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Oscar R. Miranda, PhD Address SENIOR BIOMEDICAL & PHARMACEUTICAL ENGINEER Massachusetts Institute of Technology (MIT) ormirandas@gmail.com 77 Massachusetts Avenue, Cambridge, MA 02139 http://web.mit.edu/nelman/www/page3/index.html

(US Permanent Resident) _______________________________________________________________________________ Summary • Innovative and highly-dedicated to passion for research and development of bio-sensing, drug formulation and platforms solutions through cellular manipulation and therapy in providing early disease detection for ongoing prevention treatment against fatal diseases and illnesses. • 15+ years of experience research sciences and developments, dedicated last 10 years of scientific contributions to micro/nano therapeutics, bio-sensing, investigate and formulate self-emulsifying drug delivery systems. • Written grants proposals, protocol for mice models, patent applications, review articles, and research articles on a variety of topics. • Extensive experience in teaching: Managed +1000 students from various disciplines of engineering, science and biomedical engineering as a teaching assistant. • Extensive hands-on experience with bio/nanomaterial synthesis and surface modification, bio-conjugation, drug delivery platforms, cell culture, engineering cells surface, DNA/aptamers, peptide synthesis, protein modification, circle rolling amplification, cell surface up taking, 3-D printer, solid work package design, organic synthesis, nanoparticles, biopolymer particles, hydrogels, liposomes/alginate systems, optoelectronic assay, immune and biological assays, micro-fluidic systems, bio-sensing, confocal microscopy, microtome, analytical characterization, protein/biochemical/cell-based assay develop, animal protocols, animal models, etc. • Possess strong record of publications in high impact journals such as Nature Nanotech., Nature Chemistry, Nature Protocols, Angew. Chem., Nano Letters, JACS, PNAS, ACS Nano, Nanomedicine, PLoS One, Adv. Mater., etc. (44+), and conference/Invited presentations (26+). • Inventor and medical solving problems (2+ Patents). • Recipient of several prestigious awards and grant (8) • Being honored to be safety officer on different Alma Mater laboratories including Villanova, UMass-Amherst, Harvard, BWH and MIT. • Proven capability in leading a project, managing multiple projects simultaneously, ability to lead multifunctional project teams, ability to drive for continuous-process improvement, work effectively in a multi-tasking environment, working well both in groups and independently, dedicated and attentive to successfully endeavor any assigned task, motivated self-starter with a "can-do" attitude, resourceful individual who maintains a positive, proactive attitude when faced with adversity, and have access to a network of experts in different fields including Medicine, Health Care, Science, Technology, Engineering, and Industry. _________________________________________________________________________________________________ Research of Interest

Main interest: Drug Delivery, Biomaterials, Cell Therapy, Bio-Medical Engineering, Translational Medicine Areas of Interest: Arthritis, Diabetes, Cancer (e.g. Lung Cancer, Neuroblastoma, Breast Cancer, Prostate Cancer), Regenerative Medicine, Surface Engineering, Cell Interactions, Cell Microenvironments, Diagnostics, Biosensors, Imaging, Biomarkers, Aptamers, Micro- and Nano-technology, Bio-conjugation, Treatment of Diseases. Objective

• To develop and use molecular and bioengineering tools with emphasis on small materials and bio-inspired biology in order to further understand, diagnose, image, and treat diseases to tackle unmet challenge in medical needs including but not limited to Inflammatory Arthritis, Diabetes and Cancer Subtypes.

• To work in the interface between chemistry, material science and engineering, and biology in collaboration with an interdisciplinary team including clinicians, pharmacologists, medical doctors and bioengineers to a) develop the next generation of macro/nano drug delivery systems to deliver drugs to patients in a safe, effective, and compliant manner; b) study biological, therapeutic, and detrimental functions of cell-based systems to better

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understand and utilize them to treat a variety of diseases; and c) synthesize, develop and engineer biomaterials for therapeutic applications in biomedicine.

Education

Post-Doctoral Associate in Biomedical Devices & Translational Research, with Dr. Noel Elman 03/2014-Present Massachusetts Institute of Technology (MIT) Institute for Soldier Nanotechnologies Cambridge, Massachusetts, USA.

• Developing, designing (e.g. Solid work software), re-engineering, and 3D printing biomedical devices prototypes for rapid microfluidic reconstitution of lyophilized drugs such as vaccines, antibiotics, and emergency medications for controlled release in rural areas, ambulatory setting, ERs, and disaster zones. This rapid reconstitution technology is for dramatically advancing soldier protection and survivability capabilities however it could have dual use for military and civilian applications. Emphasis of this medical device is on drugs that cannot be practically refrigerated today, allowing for the use of these drugs beyond their current ability.

• Developing a portable biomarker medical device-using novel assays based on QDs and integrated electro-optics system. This technological platform combine the use of functionalized quantum dots and an optical-electronic platform (fiber optics-electro transducer system) for the detection of biomarkers e.g. cortisol, epinephrine. This project has the potential to make a social impact for home detection of cardiac, endocrine and cancer biomarkers.

Post-Doctoral Research Fellow in BioMedical Engineering, with Prof. Jeffrey M. Karp 11/2010-02/2014 Division of Biomedical Engineering, Department of Medicine Center for Regenerative Therapeutics Brigham and Women’s Hospital, Harvard Medical School, Harvard Stem Cell Institute and Harvard-MIT Health Sciences and Technology (HST) Cambridge, Massachusetts, USA.

• Developing and establishing long-acting, sustainable, and efficient micro- and nanoparticle systems to deliver drugs. In this study male SPF Sprague-Dawley rats were used.

• Developing FRET aptamer biosensors that can detect insulin secretion in real-time in vivo from transplanted β-cells (or their precursors). In this study the mouse anterior chamber model is used.

• Engineering cell surfaces to target inflamed pancreas in diabetic mice and monitor their homing to the targeted place as a “Zip Code”. In this study diabetic mouse models are used.

• Engineering Mesenchymal Stem Cells (MSCs) to deliver cargos to inflamed areas. In this study mouse models are used.

• Developing a smart biologically responsive hydrogel platform that releases drugs in response to local expression of enzymes (e.g. MMP-2/MMP-9) that correlates with the level of inflammation in arthritis (Rheumatoid Arthritis). In this study mouse models with induced knee inflammation will be used.

• Immobilizing contract agents on implantable human vein graft for enhancement of vessel wall in MR Imaging.

PhD in Chemistry (Organic/Nanomaterials/Biology), with Prof. Vincent M. Rotello Feb-2011 University of Massachusetts-Amherst, Massachusetts, USA.

• Developed a “chemical nose” technological platform for identifying and detecting cellular components such as proteins, bacteria and cancerous cells as potential medical tool for the early diagnosis of diseases and successful treatment of patients.

• Designed, synthesized, and characterized new small bio-molecules library for biological applications. • Synthesized and characterized nanoparticles library for biological applications. • Engineered nanoparticles surface for biological applications. • Established a functionalized nanoparticle library for biological applications. • Studied nanoparticles’ charge effect on the plasma membrane, up-taking, bio-distribution and pharmacokinetic

profile in vitro and in vivo models.

MSc in Chemistry (Physical Chemistry/Nanomaterials), with Prof. Temer S. Ahmadi Sept-2005 Villanova University, Pennsylvania, USA.

• Studied the effects of intensity and energy of CW UV light on the nucleation and growth of gold nanorods (AuNRs).

• Studied the critical concentrations and role of ascorbic acid (Vitamin C) in the crystallization of gold nanorods within hexadecyltrimethyl ammonium bromide (CTAB)/tetraoctyl ammonium bromide (TOAB) micelles.

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Post-graduate in Business Administration (MBA), with Prof. Ivan Ortiz April-2000 Central American University (UCA), Managua, Nicaragua.

• Determined the competitive and comparative advantages of dairy product clusters in Nicaragua.

BE in Chemical Engineering Sept-1997 With Dr. Ricardo J. Alvarado and Drs. Gabriele and Nikolaus Foidl National University of Engineering (UNI), Managua, Nicaragua.

• Studied the extraction of polyelectrolyte (cationic biopolymer) of Moringa Oleifera seeds and their purification in ultra-filtration of cross-flux and optimization processes using jar test for the treatment of stabilization lakes.

Patents

1. Vincent M. Rotello; Uwe H. F. Bunz; Ronnie L. Phillips; Oscar R. Miranda; Chang-Cheng You. Novel Sensors for Detection of Pathogens, US Application Serial No. 12/313137.

2. Vincent M. Rotello; Uwe H. F. Bunz; Chang-Cheng You; Oscar R. Miranda; Ik-Bum Kim. Detection of Proteins using Nanoparticle-Fluorescent Polymer Sensors, US Application Serial No. 12/313116.

3. Vincent M. Rotello; Uwe H. F. Bunz; Oscar R. Miranda; Avinash Bajaj; Subinoy Rana; Detection of Normal/Cancerous/Metastatic Cells using Nanoparticle-Polymer and Nanoparticle-GFP Supramolecular Complexes. UMA 09-40, Patent Filed, In process.

Awards and Honors

2013 Rheumatology Research Workshop Scholarship Award (ACR and AF), $1,000 June-2013 Brigham and Women’s Hospital-Harvard Medical School

Arthritis Foundation Post-Doctoral Fellowship Award (PF), $100,000 July-2012 Brigham and Women’s Hospital-Harvard Medical School

Translational Medicine Scholarship June-2012 Harvard Catalyst: The Harvard Clinical and Translational Science Center, Harvard Medical School

NIH 2012 Beta Cell Biology Consortium (BCBC) Retreat Scholarship Award, $1,000 May-2012 Harvard-MIT (HST), Harvard Stem Cell Institute (HSCI), Brigham and Women's Hospital and Harvard Medical School

William E. McEwen Outstanding Graduate Student Award, $500 Sept-2009 University of Massachusetts-Amherst

Graduate Student Travel Grant Award, $300 July-2008 University of Massachusetts-Amherst

1st Place SIGMA Xi Research Day May-2005 Villanova University Chapter

Graduate Student Travel Grand Award, $ 500 2x ($1,000) March-2005 Villanova University Professional Experience Research Assistant and Teaching Assistant in Chemistry 10/2005 - 10/2010 University of Massachusetts, Amherst, MA 01003, USA

Chemistry Resource Center Instructor (Integrated Science) Fall 2009 University of Massachusetts, Amherst, MA 01003, USA

Visiting Research Assistant in Chemistry (Prof. Uwe H. F. Bunz) Spring 2008 Georgia Institute of Technology, Atlanta, GA 30332, USA

Research Assistant and Teaching Assistant in Chemistry 09/2002-06/2005 Villanova University, Villanova, PA 19085, USA National Consultant to the United Nations Development 02/15/1997-08/15/2002

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Organization (UNIDO): Project UNIDO US/NIC/02/05, Project UNIDO US/NIC/97/209 and Project UNIDO US/NIC/94/012 Managua, Nicaragua

Researcher, Austrian Project BIOMASS 08/06/1995-02/06/1997 (Austrian Technical Cooperation Organization SUCHER & HOLZER) Managua, Nicaragua

National Technical Specialist in Patents of Inventions 01/15/1993-06/20/1995 Ministry of Economy and Development Managua, Nicaragua

Laboratory Instructor in Chemical Engineering: 03/07/1994-11/25/1995 Organic, Thermodynamic, Mass and Heat Transfer National University of Engineering Managua, Nicaragua Recognition Diplomas of Recognition from 25 enterprises and an International 06/2000 Organization from the World Bank, for helping in the development of microeconomic systems in Nicaraguan rural areas. United Nations Development Organization (UNIDO) Project UNIDO US/NIC/02/05,Project UNIDO US/NIC/97/209 and Project UNIDO US/NIC/94/012 Managua, Nicaragua Professional Affiliation American Diabetes Association, Member 2013-Present

American College of Rheumatology (ACR), Member 2013-Present

The Endocrine Society, Member 2013-Present

American Association for Cancer Research (AACR) 2013-Present

Federation of American Societies for Experimental Biology (FASEB), Member 2013-Present

American Association of Pharmaceutical Scientists (AAPS), Member 2013-Present

Biomedical Engineering Society (BMES), Member 2011-Present

International Society for Stem Cell Research (ISSCR), Member 2011-Present

Controlled Drug Release Society (CRS), Member 2011-Present

Society for Biomaterials, Member 2011-Present

American Institute of Chemical Engineering (AIChE), Member 2011-Present

Materials Research Society (MRS), Member 2005-Present

American Physics Society (APS), Member 2005-Present

Sigma Xi, The Scientific Research, Member 2004-Present

American Chemical Society (ACS) Member 2003-Present

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Publications & Presentations

Refereed Journal Papers (Total papers: 44; Citations: 2675; h-index: 27; i10index: 39) Published (Selective)

1. Pablo Gurman, Oscar R. Miranda, Kevin Clayton, Yitzhak Rosen, Noel Elman. Clinical Applications of Biomedical Micro-devices for Controlled Drug Delivery. Mayo Clinic Proceedings 2015, 90(1):93-108.

2. James A. Ankrum, Oscar R. Miranda, Kelvin S. Ng, Debanjan Sarkar, Chenjie Xu, Jeffrey M. Karp. Engineering cells with intracellular agent-loaded macroparticles to control cell phenotype. Nature Protocols 2014, 9, 233.

3. Subinoy Rana, Rubul Mout, Ngoc D. B. Le, Krishnendu Saha, Gülen Yesilbag Tonga, Oscar R. Miranda, Caren M. Rotello, Vincent M. Rotello. Rapid Cell-Based Mechanistic Profiling of Cancer Therapeutics Using a Multichannel Nanosensor. Nature Nanotech 2014, Accepted (Paper #NNANO-14050870B).

4. Pablo Gurman, Oscar R. Miranda, Alexandra Nathan, Cyrus Washington, Yitzhak Rosen, Noel Elman. Recombinant Tissue Plasminogen Activators (rtPA): A review. Clinical Pharmacology & Therapeutics 2014, Accepted (Paper #2014-0528R).

5. Praveen K. Vemula, Nikken Wiradharma, James A. Ankrum, Oscar R. Miranda, George John, Jeffrey M. Karp. Pro-drugs as self-assembled hydrogels: A new paradigm for biomaterials, Curr. Opin. Biotechnol. 2013, 24, 1174.

6. Krishnendu Saha, Sung Tae Kim, Bo Yan, Oscar R. Miranda, Felix S. Alfonso, Denis Shlosman and Vincent M. Rotello. Surface Functionality of Nanoparticles Determines Cellular Uptake Mechanisms in Mammalian Cells, Small 2013, 9, 300.

Journal Impact Factor (2013)

Nature Nanotechnology 33.265 Nature Chemistry 23.297 Advanced Materials 15.409 Nano Letter 12.940 ACS Nano 12.033 JACS 11.444 Angewandte Chemie International Edition 11.336 PNAS 9.809 Chemical Science 8.601 Current Opinion in Biotechnology 8.035 Nature Protocols 7.782 Current Opinion in Chemical Biology 7.652 Small 7.514 Clinical Pharmacology & Therapeutics 7.390 Chemical Communications 6.718 Nanomedicine 5.824 Mayo Clinic Proceedings 5.812 BioMacromolecules 5.788 Chemistry: A European Journal 5.696 Environmental Science and Technology 5.481 Crystal Growth and Design 4.558 Langmuir 4.384 Soft Matter 4.151 Analytical and Bioanalytical Chemistry 3.578 Plos One 3.534 Journal of Physical Chemistry B 3.377

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7. Subinoy Rana, Arvind K. Singla, Avinash Bajaj, S. Gokhan Elci, Oscar R. Miranda, Rubul Mout, Bo Yan, Frank R. Jirik, and Vincent M. Rotello. Array-Based Sensing of Metastatic Cells and Tissues Using Nanoparticle-Fluorescent Protein Conjugates. ACS Nano 2012, 6, 8233.

8. Rochelle R. Rochelle; Karuna Giri; Daniel Moyano; Oscar R. Miranda; Benjamin Madden; Daniel J. McCormick; Resham Bhattacharya; Vincent M. Rotello; Jean-Pierre Kocher; Priyabrata Murkherjee. Identifying New Therapeutic Targets via Modulation of Protein Corona Formation by Engineered Nanoparticles, PLoS One 2012, 7, e33650.

9. Rochelle R. Arvizo; Oscar R. Miranda; Daniel F. Moyano; Chad A. Walden; Karuna Guri; Resham Bhattacharya; J. David Robertson; Vincent M. Rotello; Joel M. Reid; Priyabrata Mukherjee. Modulating Pharmacokinetics, Tumor Uptake and Biodistribution by Engineered Nanoparticles, PLoS One 2011, 6, e24374.

10. Oscar R. Miranda; Xiaoning Li; Zheng-Jiang Zhu; Bo Yan; Uwe H. F. Bunz; Vincent M. Rotello. Colorimetric bacteria sensing using a supramolecular enzyme-nanoparticle biosensor, J. Am. Chem. Soc. 2011, 133, 9650.

11. Rochelle R. Arvizo; Subinoy Rana; Oscar R. Miranda; Resham Bhattacharya; Vincent M. Rotello; Priyabrata Mukherjee. Mechanism of anti-angiogenic property of gold nanoparticles: Role of nanoparticle size and surface charge, Nanomedicine, 2011, 7, 580. (Front Cover Picture).

12. Oscar R. Miranda; Brian Creran; Vincent M. Rotello. Array-based sensing with nanoparticles: “Chemical noses” for

sensing biomolecules and cell surfaces. Curr. Opin. Chem. Bio. 2010,14, 728.

13. Rochelle R. Arvizo; Oscar R. Miranda; Michael A. Thompson; Christina M. Pabelick; Resham Bhattacharya; J. David Robertson; Vincent M. Rotello; Y. S. Prakash; Priyabrata Mukherjee. Effect of nanoparticle surface charge at the plasma membrane and beyond, Nano Lett. 2010, 10, 2543.

14. Chandramouleeswaran Subramani; Avinash Bajaj; Oscar R. Miranda; Vincent M. Rotello. Biocompatible charged and uncharged surfaces using nanoparticle Films. Adv. Mat. 2010, 22, 5420.

15. Oscar R. Miranda; Hung-Ting Chen; Chang-Cheng You; David E. Mortenson; Xiao-Chao Yang; Uwe H. F. Bunz; Vincent M. Rotello. Enzyme amplified array sensing of proteins in solution and in biofluids, J. Am. Chem. Soc. 2010, 132, 5285.

16. Avinash Bajaj, Oscar R. Miranda; Ronnie L. Phillips, IK-Bum Kim; D. Joseph Jerry, Uwe H. F. Bunz; Vincent Rotello. Array based sensing of normal, cancerous and metastatic cells using conjugated fluorescent polymers, J. Am. Chem. Soc. 2010, 132, 1018.

17. Bo Yan; Zheng-Jiang Zhu; Oscar R. Miranda; Apiwat Chompoosor; Vincent M. Rotello; Richard W. Vachet. Laser desorption/ionization mass spectrometry analysis of monolayer-protected gold nanoparticles, Anal. Bioanal. Chem. 2010, 396, 1025.

18. Avinash Bajaj; Subinoy Rana; Oscar R. Miranda; Joseph C. Yawe; D. Joseph Jerry; Uwe H. F. Bunz; Vincent M. Rotello. Cell surface-based differentiation of cell types and cancer states using a gold nanoparticle-GFP based sensing array, Chem. Sci. 2010, 1, 134.

19. Mrinmoy De; Subinoy Rana; Handan Akpinar; Oscar R. Miranda; Rochelle R. Arvizo; Uwe H. F. Bunz; Vincent M. Rotello. Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescence proteins (GFP), Nature Chem. 2009, 1, 461. (Front Cover Picture)

20. Avinash Bajaj; Oscar R. Miranda; Ik-Bum Kim; Ronnie L. Phillips; D. Joseph Jerry; Uwe H. F. Bunz; ; Vincent M. Rotello. Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays, Proc. Nat. Acad. Sci. (USA) 2009, 106, 10912. (Featured in Chem. & Eng. News (ACS), Science Daily, Times of India, US News and many more) (Among top 20 most read articles in July 2009)

21. Mrinmoy De; Oscar R. Miranda; Subinoy Rana; Vincent M. Rotello. Size dependent protein-nanoparticle self-assembly, Chem. Commun. 2009, 16, 2157.

22. Ronnie Phillips; Oscar R. Miranda; Ik-Bum Kim; David E. Mortenson; Chandramouleeswaran Subramani; Vincent M. Rotello; Uwe H. F. Bunz. Gold nanoparticle-conjugated polymer constructs: Understanding the electrostatic and hydrophobic interactions, Soft Matter 2009, 5, 607.

23. Zheng-Jiang Zhu; Partha S. Ghosh; Oscar R. Miranda; Richard W. Vachet; Vincent M. Rotello. Multiplexed screening of the cellular uptake of gold nanoparticles using Laser Desorption/Ionization Mass Spectrometry (LDI-MS), J. Am. Chem. Soc. 2008, 130, 14139.

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24. Ronnie Phillips; Oscar R. Miranda; Chang-Cheng You; Vincent M. Rotello; Uwe H. F. Bunz. Rapid and efficient identification of bacteria using gold-nanoparticle–poly(para-phenyleneethynylene) constructs, Angew. Chem. Int. Ed. 2008, 47, 2590. (Inside Cover Picture)

25. Oscar R. Miranda; Chang-Cheng You; Ronnie Phillips; Ik-Bum Kim; Partha S. Ghosh; Uwe H. F. Bunz; Vincent M. Rotello. Array-based sensing of proteins using conjugated polymers, J. Am. Chem. Soc. 2007, 129(32); 9856.

26. Chang-Cheng You; Oscar R. Miranda; Basar Gider; Partha S. Ghosh; Ik-Bum Kim; Belma Erdogan; Sai Archana Krovi; Uwe H. F. Bunz; Vincent M. Rotello. Detection and identification of proteins using nanoparticles-fluorescent polymer “chemical nose” sensors, Nature Nanotech. 2007, 2, 318.

27. Oscar R. Miranda; Norman R. Dollahon; Temer S. Ahmadi. Critical concentrations and role of ascorbic acid (Vitamin C) in the crystallization of gold nanorods within hexadecyltrimethyl ammonium bromide (CTAB)/tetraoctyl ammonium bromide (TOAB) micelles, Cryst. Growth Des. 2006, 6, 2747.

28. Oscar R. Miranda; Temer S. Ahmadi. Effects of intensity and energy of CW UV-Light on the growth of gold nanorods, J. Phys. Chem. B 2005, 109, 15724

Additional published

29. Yi-Cheun Yeh; Krishnendu Saha; Bo Yan; Xi Yu; Oscar R. Miranda; Vincent M. Rotello. The Role of Ligand Coordination on the Cytotoxicity of Cationic Quantum Dots. Nanoscale 2013, 5, 12140.

30. Zheng-Jiang Zhu; Rui Tang; Yi-Cheun Yeh; Oscar R. Miranda; Vincent M. Rotello; Richard W. Vachet. Determination of the Intracellular Stability of Gold Nanoparticle Monolayers Using Mass Spectrometry, Anal. Chem. 2012, 84, 4321.

31. Steven C. Hayden; Gengxiang Zhao; Krishnendu Saha; Ronnie L. Phillips; Xiaoning Li; Oscar R. Miranda; Vincent M. Rotello; Mostafa A. El-Sayed; Ingeborg Schmidt-Krey; Uwe H. F. Bunz. Aggregation and Interaction of Cationic Nanoparticles on Bacterial Surfaces, J. Am. Chem. Soc. 2012, 134, 6920.

32. Daniel F. Moyano; Meir Goldsmith; David J. Solfiell; Dalit Landesman-Milo; Oscar R. Miranda; Dan Peer; Rotello, Vincent M. Rotello. Nanoparticle Hydrophobicity Dictates Immune Response, J. Am. Chem. Soc. 2012, 134, 3965.

33. Xi Yu; Jonathan T. Pham; Chandramouleeswaran Subramani; Brian Creran; Yi-Cheun Yeh; Du; Debabatra Patra, Oscar R. Miranda, Crosby AJ, Vincent M. Rotello. Direct Patterning of Engineered Ionic Gold Nanoparticles via Nanoimprint Lithography. Adv Mater. 2012, 24, 6330.

34. Subinoy Rana; Xi Yu; Debabrata Patra; Daniel F. Moyano; Oscar R. Miranda; Irshad Hussain; Vincent M. Rotello. Control of Surface Tension at Liquid-Liquid Interfaces Using Nanoparticles and Nanoparticle-Protein Complexes, Langmuir 2012, 28, 2023.

35. Kaimin Chen; Yisheng Xu; Subinoy Rana; Oscar R. Miranda; Paul L. Dubin; Vincent M. Rotello; Lianhong Sun; Xuhong Guo. Electrostatic selectivity in protein−nanoparticle interactions, Biomacromolecula 2011, 12, 2552.

36. Yi-Cheun Yeh; Debabrata Patra; Bo Yan; Oscar R. Miranda; Vincent M. Rotello. Synthesis of cationic quantum dots via one/two step ligand exchange reaction. Chem. Comm. 2010, 47, 3069.

37. Psaras L. McGrier; Kyril M. Solntsev; Anthony J. Zucchero; Oscar R. Miranda; Vincent M. Rotello; Laren M. Tolbert; Uwe H. F. Bunz. Hydroxydialkylamino Cruciforms: Amphoteric Materials with Unique Photophysical Properties, Chem. Eur. J. 2011, 17, 3112.

38. Xiao-Chao Yang; Bappaditya Samantha; Sarit S. Agasti; Youngdo Jeong; Zheng-Jiang Zhu; Subinoy Rana; Oscar R. Miranda; Zhi-Hong Mo; Vincent M. Rotello. Particle stabilized nanocapsule as a lipophilic drug carrier, Angew. Chem. Int. Ed. 2011, 50, 477.

39. Apiwat Chompoosor; Krishnendu Saha; Partha S. Ghosh; Dylan J. Macarthy; Oscar R. Miranda; Zhenjiang Zhu; Kathleen F. Arcaro; Vincent M. Rotello. DNA damage caused by cationic gold nanoparticles in mammalian cell: The potential use of gold nanoparticles as therapeutics, Small 2010, 6, 2246.

40. Zheng-Jiang Zhu; Rachel Carboni; Michael Quercio; Bo Yan; Oscar R. Miranda; Douglas L Ardenton; Kathleen F. Arcaro; Vincent M. Rotello; Richard W. Vachet. Effect of surface chemistry on uptake, distribution, and excretion of nanomaterials by Japanese medaka. Small 2010, 6, 2261.

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41. Debabrata Patra; Firat Ozdemir; Oscar R. Miranda; Bappaditya Samanta; Amitav Sanyal; Vincent M. Rotello. Formation and size tuning of colloidal microcapsules via host−guest molecular recognition at the liquid−liquid interface, Langmuir 2009, 25, 13852.

42. Bappaditya Samanta; Xiao-Chao Yang; Yuval Ofir; Myoung-Hawn Park; Debabrata Patra; Sarit S. Agasti; Oscar R. Miranda; Zhi-Hong Mo; Vincent M. Rotello. Catalytic microcapsules assembled from enzyme-nanoparticle conjugates at oil-water interfaces, Angew. Chem. Int. Ed. 2009, 48, 5341.

43. Myoung-Hwan Park; Yuval Ofir; Bappaditya Samanta; Palaniappan Arumugam; Oscar R. Miranda; Vincent M. Rotello. Nanoparticle immobilization on surfaces via activatable heterobifunctional dithiocarbamate bond formation, Adv. Mat. 2008, 20, 4185.

44. Psaras L. McGrier; Kyril M. Solntsev; Shaobin Miao; Laren M. Tolbert; Oscar R. Miranda; Vincent M. Rotello; Uwe H. F. Bunz. Hydroxycruciforms: amine-responsive fluorophores, Chem. Eur. J. 2008, 14, 4503. (Front Cover Picture).

Submitted and in Preparation

1. Oscar R. Miranda, Hilla Conen, Noel M. Elman. Rapid Reconstitution Packages (RRPs) for Emergency Delivery of Glucagon. 2015, Submitted.

2. David Veysett, Oscar R. Miranda, Pablo Gurman, Roni Cantor, Keith Nelson, Noel M. Elman. Laser Activated Nanoparticle Delivery for Tumor Therapy. 2015, In preparation.

3. Oscar R. Miranda, Keith Whitaker, Kaleb G. MacDowell, Moungi Bawendi, Noel M. Elman. Quantum-Dot based Assay for Rapid and Specific Ex Vivo Detection of Salivary Cortisol. 2014, In Preparation.

4. Oscar R. Miranda†, Hilde De-Man†, Bryan E. Laulicht, Patrick Sterkens, Praveen K. Vemula, Weian Zhao, James A. Ankrum, Kelvin S. Ng, Nikken Wiradharma, Shrey Sindhwani, Liang Zhang, Lijie Zhang, Chenjie Xu, Katie Amssoms, Jeffrey M. Karp*. Long-acting injectable drug delivery matrix system: A TCM15 pharmacokinetic “in vivo” study for HIV treatment, 2015, In preparation.

5. Dimitrios Mitsouras, Ming Tao, Oscar R. Miranda, Frank J. Rybicki III, Charles K. Ozaki, Jeffrey M. Karp, Peng Yu, Praveen Vemula, Chenwei Liu, Robert Mulkern. Vein grafts implantable contrast MRI Initiative: Enhanced Vessel Wall Imaging, 2015, In Preparation.

6. Oscar R. Miranda†, Oren Levy†, Yen Sun, Luke Mortensen, Kelvin Ng, Shrey Sindhwani, Nikken Wiradharma, Jason Gaglia, Stephan Kissler, Charles Lin and Jeffrey M. Karp*. Engineering MIN6 cells surface to target inflamed pancreas in diabetic mice models: A novel zip code approach for pancreatic cells transplantation, 2015, In process.

7. Oscar R. Miranda; Shrey Sindhwani; Sandy Spurr-Michaud; Bryan Laulicht, Eoin O’Cearbhaill, James A. Ankrum, Grace Sock Leng Teo, Ilene K. Gipson; Jeffrey M. Karp*. Surface engineering and size effect of mucoadhesive particles for ophthalmic drug delivery in an “in vitro” ocular cells model, 2015, In process.

8. Oscar R. Miranda†; Praveen K. Vemula†; Anita Tseng Shaw; Ellen M. Gravallese; Antonios Aliprantis; Jeffrey M. Karp*. Inflammation responsive hydrogels for treatment of inflammatory arthritis, 2015, In process.

Conference presentations

1. Oscar R. Miranda, Frank J. Rybicki III, Charles K. Ozaki, Jeffrey M. Karp, Peng Yu, Dimitrios Mitsouras, Praveen Vemula, Ming Tao, Chenwei Liu, Robert Mulkern. Vein grafts implantable contrast MRI Initiative: Enhanced Vessel Wall Imaging. 99th Scientific Assembly and Annual Meeting of the Radiological Society of North America (RSNA), Chicago, IL, United Stated, December 1-6, 2013.

2. Oscar R. Miranda, Praveen K. Vemula, Oren Levy, Michael Valic, Priya Anand, Ellen M. Gravallese, Antonios O. Aliprantis, Jeffrey M. Karp. Biologically responsive hydrogels: A new approach for treatment of inflammatory arthritis, 2013 Rheumatology Research Workshop (American College of Rheumatology (ACR) and Arthritis Foundation (AF)), Dallas, TX, United States, June 21-23, 2013.

3. Oscar R. Miranda, Praveen K. Vemula, Oren Levy, Michael Valic, Priya Anand, Ellen M. Gravallese, Antonios O. Aliprantis, Jeffrey M. Karp. Biologically responsive hydrogels: A new approach for treatment of inflammatory arthritis, BWH BRI Regenerative Medicine Center Symposium, Boston, MA, United States, June 11, 2013.

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4. Oscar R. Miranda, Yen Sun, Shrey Sindhwani, Kelvin Ng, Maike Sander, Charles Lin, Jeffrey M. Karp. Development of an approach to monitor real-time insulin secretion in vivo at the single cell level, NIH 2012 Beta Cell Biology Consortium (BCBC) Retreat, Chantilly, VA, United States, May 1-4, 2012.

5. Oscar R. Miranda; Avinash Bajaj; Sukru G. Elci; Subinoy Rana; Krishnendu Saha; Ronnie L. Phillips; Jonathan J. Bryant; Ik-Bum Kim; D. Joseph Jerry; Uwe H. Bunz; Vincent M. Rotello. FRET based pattern recognition of isogenic cells differing in cell surface glycans. ORGN-694, CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

6. Oscar R. Miranda; Xiaoning Li; Zheng-Jiang Zhu; Bo Yan; Uwe H. F. Bunz; Vincent M. Rotello. Colorimetric bacteria sensing using a hybrid enzymatic nanocomposite biosensor. COLL-46, CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

7. Oscar R. Miranda; Hung-Ting Chen; Chang-Cheng You; David E. Mortenson; Vincent M. Rotello. A novel gold nanoparticle-based protein sensor with enzyme-substrate reactions as signal amplifiers. COLL-131, CODEN: 69KXQ2, 236th ACS National Meeting, Philadelphia, PA, United States, August 17-21, 2008.

8. Oscar R. Miranda; Hung-Ting Chen; Chang-Cheng You; Ronnie L. Phillips; Ik-Bum Kim; Uwe H. F. Bunz; Vincent M. Rotello. “Chemical nose” sensing and identification of proteins and bacteria. III International Symposium on Microcyclic and Supramolecular Chemistry (ISMSC), Las Vegas, NV, United States, July 13-18, 2008.

9. Oscar R. Miranda; Chang-Cheng You; Ronnie L. Phillips; Ik-Bum Kim;Partha S. Ghosh; Uwe H. F. Bunz; Vincent M. Rotello. Ultraversatile protein sensor based on an array of conjugated polymers. ORGN-227, CODEN: 69JNR2; 234th ACS National Meeting, Boston, MA, United States, August 19-23, 2007.

10. Oscar R. Miranda; Temer S. Ahmadi. Substrate-Assisted Phase Transitions of Au Nanorods. GENE-520, CODEN: 69GVWG; 37th Middle Atlantic Regional Meeting of the American Chemical Society, New Brunswick, NJ, United States, May 2005.

11. Oscar R. Miranda; Riffard Jean-Gilles; Temer Ahmadi. Nucleation and Growth of Gold Nanorods. Villanova University, Sigma Xi Research Day, Villanova, PA, United States, April 2005.

12. Oscar R. Miranda; Riffard Jean-Gilles; Temer S. Ahmadi. Growth Kinetics of Gold Nanorods. The16th Annual Saint Josepth’s University SIGMA XI, Student Research Symposium, Philadelphia, PA, United States, April 2005.

13. Oscar R. Miranda; Riffard Jean-Gilles; Temer S. Ahmadi. Effects of Chemical and Physical Parameters on the Growth Kinetics of Gold Nanorods. PHYS-384, CODEN: 69GQMP; 229th ACS National Meeting, San Diego, CA, United States, March 2005 (Science Mix).

14. Oscar R. Miranda; Temer S. Ahmadi. Mechanism and Growth Kinetics of Colloidal Gold Nanoparticles, COLL-076, CODEN: 69FTZ8; 228th ACS National Meeting, Philadelphia, PA, United States, August 2004.

15. Oscar R. Miranda; Temer S. Ahamadi. 2- and 3-Dimentional Assembly of Shape-selected and Surface-modified Au Nanoparticles: Nature of Packing and its Correlation to Optical Properties; PHYS-360, CODEN 69EKY9; 226th ACS National Meeting, New York City, NY, United States, September 2003.

Other Conference Presentations

16. Rochelle R. Arvizo; Subinoy Rana; Oscar R. Miranda; Resham Bhattacharya; Vincent M. Rotello; Priyabrata Mukherjee. Mechanism of anti-angiogenic property of gold nanoparticles: Role of nanoparticle size and surface charge. COLL-295. CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

17. Xi Yu; Kan Du; Craig Versek; Debabrata Patra; Oscar R. Miranda; Anthony D. Dinsmore; Mark T. Tuominen; Vincent M. Rotello. Soft and deformable gold nanoparticles with unique conducting properties. ORGN-832. CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

18. Subinoy Rana; Avinash Bajaj; Oscar R. Miranda; Joseph C. Yawe; D. J. Jerry; Uwe H. Bunz; Vincent M. Rotello. Cell-surface and lysate based identification of cell types and cancer states using gold nanoparticle-GFP supramolecular complexes. ORGN-260, CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

19. Zheng-Jiang Zhu; Bo Yan; Oscar R. Miranda; Richard W. Vachet; Vincent M. Rotello Characterization and tracking of monolayer-protected nanoparticles in biological systems using mass spectrometry. COLL-234, CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

10

20. Subinoy Rana; Mrinmoy De; Oscar R. Miranda; Uwe H. Bunz; Vincent M. Rotello. Array based sensing of proteins in human serum using nanoparticle-green fluorescent protein conjugates. COLL-48, CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

21. Zheng-Jiang Zhu; Rachel Carboni; Michael Quercio; Bo Yan; Oscar R. Miranda; Kathleen F. Arcaro; Richard W. Vachet; Vincent M. Rotello. Effect of surface chemistry on uptake, distribution and excretion of nanomaterials by Japanese medaka. INOR-721, CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

22. Rochelle R. Arvizo; Priyabrata Mukherjee; Y. S. Prakash; Michael A. Thompson; Oscar R. Miranda; J. D. Robertson; Vincent M. Rotello. Surface charge determines functional interactions between nanoparticles and cells. COLL-303. CODEN: 69NAQG, 240th ACS National Meeting, Boston, MA, United States, August 22-26, 2010.

23. Zheng-Jiang Zhu; Partha Ghosh; Oscar R. Miranda; Richard W. Vachet; Vincent M. Rotello. Multiplexed screening of the cellular uptake of gold nanoparticles using Laser Desorption/Ionization Mass Spectrometry. ANYL-204, CODEN: 69KXQ2, 236th ACS National Meeting, Philadelphia, PA, United States, August 17-21, 2008.

24. Chang-Cheng You; Oscar R. Miranda; Basar Gider; Partha S. Ghosh; Ik-Bum Kim; Uwe H. F. Bunz; Vincnet M. Rotello. Detection and identification of proteins based on nanoparticle-fluorescent polymer arrays. ORGN-756, CODEN: 69JAUY; 233rd ACS National Meeting, Chicago, IL, United States, March 25-29, 2007.

25. Megan Daley; Oscar R. Miranda; Norman R. Dollahon; Temer S. Ahmadi. Observation of a Critical Concentration of L-Ascorbic Acid (Vitamin C) in the Photochemical Synthesis of Au Nanorods. MRM-445, CODEN: 69ICW3; 38th Middle Atlantic Regional Meeting of the American Chemical Society, Hershey, PA, United States, June 4-7, 2006.

26. Charles C. Farraioli; Oscar R. Miranda; Norman R. Dollahon; Temer S. Ahmadi. Interplay of Temperature and UV-Light in the Growth Process of Au-Nanorods. MRM-444, CODEN: 69ICW3; 38th Middle Atlantic Regional Meeting of the American Chemical Society, Hershey, PA, United States, June 4-7, 2006.

References

• Dr. Noel M. Elman, Principal Research Scientist, Massachusetts Institute of Technology Biomedical Devices and Translational Research Lab, Institute for Soldier Nanotechnologies (ISN) 500 Technology Square, NE47-525, Cambridge, MA 02139 Phone: (617) 324-6447, e-mail: nelman@mit.edu

• Prof. Jeffrey M. Karp, Associate Professor, Harvard Medical School Harvard-MIT Health Sciences and Technology Division (HST) Division of Biomedical Engineering, Department of Medicine Brigham and Women's Hospital, Harvard Medical School 65 Landsdowne Street, PRB Room 313, Cambridge, MA 02139

Phone: (617) 817-9174, e-mail: jeffkarp.bwh@gmail.com

• Prof. Vincent M. Rotello, Charles A. Goessmann Professor, Department of Chemistry University of Massachusetts-Amherst 379A LGRT Tower A, 710 North Pleasant Street, Amherst, MA 01003. Phone: (413)-545-2058, e-mail: rotello@chem.umass.edu

• Prof. Temer S. Ahmadi, Associate Professor of Chemistry Director, Chemistry Graduate Program Villanova University Mendel Hall Rm 214F, Chemistry Department 800 E. Lancaster Avenue, Villanova, PA 19085

Phone: (610) 519-7796, temershah.ahmadi@villanova.edu

• Prof. Uwe H. F. Bunz, Professor (W3) Institut für Organische Chemie, Ruprecht-Karls-Universität Heidelberg Im Neuenheimer Feld 270, Heidelberg 69120, Germany

Phone : +49 (622) 154-8401, e-mail : Uwe.Bunz@oci.uni-heidelberg.de