pg 547
DESCRIPTION
LDH converts pyruvate to lactate, restoring the pool of NAD +. pg 547. What about when pyruvate leaves the cytoplasm…? . ?. pg 547. Glycerol 3-P shuttle . fig19-30. Malate-aspartate shuttle . fig19-29. Malate-aspartate shuttle . Malate-aspartate shuttle viewed as e- carrying process . - PowerPoint PPT PresentationTRANSCRIPT
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Lecture 11 Slides
fig19-16
electron transport chain
fig19-17
proton-motive force
23.6 kJ/mole H+
the coupling question…
chemiosmotic hypothesis!
the coupling question…
chemiosmotic hypothesis!
fig19-19
oxidative phosphorylation experiment
addthis
measurethis
fig19-20
oxidative phosphorylation experiment
addthis
measurethis
ADP + Pi
ATP
fig19-20
fig19-20
oxidation and phosphorylation are coupled!!!
fig19-20
oxidation and phosphorylation are coupled!!!
oxidative phosphorylation experiment
addthis
measurethis
ADP + Pi
ATP
oxidative phosphorylation experiment
ADP + Pi
ATPremovethis
thisstops
oxidative phosphorylation experiment
ADP + Pi
ATP
blockATPase
thisstops
weak acid uncouplers
low H+
high H+
fig19-21
Weak aciduncouplers
fig19-21
Chemical uncouplers
fig19-21
Chemical uncouplers
Chemical uncoupler experiment
fig19-20
From ETC and OxPhos
Protongradientexperiment
fig19-22
From ETC and OxPhos
ATP synthase: the F1 subunit
fig19-23
ATP and ADP are in rapid equilibriumin the F1 ATPase
Energetics of ATP and ADP in F1 ATPase…
fig19-24
fig19-25
The “binding-change” model for ATP synthase
fig19-25
The “binding-change” model for ATP synthase
fig19-26
The “binding-change” model for ATP synthase
fig19-25
The F1 complex of the ATPase…
The F0F1 complex
fig19-25
The F0F1 complex
fig19-25
membrane
soluble
The F0F1 complex of the ATPase…
fig19-25
The F0F1 complex of the ATPase…
fig19-25
A direct test of the rotary mechanism
fig19-25 Noji et al. 1997
A direct test of the rotary mechanism
Snapshots from the direct test of rotary mechanism…
fig19-27
The movie version of the book
an ancient mode of ATP production
Bacterial generation of H+ gradient by respiratory chain
“There’s more than one way to shrink a gradient…”
fig19-39
Inner membrane translocases (or transporters)
fig19-28
Glycerol 3-P shuttle
fig19-30
eeeeek!
Malate-aspartate shuttle
fig19-29
NADH NAD+
Malate-aspartate shuttle
Malate-aspartate shuttle
X
Malate-aspartate shuttle viewed as e- carrying process
OAA OAA
malate malate
NADH
NAD+
NADH
NAD+
Malate-aspartate shuttle viewed as e- carrying processwith set of transaminations between OAA/Asp, and aKG/Glu
malate malate
NADH
NAD+
NADH
NAD+
OAA OAA
AspAsp
aKGaKG
Glu Glu
Malate-aspartate shuttle viewed as e- carrying process
malate malate
NADH
NAD+
NADH
NAD+
OAA OAA
with set of transaminations between OAA/Asp, and aKG/Glu
OAA OAA
AspAsp
aKGaKG
Glu Glu
with set of transaminations between OAA/Asp, and aKG/Glu
OAA OAA
AspAsp
aKGaKG
Glu Glu
with set of transaminations between OAA/Asp, and aKG/Glu
OAA OAA
AspAsp
aKGaKG
Glu Glu
NH3 groupfrom Asp to
aKG
NH3 groupfrom Glu to
OAA
Malate-aspartate shuttle viewed as e- carrying processwith set of transaminations between OAA/Asp, and aKG/Glu
malate malate
NADH
NAD+
NADH
NAD+
OAA OAA
AspAsp
aKGaKG
Glu Glu
Malate-aspartate shuttle
fig19-29
Nature’selectric blanket
fig19-34thermogenin
regulation of ATP producingpathways
fig19-33
fig19-33
regulation of ATP producingpathways
fig19-33
regulation of ATP producingpathways