osmosis in red blood cell (2ndrevision)

8/13/2019 Osmosis in Red Blood Cell (2ndrevision)

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Osmosis in red blood cell

Group 4

Algenio. Bresnan. Castro. Chang. Tan


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I. Abstract

This study aims to find the effect of osmosisin the cell. The researchers aim to answer thefollowing questions:

1. What are the different effects ofdifferent kinds of solution to the cell?

2. How does osmosis works?

The researchers used red blood cells andNaCl solutions with varying levels of

concentration. The set-ups were observed for anhour and produces different results.

It is later found out that the manipulation ofconcentration levels of the solution affects

osmosis in red blood cells.


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II. Introduction

As the basic unit of life, a cellhas its ownway to self-perpetuate. This may range fromcell division, cellular respiration, up to thetransport of different materials needed forthese processes. One example of such

process is osmosis which occurs inside of thecell. Osmosis is the net movement of a solvent,usually water, through a semi-permeablemembrane.

In this experiment, red blood cells wereused to observe this process of osmosis. Thisis made possible by manipulating theconcentration levels of the solution containing

these RBCs.


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III. Objectives

1. To distinguish the effects of a hypotonic,

hypertonic, and isotonic solution to the cell.

2. To determine how osmosis works.


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IV. Methodology

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1. Extract blood from a volunteer.

Three drops should be placed in

each glass slides labelled A, B,

and C.

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2.Add a drop of 0.07M, 0.15M,

and 0.30M NaCl solution

respectively to each glass

slides.

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3. The red blood cells will beobserved in an hour having five-minute intervals for measuringthe size.

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V. Results and

Discussion


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SAMPLE A0.07 M NaCl Solution

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B & C


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SAMPLE B0.15 M NaCl Solution

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B 0:30


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B. 1:00


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SAMPLE C0.30 M NaCl Solution

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C 0:30


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C 1:00


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Slide A contains a 0.07M NaCl solution.After a 1-hour mark, the cells notably grew

larger.

In slide B, the 0.15M solution does not

cause any changes to the size of the cells.

However in slide C, the 0.30M solution

causes the cells to shrink.


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VI. Conclusion

Since the 0.15 NaCl solution does not cause

any change to the size of the cells,it could be

said that osmosis doesn't takes place.

It can be also concluded that the concentration

inside the red blood cell is somehow near the0.15M margin. This makes the 0.15M NaCl

solution an isotonic one.

With these facts the 0.07M and 0.30M NaClsolutions can be called hypotonic andhypertonic solutions respectively. Thedifference in concentration levels of the cell andthe solution triggers the osmosis process as anattem t of the cell to achieve e uilibrium.


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The molar concentration of the solution isinversely proportional to the size of the red blood

cells. In other words, as you increase the

concentration of the solution, the cells size will

decrease.

Hypertonic solution (high c.) cell shrinks

Isotonic solution no changes in the cell

Hypotonic solution (low c.) cell enlargesand may burst


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-END-

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osmosis in red blood cell (2ndrevision)

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