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Flow Cytometry OHSU Research Cores and Shared Resources Introduction The Flow Cytometry Shared Resource (FCSR) personnel provide training in data interpretation, experiment design and routine instrument operation, offering investigators the cost-saving option of doing some of the work themselves. Resource personnel also support investigators by providing operator-assisted fluorescence-activated cell sorting, analytical flow cytometry and data analysis. Services 1. Quantitative measurement of fluorescent reporters to assess the distribution of specific molecules within cell populations. 2. Sorting to isolate purified cell populations based on detection of specific probes such as antibodies and fluorescent proteins. 3. Analysis of multiple characteristics such as relative cell size, antibody binding to cell surface or intracellular biomarkers, DNA and RNA content, and fluorescent protein expression. 4. Functional assays to measure apoptosis, enzyme activity or calcium flux. 5. Cells can be sorted into 5-15 mL conical tubes, eppindorf tubes or into multi-well plates. 6. After acquisition of data, investigators will receive data files and/or data plots. In addition, for sorting experiments, investigators will receive sorted cells and an analysis of post-sort purity. Equipment • DVS Sciences CyTOF New! • BD LSRFortessa New! • BD LSR II • BD FACSCanto II • BD FACSVantage cell sorter • BD inFlux cell sorters • MACSQuant • Luminex 200 • BD FACSCalibur Director Philip Streeter Ph.D. Staff Laboratory Manager Miranda Boyd Operator Pamela Canaday Operator Dorian LaTocha Location Marquam Hill Campus Richard Jones Hall Room 5380 3181 SW Sam Jackson Park Road, Portland OR 97239 Email [email protected] [email protected] Phone 503.418.CYTO (2986) Web www.ohsu.edu/flowcytometry The OHSU Flow Cytometry Shared Resource (FCSR) has operated as a core resource for OHSU investigators since 1996 and provides advanced flow cytometry instrumentation, technical expertise and technical services. OHSU's cores are your campus technology partners dedicated to the success of your project. For optimal results, take advantage of the state-of-the-art scientific resources within the OHSU community. www.ohsu.edu/cores

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Page 1: OHSU Research Cores and Shared Resources … Cytometry OHSU Research Cores and Shared Resources ... Quantitative measurement of fluorescent reporters to assess the distribution of

Flow CytometryOHSU Research Cores and Shared Resources

IntroductionThe Flow Cytometry Shared Resource (FCSR) personnel provide training in data interpretation, experiment design and routine instrument operation, offering investigators the cost-saving option of doing some of the work themselves. Resource personnel also support investigators by providing operator-assisted fluorescence-activated cell sorting, analytical flow cytometry and data analysis.

Services1. Quantitative measurement of fluorescent reporters to assess the distribution of specific molecules within cell populations.2. Sorting to isolate purified cell populations based on detection of specific probes such as antibodies and fluorescent proteins.3. Analysis of multiple characteristics such as relative cell size, antibody binding to cell surface or intracellular biomarkers, DNA and RNA content, and fluorescent protein expression.4. Functional assays to measure apoptosis, enzyme activity or calcium flux.5. Cells can be sorted into 5-15 mL conical tubes, eppindorf tubes or into multi-well plates.6. After acquisition of data, investigators will receive data files and/or data plots. In addition, for sorting experiments, investigators will receive sorted cells and an analysis of post-sort purity.

Equipment• DVS Sciences CyTOF New!• BD LSRFortessa New!• BD LSR II• BD FACSCanto II• BD FACSVantage cell sorter• BD inFlux cell sorters• MACSQuant• Luminex 200• BD FACSCalibur

DirectorPhilip Streeter Ph.D.

StaffLaboratory ManagerMiranda Boyd

OperatorPamela Canaday

OperatorDorian LaTocha

LocationMarquam Hill CampusRichard Jones Hall Room 53803181 SW Sam Jackson Park Road,Portland OR 97239

[email protected]@ohsu.edu

Phone503.418.CYTO (2986)

Webwww.ohsu.edu/�owcytometry

The OHSU Flow Cytometry Shared Resource (FCSR) has operated as a core resource for OHSU investigators since 1996 and provides advanced �ow cytometry instrumentation, technical expertise and technical services.

OHSU's cores are your campus technology partners dedicated to the success of your project. For optimal results, take advantage of the state-of-the-art scienti�c resources within the OHSU community. www.ohsu.edu/cores

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OHSU Research Cores and Shared Resources Flow … Research Cores and Shared Resources ... The Flow Cytometry Shared Resource ... Quantitative measurement of fluorescent reporters to

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