MICROPARTICLES OF SNAKEHEAD FISH (CHANNA STRIATUS) FILLET BINDED BY CHITOSAN USING IONIC GELATION METHOD FOR

WOUND HEALING

Dina Rahmawanty*, Effionora Anwar**, Anton Bahtiar**, Silvia Surini**, Raditya Iswandana**

*Department of Pharmacy, Lambung Mangkurat University, Banjarmasin, Indonesia

**Faculty of Pharmacy, University of Indonesia, Jakarta, Indonesia

dinarahmawanty@gmail.com

INTRODUCTION

METHODS

OBJECTIVES

RESULTS

CONCLUSION

PP 05

Presented at The 2nd International Conference on Nutraceutical and Cosmetic Science 2013 (ICNACS 2013)

October 23rd-24th, 2013, at Atlet Century Hotel , Jakarta, Indonesia

Snakehead fish (Channa striatus) has been reported to enhance dermal

wound healing, to reduce post-operative pain (Mat Jais et al, 1994) becouse

having a very good composition of amino acids and fatty acids (Mohsin and

Ambak, 1983; Sabto, 1988). Both amino acids and fatty acids composition

of Snakehead fish involve to enhance wound healing process (Mat Jais et al,

1994). Chitosan has been studied for use in many other biomedical

aplications and has special characteristics and advantages, including

biocompatibility, biodegradability, haemostatic activity, antibacterial

properties, low cost and the ability to accelerate wound healing (Shuan Liu

et al, 2009). Microparticles of Snakehead fish fillet has been prepared by

chitosan ionic cross-linking using ionic gelation method. Chitosan ionic

cross-linking can be performed at room temperature to avoid the use of

organic solvent, uniform microparticles with an adjustable size and has been

widely used in encapsulation of protein and gene drugs (Jie Wang et al,

2011).

Formulas Particles size

Distribution (nm)

Polidispersity

Indeks

Zeta Potential

(mV)

Morphology

(TEM)

1st Formula D(10%) = 491.8

D(50%) = 561.8

D(90%) = 665.5

0.512 (+) 29.15 Spheric-shape

2nd Formula D(10%) = 481.8

D(50%) = 595.8

D(90%) = 828.1

0.456 (+) 29.35 Spheric-shape

40060080010001200140016001800200024002800320036004000

1/cm

10

20

30

40

50

60

70

80

90

100

%T

SmoothSmoothSmooth

sampel kitosan2

Picture 2. Snakehead fish (Channa striatus) fillet powder preparation

The objective of this research was formulate and charactherize

microparticles of Snakehead fish (Channa striatus) fillet binded by

chitosan using ionic gelation method.

REFERENCES

Snakehead fish (Channa striatus) fillet powder

preparation

Microparticles of Snakehead fillet charactherization :

1. Particles size distribution analysis, polydispersity

index analysis, zeta potential analysis using PSA

(Particle Size Analyzer)

2. Morphology observation using TEM (Transmission

Electron Microscope)

3. FT-IR Analysis

Microparticles of Snakehead fillet preparation using ionic

gelation method

Picture 3. FT-IR Spectrum: chitosan (A); sodium tripolyphosphate (B); chitosan-

tripolyphosphate (C)

Picture 4. Morphology observation result

of microparticles using TEM (1st formula)Picture 5. Morphology observation

result of microparticles using TEM

(2nd formula)

Table 3. Charactherization results of microparticles

Microparticles of Snakehead fish (Channa striatus) fillet was formulated with chitosan

ionic cross-linking using ionic gelation method. Particle size distribution of microparticles

1st formula are 491.8 – 665.5 nm, polidispersity index 0.512, zeta potential (+) 29.15 mV

and spherical-shape morphology; particle size distribution of microparticles 2nd formula

are 481.8 – 828.1 nm, polidispersity index 0.456, zeta potential (+) 29,35 mV, and

spherical-shape morphology.

A

BC

1. Jie Wang , Jun., Zhao Wu Zeng., Ren Zhong Xiao., Tian Xie., Guang Lin Zhou., Xiao Ri Zhan., Shu Ling Wang., (2011).

Recent Advance of Chitosan Nanoparticles as Drug Carriers. Review International Journal of Nanomedicine. 6 765-774.

2. Mat Jais A. M., Mc Culloch R. and Croft K. D. (1994). Fatty Acid and Amino Acid Composition of Haruan- Potential

Role in Wound Healing. Gen Pharmacol.

3. Mohsin A. K. and Ambak M. A. (1983) Freshwater Fishes of Peninsular Malaysia, pp. 157-161. Universiti Pertanian

Malaysia (Publ.), Serdang, Malaysia.

4. Sabto A. (1988) Environmental Physiological Studies of Haruan in Sabah an Honor Thesis Submitted to Biology

Department, Universiti Kebangsaan Malaysia, Sabah.

5. Shuan Liu-Bai, Tsung-Bin Huang, Chun-Hsu Yao, Shr-Shin Fang, Chen- Jung Chang. (2009). Novel Wound Dressing of

Non-woven Fabric Coated with Genipin-crosslinked Chitosan and Bella striata Herbal Extract. Journal of Medical and

Biological Engineering, 29(2): 60-67.