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OOSIGHT IMAGING SYSTEMS

Adding Oosight to your lab can improve success by

offering you a quantitative and reproducible method

to measure biological disruption in either fresh or

previously frozen oocytes. You can now select against

oocytes for ICSI and embryos for implantation, and use

the system to dramatically improve enucleation efficiency.

Understanding the oocyte is critical to understanding

the foundation of embryogenesis. Studies show that a

disrupted spindle apparatus or a weakened zona pellucida

in the oocyte have yielded lower pregnancy rates. In fact,

pregnancy is 8-times more likely when the inner zona

pellucida is well-ordered. 1

Oosight. The non-invasive imaging system that reveals critical structures

in the oocyte to improve egg grading and enable new discoveries.

1Shen Y, et al. High magnitude of light retardation by the zona pellucida is associated with conception cycles. Human Reproduction, 2005 Jun;20(6):1596-606. Photography credits • Front cover – Retardance pseudocolor image of MII human oocyte • Back cover – Retardance grayscale image of MII human oocyte • Before/After image above spec chart – courtesy of RMA NJ

Immature Oocyte with Extruded PB

Oosight outperforms other systems.

No other contrast-enhancement technique delivers the performance of Oosight. With its unprecedented resolution and calibrated setup, Oosight provides the reproducibility and sensitivity required of a grading routine, as well as the speed needed for micromanipulation.

Improve the efficiency of oocyte cryopreservation.

Whenever a clinical application is

known to negatively alter the state

of the biological material being

used, it is imperative that checks and

balances are in place to monitor the

extent of that change. Oosight can

help do this for all cryopreserved

oocytes by providing a method that

helps ensure that vital structures

in the oocyte are reformed to their

original, pre-frozen state.

Oosight image of human MII oocyte taken just prior to ICSI. The spindle apparatus is nicely barrel shaped and the three layers of the zona pellucida are all visible.

after

Oocyte screening can make all the difference.

Oosight enables you to determine

which subpopulation of oocytes

are at high risk for producing

chromosomally abnormal

embryos. One in approximately

20 cycles contains oocytes that

are immature but are nevertheless

falsely labeled MII using

conventional imaging techniques.

Oosight can prevent the

potentially damaging effects that

result from injecting immature

oocytes. The system can also help

screen for oocytes with highly

disrupted spindles, such as those

that are multipolar.

It’s an easy add-on.

The Oosight system integrates

seamlessly onto your ICSI

workstation. Now with foot-pedal

controls and automated spindle

and zona tools, measuring each

oocyte is a simple 2-step process.

Snap an image and then press

a button to report the data.

Meaningful retardance and

orientation data are organized into

an intuitive, exportable report. It’s

really that simple.

Contact us at 1-800-383-7924 or at www.cri-inc.com/oosight to learn more about Oosight or to request a quotation of this breakthrough system.

microscope not included

From the conventional constrast “before” image, structures such as the spindle and multiple layers of the zona pellucida remain invisible.

Orientation Color Map

Orientation Vector Map

Retardance Color Map

Automated Spindle FinderTM

Automated Inner-zona FinderTM

Images to the right display the various analysis tools in the Oosight software.

before

Oosight outperforms other systems.

No other contrast-enhancement technique delivers the performance of Oosight. With its unprecedented resolution and calibrated setup, Oosight provides the reproducibility and sensitivity required of a grading routine, as well as the speed needed for micromanipulation.

Improve the efficiency of oocyte cryopreservation.

Whenever a clinical application is

known to negatively alter the state

of the biological material being

used, it is imperative that checks and

balances are in place to monitor the

extent of that change. Oosight can

help do this for all cryopreserved

oocytes by providing a method that

helps ensure that vital structures

in the oocyte are reformed to their

original, pre-frozen state.

Oosight image of human MII oocyte taken just prior to ICSI. The spindle apparatus is nicely barrel shaped and the three layers of the zona pellucida are all visible.

after

Oocyte screening can make all the difference.

Oosight enables you to determine

which subpopulation of oocytes

are at high risk for producing

chromosomally abnormal

embryos. One in approximately

20 cycles contains oocytes that

are immature but are nevertheless

falsely labeled MII using

conventional imaging techniques.

Oosight can prevent the

potentially damaging effects that

result from injecting immature

oocytes. The system can also help

screen for oocytes with highly

disrupted spindles, such as those

that are multipolar.

It’s an easy add-on.

The Oosight system integrates

seamlessly onto your ICSI

workstation. Now with foot-pedal

controls and automated spindle

and zona tools, measuring each

oocyte is a simple 2-step process.

Snap an image and then press

a button to report the data.

Meaningful retardance and

orientation data are organized into

an intuitive, exportable report. It’s

really that simple.

Contact us at 1-800-383-7924 or at www.cri-inc.com/oosight to learn more about Oosight or to request a quotation of this breakthrough system.

microscope not included

“Because spindle screening can improve efficiency of gamete selection, it should be considered when choosing eggs for freezing or fertilization by ICSI.” Dr. N Noyes, NYU IVF Program

On the left, human MII oocyte has a normal barrel-shaped spindle (azimuth skew from barrel shape is 5.12) . On the right, this human MII oocyte has an abnormal tri-polar spindle (azimuth skew from barrel shape is 49.4). Inner zona and spindle retardances are not shown, but these data are provided by the Oosight system as well.

normal abnormal

*The Spindle And Zona Finder are tools in the Oosight software used to automatically find and report on spindle and zona metrics, including mean retardance and orientation offset. NOTE: The Oosight system is compatible with most research-grade microscopes and laser systems. Glass bottom dishes are required.

Model Basic MetaLC Compensator

CPIF

CCD Camera

C-Mount for Camera

Image Capture Software

Automated Spindle FinderTM*

Automated Zona FinderTM*

Reporting Tools

Movie Capture

Oosight Imaging Systems

Your local CRi agent or distributor is: Cambridge Research & Instrumentation, Inc.CRi 35-B Cabot Road Woburn, MA 01801 USA

E-mail: sales@cri-inc.com Website: www.cri-inc.comToll-Free (USA): 1-800-383-7924Direct (Worldwide): +1-781-935-9099

About CRiCambridge Research & Instrumentation, Inc (CRi) is a global leader in biomedical imaging dedicated to providing comprehensive solutions that enable our customers to perform cutting-edge research and provide better healthcare.

CRi technology helps extract physiologically and medically significant information from biological and clinical samples with intact morphological and biochemical context in tissues and organisms. With over 80 patents pending and issued, CRi’s award-winning in-novations are being utilized around the world.

Notice to purchaser: The Oosight™ Imaging System is meant to be used for research purposes only.