Diapositiva 1

Pathogen inactivationNew progress

Antonio Piga

Hemoglobinopathies CentreDepartment of Clinical and Biological SciencesUniversity of Torino, Italyantonio.piga@unito.it1Mechanism of action of pathogen inactivation

Mechanism of action of pathogen inactivation (PI). Amotosalen (S-59) is a synthetic psoralen thatinhibits nucleic acid replication through UVA-light-mediated covalent addition to nucleic acids. Left: themolecule penetrates cells, viruses, bacteria, or other pathogens and seeks out DNA or RNA. 2nd left:amotosalen intercalates between the base pairs. 2nd right: once illuminated by UVA light, amotosalen formsmonoadducts between pyrimidine bases. Right: another photon of light enables the molecule to form crosslinks(diadducts) between DNA or RNA strands (from ref. 73).2Requirements for pathogen inactivationEffective to eliminate pathogensMaintaining the quality of blood products for transfusionSafeSimple and cost-effective to implement in the preparation of blood componentsAdapted from Busch MP, TRANSFUSION 2006;46:1624-16401:100,0001:1,000,0001:10,0001:1001:1000Risk per Unit