Nutritional Requirements of Tissue culture

1. Salts. 2. Carbohydrate . 3. Amino acids. 4. Vitamins.

Basic Principles of Tissue culture

1. Environment ( Temperature,hydrogen ion concentration , essential metabolites , matrix ).2. Prevention of contamination, Removal of harmfulMetabolites.

Applications:1. Embryoloy ( IVF, Stem cells & Cloning).2. Virology ( Vaccines).3. Genetic ( Human genome , karyotype of

diseases, etc.

Tissue culture

Kinds of techniques:

1. Culture in clot of cover slip.

2. Culture on a watch glass .

3. Culture on petri dishes.

4. Culture on ordinary test tubes , Erlenmeyer flask , round bottomed flasks.

Steps of IVF In Vitro Fertilization (IVF) provides an

alternative method of achieving a pregnancy for couples who have tried and have been unsuccessful with other fertility treatment.

steps are as follows: 1. Retrieval of the mature egg. 2. Sperm collection, fertilization, and embryo

development.  3. Transfer of the developing embryo(s) into the

uterus. 4. Implantation and growth of the embryo(s). 

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Information for all prospective IVF patients :

• All female partners entering the program be tested for antibodies against Rubella. 

• Alcohol, and cigarette smoking should be eliminated during the IVF cycle. 

• Sitting in hot tubs and saunas is not recommended during the IVF cycle.

• Do not begin any new exercise, sport, or intense athletic training during the IVF cycle.

• All women attempting conception should be taking a specific prenatal vitamin.  No other supplements should be taken unless specifically prescribed.

IVF

Application of tissue Culture

Application of tissue Culture