pcr and electrophoresis. techniques of recombinant dna technology multiplying dna in vitro: the...
TRANSCRIPT
![Page 1: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/1.jpg)
PCR and Electrophoresis
![Page 2: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/2.jpg)
Techniques of Recombinant DNA Technology
Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR)
VIDEO CLIP◦Large number of identical molecules of DNA
produced in vitro◦Critical to amplify DNA in variety of situations
Epidemiologists use to amplify genome of unknown pathogen
Amplified DNA from Bacillus anthracis spores in 2001 to identify source of spores
2
![Page 3: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/3.jpg)
Techniques of Recombinant DNA Technology
Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR)◦Repetitive process consisting of three steps
Denaturation Priming Extension
◦Can be automated using a thermocycler
3
![Page 4: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/4.jpg)
Polymerase chain reaction (PCR)
4
Figure 8.5a
![Page 5: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/5.jpg)
Polymerase chain reaction (PCR)
5
Figure 8.5b
![Page 6: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/6.jpg)
Techniques of Recombinant DNA Technology
6
![Page 7: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/7.jpg)
Techniques of Recombinant DNA Technology
Separating DNA Molecules: Gel Electrophoresis and the Southern Blot◦Gel electrophoresis
Separates molecules based on electrical charge, size, and shape
Allows scientists to isolate DNA of interest Negatively charged DNA drawn toward positive electrode Agarose makes up gel; acts as molecular sieve Smaller fragments migrate faster and farther than larger
ones Determine size by comparing distance migrated to
standards
7
![Page 8: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/8.jpg)
Gel electrophoresis
8
Figure 8.6
![Page 9: PCR and Electrophoresis. Techniques of Recombinant DNA Technology Multiplying DNA in vitro: The Polymerase Chain Reaction (PCR) VIDEO CLIP ◦Large number](https://reader036.vdocuments.us/reader036/viewer/2022082709/56649f495503460f94c6b444/html5/thumbnails/9.jpg)
GEL ELECTROPHORESIS VIDEO
Video of our lab’s gel electrophoresisElectrophoresis Results video
9