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The Future of the Biofuel Industry: Lessons Learned

World Biofuels Markets – BrazilSeptember 28, 2011

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We are Amyris

No Compromise® Renewable Fuels and

Specialty Chemicals for a sustainable world

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CUSTOMERS

RENEWABLE CHEMICALS AND FUELSANY FEEDSTOCK

PRODUCERS

INDUSTRIAL SYNTHETIC BIOLOGY PLATFORM

PARTNERS

SummaryA Disruptive Platform for the Renewable Chemicals and Fuels Industry

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Lessons Learned …

?… or Key Success Factors for a New Biofuel

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Millions of Hectares (2009)

BRAZIL TOTAL AREA 851.2

TOTAL ARABLE LAND 329.9

1. Total Crop Land 59.8

Soybean 21.6

Corn 14.4

Sugarcane 8.1

2. Pastures 158.8

3. Available Area 111.3

Sources: UNICA, NIPE-Unicamp, IBGE, CTC, CanaSat, see http://www.dsr.inpe.br/canasat/ 5

1 Hectare = 2.5 acres(100m x 100m)

Sugarcane occupies 2.5% of

Brazil’s arable land

Zoning law Zoning law

64,7 Million Hectares for Sugarcane8x

… plus productivity improvement and better use of sugarcane leaves, tops and bagasse

1. Feedstock Availability and SustainabilitySugarcane in Brazil has large expansion potential in area & productivity

CropsPasture Sugar Cane

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Soybean Biodiesel

Ethanol withIgnition improver

Amyris Diesel

900 <->1000 km

5.100 <-> 6800 km

7600<->9500 km

6.460 Liters/year

4.180 liters/year

468 Liters/year

Source: UNICAMP, Embrapa, Amyris, SPTRANS, Mercedes-Benz, NOTE: Amyris Diesel figures consider technology at target productivity and yields.

2. Proven Land Use EfficiencyAmyris Diesel and other common alternatives in Brazil

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Isoprenoid Pathway

YEAST CELL

3. Enabling TechnologyFrom sugars to hydrocarbons!

Artemisinic Acid

H

H

O

HO

p450

CPR

ADS

Anti-Malarial DrugSugar Syrup

Diesel & Chemical PrecursorFarnesene Synthase

Farnesene

GPPS

PS

Jet / Gasoline PrecursorsIsoprene

IS

Ethanol

X

SUGAR SOURCE

Amyris modifies microorganisms to enable production of 1,000s of Target Molecules

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Lab2 Liters

Pilot Plant300 Liters

Pilot Plant 300 L

Demo Plant 5000 L

Production 200 - 600K L

Lab 2L scale

Successful Commercialization takes years of preparation!

Fermentation Scale-Up

AnalyticsKnowledge

Management

Rational StrainDesign

RandomMutagenesis

StrainEngineering

Deconvolution

Screening

4. Proven Scale-Up CapabilityU.S. and Brazilian sites working in sync

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No Compromise® = No changes required on Custom Behavior, Engine, Vehicle, Storage, Distribution System, etc.

5. Fuel with Drop-In PropertiesAmyris diesel: a pure hydrocarbon (C15H32)

No Sulfur

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Petroleum

Biodiesel

Renewable Diesel

Amyris Diesel

110 112 114 116 118 120 122 124 126 128 130

Energy Density Higher value provides increase

fuel economy

Petroleum

Biodiesel

Renewable Diesel

Amyris Diesel

-80 -70 -60 -50 -40 -30 -20 -10 0

Cold Filter Pour Point (°C)Lower value improves cold weather

engine operation

Petroleum

Biodiesel

Renewable Diesel

Amyris Diesel

300 350 400 450 500 550

Lubricity (µ) Lower value provides decrease

engine wear

Petroleum

Biodiesel

Renewable Diesel

Amyris Diesel

-15 -10 -5 0 5 10 15 20 25

Engine Emission Impact (NOx)Lower value better for environment

and health

Baseline NOx level

NOx reduction

Petroleum

NOx increase

Sources: NREL, Neste, MWM

6. Proven Technical Benefits Performance Comparison: Amyris & other diesel fuel

Measured in 1000 x BTU/Gal

S50

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7. Proven Environmental Benefits (Local) Reduction on main toxic tailpipe gases

Note: Fossil diesel as reference. Different engines tested according to different legislations for emissions

Engine Blend%

Reduction* PM Reduction* NOx

MB OM 924 10% -5% 0

MB OM 924 30% -5,4% -1,3%

MB OM 924 100% -13% -6%

MB OM 906 100% -25% -15%

Detroit Series 60 5% 0 -2%

Detroit Series 60 20% -1% -5%

Detroit Series 60 50% -6% -12%

Cummins ISB 6.7L 20% -0,4% -2%

Cummins ISB 6.7L 35% -2,8% -7,5%

MWM-Int. MF 7.2H 50% -35% -2,6%

MWM-Int. MF 7.2H 100% -47% -4,4%

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Petroleum Gasoline

Sugarcane EtOH (Brazil)

Corn EtOH (US)

Petroleum Diesel

Amyris Diesel (Brazil)

0.0

20.0

40.0

60.0

80.0

100.0

Lif

e-c

yc

le G

HG

Em

iss

ion

s (

g/M

J)

Amyris Diesel shows a reduction in GHG’s …

>90% reduction

Computed by LCA using CARB methodology

7. Proven Environmental Benefits (Global)Reduction of GHG emissions

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Engine TestingValidating Amyris Diesel through fuel system, engine and vehicle manufacturer tests

Brazil

8. Tested Results by Key Industry PlayersAmyris: expediting validation through Industry Partnerships

Fleet TestingConfirming Amyris Diesel fuel quality and durability through robust road demonstration

Registration & ApprovalsAchieving market entry by securing government registration and approvals

Brazil

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Sugar Cane plantations

Oil refineries

Rain forest and wet lands

Potential for sugar cane

Source: edata from IBGE, Embrapa, Unicamp, e ANP.

400(São Paulo) 300

(Rio de Janeiro)

100(Belo Horizonte)

Diesel demand ( million liters)

Legend

100(Recife)

100(Brasília)

100(Curitiba)

100(Salvador)

50(Fortaleza)

50(Porto Alegre)

50(Cuiaba)

50(Natal)

50(Manaus)

50(Belém)

50(Guarulhos)

50(Goiânia)

50(Campinas)

50(São Luís)

São Paulo 400Rio de Janeiro 300Belo Horizonte 100

Recife 100Salvador 100Brasília 100Curitiba 100

Campinas 50Guarulhos 50

Porto Alegre 50Cuiaba 50Goiania 50Manaus 50Belem 50

São Luis 50Fortaleza 50

Natal 50TOTAL 1700

liters per year ( x1000 m³)

9. Market Proximity to Feedstock Fuel consumption by metropolitan buses in Brazil

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Summary of Lessons Learned …

1. Feedstock cost, availability and sustainability

2. Proven land use efficiency

3. Enabling technology available

4. Proven scale-up capability

5. Fuel with “drop-in” properties

6. Proven technical benefits

7. Proven environmental benefits (local and global)

8. Tested results by key industry players

9. Market proximity to feedstock

… or Key Success Factors for a new Biofuel ?

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Thank You !!!