orienting human stem cells ( hmscs ) by means of electrospun polymer nanofibers
DESCRIPTION
Orienting Human Stem Cells ( hMSCs ) by Means of Electrospun Polymer Nanofibers. Investigators: M. Cho, Bioengineering; A. Yarin , C. M. Megaridis , Mechanical and Industrial Engineering; E . Zussman , Technion -Israel. Oriented. Random. - PowerPoint PPT PresentationTRANSCRIPT
Problem Statement and Motivation
Key Achievement and Future GoalsTechnical Approach
Orienting Human Stem Cells (hMSCs) by Means of Electrospun Polymer Nanofibers
Investigators: M. Cho, Bioengineering; A. Yarin, C. M. Megaridis, Mechanical and Industrial Engineering; E. Zussman, Technion-Israel
• Cell orientation and adhesion control the functionality of natural and engineered tissues
• Electrospinning is a low-cost technique which can produce polymer nanofibers aligned along a specific direction
• Polymer nanofibers can be used to mimic the native extracellular matrix (ECM) features
• Electrospun polymer nanofiber scaffolds are used to manipulate cell orientation and adhesion
• hMSCs adhered and oriented along PCL nanofibers
• During long-term culture, hMSCs demonstrated no preferred orientation on random nanofibrous scaffolds; cells consistently aligned on oriented scaffolds
• Oriented PCL nanofibrous scaffolds could be used to mimic the cell and ECM organization in the native tissue, such as muscle, tendon, and the superficial zone of articular cartilage
• The fiber scaffold/hSMC approach holds promise for a variety of tissue engineering applications
• Random and oriented polycaprolactone (PCL) nanofibrous scaffolds produced using electrospinning
• hMSCs were cultured and seeded on two scaffold types (random, oriented)
• Orientations of hMSCs and nanofibers on random and oriented nanofibrous scaffold samples were measured via laser scanning confocal microscopy at different time points during an 18-day culture period
• hMSC viability tests were performed to verify compatibility of the cells with the PCL
A2
A3
B2
B3
B1A1
A2
A3
B2
B3
B1A1
Random Oriented
Cells: Green, Nanofibers: Red