C1 C2

PI-PLC1-CYFP+PI-PLC1-NYFP

PI-PLC1+Gα-Q223L

PI-PLC1+Gα-Q223L

B2 B3B1

C2 C3C1

ER-mCherry Merge

PM-mCherry Merge

PI-PLC1-CYFP +PI-PLC1-NYFP

A1 A2

D1

PI-PLC1-CYFP +Clo3-NYFP

Figure S8. BiFC interactions in tobacco leaf epidermal cells.A: PI-PLC1-CYFP and PI-PLC1 -NYFP dimerization on ER and PM; B:co-localization of GA3-Q223L-CYFP and PI-PLC1-NYFP interaction to ER; C:co-localization of GA3-Q223L-CYFP and PI-PLC1-NYFP to PM; Twenty cells of GA3-Q223L-CYFP and PI-PLC1-NYFP BiFC interaction have higher YFP flourecent compered to GA3-CYFP and PI-PLC1-NYFP BiFC interaction; D:the localization of the interaction between the two PI-PLC1-CYFP and Clo3-NYFP apparent by ER network structure seen in the upper focal planes of the cell. Scale bar = 24 µM