paper 3 topical exercises

7/28/2019 Paper 3 Topical Exercises

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EXERCISE 1

Figure 1 shows the apparatus set up to investigate the effect of temperature on the reaction ofamylase.

DIAGRAM 1

After 5 minutes of incubation , saliva in test tubes L, N, Q and S are poured into test tubes K, M,P and R respectively. The content of each test tube then are tested to check the presence ofstarch using iodine solution for every one minute. The student labeled the white cavity tile asbelow:

1

1 2 3 4 5

6 7 8 9 10

Blue black

Brown solution

minutes

minutes


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Table 1 shows the Iodine test result in test tubes K, M, P and R.

Tube Temperature(0C)

Iodine test resultTime taken forhydrolysis of

starch (minutes)

K 0Not completedafter 10 minutes(0 minutes)

M 20

P 37

R 60

TABLE 1.1

(a) Based on Table 1, state twoobservations from the experiment that can be made.

1. ..........................................................................................................................

..........................................................................................................................

2. ..........................................................................................................................

..........................................................................................................................

(b) State the inference which corresponds to the observation in (b) (i) above.

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1. ............................................................................................................

.........................................................................................................................................

.............................

2.

...........................................................................................................................

.............................

(c) Record the time taken for hydrolysis of starch to be completed in Table 1.

(d) Complete Table 1.2 based on this experiment

Variable Method to handle the variable

Manipulated variable

.

.

..

..

Responding variable

..

.......

Controlled variable

.

.

.

(e) State the hypothesis for this experiment.

.............................................................

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(f) Based on table 1.1 construct a table and record the results of the experiment whichincludes the following aspects:

o Temperature

o Time taken for hydrolysis of starch

o Rate of amylase reaction

(g) On the graph paperdraw the graph of rate of amylase reaction against the temperaturefrom the results in (e)(i).

(h) Explain the relationship between temperature and the rate of enzyme activity.

..............

..............

.............

(i) The experiment is repeated by increasing the concentration of saliva. Predict the time

taken for a complete hydrolysis of starch at 370C.

......

.

.................................................

............................

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EXERCISE 2

An experiment is carried out to investigate the effect of certain factor on the activity of yeast.

Yeast reacts with sucrose solution to produce alcohol and carbon dioxide gas.

First the apparatus as shown in Figure 1 is set up. 1cm 3 of 1% yeast suspension is transferredusing a pipette into a boiling tube P containing 5 cm3 of 1% sucrose solution. The initial level ofthe coloured liquid in the U tube is set at 0.0 mm. After exactly 10 minutes, the level of thecoloured liquid is as shown in the diagram.

Diagram 1

The same experiment is repeated using boiling tubes Q, R and S, with the same volume ofsucrose solution of 2%, 3% and 4% concentration respectively measured using a pipette. Thechanges of the coloured liquid levels in the U-tube are shown in Figure 2.

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Level of coloured liquid

Boiling tube P (1%) Boiling tube Q (2%) Boiling tube R (3%) Boiling tube S (4%)

25.0 mm

[ 3 marks]Diagram 2

(a) Record the levels of the coloured liquid in the U-tubes in the spaces provided inDiagram 2.

(b) Based on the results in Figure 2, state twoobservations that can be made for theexperiment.

1.

2..

..

(c) Write down an inference foreach of the observations in (a).

1..

..

2......

.

.

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(d) Complete Table 3 based on this experiment



.

.

.

Responding variable

.

Controlled variable

.

.

.

Table 3

(e) State the hypothesis for the experiment ?

...

...

(f) If the sucrose solution concentration is increased to 6%, predict the rate of carbon

dioxide produced by the yeast in 10 minutes.

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g (i) The volume of the carbon dioxide produced by the yeast in the boiling tube can becalculated using the following formula:

The volume of the carbon dioxide produced (ml) = The cross-section of the glass tube

(mm

2

) X the distance traveled by the coloured liquid (mm)

The volume of carbon dioxide produced (mm3)The rate of carbon dioxide produced = ---------------------------------------------------------------------

Time taken for the coloured liquid to travel (s)

Based on Figure 2, record the results of this experiment obtained in (c) in a table, giventhat the cross section of the glass tube is 3 mm 2. Your table should include the followingaspects:

- The concentration of the sucrose solution- The distance traveled by the coloured liquid

- The rate of carbon dioxide produced

(ii) From the table in (f) (i) above, draw a graph of the concentration of the sucrose solutionagainst the rate of carbon dioxide produced.

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(h) From the table you have drawn in (f) above, explain the relationship between the

concentration of sucrose solution, the height of the coloured liquid and the rate of carbon

dioxide produced by the yeast.

(i) From the experiment, what can be deduced about fermentation of yeast ?

(j) The following list of apparatus and materials are used in this experiment.

Pipette 1% yeast solution scale coloured liquid 1% to 4% sucrose solution.

Complete the table 1.4 below by matching each variables with the materials andapparatus used in this experiment

Variables Apparatus Materials

Manipulated

Responding

Constant

Table 1.4

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EXERCISE 3

A group of students carried out an experiment to investigate the effect of running up and down astair case on the rate of heart beat of a person. Figure 1.1 shows the method used by thestudents. The subject runs up the stair case and straight away runs down the same stair case.The time taken for the boy to run up and down the stair case is approximately 5 seconds.

Immediately after the boy has finished running up and down the stair case, the time taken for 30heart beats is taken and is recorded in Figure 1.1.

The whole experiment was repeated with the same boy running up the stair case as shown inFigure 1.2(a), 1.2(b) and 1.2(c) in Table 1.1 respectively. The results are also shown in thesame table.

Table 1.1Situation Numbers of times

the boy running

up and down thestair case.

Observation The time takenfor making 30

heart beats (s)

Figure 1,1

One time

20

Figure 1.2(a)

Two times

Figure 1.2(b)

Three times

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Figure 1.2(c)

Four times

a. (i) From Table 1.1, state twoobservations on the relationship of the numbers of times the

boy runs up and down the stair case and the time taken for 20 heart beats.

Observation 1:

..

Observation 2:

..

(ii) State the inference which corresponds to the observations in a (i).

Inference 1:

.

Inference 2:

.

b. Record the time taken for the boys heart to make 30 heart beats in the three occasions inTable 1.1.

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c. Complete Table 1.2 based on this experiment



.........

..

.

........

Responding variable

.........

..

.........

Fixed variable

.........

..

.

........

Table 1.2 (3 marks)

d. State the hypothesis for this experiment.

..

e. Predict the rate of the boys heart beat If he runs up and down the same stair case 5times.

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f. Based on Table 1.1, construct a table and record the results of the experiment which

include the following aspects:

- The numbers of time of running up and down the stair case

- The time taken for the boys heart to make 30 beats

- The rate of the boys heart beat (times/minute)

g. From the table in (e) (i) above, draw the graph of the rate of the boys heart beats against

the number of times of running up and down the stair case.

h. From the graph you have drawn in (e) above, explain the relationship between the number

of times of running up and down the stair case, the time taken for the boys heart to beat

30 times and the rate of the boys heart beat.

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EXERCISE 4

An experiment is carried out to investigate the pollution level of Kelantan river in three differentlocation. This is done due to complaints from the residents of three villages regarding the qualityof river water lately.

Three villages which residents made the above complaints are marked village P, village Q andvillage R. There is a rubber processing factory at the up stream of the river. The distancebetween the villages and the factory are 5km, 10km and 18km respectively. Figure 1.1 showsthe location of the situation.

Figure 1.1

The Biological Department of SMK Ismail Petra has conducted the investigation. Three samplesof water are collected from the three villages respectively, and named water sample P, watersample Q and water sample R. The samples are brought to the school lab and tests areconducted.

First, 50ml of the water sample P is measured using a measuring cylinder and poured into aclean reagent bottle. 1ml of 0.1% methylene blue solution is added into the water sample usinga syringe. The mixture is stirred carefully with the needle of the syringe. The reagent bottle isclosed quickly and kept in a dark cupboard (Figure 1.2). The stop watch is started (Table 1.1)and the colour of the solution is observed from time to time to determine the time taken for the0.1% methylene blue solution to decolourise.

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Figure 1.2

The above steps are repeated with water sample Q and water sample R. The time taken for thesolution to decolourise is recorded in table 1.1.

Watersampletakenfrom

village

Treatment

Time shown on the stopwatch when the

methylene blue solutiondecolourises

Timeelapsed

(min)

P

Q

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R

Table 1.1

a. Based on the results in Table 1.1, state twoobservations for the experiment.

1

.

2

.

b. Write down an inference foreach of the observations in (a).

1

...

2

..

c. Record the time taken for the decolourisation of 0.1% methylene blue solution in the spaces

provided in Table 1.1.

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d. Complete Table 2.1 based on this experiment



...

Responding variable

...

Controlled variable

..

Table 2.1

e. State the hypothesis for the experiment

..

..

f. If a village, D, is situated 14km from the rubber factory, predict the level of B.O.D. of this

village.

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g. Based on Table 1.1, record the results of this experiment obtained in (c) in a table. Yourtable should include the following aspects:

o The water sample

o The distance between the village and the rubber factory

o The time taken for the 0.1% methylene blue to decolouriseo The BOD level of the water samples (high, moderate and low)

i. From the table in (f) (i) above, draw a graph of time for 0.1% methylene blue solution todecolourise against the distance between the village and the rubber factory.

j. From the table you have drawn in (f) above, explain the relationship between the distance

between the rubber factory, the time taken for the decolourisation of 0.1% methylene

blue solution and the B.O.D. level of the water sample.

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EXERCISE 5

Water is very important to living organisms. Its roles in an organism include helping in

transportation of substances in the body, acts as a solvent for most of the substances in thebody and helps to carry out homeostatic regulations in the body.

In man, water is taken in through drinking. Some of the water is expelled through urination.The amount of urine expelled varies from a situation to another.

Halim carries out an experiment to determine the effects the volume of water intake by aman to the volume of urine formation. First he empties his urinary bladder. Then he drinks200ml of boiled water. He stays in the biology laboratory for 30 minutes and then goes tourinate in the toilet. The urine is kept in the lab. After another 30 minutes, he goes to urinateagain. The volumes of the two urination are added. The whole experiment is repeated,replacing the water intake volume by 300ml, 400ml and 500ml respectively and assuming

Halim does not take any food during the experiment.

The results of the experiment are tabulated as in table 1.1.

Intake ofwater (ml)

First urine collection(cm3)

Second urine collection(cm3)

Total urine collection(cm3)

200 90

300

400

500

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Table 1.1

a. Based on the results in Figure 2, state twoobservations for the experiment.

1

2

b. Write down an inference foreach of the observations in (a).

1

2.

c. Record the levels of the urine collected in the measuring cylinder in the spaces providedin Table 1.1

d. Complete Table 2 based on this experiment



..

Responding variable

..

Fixed variable

..

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paper 3 topical exercises

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