Unique Ecological Hosts for Choclo Virus in Panama and Maporal Virus in

Venezuela: Evidence They Are Distinct Species

Unique Ecological Hosts for Choclo Virus in Panama and Maporal Virus in

Venezuela: Evidence They Are Distinct Species

orWhen a mouse isn’t just a mouse.

J. Delton Hanson*, Charles F. Fulhorst, Antonio Utera

orWhen a mouse isn’t just a mouse.

J. Delton Hanson*, Charles F. Fulhorst, Antonio Utera

HantavirusesHantaviruses

• RNA viruses– Small– Medium– Large (think Goldilocks)

• Old World – Hemorrhagic fever w/Renal Syndrome (1950’s)

• RNA viruses– Small– Medium– Large (think Goldilocks)

• Old World – Hemorrhagic fever w/Renal Syndrome (1950’s)

HantavirusesHantaviruses

• New World – Hantavirus Cardiopulmonary Syndrome (HPS)– Sin Nombre (1993)– Others identified– Not all cause HPS– Not all HPS’s fatal

• New World – Hantavirus Cardiopulmonary Syndrome (HPS)– Sin Nombre (1993)– Others identified– Not all cause HPS– Not all HPS’s fatal

HantavirusesHantaviruses

• Host - Virus

• 1:1 Long-term relationships– Requirement for species recognition

• Host - Virus

• 1:1 Long-term relationships– Requirement for species recognition

HantavirusesHantaviruses

• Large distribution follows host• Large distribution follows host

Peromyscus leucopusPeromyscus maniculatus

HantavirusesHantaviruses

• Large distribution follows host• Large distribution follows host

HantavirusesHantaviruses

• Large distribution follows host• Large distribution follows host

HantavirusesHantaviruses• Large distribution follows host

Rio mamore virusOligoryzomys microtis

• Large distribution follows host

Rio mamore virusOligoryzomys microtis

*

*

*

**

0 500Kilometers

Richter, Hanson, Cajimat, Milazzo, and Fulhorst. Geographical range of Rio Mamoré virus (family Bunyaviridae, genus Hantavirus) in association with the small-eared pygmy rice rat (Oligoryzomys microtis). Vector-Borne and Zoonotic Diseases.

HantavirusesHantaviruses

• Closely related viruses w/closely related hosts– Oryzomys sp w/ Bayou and Catacamas

viruses

• Closely related viruses w/closely related hosts– Oryzomys sp w/ Bayou and Catacamas

viruses

HantavirusesHantaviruses

• Closely related viruses w/closely related hosts– Sigmodon sp w/ Muleshoe and Blackcreek

Canal

• Closely related viruses w/closely related hosts– Sigmodon sp w/ Muleshoe and Blackcreek

Canal

HantavirusesHantaviruses

Bradley et al. 2008

HantavirusesHantaviruses

Bradley et al. 2008

HantavirusesHantaviruses

• 2000 – Choclo virus – HPS– Panama– Oligoryzomys fulvescens (fulvous pygmy rice

rat)

• 2004 – Maporal virus – no HPS?– Venezuela– Oligoryzomys fulvescens (fulvous pygmy rice

rat)

• Violates 1:1 assumption

• 2000 – Choclo virus – HPS– Panama– Oligoryzomys fulvescens (fulvous pygmy rice

rat)

• 2004 – Maporal virus – no HPS?– Venezuela– Oligoryzomys fulvescens (fulvous pygmy rice

rat)

• Violates 1:1 assumption

OligoryzomysOligoryzomys

• Small, long tailed

• Grass adapted (Reithrodontomys)

• Peri-domestic

• Small, long tailed

• Grass adapted (Reithrodontomys)

• Peri-domestic

Oligoryzomys fulvescens

Oligoryzomys fulvescens

Rogers, D. S., D. K. Hardy, J. D. Hanson, E. A. Arellano, N. Lewis-Rogers and F. X. González-Cózatl. 2009. Molecular phylogenetics of Oligoryzomys fulvescens based on cytochrome b gene sequences, with comments on the evolution of the genus Oligoryzomys. Miranda, GB, Oliveira, LFB, Andrades-Miranda, J, Langguth, A, et al. Phylogenetic and phylogeographic patterns in sigmodontine rodents of the genus Oligoryzomys.

?

TPMV-VRC 66412-Ind

THAIV-Thai749-Tha

SEOV-80·39-SKor

HTNV-76·118-SKor

DOBV-Ano·Poroia-Gre

KHAV-MF·43-Rus

TOPV-Ls136v-Rus

PUUV-Sotkamo-Fin

PHV-PH·1-USA

TULV-Moravia/5302v/95-CzeR

CADV-VHV·574-Ven

ANDV-Chile·9717869-Chi

LANV-510B-Par

RIOMV-HTN·007-Per

MAPV-HV·97021050-Ven

CHOV-588-Pan

BAYV-Hu_La-USA

CATV-HV·C1280001-Hon

BCCV-USA

NYV-RI·1-USA

SNV-Convict Creek 107-USA

ELMCV-RM·97-USA

0.3

O. vegetus

O. messorius

O. delicatus

O. moojeni

O. destructorO. longicaudatusO. andinus

O. nigripesO. stramineus

O. costaricensis

O. fulvescens

O. fornesiO. sp1

O. microtis

O. chacoensis

0.1

Outgroup

O. flavescens

O. vegetus

O. messorius

O. delicatus

O. moojeni

O. destructorO. longicaudatusO. andinus

O. nigripesO. stramineus

O. costaricensis

O. fulvescens

O. fornesiO. sp1

O. microtis

O. chacoensis

0.1

Outgroup

O. flavescens

11.06%

8.99%9.73%

O. vegetus

O. messorius

O. delicatus

O. moojeni

O. destructorO. longicaudatusO. andinus

O. nigripesO. stramineus

O. costaricensis

O. fulvescens

O. fornesiO. sp1

O. microtis

O. chacoensis

0.1

Outgroup

O. flavescens

1.74%

1.99%

1.25%

TPMV-VRC 66412-Ind

THAIV-Thai749-Tha

SEOV-80·39-SKor

HTNV-76·118-SKor

DOBV-Ano·Poroia-Gre

KHAV-MF·43-Rus

TOPV-Ls136v-Rus

PUUV-Sotkamo-Fin

PHV-PH·1-USA

TULV-Moravia/5302v/95-CzeR

CADV-VHV·574-Ven

ANDV-Chile·9717869-Chi

LANV-510B-Par

RIOMV-HTN·007-Per

MAPV-HV·97021050-Ven

CHOV-588-Pan

BAYV-Hu_La-USA

CATV-HV·C1280001-Hon

BCCV-USA

NYV-RI·1-USA

SNV-Convict Creek 107-USA

ELMCV-RM·97-USA

0.3

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

• A mouse is not a mouse• A mouse is not a mouse

O. species 1

O. species 2

couesi

palustris

mexicanus

O. species 1

O. species 2

couesi

texensispalustris

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

• A virus is not a virus– HPS vs non HPS– Fatal vs un-noticeable

• A virus is not a virus– HPS vs non HPS– Fatal vs un-noticeable

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

• You know what they say about assuming…

• You know what they say about assuming…

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

Photo by Michael Patrikeev

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

Photo by Michael Patrikeev

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

Photo by Michael Patrikeev

Take Home Conclusionsi.e. why should you care about mice in Central

America

Take Home Conclusionsi.e. why should you care about mice in Central

America

• Deposit vouchers!!!• Deposit vouchers!!!

AcknowledgmentsAcknowledgments• Abilene Christian University Natural

History Collections – Tom Lee• American Museum of Natural History –

Rob Voss, Marcelo Weksler• Angelo State University – Loren

Ammerman, Robert Dowler• Carnegie Museum of Natural History –

Sue McLaren• Monte L. Bean Life Science Museum –

Duke Rogers• Museu Nacional Rio de Janerio –

Cibele Bonvincino, Jaoa Oliveria• Field Museum of Natural History –

Bruce Patterson• Instituto de Cienceias Naturales• University of Kansas Natural History

Museum – Bob Timm

• Abilene Christian University Natural History Collections – Tom Lee

• American Museum of Natural History – Rob Voss, Marcelo Weksler

• Angelo State University – Loren Ammerman, Robert Dowler

• Carnegie Museum of Natural History – Sue McLaren

• Monte L. Bean Life Science Museum – Duke Rogers

• Museu Nacional Rio de Janerio – Cibele Bonvincino, Jaoa Oliveria

• Field Museum of Natural History – Bruce Patterson

• Instituto de Cienceias Naturales• University of Kansas Natural History

Museum – Bob Timm

• Museo de Historia Natural La Salle• Museo Nacional de Historia Natural –

Guillermo D’Elia• Museum of Southwestern Biology –

Jon Dunnum, Cheryl Parameter• Museum of Vertebrate Zoology –

James Patton, Chris Conroy• Royal Ontario Museum – Mark

Engstrom, Burton Lim• Texas Tech University – Robert J.

Baker, Heath Garner, Kathy MacDonald

• National Museum of Natural History – Jeremy Jacobs, Michael Carleton

• Robert Owen, Jane Indorf

• Museo de Historia Natural La Salle• Museo Nacional de Historia Natural –

Guillermo D’Elia• Museum of Southwestern Biology –

Jon Dunnum, Cheryl Parameter• Museum of Vertebrate Zoology –

James Patton, Chris Conroy• Royal Ontario Museum – Mark

Engstrom, Burton Lim• Texas Tech University – Robert J.

Baker, Heath Garner, Kathy MacDonald

• National Museum of Natural History – Jeremy Jacobs, Michael Carleton

• Robert Owen, Jane Indorf