nucleus and mitosis vibs 443/602. structure: pancreatic acinar cells identification: nucleus,...
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Nucleus and Mitosis
VIBS 443/602
Structure: pancreatic acinar cells
Identification: nucleus, nucleolus, heterochromatin
1. Heterochromatin
2. Nucleus
3. Nucleolus
Pancreas158
Pancreas158
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Nuclei with nucleoli
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Structure: electron micrograph of hepatocyte (EM 5)
Identification: nuclear envelope, inner and outer membrane, nuclear pores
1. Nuclear envelope
2. Heterochromatin
3. Euchromatin
4. Nuclear pore
EM 2b
nucleuscytoplasm
Structure: electron micrograph
of stomach (EM 15)
Identification: honeycomb nucleolus called nucleonema
1. Nucleus
2. Nucleonema
Structure: freeze etched of small lymphocyte (EM 6)
Identification: fracture plane at one of the nuclear pores jumped from the outer to the inner membrane of the nuclear envelope
1. Inner nuclear membrane
2. Outer nuclear membrane
3. Nuclear pores
Mitosis anaphase102
Mitosis - metaphase102
Mitosis - anaphase102
ORIENTATION
SEGREGATIONALIGNMENT
Three major events in mitosis
CHROMOSOME MOVEMENT
1
2
3
Three contributions Astral microtubules
Structure: intestinal absorptive cells
Identification: nucleus, nucleolus
1. Nuclei
2. Nucleoli
201
152
Intestine crypt mitotic activity.
1. Brush border
2. Interphase nucleus
3. Anaphase
4. Artifact
201
duodenum152
duodenum152
duodenum152
duodenum152
duodenum152
Ileum148
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Cells in mitosis
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EM 10f: Arteriolar wall; 30,000x.
1. Apical caveolae
2. Collagen
3. Dense bodies
4. Elastic fiber
5. Endothelial cell
6. Nuclear pore
7. RER
EM 19c: Sertoli cell; 13,300x.
1. Golgi
2. Heterophagic vacuole
3. Lipid droplet
4. Microtubule
5. Nuclear pores (cross-section)
6. Sertoli cell
7. Synaptoneural complex
Colchicine treatment – chromosomes do not separate and NE forms around each chromosome due to breakdown of MT.
1. Multinucleated cell
Cytochalasin B treatment-breaks down microfilaments-nucleus activity but cells do not undergo cytokinesis
1. Binucleated cell
2. Nucleolus
Cytochalasin B treatment – chromosomes separate into two normal size nuclei, but the cytoplasm does not split in two
EVENTS IN MITOSIS
CYTOKINESIS
Nucleus and Mitosis