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10/24/2017 PASADENA Amgen Biotech Experience Materials Request Form (2 pages) Each teacher at the participating school must complete and return this form for his/her classes Name of Teacher and Email address: ___________________________________________________________ Name of School: ____________________________ Your Phone: _______________ School Phone: _________________ List other teachers doing the labs during this time block and their email addresses: ___________________________ ________________________________________________________________________________________________ Reservation/Pick-up Date: ___________________ Equipment + Supplies ______ Supplies only ______ If you are receiving Supplies Only, do you have MiniOne system electrophoresis boxes? Yes______ No _______ Select the Pathway your students will be doing and fill in the requested numbers Complete Genetic Engineering Sequence (Labs 1, 2, 3, 4, 5, and 6) Description: This laboratory sequence tells the “complete” biotechnology story from moving a gene of interest into an engineered expression vector. Unlike the Abridged Genetic Engineering sequence, this sequence involves the digestion of pARA and pKAN-R followed by a ligation lab to produce recombinant plasmids. This represents the most challenging set of protocols. This sequence includes Purifying the Fluorescent Protein (Lab 6), but you may choose not to include this lab. Next to each class name please enter the number of classes doing this lab series. ______Number of students running this sequence Intro Bio. ______ Advanced/Honors Bio. ______ ______Number of groups running this sequence AP Bio. ______ Biotech ______ i.e. AP Bio. __3__ Biotech _1__ Other: (Name and number of classes) ____________ OPTIONAL: Lab 6 Yes_____ No_____ Date for Lab 5__________ Abridged Genetic Engineering Sequence (Labs 1, 2a, 4a, 5a, and 6) Description: This sequence omits the pARA/pKAN-R digestion and ligation of restriction fragments, and starts with the rfp gene already ligated into the expression vector. Students run a plasmid digest and electrophoresis separating pARA-R restriction fragments. This was written to shorten the labs required to get to the protein purification step and to increase the likelihood of students succeeding in producing red transformants. The sequence requires less teacher preparation time. This sequence includes Purifying the Fluorescent Protein (Lab 6), but you may choose not to include this lab Next to each class name please enter the number of classes doing this lab series. ______Number of students running this sequence Intro Bio. ______ Advanced/Honors Bio. ______ ______Number of groups running this sequence AP Bio. ______ Biotech ______ i.e. AP Bio. __3__ Biotech _1__ Other: (Name and number of classes) ____________ OPTIONAL: Lab 6 Yes_____ No_____ Date for Lab 5A__________ Focus on Bacteria Sequence (Labs 1, 5b, and 6) Description: The focus of this sequence is on the importance of plasmids as vectors for use in genetic engineering and the ability of bacteria to utilize the genetic information of the plasmid to produce a protein product. It is not only important to produce the protein, it is equally important that the protein be capable of being collected and purified. This sequence includes Purifying the Fluorescent Protein (Lab 6), but you may choose not to include this lab. Next to each class name please enter the number of classes doing this lab series. ______Number of students running this sequence Intro Bio. ______ Advanced/Honors Bio. ______ ______Number of groups running this sequence AP Bio. ______ Biotech ______ i.e. AP Bio. __3__ Biotech _1__ Other: (Name and number of classes) ____________ OPTIONAL: Lab 6 Yes_____ No_____ Date for Lab 5B__________ Introduction to Biotechnology (Lab 1) Description: The focus of this lab is to enable the students to become proficient in basic biotechnology lab skills. It includes using the micropipette and running a gel electrophoresis. Next to each class name please enter the number of classes doing this lab series. ______Number of students running this sequence Intro Bio. ______ Advanced/Honors Bio. ______ ______Number of groups running this sequence AP Bio. ______ Biotech ______ i.e. AP Bio. __3__ Biotech _1__ Other: (Name and number of classes) ____________ *SEE NEXT PAGE*

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Page 1: PASADENA Amgen Biotech Experience Materials Request …abe-la.org/uploads/3/4/8/3/34833906/pasadena_center_abe_documents... · Each box has pictures to help you find items and to

10/24/2017

PASADENAAmgenBiotechExperienceMaterialsRequestForm(2pages)Eachteacherattheparticipatingschoolmustcompleteandreturnthisformforhis/herclassesNameofTeacherandEmailaddress:___________________________________________________________NameofSchool:____________________________YourPhone:_______________SchoolPhone:_________________Listotherteachersdoingthelabsduringthistimeblockandtheiremailaddresses:___________________________________________________________________________________________________________________________Reservation/Pick-upDate:___________________Equipment+Supplies______Suppliesonly______IfyouarereceivingSuppliesOnly,doyouhaveMiniOnesystemelectrophoresisboxes?Yes______No_______SelectthePathwayyourstudentswillbedoingandfillintherequestednumbers

CompleteGeneticEngineeringSequence(Labs1,2,3,4,5,and6)Description:Thislaboratorysequencetellsthe“complete”biotechnologystoryfrommovingageneofinterestintoanengineeredexpressionvector.UnliketheAbridgedGeneticEngineeringsequence,thissequenceinvolvesthedigestionofpARAandpKAN-Rfollowedbyaligationlabtoproducerecombinantplasmids.Thisrepresentsthemostchallengingsetofprotocols.ThissequenceincludesPurifyingtheFluorescentProtein(Lab6),butyoumaychoosenottoincludethislab. Nexttoeachclassnamepleaseenterthenumberofclassesdoingthislabseries.______Numberofstudentsrunningthissequence IntroBio.______Advanced/HonorsBio.____________Numberofgroupsrunningthissequence APBio.______Biotech______ i.e.APBio.__3__Biotech_1__ Other:(Nameandnumberofclasses)____________ OPTIONAL:Lab6Yes_____No_____DateforLab5__________ AbridgedGeneticEngineeringSequence(Labs1,2a,4a,5a,and6)Description:ThissequenceomitsthepARA/pKAN-Rdigestionandligationofrestrictionfragments,andstartswiththerfpgenealreadyligatedintotheexpressionvector.StudentsrunaplasmiddigestandelectrophoresisseparatingpARA-Rrestrictionfragments.Thiswaswrittentoshortenthelabsrequiredtogettotheproteinpurificationstepandtoincreasethelikelihoodofstudentssucceedinginproducingredtransformants.Thesequencerequireslessteacherpreparationtime.ThissequenceincludesPurifyingtheFluorescentProtein(Lab6),butyoumaychoosenottoincludethislab

Nexttoeachclassnamepleaseenterthenumberofclassesdoingthislabseries.______Numberofstudentsrunningthissequence IntroBio.______Advanced/HonorsBio.____________Numberofgroupsrunningthissequence APBio.______Biotech______ i.e.APBio.__3__Biotech_1__ Other:(Nameandnumberofclasses)____________ OPTIONAL:Lab6Yes_____No_____DateforLab5A__________

FocusonBacteriaSequence(Labs1,5b,and6)Description:Thefocusofthissequenceisontheimportanceofplasmidsasvectorsforuseingeneticengineeringandtheabilityofbacteriatoutilizethegeneticinformationoftheplasmidtoproduceaproteinproduct.Itisnotonlyimportanttoproducetheprotein,itisequallyimportantthattheproteinbecapableofbeingcollectedandpurified.ThissequenceincludesPurifyingtheFluorescentProtein(Lab6),butyoumaychoosenottoincludethislab.

Nexttoeachclassnamepleaseenterthenumberofclassesdoingthislabseries.______Numberofstudentsrunningthissequence IntroBio.______Advanced/HonorsBio.____________Numberofgroupsrunningthissequence APBio.______Biotech______ i.e.APBio.__3__Biotech_1__ Other:(Nameandnumberofclasses)____________ OPTIONAL:Lab6Yes_____No_____DateforLab5B__________

IntroductiontoBiotechnology(Lab1)Description:Thefocusofthislabistoenablethestudentstobecomeproficientinbasicbiotechnologylabskills.Itincludesusingthemicropipetteandrunningagelelectrophoresis. Nexttoeachclassnamepleaseenterthenumberofclassesdoingthislabseries.______Numberofstudentsrunningthissequence IntroBio.______Advanced/HonorsBio.____________Numberofgroupsrunningthissequence APBio.______Biotech______ i.e.APBio.__3__Biotech_1__ Other:(Nameandnumberofclasses)____________ *SEENEXTPAGE*

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10/24/2017

ColonyPCRDescription:Thepurposeofthislabistoconfirmthecellsproducingredfluorescentproteinhavebeentransformedwiththeplasmidcarryingtherfpgene,pARA-R,usingafundamentalmolecularbiologytool,PCR. Nexttoeachclassnamepleaseenterthenumberofclassesdoingthislab._____Numberofstudentsrunningthislab IntroBio____Advanced/HonorsBio______ _____Numberofgroupsrunningthislab APBio______Biotech______

i.e.APBio.__3__Biotech_1__ Other:(Nameandnumberofclasses)_________________*WouldyouprefertoborrowStudentGuides?Ifyes,howmany?___________**Doyouhaveanincubatoratyourschool?Yes_________NO_________***Ifyouarerequesting“SuppliesOnly”,doyouneedanyadditionalequipment(e.g.,extrapipets,waterbath,etc.)?NEW!!!!!ADDITIONALREQUIREDINFORMATION!!!!1.SchoolDistrict(Spelloutthename):__________________________2.SchoolAddress(CompleteincludingSchoolstreetaddress,SchoolcityANDZip:_______________________________

3.IsthisYOURfirstyeardoingABE?Ifyouarereturningafterayearortwoawayfromtheprogram,pleaseconsideryourselfasnew.____________________4.IsthisyourSCHOOL’SfirstyeardoingABE?_________________________ImportantNotes:TheStudentGuidesandTeacher’sGuide(neweditions2015)canbedownloadedfromthe“Curriculum”pageatwww.amgenbiotechexperience.com.Besuretodownloadthecorrectsequence,ifnotdownloadingtheentireguides.*****RETURNTHISFORMIMMEDIATELY.YOUWILLBECONTACTEDTHREETOFOURWEEKSBEFOREYOURPIICKUPDATEANDASKEDTOUPDATELABSERIESANDSTUDENTNUMBERS.

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10/24/2017

AmgenBiotechExperienceKitInstructorChecklist

ContactInformation:SherryTsai,So.CalDirectorAmgenBiotechExperience,[email protected]

GraceG.Montgomery,SiteCoordinator,PasadenaDistributionCenter,[email protected]

AllAmgenBiotechExperiencelabsaretobeperformedusingallappropriatesafetyattireandprecautionsrequiredina

scienceclassroomlaboratory.Pleaserefertoyourschooldistrict’srulesregardingthese.

UponPICKUPofthekit:*ChecktoseethatyouhavereceivedallthesupplieslistedonyoursupplyspreadsheetASAP.CallGraceifyouaremissinganything.Asyouunpacktheboxes,notehowtheyarepackedtomakeiteasierforyouwhenyouneedto

repackthem.Eachboxhaspicturestohelpyoufinditemsandtorepacktheminthecorrectbox.

*Besuretostoresuppliesattheappropriatetemperature.Thesupplyspreadsheetiscolorcodedtohelpyou.

*ThekitincludesaTeacherNotebookwhichcontainsallthelabupdates,equipmentmanualandhelpfultips.Please

refertothisandthewebsitewww.amgenbiotechexperience.comespeciallyforrecentlabupdatesandteacherresources.

*PleasereturnALLunusedreagents,emptybottlesandsupplies.

*Pleasetakephotosofyourbestplatesonthetransilluminator.PleasedoNOTerasethecameraSDcard.Wego

throughtheimagesandplatestoevaluateourprogram.Evenifyoudonotget“good”results,takeapictureforusso

wecanfigureoutwhatwentwrong.Pleasesaveasetofyourbestplatesand“weird”platesforme.PlacetheminaZiplocbagandbringthembacktothedistributioncenter.

BeforeRETURNINGthekit:*Pleaserepacktheequipmentinthecorrectboxes.Theboxesallhavepicturesoftheircontents.

*Besuretorinsetheelectrophoresisgelboxes,geltrays,gelcastingboardetc.withdistilledwaterandletthemAIRDRYONLYbeforepackingthem.Removeanytapeyoumayhaveputonthem.DONOTtrytowipethemdrywithapapertowelorethanol!

*Besuretopackallgelcombs,thermalcyclergrids,SDcards,cords,gelcastingboard,spatula,isofreezeunits,freezer

packsandemptyreagentbottlesastheseitemsaresometimesforgotten.

*IfanypiecesofequipmentdidnotworkpleaselabelthemandbringittoGrace’sattention.Youareultimatelyresponsibleforyourstudentsuseoftheequipment.Monitorthematalltimes.

*PLEASEREFILLALLTIPBOXESBEFORERETURNINGTHEKIT.

WHATGOESINTHEAUTOCLAVEBAG:ONLYUSEDMICROFUGETUBES,PIPETTIPS,AGARPLATES,ANDCELLSPREADERSFROMLABS5,6ANDColonyPCR,(TRANSFORMATION,PURIFICATIONANDPCRLABS)ARETOBEDISCARDEDINTHEAUTOCLAVEBAGSPROVIDED.USEATWISTTIETOCLOSETHEBAGANDONLYFILLEACHBAGUPTOTHEWORDS“AUTOCLAVEBAG”.

Used1XSBbuffercanbepoureddownthedrain.Allotherwaste(gloves,tubes,tips,etc)fromlabs1,2,3and4canbediscardedinyourregulartrash.

AnswerSurveysontheABE-LA.orgpageby…1)GotoABE-LA.org2)ClickonPASADENAatthetopofthepage.3)ScrolldowntothebottomofthepageandclickonBOTHOnlineDataCollectionANDOnlineResultsSurvey.4)Filloutbothformsandpresssubmit.YoumayhavetoanswerNOtosomeofthelabresultquestions.THANKYOU!!!

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Rev06_2015

MaterialsReturnandWasteDisposalProtocolforthe

PASADENAABESite

1. UsedtipsandtubesfromLabs1-4cangointoyourschoolwaste.Used1XSBmaybepoureddownthedrain.

2. Plates,tubes,tips,kimwipesandcellspreadersfromLabs5,6ANDColonyPCRgointheautoclavebagssuppliedbyABE-PASADENA.Bagsshouldonlybe2/3fullandtwisttiedclosed.NoOTHERTRASH.

a. Afteremptyingcontents,cups/beakersusedintheselabshouldbe

sprayedwith70%isopropylalcoholsolution,allowedtositfor15

minutes,drained,washedandreturned.Iftheteachersuppliedthese

cups,afterthe70%isopropylalcoholtreatment,theymaybe

disposedofintheregulartrash–donotputthemintheautoclavebags.Someplasticsproducetoxicfumeswhenautoclaved.

3. IfyoudidLab6,theflaskofremainingovernightcultureshouldbeplaced

inthelabeledzipbaggie,refrigerated,andreturnedtoABE-PASADENA.

4. Iftheplasmid,enzyme,andbufferreagenttubeshaveanythingleftin

them,pleasekeepthemrefrigeratedandreturntoABE-PASADENAinthe

icechestalongwithourisofreezeunitandicepacks.

5. Pleasereturnallotherunusedsuppliesand20XSBbottles.

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DirectionstoAmgenBiotechExperience–PasadenaDistributionCenterPickupandDropoffwithGraceMontgomery

2265 E Foothill Blvd, Pasadena, California 91107

ComingfromtheEast,onthe210:• GetoffattheSierraMadreBlvdexit.

• Makealeftatthelightattheendoftheramp.

• BesuretomakeawidelefttogetontotheSierraMadreBlvd.

• AttheItalianRestaurantandBistromakearight;youarenowonE.FoothillBlvd.

• Ourbuildingisinthesecondblockontherighthandsideofthestreet.

• Thedrivewayisbeforetheredbrickbuilding;itrunsalongtherightsideofthebuilding(thedarker

pavementunderthetreetotherightinthepicturebelow)asyoulookatitfromacrossthestreet.

• Iamlocatedbehindthethirddoorthatyousee,intheback(theonefarthestfromthedrivewaythatyoujustdrovedown).

• Call(626)696-9123andRINGTHEBELL,Iwillcomeandopenupthedoorforyou.

ComingfromtheWeston210:• GetoffattheSierraMadreBlvd./Altadenaexit.

• Makearightatthelightattheendoftheramp,thisisAltaDenaDr.

• Gotothefirstlightandmakearight,youarenowonSierraMadreBlvd.

• AttheItalianRestaurantandBistromakearight;youarenowonE.FoothillBlvd.

• Ourbuildingisinthesecondblockontherighthandsideofthestreet.

• Thedrivewayisbeforetheredbrickbuilding;itrunsalongtherightsideofthebuilding(thedarker

pavementunderthetreetotherightinthepicturebelow)asyoulookatitfromacrossthestreet.

• Iamlocatedbehindthethirddoorthatyousee,intheback(theonefarthestfromthedrivewaythatyoujustdrovedown).

• Call(626)696-9123andRINGTHEBELL,Iwillcomeandopenupthedoorforyou.

driveway

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AmgenBiotechExperience

KitCheckoutList-PASADENA

Teacher:ABEKit:

Datecheckedout:School:

Datecheckedin:BO

X1OUTIN

BOX2

OUT

INBO

X3OUT

IN1transillum

inatorw/cord

3powersuppliesw

/cords6gelcham

bersFotoDyne1hood/cam

erafordocumentation

13P-20pipettes6geltrays

1spatulaforgels13P-200pipettes

6-12toothedgelcombs

1cameracharger

13P-1000pipette1m

icrocentrifugew/cord

1SDcardreader1Pow

erstrip1m

inifuge1digitalbalance

BOX4

BOX5(Lab6only)

(Boxcontaining)1w

aterbath1shakingincubator

13tubesSol.113tuberacks

12columnstands&

binderclips13tubesSol.2

2boxesof12markers

12smallw

astebeakers13tubesSol.3

4waterbathm

icrotubeholdersrubberbands

1Vortexor12HICColum

nsw/tipsandlids

2Erlenmeyerflasks(1L&

1S)BO

X6(PCRonly)3M

ultisizedRacks1therm

ocyclerParafilmKitN

otebook(boxcontaining)

Digitalthermom

eter10P20-200tipboxes

NON-BO

XED6P1000tipboxes

1incubator1m

ulti-gelcastingtray(sm

allboxcontaining)Distilledw

atercarboy12m

inipracticegels13tubesreddye12pipettingtem

plates


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