performance work statement fisheries domain …...national marine fisheries service (nmfs) alaska...

Performance Work Statement Fisheries Domain

National Oceanic and Atmospheric Administration (NOAA) National Marine Fisheries Service (NMFS)

Alaska Fisheries Science Center (AFSC) Auke Bay Lab Juneau-EMA Program

Fisheries Biologists Field and Laboratory Support

Requirement #1 EMA Program

Background The National Marine Fisheries Service (NMFS) conducts fisheries oceanographic research in the Southeast Alaska Waters, Gulf of Alaska (GOA), Bering sea, Chukchi Sea, and Beaufort Sea with a wide variety of interdisciplinary collaborators in order to understand the influence of biophysical forcing and climate on the recruitment of marine fish and fisheries: including the effects of ocean currents on transporting juvenile fish to nursery grounds; the effects of food availability on productivity of marine fish populations; aging and life history conditions for salmonids, and the energetic requirements for overwinter survival of marine fish.

This task order provides for the expertise to aid NOAA biologists in collecting and processing samples and data for supporting ongoing fisheries oceanographic research projects on vessels, in the laboratory and at field stations. The tasks will involve sample processing for fish and biological oceanography, sample inventories, support work for surveys, laboratory analyses, at sea fish collections and data entry. Contractor support will participate in fisheries oceanographic surveys and potentially go to a remote field location to support data collection. Duties onboard the survey vessels include: assisting with the coordination and preparation of sampling gear; identifying and measuring fish; data entry; and assisting with oceanographic sampling. Participation in group planning meetings will be required.

The objective of this task order is to obtain support services to assist in conducting research activities in and around Alaskan marine environments.

Historically this Requirement had support services:

• In biology or field of study relating and experience relating to the specific task order. • Experience on research vessels and chartered fishing boats. • Salmon spawning, Coded wire tagging and reading, and scale pressing and reading for

age • Experience in various laboratory fish processing techniques.

Task & Deliverables The Contractor shall provide the following services throughout the period of performance and complete the following projects and deliver them to the NMFS Project Leader no later than the end of the period of performance for each line item:

In addition to the tasks listed below, the Contractor will coordinate with their NMFS project leader on assignments and work products. This position consists mainly of laboratory and field support duties.

Base Period

Task A 1. Description: Performs various laboratory duties for processing chlorophylls and fish

samples. Protocols for each type of laboratory procedure are provided and must be adhered to make data comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.

2. Deliverable: For each procedure the deliverable is either the vial or packaged sample itself or the data file generated from the processing procedure.

Task B

1. Description: Participate in annual fieldwork which includes a research survey and or work at a field station. Duties include assisting with zooplankton, biological and physical oceanography (operating CTDs), fish sampling, fish tagging, and fish spawning. All sampling protocols will be provided by the chief scientist or field party chief prior to reaching the vessel or at the field station.

2. Deliverable: Physical presence aboard the vessel or in the field satisfies the requirement.

Task C

1. Description: Participate in all staging and de-staging of fieldwork. Participate in all cleaning, assemblage (preparation), and dissemination of all related elements to research projects, includes data, frozen samples, supplies and equipment. Specifically maintenance on oceanographic sensors, zooplankton nets and electronic fish measuring system.

2. Deliverable: Inventory lists prior to field packing, freezer inventory pre and post cruise, and final inventory list post field season. Plus any data requests must be emailed to provide proof of dissemination.

Task D

1. Description: Participate in annual Chinook salmon tagging with coded wire tags. 250,000 Chinook smolts are tagged each year at a remote field station. The time frame required to complete the task is 1-3 weeks. Deliverable: Physical presence at the field station with experience in CWT tagging and salmon smolt adipose clipping satisfies the requirement.

• Otolith removals from fish heads, NTE 500 • Obtaining Chlorophyll values from processed water filters, NTE 1500 • Removal of fish stomachs and innards for diet and energetic work, NTE 700 • Fish scale pressing, reading and aging, NTE 5000 • Removal of tissue pieces for genetics, NTE 3000 • Perform bomb calorimetry on fish samples, NTE 2000

2. Deliverable: Physical presence at the field station participating in CWT tagging and salmon smolt adipose clipping satisfies the requirement.

Option Year I Task A

2. Description: Performs various laboratory duties for processing chlorophylls and fish samples. Protocols for each type of laboratory procedure are provided and must be adhered to make data comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.


Task B



Task C



Task D

3. Description: Participate in annual Chinook salmon tagging with coded wire tags. 250,000 Chinook smolts are tagged each year at a remote field station. The time frame required to complete the task is 1-3 weeks.

4. Deliverable: Physical presence at the field station with experience in CWT tagging and salmon smolt adipose clipping satisfies the requirement.



Option Year II Task A



Task B



Task C



Task D




Option Year III Task A



Task B



Task C



Task D




Option Year IV Task A



Task B



Task C



Task D




Government Furnished Resources

The Government will provide all necessary information, data and documents to the Contractor for work required under this contract.

The Contractor shall use Government furnished facilities, property, equipment and supplies only for the performance of work under this contract, and shall be responsible for returning all Government furnished facilities, property, and equipment in good working condition, subject to normal wear and tear.

The Government will provide to the Contractor the following equipment, supplies, and similar, necessary to conduct these services and anticipates facility and security access as follows:

Services Contractor will work primarily: ☒ On-Site ☐ Off-Site

Services Contractor requires access to NOAA facilities:

☒ Required ☐ Not Required

Services Contractor requires a PIV or CAC: ☒ Required ☐ Not Required

Services Contractor requires a NOAA email address:


Services Contractor requires use of DOC-Owned IT equipment (laptop or desktop computer)


Services Contractor requires non-Government IT equipment to connect to a DOC network:

☐ Required ☒ Not Required

Services Contractor requires access to non-public DOC/NOAA data:


Services Contractor will be provided on-site office space with incidental Government Property (desk, chair, phone, and other similar equipment that remains onsite, per FAR 45.000 (5)

☒ Provided ☐ Not Provided

For Services Contractors doing laboratory work, the government will provide typical lab supplies and equipment for use:


For Services Contractors doing field work, the Government will provide typical field equipment, gear, and supplies for use:


Description of other access or equipment requirements not already addressed above:

Government will provide aircraft safety training and large vessel cold water safety training.

Training

Training is anticipated and authorized for this order. The anticipated training includes:

a. Large Vessel Marine Safety Training: specific training related to working on large vessels b. First Aid and CPR: due to the remoteness of the work, everyone needs basic safety training c. Small boat and Fire Arm Safety Training: due to the remoteness of the field station and wildlife interactions d. Small aircraft aviation evacuation training: due to the transportation necessary to get to the field station

Travel Travel is anticipated and authorized for this task order. The current projected itinerary is as follows. Departure and return dates will be finalized prior to travel and may vary from scheduled itinerary:

Trip 1: Summer of 2020, one overnight trip to meet a survey vessel for the Base Period

Trip 2: April of 2020, three week trip to remote field location for the Base Period

Trip 3: Summer of 2021, one overnight trip to meet a survey vessel for the Option Year I

Trip 4: April 2021, one three week trip to remote field location for the Option Year I

Trip 5: Summer of 2022, one overnight trip to meet a survey vessel for the Option Year II

Trip 6: April 2022, one three week trip to remote field location for the Option Year II

Trip 7: Summer of 2023, one overnight trip to meet a survey vessel for the Option Year III

Trip 8: April 2023, one three week trip to remote field location for the Option Year III

Trip 9: Summer of 2024, one overnight trip to meet a survey vessel for the Option Year IV

Trip 10: April 2024, one three week trip to remote field location for the Option Year IV

Each trip to meet a vessel will likely occur from Sitka, Dutch Harbor, Nome, Barrow, and or Juneau but could encompass other locations throughout Alaska. Travel will include 2 nights lodging in port city to embark and 1 night lodging in port city to disembark from the vessel. Reimbursement costs to include lodging, M&IE, and transportation. All expenses for travel to the remote field location at Little Port Walter Station will be provided by the government.

Special and/or Additional Requirements

The following additional aspects are / are not anticipated during the performance of this task order:

Domestic / U.S. Travel ☒ Anticipated ☐ Not Anticipated

Foreign / Overseas Travel ☐ Anticipated ☒ Not Anticipated

Specialized Air Travel / Aerial Surveys: ☒ Anticipated ☒ Not Anticipated

Sea Days (Deployed at Sea): ☒ Anticipated ☐ Not Anticipated

Dockside / Harbor Side Work: ☒ Anticipated ☐ Not Anticipated

Scuba Diving / Underwater Work: ☐ Anticipated ☒ Not Anticipated

Remote Working Conditions (e.g. Worksite lacks basic services, has isolated living conditions, or similar):

☒ Anticipated ☐ Not Anticipated

Other: {Provide description below} ☐ Anticipated ☐ Not Anticipated

Description of anticipated special and / or additional requirements not already described above:

Chartered flights to remote field station at Little Port Walter near Sitka Alaska.

Operational Hours

During the performance of this task order, the Contractor shall perform during the normal operating hours of the facility, five (5) days per week for the performance period. No more than 40 hours per week unless in field work status.

Place of Performance

1. On-Site at NMFS office located at 17109 Point Lena Loop Road, Juneau Alaska

Period of Performance

The base period is expected to be from September 30, 2019 through September 29, 2020 with four (4) option years through September 29, 2024.

Qualifications Must have the following minimum qualifications: At least 12 credits of college level course work in a field related to the requirements of the specific task order with emphasis in fisheries, biology, oceanography, social science, natural science, mathematics, or hydrology.

Additional required qualifications include:

a. The applicant must have experienced at least 10 continuous days at sea on a research vessel or commercial fishing boat in Alaskan Waters. b. The applicant must have at least three years’ experience working with fisheries samples in a laboratory setting. c. The applicant must have at least 1 year of experience with reading and aging scales for at least 2 species of salmon. d. The applicant must have experience in participating in 1 tagging event for salmon using coded wire tags.



Alaska Fisheries Science Center (AFSC) Auke Bay Lab Juneau-RECA Program

Biology Laboratory Technician Support

Requirement #2

Background The National Marine Fisheries Service (NMFS) conducts fisheries oceanographic research in the Southeast Alaska Waters, Gulf of Alaska (GOA), Bering sea, Chukchi Sea, and Beaufort Sea with a wide variety of interdisciplinary collaborators in order to understand the influence of biophysical forcing and climate on the recruitment of marine fish and fisheries including: aging and life history conditions for salmonids, the energetic requirements for overwinter survival of marine fish, and how growth, energy storage, and metabolism interact to regulate the distribution and abundance of fish stocks during the recruitment phase.

This task order provides for the expertise to aid NOAA biologists in processing samples and data for supporting ongoing fisheries oceanographic research projects in the laboratory. The tasks will involve sample processing of fish and zooplankton including: sample inventories, laboratory analyses, and data entry.

The objective of this task order is to obtain laboratory support services to assist in processing fish and zooplankton samples collected during research activities in and around Alaskan marine environments.


• In biology or field of study relating and experience relating to the specific task order.

• Experience in various laboratory fish processing techniques. • Experience analyzing samples with a PARR micro-bomb calorimeter • Experience dissecting and decoding coded-wire-tags



Base Period a. Task A 1. Description: Performs various biological laboratory duties for processing fish and

zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.


Option Year I

a. Task A 1. Description: Performs various biological laboratory duties for processing fish and


• Inventory fish arriving at laboratory, NTE 1250 • Measure length and weight of fish, NTE 1000 • Removal of otoliths, NTE 875 • Removal of tissue pieces for genetics, NTE 875

• Inventory fish arriving at laboratory, NTE 1250 • Measure length and weight of fish, NTE 1000 • Removal of otoliths, NTE 875 • Removal of tissue pieces for genetics, NTE 875 • Removal of tissue pieces for RNA/DNA, NTE 75 • Removal of tissue pieces for Isotopes, NTE 625 • Removal of fish stomachs and innards for diet and energetic work, NTE 875 • Homogenize fish, NTE 875 • Analyze fish with PARR semi-microbomb calorimeter for energy density,

NTE 750 • Removal and decoding of coded-wire-tags, NTE 1000

• Removal of tissue pieces for RNA/DNA, NTE 75 • Removal of tissue pieces for Isotopes, NTE 625 • Removal of fish stomachs and innards for diet and energetic work, NTE 875 • Homogenize fish, NTE 875 • Analyze fish with PARR semi-microbomb calorimeter for energy density,



Option Year II

a. Task A 1. Description: Performs various biological laboratory duties for processing fish and



Option Year III a. Task A

1. Description: Performs various biological laboratory duties for processing fish and zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.

• Inventory fish arriving at laboratory, NTE 1250 • Measure length and weight of fish, NTE 1000 • Removal of otoliths, NTE 875 • Removal of tissue pieces for genetics, NTE 875 • Removal of tissue pieces for RNA/DNA, NTE 75 • Removal of tissue pieces for Isotopes, NTE 625



• Removal of fish stomachs and innards for diet and energetic work, NTE 875 • Homogenize fish, NTE 875 • Analyze fish with PARR semi-microbomb calorimeter for energy density,



Option Year IV a. Task A 1. Description: Performs various biological laboratory duties for processing fish and





The Contractor shall only use Government furnished facilities, property, equipment, and supplies for the performance of work under this contract, and shall be responsible for returning all Government furnished facilities, property, and equipment in good working condition, subject to normal wear and tear.





















☐ Provided ☐ Not Provided


Government will provide laboratory safety training including hazardous chemical training.

Training


e. Laboratory safety training including hazardous chemical training.

Travel Travel is not anticipated for this task order.



Domestic / U.S. Travel ☐ Anticipated ☒ Not Anticipated


Specialized Air Travel / Aerial Surveys: ☐ Anticipated ☒ Not Anticipated

Sea Days (Deployed at Sea): ☐ Anticipated ☒ Not Anticipated

Dockside / Harbor Side Work: ☐ Anticipated ☒ Not Anticipated



☐ Anticipated ☒ Not Anticipated



Operational Hours

During the performance of this task order, the Contractor shall perform during the normal operating hours of the facility, five (5) days per week for the performance period. No more than 40 hours per week.




The base period is expected to be from September 30, 2019 through September 29, 2020 with four (4) option years through September 29, 2024.

Qualifications

Selected individual(s) must have the following minimum qualifications: At least 12 credits of college level course work in a field related to the requirements of specific task order with emphasis in fisheries, oceanography, biology, social science, natural science, mathematics, or hydrology.

Additional required qualifications include:

At least one year of experience in a biology laboratory. Experience processing larval or juvenile marine fish, including: measuring lengths and

weights; dissecting and removing stomach contents and storing in formalin for identification; otolith, fin, and scale removal;

Experience homogenizing fish tissues in preparation for chemical analysis; Experience operating a PARR brand semi-micro bomb calorimeter to obtain energy

density values including calibration, maintenance, and troubleshooting; Experience with proper handling and storage of formalin and isopropyl/ethyl alcohol; Experience organizing field and laboratory data including performing quality control

procedures on both raw and electronic;




Chemistry Laboratory Technician Support

Requirement #3



The objective of this task order is to obtain biological and chemistry laboratory support services to assist in processing fish and zooplankton samples collected during research activities in and around Alaskan marine environments.

Historically this Requirement had support services: In chemistry or field of study relating and experience relating to the specific task order. Experience operating a LECO thermogravimetric analyzer Experience in SPV lipid analysis using an Accelerated Solvent Extractor 350 Experience in RNA/DNA analysis using a fluorometric 96-well plate reader Experience in various laboratory fish processing techniques.


In addition to the tasks listed below, the Contractor will coordinate with their NMFS project leader on assignments and work products. This position consists mainly of laboratory support duties.

Base Period a. Task A 1. Description: Performs various chemistry laboratory duties for processing fish and

zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data is comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.

2. Deliverable: For each procedure the deliverable is either the vial, tray, or packaged sample itself or the data file generated from the processing procedure.

b. Task B


• Inventory fish arriving at laboratory, NTE 500 • Measure length and weight of fish, NTE 400 • Removal of otoliths, NTE 350 • Removal of tissue pieces for genetics, NTE 350 • Removal of tissue pieces for RNA/DNA, NTE 30 • Removal of tissue pieces for Isotopes, NTE 250

• Measure moisture and ash of fish samples using a LECO thermogravimetric analyzer, NTE 3500

• Perform SPV lipid analysis on fish and zooplankton samples using an Accelerated Solvent Extractor 350, NTE 800

• Perform RNA/DNA analysis on fish samples using a fluorometric 96-well plate reader, NTE 300

• Prepare fish samples for isotope analysis using a semi-micro or micro balance to load samples and standards in a tin foil capsule, NTE 3500

• Removal of fish stomachs and innards for diet and energetic work, NTE 350 • Homogenize fish, NTE 350 • Analyze fish with a PARR semi-microbomb calorimeter for energy density,

NTE 750


Option Year I

a. Task A 1. Description: Performs various chemistry laboratory duties for processing fish and

zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data is comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.


b. Task B


• Inventory fish arriving at laboratory, NTE 500 • Measure length and weight of fish, NTE 400 • Removal of otoliths, NTE 350 • Removal of tissue pieces for genetics, NTE 350 • Removal of tissue pieces for RNA/DNA, NTE 30 • Removal of tissue pieces for Isotopes, NTE 250 • Removal of fish stomachs and innards for diet and energetic work, NTE 350 • Homogenize fish, NTE 350 • Analyze fish with a PARR semi-microbomb calorimeter for energy density,

NTE 750






Option Year II a. Task A

1. Description: Performs various chemistry laboratory duties for processing fish and zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data is comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.


b. Task B



Option Year III

a. Task A 1. Description: Performs various chemistry laboratory duties for processing fish and

zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data is comparable between years for long term data


NTE 750





sets. Number of samples typically processed varies each year which is determined by the amount of research projects.






b. Task B



Option Year IV a. Task A

1. Description: Performs various chemistry laboratory duties for processing fish and zooplankton samples. Protocols for each type of laboratory procedure are provided and must be followed to ensure data is comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.




NTE 750




b. Task B











NTE 750


















Training


g. Laboratory safety training including hazardous chemical training.














Operational Hours





The base period is expected to be from September 30, 2019 through September 29, 2020 with four (4) option years: (1) September 30, 2020 through September 29, 2021; (2) September 30, 2021 through September 29, 2022; (3) September 30, 2022 through September 29, 2023; (4) September 30, 2023 through September 29, 2024.

Qualifications Must have the following minimum qualifications: At least 12 credits of college level course work in a field related to the requirements of specific task order with emphasis in chemistry, fisheries, oceanography, biology, social science, natural science, mathematics, or hydrology.

At least 1 year of experience chemistry or related analytical laboratory processing

biological samples. Experience conducting RNA/DNA analysis; Experience homogenizing fish tissues in preparation of chemical analysis; Experience with proper handling and storage of methanol, chloroform, dichloromethane,

sulfuric acid, and phosphoric acid; Knowledge and experience with SPV lipid analysis, using an organic solvent system

(Methylene chloride or chloroform/methanol). Experience preparing samples for bomb calorimetry; Experience working in a research laboratory, following established Standard Operating

Procedures (SOP's) and adhering to Good Laboratory Practice (GLP) cleanliness guidelines.

Ability to perform Quality Control (QC) according to fixed parameters. Experience documenting instrumental and processing difficulties in detailed narratives.





Requirement #4


This task order provides for the expertise to aid NOAA biologists in processing samples and data for supporting ongoing fisheries oceanographic research projects in the laboratory. The tasks will

involve sample processing of fish and zooplankton including: sample inventories, laboratory analyses, and data entry.



In biology or field of study relating and experience relating to the specific task order. Experience in various laboratory fish processing techniques. Experience analyzing samples with a PARR micro-bomb calorimeter Experience dissecting and decoding coded-wire-tags



Base Period b. Task A 2. Description: Performs various biological laboratory duties for processing fish and





Option Year I

b. Task A





Option Year II

b. Task A 2. Description: Performs various biological laboratory duties for processing fish and



Option Year III b. Task A

2. Description: Performs various biological laboratory duties for processing fish and zooplankton samples. Protocols for each type of laboratory procedure are provided



and must be followed to ensure data comparable between years for long term data sets. Number of samples typically processed varies each year which is determined by the amount of research projects.




Option Year IV b. Task A 2. Description: Performs various biological laboratory duties for processing fish and





























Training


f. Laboratory safety training including hazardous chemical training.














Operational Hours





3. The base period is expected to be from September 30, 2019 through September 29, 2020 with four (4) option years: (1) September 30, 2020 through September 29, 2021; (2) September 30, 2021 through September 29, 2022; (3) September 30, 2022 through September 29, 2023; (4) September 30, 2023 through September 29, 2024.










Requirement #5

Background The National Marine Fisheries Service (NMFS) conducts fisheries oceanographic research in the Southeast Alaska Waters, Gulf of Alaska (GOA), Bering sea, Chukchi Sea, and Beaufort Sea with a wide variety of interdisciplinary collaborators in order to understand the influence of

Qualifications Must have the following minimum qualifications: At least 12 credits of college level course work in a field related to the requirements of specific task order with emphasis in fisheries, oceanography, biology, social science, natural science, mathematics, or hydrology. Additional required qualifications include:

biophysical forcing and climate on the recruitment of marine fish and fisheries including: aging and life history conditions for salmonids, the energetic requirements for overwinter survival of marine fish, and how growth, energy storage, and metabolism interact to regulate the distribution and abundance of fish stocks during the recruitment phase.




In biology or field of study relating and experience relating to the specific task order. Experience in various laboratory fish processing techniques. Experience analyzing samples with a PARR micro-bomb calorimeter Experience dissecting and decoding coded-wire-tags



Base Period c. Task A 3. Description: Performs various biological laboratory duties for processing fish and





Option Year I

c. Task A 3. Description: Performs various biological laboratory duties for processing fish and



Option Year II

c. Task A 3. Description: Performs various biological laboratory duties for processing fish and







Option Year III c. Task A


Deliverable: For each procedure the deliverable is either the vial or packaged sample

itself or the data file generated from the processing procedure.

Option Year IV c. Task A 3. Description: Performs various biological laboratory duties for processing fish and






Deliverable: For each procedure the deliverable is either the vial or packaged sample itself or the data file generated from the processing procedure.

























Training


g. Laboratory safety training including hazardous chemical training.














Operational Hours



On-Site at NMFS office located at 17109 Point Lena Loop Road, Juneau Alaska


The base period is expected to be from September 30, 2019 through September 29, 2020 with four (4) option years: (1) September 30, 2020 through September 29, 2021; (2) September 30, 2021 through September 29, 2022; (3) September 30, 2022 through September 29, 2023; (4) September 30, 2023 through September 29, 2024.






Qualifications Must have the following minimum qualifications: At least 12 credits of college level course work in a field related to the requirements of specific task order with emphasis in fisheries, oceanography, biology, social science, natural science, mathematics, or hydrology. Additional required qualifications include:

performance work statement fisheries domain …...national marine fisheries service (nmfs) alaska...

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