nss 2016 ppt
TRANSCRIPT
![Page 1: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/1.jpg)
Induced breeding of Tinfoil Barb (Barbonymus schwanenfeldii) (Bleeker, 1854)
using Ovaprim™.
By
E.D.M. Epasinghe, A.M.A.N. Adikari, T.A.D.W. Karunaratne,
H.M.P. Kithsiri, V. Pahalawattarachchi
![Page 2: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/2.jpg)
Tinfoil Barb [TFB]§ Globally popular aquarium Cyprinid § Native to Southeast Asia§ No authentic records on its natural
spawning in captive condition in Sri Lanka
Objective of breeding TFB
§ Inducing the spawning of captive reared TFB using Ovaprim
§ Introducing less time consuming and fruitful breeding method for commercial scale fish breeders in Sri Lanka
![Page 3: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/3.jpg)
Materials and methods
Inducing agent - Ovaprim ™
� Uses to induce ovulation and spermiation in fish� A synthetic GnRH [1 mL
contains 20 µg of GnRH + 10 mg Domperidon]
� Ready to inject product –liquid
![Page 4: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/4.jpg)
Selection of brood fish� Selected randomly based on
their external features.
� Distended abdominal region-♀♀� Normal abdominal region- ♂♂
� Sedated fish in 65 mg/L TMS
� Females were subjected to Intra Ovarian Biopsy.
� Males were checked for secretion of milt
Materials and methods
![Page 5: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/5.jpg)
Confirmation of fish maturity,
� 18 females with Migratory stage GV
� 18 males Trickled milt when a slight pressure was applied at the vent
Administration of Ovaprim – within
minimum possible period of time
� Injected single dose
� IM injection to the dorsal musculature
Materials and methods
Avg: BW 0.23 ± 0.01
kg
![Page 6: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/6.jpg)
Germinal vesicle
![Page 7: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/7.jpg)
Dose mL/kg of BW ♀♀
0.20 0.30 0.40 0.50 0.60 Control
Un injected
♂♂ 0.10 0.15 0.20 0.25 0.30 Un injected
• The injected TFBs were kept at 100 L glass tank with preconditioned water – aerating water continuously
• Sex ration 1:1 male to female• Each experimental group comprised of three replicates.
Materials and methodsHormone doses
Breedingperformance
Ovulation time (hrs)
Fertility rate (%)
In addition, datacollected on
Post experimental mortality rate of brooders
Water quality parameters in the experimental tanks
![Page 8: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/8.jpg)
Identification of Ovulation time
� In order to identify the correct ovulating time - after 3hrs by the injection� Females’ vent was slightly pressed very carefully to facilitate
come out eggs- repeated in every half an hour� Did not try by force� Expected free flow of eggs after completion of ovulation
� Collecting of milt� Just after confirmed the complete ovulation in females� Males were stripped first
� Fertilization� Dry method was followed to fertilize eggs
� Fertility rate� Sub sample of eggs checked under the microscope to identify the
first cleavage stage of embryonic development.
![Page 9: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/9.jpg)
Cleavage stages of embryonic development of TFB
4 Cell
16 Cell
Cleavage
Blastula
![Page 10: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/10.jpg)
Results and discussion.
Dose mL/kg of BW ♀♀
0.20 0.30 0.40 0.50 0.60 Control
Un injected
♂♂ 0.10 0.15 0.20 0.25 0.30
Ovulation
time/ hrs
11.00 ±
0.51a
9.20 ±
0.17b
5.43 ±
0.51c
6.10
± 0.17c
3.20 ±
0.17d
not
observed
Fertility
rate %
27.84
± 2.36a
50.5 ±
7.09b
73.33 ±
4.51c
80.67
± 5.13c
33.33 ±
9.07a
not
observed
• Data was analyzed with one way ANOVA and the Tukey test in SPSS software.
• Values are presented as means ± S.D.
• Means in each raw with different superscripts are significantly different from
each other.
![Page 11: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/11.jpg)
11.00
9.20
5.436.10
3.20
0.00
2.00
4.00
6.00
8.00
10.00
12.00
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
Ovu
lati
on ti
me
(hrs
)
Dose- mL/kg of BW
Relationship of Hormone dose Vs Ovulation time
Dosage/mLkg-1
of BW0.20 0.30 0.40 0.50 0.60
P
value
Ovulation
time/ hrs11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d 0.000
Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a 0.000
![Page 12: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/12.jpg)
27.84
50.50
73.33
80.67
33.33
0.00
10.00
20.00
30.00
40.00
50.00
60.00
70.00
80.00
90.00
0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7
Fert
ilit
y ra
te (%
)
Dose- mL/kg of BW
Relationship of Hormone dose Vs Fertility rate
Dosage/mLkg-1
of BW0.20 0.30 0.40 0.50 0.60 P value
Ovulation time/
hrs11.00 ± 0.51a 9.20 ± 0.17b 5.43 ± 0.51c 6.10 ± 0.17c 3.20 ± 0.17d 0.000
Fertility rate % 27.84 ± 2.36a 50.5 ± 7.09b 73.33 ± 4.51c 80.67 ± 5.13c 33.33 ± 9.07a 0.000
![Page 13: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/13.jpg)
Results and discussion.
� In the post spawning period –� survival rate of brooders was 100%� the range of hormone dosages - not harmful
� During the latency period temperature ranged from 27.4 0C – 27.8 0C , pH was measured as 7.3, Breeding tanks were aerated continuosly
![Page 14: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/14.jpg)
Conclusion
� Recommended dosages of obtain highest fertility rates
and relatively short ovulation times are 0.4 -0.5 mlkg-1
single dosage of Ovaprim to the female and half the
dosage to the male at a same time.
• Female fish should be induced when they are at the
peak of GV migratory stage (stage IV)
and,
• Male should ooze sperm with gentle pressure.
![Page 15: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/15.jpg)
Dose calculation� This is a 1.0 mL cyringe.� It has been graduated in to the 50 units.� Therefore, a one unit is equal to 0.02 mL� We need to adjust 1 unit = 0.005 mL of hormone
Normal saline 0.75 ml 0.25 ml hormone
Dose mL/kg of BW ♀♀
0.2 0.3 0.4 0.5Recommended doseby producer
0.6 Control
Un injected
♂♂ 0.1 0.15 0.2 0.25 0.3 Un injected
For 0.23 kg For 0.23 kg of BW /unitof BW /unit
9 14 18 23 284.5 7 9 12 14
![Page 16: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/16.jpg)
To improve hatchability� Introduce different hatching techniques
in order to increase the hatching rate � Eggs are semi buoyant
Future research needs
Effect of other hormone products which contains GnRH on TFB breeding should be monitored
• WOVA.FH™, Ovatide®, Ovulin® - synthetic products
![Page 17: NSS 2016 ppt](https://reader034.vdocuments.us/reader034/viewer/2022042515/58cfe40f1a28ab11578b4ac5/html5/thumbnails/17.jpg)