p. pőthe 1 , i. gergely 1 , v.Ördög 1,2
DESCRIPTION
Effect of microalgal biomass from MACC-612 Nostoc enthophytum and MACC-430 Tetracystis sp. on sunflower production. P. Pőthe 1 , I. Gergely 1 , V.Ördög 1,2. 1 University of West Hungary, Faculty of Agricultural and Food Sciences, - PowerPoint PPT PresentationTRANSCRIPT
Effect of microalgal biomass from MACC-612 Nostoc enthophytum and MACC-430 Tetracystis sp. on sunflower
production
P. Pőthe1, I. Gergely1, V.Ördög1,2
1University of West Hungary, Faculty of Agricultural and Food Sciences, Institute of Plant Biology.9200 Mosonmagyaróvár, Kolbai K. Str 8., Hungary
2Research Centre for Plant Growth and Development, University of KwaZulu-Natal,Pietermaritzburg, P/BAG X01, Scottsville 3209, South Africa
International Scientific Conference for PhD Students the University of West Hungarythe University of West Hungary Győr,Hungary, March 19-20,2013
Talentum Project
The background of experiments
• Hormon test of microalgae strains in Mosonmagyaróvár (1990-)
• Selection of fast-growing algae strains
• Investigation of biological effects of selected microalgae strains on sunflower
Plant hormone production of MACC strains
BioassaysBioassays for detection of for detection of auxin- and cytokinin-like activityauxin- and cytokinin-like activity
Auxin-like activityAuxin-like activity: Cucumber cotyledon rooting : Cucumber cotyledon rooting bioassay bioassay (I(IBABA, , indole-butyric acidindole-butyric acid)),, ((Zhao et al 1992Zhao et al 1992))Cytikonin like activityCytikonin like activity: : Cucumber cotyledon growth cotyledon growth bioassay bioassay (KIN, kinetin(KIN, kinetinee)), (, (Zhao et al 1992Zhao et al 1992))
151,37
138,48
136,79
121,17
100 99,26
106,2
0
20
40
60
80
100
120
140
160
0 0,3 0,38 0,5 1 3 10
kinetine concentration (mg/l)
Fre
sh w
eig
ht
of
10 c
oty
led
on
s in
% o
f co
ntr
ol
Cytokinin-like activity of the MACC-612 biomass in 2 g/L conc.Cytokinin-like activity of the MACC-612 biomass in 2 g/L conc.
168,85
112,1
100
79,44
136,48
165,8
142142
0
20
40
60
80
100
120
140
160
180
0 0,25 0,3 0,5 0,96 1 3 10
IBA concentration (mg/l)
Ro
ot
nu
mb
er in
% o
f co
ntr
ol
Auxin-like activity of the MACC-612 biomass in 2 g/L conc.Auxin-like activity of the MACC-612 biomass in 2 g/L conc.
TREATMENT OF SUNFLOWER WITH HORMONE-TREATMENT OF SUNFLOWER WITH HORMONE-PRODUCING MICROALGAEPRODUCING MICROALGAE
1.1. MACC-6MACC-61212, , cyanobacterium cyanobacterium strain with strain with cytokinincytokinin--like activity,like activity,
2.2. MMACC-ACC-430430, microalga strain with auxin- , microalga strain with auxin- and and cytokinin like cytokinin like activityactivity
Nostoc entophytum MACC-612
Algae biomass production
• Cultivation in Zehnder Z-8 (MACC-612)
• Centrifugation and freeze-drying
• Freeze-dried biomass
Experiment
• Place: Mosonmagyaróvár ( Hungary)• Sowing:04.04.2012• Plot size: 27 m2
• Number of Treatments: 7• Number of Repetitions: 4• Treatments:
» 4-6 leafy (Phenophase I.)» Rosette growth (Phenophase II.)
• Harvesting: 17.09.2012• Observations and measurements of the treatments were
between May and October of 2012
Spraying at phenophase I. (4-6 leafy)
Spraying at phenophase II.(rosette growth)
TreatmentsTreatments Dose
g/haVolume of the spray (l/ha)
Phenophase
BBCH-scale
Date of treatment
I. Control - - -
II. MACC-612 400 400 4-6 leafy BBCH-12 19.05.2012
III. MACC-430 400 400 4-6 leafy BBCH-12 19.05.2012
IV. MACC-612 700 700 rosette growth BBCH-18/32 23.06.2012
V. MACC-430 700 700 rosette growth BBCH-18/32 23.06.2012
VI.MACC-612
400 400 4-6 leafy BBCH-12 19.05.2012
700 700 rosette growth BBCH18/32 23.06.2012
VII.MACC-430
400 400 4-6 leafy BBCH-12 19.05.2012
700 700 rosette growth BBCH18/32 23.06.2012
Investigated parameters I.
On field:
• Plant height• Plate diameter• Root mass• Yield• Phytotoxicity
Investigated parameters II.
In laboratory:
• Gross plate weight (g)• Net plate weight (g)• 1000 achene weight (g)• Oil content• Oil yield (kg/ha)
Plant height of sunflower(cm)
150 151 153 154,5 155 158
157
150
Plate diameter at harvest
I. ControllII. MACC-612 1x sprayingIII. MACC-430 1x sprayingIV. MACC-612 1x sprayingV. MACC-430 1x sprayingVI. MACC-612 2x sprayingVII.MACC-430 2x spraying
Root mass at harvest time
I. ControllII. MACC-612 1x sprayingIII. MACC-430 1x sprayingIV. MACC-612 1x sprayingV. MACC-430 1x sprayingVI. MACC-612 2x sprayingVII.MACC-430 2x spraying
Statistically significant results
Sunflower yield
I. ControllII. MACC-612 1x sprayingIII. MACC-430 1x sprayingIV. MACC-612 1x sprayingV. MACC-430 1x sprayingVI. MACC-612 2x sprayingVII.MACC-430 2x spraying
2621,3* 2603,3*2551,5*
*P < 0,05%
Gross Plate Weight
I. ControllII. MACC-612 1x sprayingIII. MACC-430 1x sprayingIV. MACC-612 1x sprayingV. MACC-430 1x sprayingVI. MACC-612 2x sprayingVII.MACC-430 2x spraying
86 93 92
107,6*
105,4
116,4* 112,4*
*P < 0,05%
Thousand Achene Weight
Treatments
68,24* 67,38*
*P < 0,05%
Oil Content (%)
Oil Yield (kg/ha)
Mosonmagyaróvár (497mm)
The geographic distribution of precipitation in Hungary in 2012
Deviation of average monthly precipitation of 2012 from the many year's (1981-2011) values in Hungary
Conclusion and suggestions
• The treatment of sunflower with 0,1% Nostoc enthophytum biomass positively affected the growth, development and consequently the yield of sunflower
• The treatments did not cause phytotoxicity
• Based on the presented results it can be stated that plant treatment with selected microalgae strains is a good tool for overcomming the drought stress in sunflower
Thank you for your attention!Thank you for your attention!