC, Ong ES, Jones YZ, Ruiz-Lozano P, Chien KR, Koder A, Evans RM. PPAR gamma is required for placental, cardiac, and adipose tissue development. Molecular Cell. 1999;4(4):585-95. PubMed PMID: 10549290.

Ruiz-Lozano P, Smith SM, Perkins G, Kubalak SW, Boss GR, Sucov HM, Evans RM, Chien KR. Energy deprivation and a deficiency in downstream metabolic target genes during the onset of embryonic heart failure in RXRalpha-/- embryos. Development. 1998;125(3):533-44. PubMed PMID: 9425147.

Doevendans PA, Ruiz-Lozano P, van Bilsen M. Hunting down nucleic acid binding factors in the cardiovascular system. Cardiovasc Res. 1998;38(2):301-15. PubMed PMID: 9709391.

Cao L, Zhang L, Ruiz-Lozano P, Yang Q, Chien KR, Graham RM, Zhou M. A novel putative protein-tyrosine phosphatase contains a BRO1-like domain and suppresses Ha-ras-mediated transformation. J Biol Chem. 1998;273(33):21077-83. PubMed PMID: 9694860.

Ruiz-Lozano P, Doevendans P, Brown A, Gruber PJ, Chien KR. Developmental expression of the murine spliceosome-associated protein mSAP49. Dev Dyn. 1997;208(4):482-90. doi: 10.1002/(SICI)1097-0177(199704)208:4<482::AID-AJA4>3.0.CO;2-E. PubMed PMID: 9097020.

Harris AN, Ruiz-Lozano P, Chen YF, Sionit P, Yu YT, Lilly B, Olson EN, Chien KR. A novel A/T-rich element mediates ANF gene expression during cardiac myocyte hypertrophy. J Mol Cell Cardiol. 1997;29(2):515-25. PubMed PMID: 9140811.

Fu Y, Ruiz-Lozano P, Evans SM. A rat homeobox gene, rNKx-2.5, is a homologue of the tinman gene in Drosophila and is mainly expressed during heart development. Dev Genes Evol. 1997;207(5):352-8. doi: 10.1007/s004270050122. PubMed PMID: 27747432.

de Lecea L, Ruiz-Lozano P, Danielson PE, Peelle-Kirley J, Foye PE, Frankel WN, Sutcliffe JG. Cloning, mRNA expression, and chromosomal mapping of mouse and human preprocortistatin. Genomics. 1997;42(3):499-506. doi: 10.1006/geno.1997.4763. PubMed PMID: 9205124.

Ruiz-Lozano P, de Lecea L, Buesa C, Perez de la Osa P, LePage D, Gualberto A, Walsh K, Pons G. The gene encoding rat phosphoglycerate mutase subunit M: cloning and promoter analysis in skeletal muscle cells. Gene. 1994;147(2):243-8. PubMed PMID: 7926808.

Perez de la Ossa P, Grana X, Ruiz-Lozano P, Climent F. Isolation and characterization of cofactor-independent phosphoglycerate mutase gene from maize. Biochem Biophys Res Commun. 1994;203(2):1204-9. PubMed PMID: 8093040.


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BOOK CHAPTERS Nguyen, V.T.B., Chen. J., Ruiz-Lozano, P., Chien, K.R. (1998). Genetic control of muscle gene regulation: The MLC-2a paradigm for ventricular heart chamber specification, maturation and morphogenesis. Heart Development. Eds R,P. Doevendans, P.A., Bronsaer, R., Ruiz-Lozano, P., vanDantzig, JM., van Bilsen, M. (1998) Ventricular expression of the atrial regulatory myosin light chain gene. In Cardiovascular Gene Expression. Kluwer Academic Publishers. Ruiz-Lozano, P. and Chien, K.R. (1998) Retinoid signaling: insights from genetically engineered mice. In Cardiovascular Gene Expression. Kluwer Academic Publishers. Doevendans, P.A., Ruiz-Lozano, P., vanBilsen, M. (1998) Hunting down nucleic acid binding factors in the cardiovascular system. Cardiovascular Research 38: 301-315 Ruiz-Lozano, P., Raya, A., Chien. K.R., Izpisua-Belmonte, JC. (2000) Cardiac Laterality and Congenital Heart Disease. in Molecular Basis of Cardiovascular Disease. A companion to Braunwald’s Heart Disease. pg 238-248 Ruiz-Lozano, P., Nakamura, T., Chien, KR. (2001) Genes del desarrollo y enfermedad cardiaca. Rev. Española de Cardiologia 54: 1439-1445 Manner, J. and Ruiz-Lozano, P. (2008) Development and function of the epicardium. In: Advances in Developmental Biology: Cardiovascular Development vol. 18; pp 334-357 (Bodmer, R., Ed). Elsevier Serpooshan V, Ruiz-Lozano P. Ultra-rapid Manufacturing of Engineered Epicardial Substitute to Regenerate Cardiac Tissue Following Acute Ischemic Injury. Methods in molecular biology. 2014;1210:239-48. Epub 2014/09/01. doi: 10.1007/978-1-4939-1435-7_18. PubMed PMID: 25173173.

INVITED SPEAKER 2001 Barcelona Biochemistry and Molecular Biology Lecture Series. U. B.

2001 Tokyo Pediatric Cardiology. Tokyo Women’s Medical University

2003 Boston Weinstein Conference for Cardiac Development

2004 San Diego Advances in Endocrinology

2004 La Jolla, US Cardiovascular Science Conference. University of California, San Diego

2004 Philadelphia Thomas Jefferson Medical Center

2004 Tucson, AZ University of Arizona

2004 Palo Alto Stanford University, Division of Cardiovascular Medicine

2005 Palo Alto Stanford University, Division of Pediatrics

2005 Geneva University of Geneva

2005 Utrecht Heart and Lung Symposium, Utrecht University, The Netherlands


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2005 Steamboat Keystone Symposium on Heart Failure

2006 Boston Massachusetts General Hospital

2006 Boston Wyeth Pharmaceuticals, U.S.A

2008 Palo Alto Stanford University. Cardiopulmonary Series, U.S.A

2008 Philadelphia Childrens Hospital Phyladelphia, University of Pennsylvania, U.S.A

2008 New Orleans AHA Scientific Sessions, U.S.A

2009 Huston UT Southwestern Physiology Seminar Series, U.S.A

2009 Cologne 9th Cologne Conference on Heart Failure, Germany

2009 San Diego Cardiovascular Science Conference, UCSD, U.S.A

2009 San Francisco Fetal Cardiac Interventions Symposium, Stanford University, U.S.A

2010 Barcelona Biomed Seminars Plenary Program, IRB and IBMB-CSIC, Spain

2010 Oxford Wellcome Trust Seminars, Oxford University, United Kingdom

2010 San Diego Heart Failure Society of America, U.S.A

2011 London Imperial College. British Heart Foundation Center of Research

Excellence United Kingdom

2012 Madrid Centro Nacional Investigaciones Cardiologicas (CNIC), Spain

2014 Shanghai Chinese National Compound Library Center, China

2014 London Keynote Speaker. British Heart Foundation Tissue Engineering

Consortium, United Kingdom

2014 Shanghai Fudan University , China

2015 La Jolla Tenth Annual Stem Cells Meeting, La Jolla, CA

2015 Chania Eagean Conference Systems Biology, Greece

2015 Buenos Aires Conference for Tissue Regeneration and Degeneration, Argentina

2015 Utrecht University Medical Center Utrecht, Netherlands

2015 New York City Mount Sinai School of Medicine

2016 Chicago Northwestern University. Guest Speaker, Department of Pharmacology

2016 Munich 3rd Munich Conference on Cardiac Development: “From current

understanding to new regenerative concepts“

2016 Utrecht Keynote Speaker, 5th Utrecht Stem Cell Conference

2016 Bristol, UK Invited Lecture

2016 Barcelona Invited Speaker. Center of Regenerative Medicine in Barcelona


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2016 Tokyo Keynote Speaker, Society of Cardiovascular Endocrinology and

Metabolism 20th Annual Meeting.

2017 Crete 12th International Conference on Pathways, Networks, and Systems

Medicine

2017 Oklahoma City University of Oklahoma Health Sciences Center

2017 Los Angeles Chemical Engineering and Materials Science. University of Southern

California

2017 Milan Nature Conference on Regeneration

2018 Switzerland Ascona Workshop on Cardiomyocyte Biology

2018 London, UK Imperial College London

TRAINEES

Name Training period

Current position

Tiffany Neal, MBs 1999-2001 Researcher, Invitrogen

Judith Canicio, Ph.D. 1999-2000 Pharmacist, Barcelona

Jacob Andrade, MD, PhD 2000-2003 Medical Resident, Univ. Southern California

Esther Merki, PhD 2002-2005 Professor Cuyamaca College El Cajon, CA

Diego Franco, PhD 2004 Professor, University of Jaén

Francisco Hernández, PhD 2004 Staff Scientist, CNIC Madrid

Mónica Zamora, Ph.D 2003-2007 Research Associate, CIBERMED Barcelona

M. Cecilia Scimia, MD, PhD 2007-2010 Associate Medical Director, Takeda Pharmaceuticals

Ke Wei, PhD 2008-2015 Professor, Tiangjin University Shanghai

Saugata Ray, PhD 2008- 2010 Business Development, Genoptix/Novartis

Cecilia Hurtado, PhD. 2009-2010 Instructor, Stanford University

Nicole Ling, MBs 2007-2010 Founder and CEO, UTBodies Inc, China

Marta Diez-Cuñado, PhD 2010-2013 Postdoctoral Associate, SBP Medical Discovery

Vahid Serpooshan, PhD 2011-2014 Assistant Professor, Georgia Tech and Emory University


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FUNDING Current Research Support

2018 – 2021: NIH R44HL140649, SBIR : “IND-enabling Epicardial Biopharmaceutical in the Diabetic Heart “. Total Award $3,941,096

Pending Support (in consideration zone)

2019-2022: NIH R44 HL144269, SBIR: “Development of a microsphere formulation for intracoronary delivery of a regenerative biopharmaceutical to the heart”. Total Award $4,257,941

Completed Research Support as Principal Investigator 1994-1995: CIRIT Post-doctoral fellowship, Generalitat of Catalunya.

Goals: To determine the transcriptional mechanisms of cardiac specific gene regulation.

1995-1997: Post-Doctoral fellowship. Ministerio Espanol de Educacion y Ciencia. Goals: Analysis of the factors that determine vitamin A dependent cardiac morphogentic defects.

• 1998-2000: Individual NRSA, National Heart Lung and Blood Institute. NIH Goals: Analysis of the effect of systemic mutation of RXRα target genes.

2000-2002: Beginning Grant-in-Aid American Heart Association Western States Affiliate

Goals: Development of adenovirus mediated technology for use in cardiac development.

2002-2013: NIH, R01 HL 65484 Genetic Ablation of Epicardial Function in the Mouse

Goals: This project analyzed of a retinoid/wnt/fgf pathway in cardiac growth and morphogenesis

2006-2008: Wyeth Pharmaceuticals APJ in cardiac protection.

Goals: This study approaches the role of the apelin/APJ system in hypertrophy.

2008-2014: NIH, R01HL086879 Genetic Rescue of Retinoid-Induced Dysfunction

Goals: Here we will analyze the molecular mechanism by which depression of GPCR signaling restores function in retinoid deficient mouse models

2013-2014: Seed Award Stanford Cardiovascular Institute

Engineered Epicardium In Large Animal Models Of Heart Failure Goals: Application of a new device for heart failure treatment

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