participant 8 assistant contractor to participant 7 michael tausz, dieter grill karin herbinger,...
TRANSCRIPT
![Page 1: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/1.jpg)
Participant 8
Assistant Contractor to Participant 7
Michael Tausz, Dieter Grill
Karin Herbinger, Klaus Remele, Maria Müller
Institute of Plantphysiology, University of Graz, Schubertstraße 51, A-8010 Graz, Austria
![Page 2: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/2.jpg)
Investigations
• Biochemical defense– Ascorbate system– Glutathione system– Tocopherols– Chloroplast pigments
• Chromosomal damages– Aberration rates
![Page 3: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/3.jpg)
Experiments
Container exposed young beech trees
Branch cuvette exposures
Mature beech trees
sampling 2003
![Page 4: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/4.jpg)
Container exposed young (3-yrs-old) trees
exposed at 2 canopy levels: sun and shade
- Pigments and tocopherols
June, July, September
- Ascorbate and glutathione
due to insufficient material only September
Leaves:
Roots:- Ascorbate, glutathione and tocopherols
due to insufficient material only September
- Chromosomal aberration analysis
September
interface to partner 7
![Page 5: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/5.jpg)
Branch cuvette exposures
- Pigments, tocopherols, ascorbate and glutathione
September
in leave samples included in branch cuvette fumigation systems
interface to partner 7
![Page 6: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/6.jpg)
Mature trees
- tocopherols
May, June, July, September, Oktober
- Chromosomal aberration analysisMay, June, July, September, Oktober
interface to partner 4 who contributes ascorbate and glutathione
Leaves:
- Pigments and tocopherolsMay, June, July, September, Oktober
Roots:
![Page 7: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/7.jpg)
Chromosomal aberrations
- are approved indicators for genetic damages and
- could be defined as changes in the structure of chromosoms
![Page 8: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/8.jpg)
Chromosomal aberration analysis
Material: root tips of young and old beech trees
We adjusted the method
- in connection with the staining procedure (double staining: Schiff reagent, Giemsa, or Schiff reagent and carmine acetic acid)
- in connection with the identification of chromosomal aberrations in meta- and anaphases
(bridge, sticky chromosomes, vagrant, fragment etc)
![Page 9: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/9.jpg)
Aberration types- sticky chromosomes - anaphase bridges
- vagrant chromosomes anaphasemetaphase
![Page 10: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/10.jpg)
Problems:
The cell walls of most tips were lignified
and/or mycorrhized –
but:
enough root tips for the classification of
chromosomal aberrations
![Page 11: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/11.jpg)
viol
axan
thin
neox
anth
in
zeax
anth
in
anth
erax
anth
in
chlo
roph
yll a
-c
arot
ene
Pigments: Chromatogram
chlo
roph
yll b
lute
in
-ca
rote
ne
![Page 12: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/12.jpg)
0
2
4
6
8
10
12
14
A A
b
July 2003
young treesmature trees
SHADESHADE SUNSUN
total chlorophyll [mg g
-1 dry weight]
1 x ozone 2 x ozone
tota
l chl
orop
hyll
[mg
g-1 d
ry w
eigh
t]0
2
4
6
8
10
12
14
B
C
aa
b
sig. diff. mature/young:Sun 1 x ozoneSun 2 x ozoneShade 1 x ozone
Total chlorophyll
0
2
4
6
8
10
12
14
AAbc
June 2003
young treesmature trees
SHADESHADE SUNSUN
total chlorophyll [mg g
-1 dry weight]
1 x ozone 2 x ozone
tota
l chl
orop
hyll
[mg
g-1 d
ry w
eigh
t]
0
2
4
6
8
10
12
14B
B
a
cab
sig. diff. mature/young:Shade 1 x ozoneShade 2 x ozone
![Page 13: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/13.jpg)
0
2
4
6
8
10
12
14
Mai
Juni
Juli
OktoberSeptemberJulyJuneMay
0
2
4
6
8
10
12
14
16
Juni Ju
li
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
tota
l chl
orop
hyll
[mg
g-1 d
ry w
eig
ht]
Total chlorophyll
?Containers removed
![Page 14: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/14.jpg)
-Carotene
0
50
100
150
200
250
300
350
400
450
sig. diff. mature/young:Sun 1 x ozoneSun 2 x ozoneShade 1 x ozoneShade 2 x ozone
AB
AA
c
July 2003
young treesmature trees
SHADESHADE SUNSUN
-carotene [µ
g g-1 dry w
eight]
1 x ozone 2 x ozone
-ca
rote
ne [µ
g g-1
dry
wei
ght]
0
50
100
150
200
250
300
350
400
450
ab
bc
a
0
50
100
150
200
250
300
350
400
450
sig. diff. mature/young:Sun 1 x ozoneSun 2 x ozone
C
A
B
A
c
June 2003
young treesmature trees
SHADESHADE SUNSUN
-carotene [µ
g g-1 dry w
eight]
1 x ozone 2 x ozone
-ca
rote
ne [µ
g g-1
dry
wei
ght]
0
50
100
150
200
250
300
350
400
450
a
b
a
![Page 15: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/15.jpg)
0
50
100
150
200
250
300
350
Mai Ju
ni
Juli
OktoberSeptemberJulyJuneMay
0
50
100
150
200
250
300
350
400
Juni
Juli
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
-ca
rote
ne [
µg
g-1 d
ry w
eig
ht]
-Carotene
Containers removed
![Page 16: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/16.jpg)
-Carotene
0
50
100
150
200
250
300
350
400
450
AA
B
AB
b
a aab
July 2003
young treesmature trees
SHADESHADE SUNSUN
-carotene [µg g
-1 dry weight]
1 x ozone 2 x ozone
-ca
rote
ne [µ
g g-1
dry
wei
ght]
0
50
100
150
200
250
300
350
400
450
sig. diff. mature/youngSun 1 x ozoneSun 2 x ozoneShade 1 x ozoneShade 2 x ozone
0
50
100
150
200
250
300
350
400
450
b
aab
ab
ba abab
June 2003
young treesmature trees
SHADESHADE SUNSUN
-carotene [µg g
-1 dry weight]
1 x ozone 2 x ozone
-ca
rote
ne [µ
g g-1
dry
wei
ght]
0
50
100
150
200
250
300
350
400
450
no sig. diff. mature/young
![Page 17: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/17.jpg)
050
100150200250300350400450
Mai
Juni
Juli
OktoberSeptemberJulyJuneMay
050
100150200250300350400450500
Juni
Juli
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
-ca
rote
ne [
µg
g-1 d
ry w
eig
ht]
-Carotene
Containers removed
![Page 18: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/18.jpg)
0
100
200
300
400
500
Mai
Juni
Juli
OktoberSeptemberJulyJuneMay
0
100
200
300
400
500
600
Juni
Juli
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
neox
anth
in [
µg
g-1 d
ry w
eig
ht]
Neoxanthin
Containers removed
![Page 19: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/19.jpg)
0100200300400500600700800900
1000
Mai
Juni
Juli
OktoberSeptemberJulyJuneMay
0100200300400500600700800900
10001100
Juni
Juli
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
lute
in [
µg
g-1 d
ry w
eig
ht]
Lutein
Containers removed
![Page 20: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/20.jpg)
Xanthophyll cycle
Zeaxanthin
Antheraxanthin
Violaxanthin
Light-protection
Light-harvesting
De-epoxidised
Epoxidised
Protection from excessive light energy
![Page 21: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/21.jpg)
Violaxanthin + Antheraxanthin + Zeaxanthin
0
50
100
150
200
250
300
350
400
450
500
550July 2003
young treesmature trees
SHADESHADE SUNSUN
V+
A+
Z [µ
g g-1 dry w
eight]
1 x ozone 2 x ozone
V+
A+
Z [µ
g g-1
dry
wei
ght]
0
50
100
150
200
250
300
350
400
450
500
550
AA
A
Aaa
aa
sig. diff. mature/young:Sun 1 x ozoneShade 1 x ozoneShade 2 x ozone
0
50
100
150
200
250
300
350
400
450
500
550June 2003
young treesmature trees
SHADESHADE SUNSUN
V+
A+
Z [µ
g g-1 dry w
eight]
1 x ozone 2 x ozone
V+
A+
Z [µ
g g-1
dry
wei
ght]
0
50
100
150
200
250
300
350
400
450
500
550
B
BB
Aaaaa
sig. diff. mature/young:Shade 1 x ozoneShade 2 x ozone
![Page 22: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/22.jpg)
0
50100150200250300350400450
Mai
Juni
Juli
OktoberSeptemberJulyJuneMay
050
100150200250300350400450500
Juni
Juli
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
V+
A+
Z [
µg
g-1 d
ry w
eig
ht]
Violaxanthin + Antheraxanthin + Zeaxanthin
Containers removed
![Page 23: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/23.jpg)
Deepoxidstate
0.0
0.2
0.4
0.6
0.8
1.0July 2003
young treesmature trees
SHADESHADE SUNSUN
Deepoxidstate
1 x ozone 2 x ozone
Dee
poxi
dsta
te
0.0
0.2
0.4
0.6
0.8
1.0
no sig. diff. mature/young:
BB
A A
bb
aba
0.0
0.2
0.4
0.6
0.8
1.0June 2003
young treesmature trees
SHADESHADE SUNSUN
Deepoxidstate
1 x ozone 2 x ozone
Dee
poxi
dsta
te
0.0
0.2
0.4
0.6
0.8
1.0
sig. diff. mature/young: Sun 2 x ozone
BB
AA
c
bab
a
![Page 24: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/24.jpg)
0.0
0.2
0.4
0.6
0.8
Mai
Juni Ju
li
OktoberSeptemberJulyJuneMay
0.0
0.2
0.4
0.6
0.8
1.0
Juni
Juli
young trees
mature trees
September OktoberJulyJuneMay
Sun 1 x ozone Sun 2 x ozone Sha 1 x ozone Sha 2 x ozone
Dee
poxi
dsta
te
Deepoxidstate
Containers removed
![Page 25: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/25.jpg)
0
100
200
300
400
500
600
700
800
900
1000
sig. diff. mature/young:Sun 2 x ozone
BB
AA
a
bb
a
June 2003
young treesmature trees
SHADESHADE SUNSUN
-tocopherol [µ
g g-1 dry w
eight]
1 x ozone 2 x ozone
-to
coph
erol
[µg
g-1 d
ry w
eigh
t]
0
100
200
300
400
500
600
700
800
900
1000
2222
-Tocopherol
![Page 26: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/26.jpg)
First results on pigments and -tocopherol show differences between sun and shade crown.
Shade leaves exhibited higher pigment content and lower deepoxidate state of xanthophyll cyclus.
Only marginal differences were found between ozone treatments.
Summerised
![Page 27: Participant 8 Assistant Contractor to Participant 7 Michael Tausz, Dieter Grill Karin Herbinger, Klaus Remele, Maria Müller Institute of Plantphysiology,](https://reader036.vdocuments.us/reader036/viewer/2022081501/5697bfb51a28abf838c9d51a/html5/thumbnails/27.jpg)
Outlook
for the next months
Investigations of
- pigments and tocopherol (remaining sampling dates)
- Ascorbate and gluthatione (in young trees, branch cuvetts)
- Chromosomal abberations
statistical evaluation
comparison with the results of other participants
and interpretation