organ printing - texas tech university health sciences center · organ printing: a computer-aided,...
TRANSCRIPT
Organ printing:A computer-aided, layer-by-layer additive biofabrication of 3D
functional human tissue and organ constructs using self-assembling
tissue spheroids as building blocks.
Tissue fusion Tissue maturationBiofabrication of a vascular tree
Microsphere
as bioink
Biopaper
3D tissue fabrication process
3D bioprinting
techniques
Orifice-based
printing
Orifice-free
printing
Inkjet-based printing
Filament-based printing
Laser-based printing
3D cellular
tube being
printed
Z-shape platform
attached to Z
stage
Container
with
CaCl2
α
LED strobe Camera
Moving
direction
Dispense head
attached to XY stages
Bioink (3T3
cell
suspension
and NaAlg)
D
H
t
OXY
Z
CaCl2 solution level
Cells
Fig. (a) Printed tube and (b) tube surface and (c) cell viability test after liquefying
(a)
Cell
(b)
Live Cell
Dead Cell
After liquefying
(c)
[Murphy2014]
[Hockaday2012]
[Murphy2014] [Brown2010]
[Koch2012]
[Miller2012]
3D lattice of filaments
References