peroxidase gene polymorphism in lens and vicia species
TRANSCRIPT
Poster presentations / Current Opinion in Biotechnology 24S (2013) S48–S143 S127
highest amount of total polyphenolic compounds was detected incultivar Negra; single polyphenols as follow: gallic acid 33.14 mg/L,caftaric acid 26.71 mg/L in pulp, (+) catechin 65.71 mg/L and (−) epi-catechin 61.48 mg/L in seeds and myricetin 5.18 mg/L, quercetin3.78 mg/L and kaempferol 2.73 in grape skin.
http://dx.doi.org/10.1016/j.copbio.2013.05.402
Response of Vitis vinifera cell cultures on stress caused bymethyl jasmonate
Martin Sák 1,2, Ivana Dokupilová 3,4, Daniel Mihálik 3, JánSajbidor 1
1 Institute of Biotechnology and Food Science, Faculty of Chemical andFood Technology, Slovak University of Technology, Bratislava, Slovakia2 Department of Analytical Chemistry, Plant Production Research Cen-ter, Piest’any, Slovakia3 Department of Molecular Biology, Plant Production Research Center,Piest’any, Slovakia4 Institute of Biochemistry, Nutrition and Health Protection, Facultyof Chemical and Food Technology, Slovak University of Technology,Bratislava, SlovakiaE-mail address: [email protected] (M. Sák).
Plant cell culture represent potential source of secondary metabo-lites which cannot be produced by microorganism and chemicalsynthesis. However, only a few of plant cell cultures producethese compounds in commercially amount. In vitro cell culturesof Vitis vinifera cv. Váh were inoculated with commercial elicitormethyl jasmonate for detection of polyphenol production dependon cultivation time and elicitor concentration. Influence of differentelicitor amounts was evaluated by high performance liquid chro-matography with diode array detection in 1, 4, 6, and 8 days afterelicitation. Cellular responses to different concentration of methyljasmonate (0.2 mM, 0.4 mM and 0.8 mM) included rapid alkalinisa-tion, polyphenol production and cell death. Variously distributedphenolic compounds including (+) catechin, (−) epicatechin, p-coumaric acid, syringaldehyde, rutin and p-coumaric acid detectedin cells were statistically evaluated depended on time and elicitorconcentration. Overall, total production of polyphenols increasedin treated cells compared with untreated; the addition of methyljasmonate had a generally increasing effect to production and thehighest amount was measured in 8 day after elicitation reaching7.83 mg/g(fw). On the other hand control cells displayed a higheramount of viable cells. When elicitors were supplied, cell growthwas reduced although cell viability did not show a massive celllysis. The alkalinization of the culture medium measured duringthe each period of polyphenol sampling was almost constant inthe control (difference ±0.01), but decreased about average 0.15 intreated cultures after of each cultivation day.
http://dx.doi.org/10.1016/j.copbio.2013.05.403
Development important forms of soft spring wheat in NorthKazakhstan
Assiya Turganbayeva, Oksana Hapilina, Galina Shek, AlmagulKakimzhanova
National Center for Biotechnology, Astana, KazakhstanE-mail address: [email protected] (A. Turganbayeva).
Spring wheat is the main exporting crop in Kazakhstan. The majorshare of grain sold on the world market is the grain of spring wheatgrown in North Kazakhstan, where the area under this crop is85%. The aim of this study was to create new varieties of wheat
through biotechnology and conventional breeding with directionalselection for resistance to drought and disease. Objects were vari-eties of wheat in Kazakhstan. In 2011, for state testing were givennew varieties of spring wheat — Ak Orda and Kazakhstan 20. Anew variety of Kazakhstan 20 refers to a kind of Lutescens. It wasobtained by in vitro from hybrid of Akmola 2/Akmola 40. The lineis drought resistant and responsive to moisture conditions. Aver-age yield for 2009–2011 on the steam background was 20.6 t/ha. In2011, through the manufacturing variety trials exceeded the yieldstandard on 5.6 t/ha. A new variety of Ak Orda refers to Albidumvariety. It was obtained by the cell selection method with culturefiltrate of the Alternaria alternata of constant line of hybrid origin.In the manufacturing variety trials, exceeded the yield standard on2.9 t/ha. The line is resistant to ‘black germ’. Studies from 2000 to2011 on the selection in vitro with adding selective agents and testprogeny of regenerated wheat revealed competitive lines to createnew varieties of spring wheat.
http://dx.doi.org/10.1016/j.copbio.2013.05.404
Peroxidase gene polymorphism in lens and Vicia species
Duygu Sari 1, Cengiz Toker 1, Nedim Mutlu 2
1 Department of Field Crops, Faculty of Agriculture, Akdeniz University,TR-07070 Antalya, Turkey2 Department of Agricultural Biotechnology, Faculty of Agriculture,Akdeniz University, TR-07070 Antalya, TurkeyE-mail address: [email protected] (C. Toker).
Peroxidases are organized into three classes as intracellular perox-idases, secretory fungal peroxidases and plant peroxidases. Plantperoxidases belong to a multigene family and play important rolesin many stress-related interactions, such as pathogen infection,insect tolerance, salt tolerance, auxin degradation, cell wall ligni-fications, tissue suberization, and plant senescence. They posseshighly conserved domains allowing oligonucleotide primers to bedesigned to amplify DNA sequences coding for peroxidases fromplants with unsequenced genomes. Thanks to conserved structure,plant peroxidases are used for classification of plant species. In thisstudy, peroxidase gene-based polymorphism among Lens speciesincluding L. culinaris Medik., L. orientalis (Boiss.) Ponert, L. tomento-sus Ladiz., L. odemensis Ladiz., L. lamottei Czefr., L. ervoides (Brign.)Grande and L. nigricans (M.Bieb.) Godr. and Vicia L. comprising Viciamontbretii (Fisch. and Mey.) Davis and Plitmann, and correlationbetween peroxidase markers were conducted. Thirty Lens and 2Vicia species were evaluated using 22 peroxidase specific primers.PCR amplification indicated high polymorphism between speciesbased on peroxidases. Especially for 7 primer combinations yieldedhigher polymorphisms than the others. These results indicated thatperoxidase gene family can be used to study genotypic diversityand evolutionary relationships.
http://dx.doi.org/10.1016/j.copbio.2013.05.405
Resistance gene analog polymorphism in Lens species
Duygu Sari 1, Nedim Mutlu 2, Cengiz Toker 1
1 Department of Field Crops, Faculty of Agriculture, Akdeniz University,TR-07070 Antalya, Turkey2 Department of Agricultural Biotechnology, Faculty of Agriculture,Akdeniz University, TR-07070 Antalya, TurkeyE-mail address: [email protected] (C. Toker).
Plant resistance genes (R-gene) play an important role inplant–pathogen recognition and these genes present several highly