PCRBy:

Cody Alveraz Ted Dobbert

Morgan Pettit

What is PCR?

PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of magnitude, creating thousands to millions of copies of DNA sequences.

It was developed in 1983 by Kary Mullis.

Procedures of PCR Denaturation: At 201.2 F degrees the

double stranded DNA melts and opens into 2 pieces of single-stranded DNA.

Annealing: At medium temperatures, at around 54 degrees Celsius, the primers pair up with the single-stranded “template”. On the small length of double-stranded DNA, the polymerase attaches and starts copying the template.

Procedures of PCR (cont.) Extension: AT 72 degrees Celsius, where

the polymerase works best, and DNA building blocks complementary to the template are coupled to the primer, making a double-stranded DNA molecule.

With one cycle a segment of double-stranded DNA template is amplified into two separate pieces of double stranded DNA.

What is good about PCR? PCR is good technique because its not

expensive, rapid and a good way to get millions of copies of DNA.

PCR Process Diagram

PCR Machine

Links: http://

www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm

http://www.youtube.com/watch?v=eEcy9k_KsDI