nsa08 roberts scallop
DESCRIPTION
TRANSCRIPT
Bay scallop genetic diversity and population structure in
Massachusetts
Steven RobertsSchool of Aquatic and Fishery Sciences
University of Washington
Outline
•Beginning
•Marker Development
•Populations / Enhancement
•Conclusions
• ESTs = 7057
• CoreNucleotides = 85
• Contigs 845
• Singletons 2746
• Unique Seqs 3591
Individual clones sequencedD-hinge 671Met 61Set 932Adductor 367Gonad 58
8% sequences <200 bpAverage length - 719 bp970 singletons217 contigs
NCBI Present StatusLibrary Characteristics
Enhancement
Population Differences
Self-assignment
Only Adults Random = 11%Chatham = 60%
Assessing Enhancement
private alleles showing up post enhancement
Summary
• Identify markers that effectively identify all potential parent populations
• functional markers (SSR-ESTs, SNPs)
• Isolation coupled with life history can dramatically affect genetic diversity
• Temporal (yearly) variation is interesting and critical for understanding diversity