p53- and drug-induced apoptotic responses mediated by bh3-only proteins puma and noxa p53- and...
TRANSCRIPT
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P53- and Drug-Induced P53- and Drug-Induced Apoptotic Responses Mediated Apoptotic Responses Mediated by BH3-Only Proteins Puma by BH3-Only Proteins Puma
and Noxaand Noxa
Villunger, A. et al. Science, 2003
Flávia Gama Gomes Leite
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p53 – ciclo celular e p53 – ciclo celular e apoptoseapoptose
p53
p21
Parada do ciclo celular
Mais de 16 genes alvos
Apoptose ?
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Possíveis Genes AlvosPossíveis Genes Alvos
Puma (p53-upregulated modulator of apoptosis)
Noxa (Noxius stress)
Domínio BH3
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Construção de NoxaConstrução de Noxa-/--/-
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NoxaNoxa-/--/-
Northern blot
Agarose - controle
Southern blot
PCR
Southern blot
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NoxaNoxa-/--/-
Estímulo dependente de p53
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NoxaNoxa-/--/-
Estímulos independentes de p53
Estímulos dependentes de p53
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Construção de PumaConstrução de Puma-/--/-
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PumaPuma-/--/-Southern blot
PCR
Southern blot - sonda exon 2
RT- PCR
Northen blot
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PumaPuma-/--/-
genotóxico
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Independente de p53
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PumaPuma-/--/-
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Puma-/-