BIOLOGICAL OXIDATION – 3

▪ OXIDATIVE PHOSPHORYLATION ▪ INHIBITORS ▪ UNCOUPLERS

RITTU CHANDEL19-03-2013

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OVERVIEW

CARBOHYDRATES

FATSAMINO ACIDS

CELLULAR RESPIRATION

ENERGY OF OXIDATION DRIVES THE SYNTHESIS OF ATP

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Biochemical anatomy of mitochondria

POWER HOUSE 0f cell

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OXIDATIVE PHOSPHORYLATION

Transport of electron through electron transport chain is linked with release of free energy

Process of synthesizing ATP from ADP and Pi coupled with ETC

Site : complex V of inner mitochondrial membrane

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SUMMARY:ELECTRON TRANSPORT CHAIN

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ATP

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TRANSFER OF ELECTRON FROM NADH THROUGH ETC

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PROTON MOTIVE FORCE

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CHEMIOSMOTOIC THEORY

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COUPLING OF ELECTRON TRANSFER AND ATP SYNTHESIS

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FoF1 ATPase or ATP synthase or Complex V

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ROTATORY OR ENGINE DRIVING MODEL

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TOP VIEW OF F1

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BINDING CHANGE MECHANISM-PAUL BOYER

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ATP ADP TRANSLOCASE

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MECHANISM OF ATP - ADP TRANSLOCASE

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P/O RATIO

Number of molecules of ATP synthesized per pair of electron carried through ETC

P/O for oxidation of NADH = = 2.5

P/O for oxidation of FADH2 = 1.5

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REGULATION OF OXIDATIVE PHOSPHORYLATION

Availability of ADP regulates the process

If ATP is low, ADP is high it proceeds a rapid rate

TIOXYGEN UPTAKE

TIMErest

ADP increased

Returning to rest

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UNCOUPLERS

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UNCOUPLER

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UNCOUPLERS

PHYSIOLOGICALThyroxine in high dosesThermogenin or UCP 1Unconjugated hyperbilirubinemia

SYNTHETIC2,4 – dinitrophenol2,4 – dinitrocresolFCCP, Gramicidin A,high doses of aspirin

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INHIBITORS

Oligomycin,Dicyclohexylcarbodiimide aurovertin4-chloro – 7- nitrobenzofurazan Atractyloside bongregate

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summary

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CHEMICAL COUPLING

By E.Slater A high energy phosphorylated

intermediate is common to both ETC and phosphorylation of ADP

Why was this not accepted ?

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SUBSTRATE LEVEL PHOSPHORYLATION

In glycolysis

In TCA cycleConversion of succinyl CoA to succinic

acid

Lohman reaction Myokinase reaction

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OXIDATIVE PHOSPHORYLATION DISEASES

EYEOphtalmople

giaretinopathy

LIVER

KIDNEYglomerulopat

hy

PANCREASDiabetes mellitus

INNER EARSensorineura

l loss

BRAINSeizures,atax

ia,stroke

SKELETAL MUSCLE

Myopathies

HEARTConduction disorders

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BIBLIOGRAPHY

SATYANARYAN VASUDEVAN PANKAJA NAIK LIPPINCOTT BHAGVAN MARKS GRISHAM LEHNINGER STRYER VOET HARPER

THANK YOU

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