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RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 1 of 12 PATHOLOGY SPECIMEN TUBES QUICK REFERENCE GUIDE : 2014 Edition Expires February 2015 FOR THE MOST UP TO DATE INFORMATION AND FOR TESTS THAT ARE NOT LISTED HERE PLEASE CHECK THE INTRANET SITE AT: http://rhhlas26/path_tests/lab_default.asp or www.pathologysouth.com.au Group 1 : The following tests can be performed on a single Red Top 4mL SST tube SST 4mL (Red top with gel) ACE AFP (alpha Foetoprotein) Alcohol alpha 1 antitrypsin Amikacin B12/folate ß2-Microglobulin C3, C4 CA-125 C-reactive protein (CRP) CK Caeruloplasmin Calcium group Carbamazepine CEA Cholesterol Digoxin Ferritin Fructosamine Gentamicin Glucose ( sample must be rec'd < 4 hours post collection.) Haptoglobin HCG HDL IPG Iron (Iron Studies) LDH LFT Lipase Lithium Methotrexate Osmolality Paracetamol Phenobarb Procalcitonin Phenytoin PSA Rheumatoid Factor Salicylate Theophylline Tobramycin Transferrin Triglyceride Troponin U&E Urate Valproate Vancomycin

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RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 1 of 12

PATHOLOGY SPECIMEN TUBES

QUICK REFERENCE GUIDE : 2014 Edition Expires February 2015

FOR THE MOST UP TO DATE INFORMATION AND FOR TESTS THAT ARE NOT LISTED HERE PLEASE CHECK THE INTRANET

SITE AT: http://rhhlas26/path_tests/lab_default.asp or www.pathologysouth.com.au

Group 1 : The following tests can be performed on a single Red Top 4mL

SST tube

SST 4mL (Red top with gel)

ACE

AFP (alpha Foetoprotein)

Alcohol

alpha 1 antitrypsin

Amikacin

B12/folate

ß2-Microglobulin

C3, C4

CA-125

C-reactive protein (CRP)

CK

Caeruloplasmin

Calcium group

Carbamazepine

CEA

Cholesterol

Digoxin

Ferritin

Fructosamine

Gentamicin

Glucose ( sample must

be rec'd < 4 hours post

collection.)

Haptoglobin

HCG

HDL

IPG

Iron (Iron Studies)

LDH

LFT

Lipase

Lithium

Methotrexate

Osmolality

Paracetamol

Phenobarb

Procalcitonin

Phenytoin

PSA

Rheumatoid Factor

Salicylate

Theophylline

Tobramycin

Transferrin

Triglyceride

Troponin

U&E

Urate

Valproate

Vancomycin

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 2 of 12

Each of the following GROUPS of tests requires its own

5 mL White Top tube with gel, irrespective of other test

and sample requirements.

Group 2

TSH, TFT, Insulin, C-peptide, Prolactin, FSH,

Estradiol, Vitamin D.

Group 3 Serology

Viral PCR eg HCv, HBV

Group 4

ANA

ENA

ANCA

Copper

EPG

Selenium

Zinc

Plain 5mL (White Top with gel)

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 3 of 12

NB: Fill to thick black arrow

Sodium Citrate 3.5 mL (Blue Top)

EDTA 4mL (Pink Top)

FBC

Retics

Hb

Separate Tube Required for these tests

Haemoglobin Alc (HbA1c)

Homocysteine (on ice)

BNP (must arrive within

4hrs)

Cyclosporin

Tacrolimus

Lead

Cadmium

PCR

APTT

D-Dimers

Fibrinogen

INR

Coags

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 4 of 12

EDTA - Transfusion - 9mL (Purple Top) OR

EDTA - Transfusion - 2mL (Purple Top)

Blood Group and Hold

Crossmatch

Specimens for blood group or crossmatch

must be hand labelled and signed by the

person;

• who collected the specimen

• who witnessed the specimen collection

Samples with pre-printed labels will not be

processed.

Complete all fields of the dedicated

Transfusion Request Form.

Adults

Paediatrics

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 5 of 12

NB: Always fill the GREY TOP tube to the thick black

line

Sodium Fluoride/EDTA 2mL (Grey Top)

NB: Fill to line, if problems with filling

then also send a filled 4ml Pink Top

Do not stick label onto inner clear tube

Black Rubber Top Tube with Additive

Lactate

Glucose

NB. Glucose can be measured

on a Red Top SST tube

provided it is received by the

lab within 4 hours of collection

ESR

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 6 of 12

9 ml Yellow Top ACD Tube

7 ml Navy Top Tube

Flow Cytometry

Studies (Yellow

Top and Pink Top)

Separate Tube Required

for HIV Loads = 9ml

Purple Top (dedicated

sample) for this test

Aluminium

Chromium

Cobalt

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 7 of 12

9 ml or 4 ml Green Top Tube

9 ml or 4 ml Green Top Tube

Ammonia

Send on ice

Advise Lab. Before collection, Ext. 8775

Chromosome studies/Karyotype

FISH for 22q

Adult-9mL Child-4mL

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 8 of 12

PCR : Separate Tube required

EDTA 4mL (Pink Top)

Swab (Blue Cap)

Swab

MC & S only

See database for all

other swabs

Fragile X DNA test

Prader Willi Syndrome / Angelman Syndrome

Molecular Karyotyping / Microarray

Sample must be received in laboratory no later than 2pm Thursday.

Enquiries: Ext 8297

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 9 of 12

Paediatric Tubes – Haematology and Transfusion

EDTA 2mL (Purple Top) OR EDTA 0.5mL (Pink Top) ( Neonates )

Fill to the line

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 10 of 12

Paediatric Tubes - Coagulation

Sodium Citrate 1mL (Blue) Sodium Citrate 2mL (Blue Top) - Coags

Fill to thick black line

Bullet with gel 1mL - Biochemistry ( Neonates )

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 11 of 12

SPECIMEN LABELLING

All sample tubes must show the following details :

Patient's surname and given name(s)

Date of Birth

Unit Record Number

Date and time of collection

Ward

Clear labelling of tubes is essential and handwritten labels are

preferred. If patient sticky labels are used then they must be

the small ones and placed longitudinally so that the contents

and fill level are still visible for the laboratory staff to check.

All specimens must be accompanied by a completed Request

Form signed by the Doctor and with the Doctor's name written

in block capitals to avoid misidentification of the Requestor.

RHH Doctors must also use their stamp with their Provider

Number on it.

Specimens for blood group or crossmatch must be hand labelled

and signed by

• the person who collected the specimen

• the person who witnessed the specimen collection

Blood group or crossmatch samples with pre-printed labels

will not be processed.

Complete all fields of the dedicated Transfusion Request Form.

SPECIMEN TRANSPORT AND DELIVERY

All samples must be transported in a sealed Biohazard bag with the

sample in the sealable section and the Request Form in the separate

pocket.

Blood gas or ionised calcium samples are transported in the

syringe in which the blood was taken. The needle must be removed

before transport and replaced with a protective cap. If transported

in the vacuum tube system the samples must be packed with ice

and double bagged.

Vacuum Tube System

Samples may be sent in the appropriate canisters via the vacuum

tube system which has stations situated throughout the hospital.

The numbers for Pathology are 84161 or 84162.

RHH PATHOLOGY : PHLEBOTOMY SERVICE and PATHOLOGY SOUTH SPECIMEN COLLECTION CENTRE

Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: Jan 2014 Page 12 of 12

All specimens must be in Biohazard bags.

Blood cultures should be wrapped in bubble wrap and bagged and

only one set of blood culture tubes should be put in one canister.

Certain samples such as those for Platelet Function Analysis must

not be sent by Vacuum Tube—check the Pathology Specimen

Database if you are uncertain about sending a specimen via the

Vacuum Tube System.

Personal Delivery to Pathology.

Samples should be brought to Pathology Reception which is

located on the 1st floor of the D block (Gaha Wing) in the area

near the lifts adjacent to the Clinical School.

PATHOLOGY RESULTS

All reports other than HIV reports are available electronically via

the RHH Intranet Digital Medical Record.

https://dmrprimary.dhhs.tas.gov.au/udr/app/aut

henticate.html?returnheader=true

Internet Explorer is available on PCs in the clinical areas and

should be used to access this information; it should be

unnecessary for you to phone the labs for Routine Results or

for Routine Specimen Container Information. Hard copy

reports are produced throughout the day and distributed to the

Wards via the internal mail system.

TESTS AVAILABLE DATABASE - Direct URLs

http://rhhlas26/path_tests/lab_default.asp

or

http://pathologysouth.com.au/information-for-clinicians/test-

information/