north dakota mosquito surveillancethe program was reinstated under the name north dakota mosquito...

2

Collaborators

Margaret Kuklok

Mosquito Surveillance Program Coordinator

Kristi Bullinger

Mosquito Surveillance Report Author

Jenna Smith

Summer Mosquito Analyst

Emily Klusman


Elizebeth Salazar


Kristie Schwarzkopf

Arbovirus Program Supervisor

North Dakota Department of Health

Division of Laboratory Services

2635 East Main Avenue

Bismarck, North Dakota 58506-5520

2017 North Dakota Mosquito

Surveillance Program Mission

Through mosquito collection and speciation, the North Dakota Department of Health (NDDoH) monitors

the risk of infection from arboviral encephalitides that are known to occur in this region. The North

Dakota Mosquito Surveillance team focuses activities on Culex tarsalis, monitoring for increased

numbers in the New Jersey mosquito trap network and viral identification using the CDC miniature light

mosquito trap network. Should mosquito populations reach significant levels or arbovirus activity is

detected, appropriate recommendations for mosquito population control will be issued by the NDDoH to

the vector control districts.

Division of Laboratory Services

3

Table of Contents

North Dakota Mosquito Surveillance Program Background 4

New Jersey Mosquito Trap Network

Operator List 5

2017 Network State Map 6

Network Information 7

2017 Species Comparison Graph 7

2017 Network Mosquito Count Results 8

Arbovirus Information 9

Mosquito Surveillance Risk Assessment Chart 11

Appendix A: 2017 New Jersey Mosquito Trap Data Analysis

Introduction 12

Trap Count Graphs by Region 13

Appendix B: 2007-2017 Weekly New Jersey Mosquito Trap Counts Comparison

Introduction 23

Trap Count Graphs 23

Appendix C: 2017 Weekly Live Trapping Mosquito Counts and PCR Results

Introduction 28

Trap Count Graphs 28

WNV PCR Graphs 50

Appendix D: 2017 Dead Bird Testing

Introduction 51

Dead Bird Graphs 51

References 52

4

North Dakota Mosquito Surveillance Program Background

Since 1975, the North Dakota Department of Health has monitored the mosquito population throughout

the state. The Mosquito Surveillance Program traditionally has been activated following arboviral

outbreaks or flooding incidents in various locations statewide. The program was first initiated in 1975 following an outbreak of western equine encephalitis (WEE) and

St. Louis encephalitis (SLE) in the United States. In 1977, the program was officially formed under the

title, North Dakota Arboviral Encephalitis Surveillance Program and housed with the Division of

Environmental Sanitation and Food Protection. This program was responsible for equine and human

arbovirus surveillance until 1989.

The program was reinstated under the name North Dakota Mosquito Surveillance Program in 1994 in

response to flooding of the Red River in 1993. This program was operated by the Division of

Microbiology until 1997.

In 2000, the North Dakota Mosquito Surveillance Program was reinstated in response to the 1999 West

Nile virus (WNV) outbreak in New York. In 2002, North Dakota had its first confirmed human cases of

WNV, as well as detectable virus through laboratory testing in birds, horses and mosquitoes.

The 2003, program was expanded from 50 New Jersey mosquito traps to a network of 87 traps and 18

CDC miniature light mosquito traps. These enhancements provided network coverage statewide. The

2004 program further expanded the trap network to include 94 New Jersey mosquito traps and 33 CDC

miniature light mosquito traps. A video also was produced to aid in trap placement training.

The 2005, program was further expanded to 103 New Jersey mosquito traps and 39 CDC miniature light

mosquito traps. The program for 2006 had 100 New Jersey traps in operation, with at least one in each

county. The dry conditions during the 2006 season kept the mosquito numbers low when compared to

other seasons, and it was decided to postpone any live trapping.

In 2007, due to increased Culex tarsalis numbers in the state, 97 New Jersey traps were used. In July, live

trapping was initiated in Grand Forks, and on the grounds of the laboratory in Bismarck. Four out of 17

pools collected at the laboratory tested positive for West Nile virus. All 14 pools collected by Grand

Forks and the five pools collected by Bismarck were negative. In 2008, trappers across the state

maintained a New Jersey light trap network of 92 traps. Live trapping was not re-implemented in 2008 by

the Division of Laboratory Services - Microbiology.

In 2009, 91 total New Jersey light traps were in operation. Despite the spread of West Nile virus and

proof it is established in our state, funding for many programs is limited and live trapping was

discontinued in 2010. The New Jersey light trap portion of the program has not been affected. The

program was run essentially unchanged from 2010 to 2015, with varying amounts of New Jersey light

traps.

In 2016, live trapping was re-instated by the Division of Laboratory Services-Microbiology on a limited

basis to assess for the feasibility and utility of re-implementing the program. Five sites utilized the CDC

Minature Light Trap. The mosquitos collected by the live traps were tested by Taqman® Real Time RT-

PCR for West Nile Virus (WNV), LaCrosse Encephalitis (LCE), Western Equine Encephalitis (WEE), St.

Louis Encephalitis (SLE), and Zika virus (ZIKA). Of the 91 pools tested, 15 tested positive for WNV,

with no pools testing positive for LCE, WEE, SLE, or Zika. In 2017, the live trapping was expanded to

include nine sites, collecting two traps each, as well as testing for Eastern Equine Encephalitis (EEE), and

Jamestown Canyon Virus (JCV). Over the 14-week season, 322 pools were tested with 29 pools testing

positive for WNV. No pools tested positive for LCE, WEE, EEE, JCV, SLE, or Zika.

5

New Jersey Mosquito Trap Network

The New Jersey mosquito trap network monitors mosquito populations throughout the state. By identifying

mosquito populations known to be competent encephalitis vectors, officials can determine the threat of mosquito-

borne encephalitis in various regions. Thank you to the following New Jersey mosquito trap and live trap operators whose dedication and commitment to

the North Dakota Department of Health Mosquito Surveillance Program made the 2017 program a success! *Indicates State Park ** Indicates National Wildlife Refuge ∆ Indicates Live Trappers

Location Trapper Location Trapper Location Trapper Arrowwood** Paulette Scherr/

Tyrone Acencio/ Josh Houghtaling

Finley Brittany Ness Maddock Pam Lee & Keith Maddock

Beaver Lake* James Loken Fort Abraham Lincoln*

Dan Schelske McVille Ryan Johnson

Bismarck∆ ND DoH Mosquito Surveillance Program Staff

Fort Ransom* Tyler Modlin & Terry Anderson

Minot∆ Lisa Otto

Bottineau Keith Fulsebakke Fort Stevenson* Chad Troutman New Hradec Kevin Pavlish

Bowman Erin Gaugler & Bill Whitworth

Grahams Island* Henry Duray & Ryan Nelson

New Rockford George Ritzke

Cando Monte Miller Grand Forks∆ Todd Hanson and Grand Forks Vector Control Staff

Oakes Robert Schaefer

Carrington Lisa Solwey & Kristin Larson

Hazelton Bev Voller Pembina Ken Norby

Carson Donna VandenBurg Hettinger Julie Kramlich Rolla Scott Hanson

Cooperstown Nancy Paintner & Julie Ferry

Hillsboro Jim Anderson Thompson Jeremy Aasen

Crosby Dennis Lampert Icelandic* Matt Korhnak Towner Jeffrey Smette

Cross Ranch* Char Binstock/ Eric Lang

Jamestown∆ City of Jamestown Vector Control

Turtle River* Shane Grove

Deep Water Creek Bay

Kerry Hartman Lake Metigoshe* Bill Demming Valley City Jeff Differdin

Devils Lake∆ Myron Aselson Lake Tschida Michelle Psyck Wahpeton∆ Josh Meyer

Dickinson∆ Denny Smith Langdon Rob Gilseth Williston∆ Francis Bosch and Williston Vector Control Staff

Enderlin Rick Gillund Lewis & Clark* Ryan Gardner

Fargo∆ Ben Prather and Fargo Vector Control Staff

Lisbon Randy Seelig

6

2017 New Jersey Mosquito Trap Surveillance Sites & Regions

The following counties are included in each region:

Region Counties

I Williams, Divide, McKenzie and Mountrail

II Ward, Bottineau, Burke, McHenry, McLean, Renville, Sheridan

III Ramsey, Benson, Cavalier, Eddy, Pierce, Rolette, Towner

IV Grand Forks, Griggs, Nelson, Pembina, Walsh

V Cass, Ransom, Richland, Sargent, Steele, Traill

VI Stutsman, Barnes, Dickey, Foster, LaMoure, Logan, McIntosh, Wells

VII Burleigh, Emmons, Grant, Kidder, Mercer, Morton, Oliver, Grant

VIII Stark, Adams, Billings, Bowman, Dunn, Golden Valley, Hettinger,

Slope

7

New Jersey Mosquito Trap Network Information

In 2017, the New Jersey mosquito trap network had a total of 39 active traps across North Dakota. There

were eleven traps in state parks and one in a national wildlife refuge.

At the beginning of the mosquito trapping season, usually Memorial Day, the New Jersey mosquito trap

operator installs a trap in a suitable location. Using a programmable timer, the trap is set to operate from

dusk to dawn seven nights a week. At the end of the seven-day period, the trap contents are collected and

sent to the North Dakota Department of Health, Division of Laboratory Services – Microbiology (DLSM)

in Bismarck for counting and speciation. This process is repeated weekly until Labor Day.

At the DLSM, mosquito surveillance personnel sort the mosquitoes by sex and genera. Since male

mosquitoes do not bite, they are of little health concern. However, their numbers are monitored because

male mosquitoes hatch first, and increased numbers may indicate an increase in future female mosquito

population. The female mosquitoes are separated into four genera: Anopheles, Aedes, Culex and Culiseta.

These genera are then enumerated.

▪ Anopheles is associated with malaria and West Nile virus.

▪ Aedes is associated with illnesses such as canine heartworm, LaCrosse encephalitis (LCE), eastern

equine encephalitis (EEE), western equine encephalitis (WEE), California encephalitis (CAE), and

West Nile virus (WNV). Although Aedes vexans has been shown to be capable of laboratory

transmission of WNV, its mammalian feeding preferences decrease its potential as an enzootic

vector for WNV.

▪ Culex is the mosquito of greatest public health concern in North Dakota, since all species are

competent vectors of Saint Louis Encephalitis (SLE), WEE and WNV. The species most

commonly associated with encephalitis in North Dakota is Culex tarsalis, a principal arbovirus

vector in rural agricultural ecosystems.

▪ Culisetas are monitored due to its association with eastern equine encephalitis.

8

Anopheles Aedes

Aedes

vexans Culex

Culex

tarsalis Culiseta Other

Total

female

T o tal

mo squito e

s

% Trap

sites

submitted

May 29- June 4 167 9 73 55 120 32 0 0 327 494 63.93%

June 5- June 11 187 27 215 25 58 0 3 0 410 597 88.52%

June 12- June18 193 20 231 145 97 70 17 0 628 821 90.16%

June 19- June 25 371 10 271 125 131 176 29 0 790 1,161 90.16%

June 26- July 2 667 38 1,410 196 213 568 6 0 2,470 3,137 88.52%

July 3- July 9 1,585 72 4,339 620 170 323 3 0 8,191 9,776 85.25%

July 10- July 16 556 0 1,549 132 118 135 5 84 3,099 3,655 83.61%

July 17- July 23 1,562 36 2,334 21 107 630 1 93 3,614 5,176 83.61%

July 24- July 30 803 16 324 68 94 326 22 29 989 1,792 88.52%

July 31- Aug 6 548 14 285 59 53 289 14 3 965 1,513 90.16%

Aug 7- Aug 13 131 1 305 50 22 96 22 1 526 657 88.52%

Aug 14- Aug 20 145 20 189 54 33 101 5 0 421 566 80.32%

Aug 21- Aug 27 134 0 97 39 96 39 13 7 376 510 77.05%

Aug 28- Sept 3 329 0 827 333 125 47 35 6 1,752 2,081 68.85%

2017 Totals 7,378 263 12,449 1,922 1,437 2,832 175 223 24,558 31,936

2017 Trap

Averages Total

Week of Male

Female

2017 New Jersey Mosquito Trap Count Totals by Week - Counties

Week of Male Anopheles AedesAedes

vexansCulex

Culex

tarsalisCuliseta Other

Total

female

T o tal

mo squito e

s

% Trap

sites

submitted

May 29- June 4 27 4 9 5 30 0 0 0 48 75 45.45%

June 5- June 11 306 23 309 42 23 3 1 0 401 707 63.63%

June 12- June18 422 18 383 159 67 24 4 0 655 1,077 72.72%

June 19- June 25 468 64 366 307 79 41 3 0 860 1,328 90.90%

June 26- July 2 356 16 378 63 302 81 6 0 846 1,202 63.63%

July 3- July 9 354 41 2682 93 424 83 10 644 3977 4,331 90.90%

July 10- July 16 293 22 916 74 240 25 0 322 1,599 1,892 90.90%

July 17- July 23 309 44 674 14 121 11 8 209 1,081 1,390 72.72%

July 24- July 30 65 6 74 3 13 30 8 43 177 242 72.72%

July 31- Aug 6 82 6 143 22 9 15 8 8 211 293 81.81%

Aug 7- Aug 13 93 7 162 21 10 15 6 0 221 314 72.72%

Aug 14- Aug 20 55 0 36 29 5 7 1 0 78 133 81.81%

Aug 21- Aug 27 272 0 277 217 25 1 34 8 562 820 81.81%

Aug 28- Sept 3 391 19 429 70 26 2 11 0 557 934 54.54%

2017 Totals 3,493 270 6,838 1,119 1,374 338 100 1,234 11,273 14,738

2017 Trap

Averages Total

Female

2017 New Jersey Mosquito Trap Count Totals by Week - State Parks

9

Arbovirus Information

More than 2,500 different species of mosquitoes are found worldwide, with about 200 species in the

United States and at least 34 of these in North Dakota. The most common vector in the spread of

arboviruses is the mosquito; however, not all mosquitoes are vectors in the transmission of arboviruses.

Male mosquitoes feed almost exclusively on nectar and therefore, do not bite. Female mosquitoes lay

eggs that require a blood meal, so female mosquitoes bite warm- or cold-blooded animals and birds.

Stimuli that influence biting include a combination of carbon dioxide, temperature, moisture, smell, color

and movement. Humans are seldom the first or second choice for a blood meal. Horses, cattle, smaller

mammals and birds are preferred. Although acquiring a blood meal is essential for female egg

production, both male and female mosquitoes are mainly nectar feeders.

Mosquito-borne diseases cause more than one million human deaths every year. Some of these diseases

include protozoan infections such as malaria; filarial pathogens such as canine heartworm; and viruses

that cause dengue, yellow fever and encephalitis.

Arthropod-borne viruses (arboviruses) are the most diverse and serious diseases transmitted to susceptible

vertebrate hosts by mosquitoes. Humans and domestic animals can develop clinical illness but usually are

“dead end” hosts because they do not typically contribute to the transmission cycle. Notable exceptions

include Zika virus, Chikungunya virus, and Dengue virus.

West Nile virus (WNV) is named after the West Nile region of Uganda, where it was first discovered in

1937. The Culex species of mosquitoes are the primary vectors, particularly Culex tarsalis in North

Dakota. Common in many parts of the world, WNV had not been seen in the United States until late

summer 1999, when it made its debut in New York. WNV traveled westward across the continent the

following year, with the first human case in North Dakota being reported in 2002. West Nile fever can be

characterized by fever, headache and rash, but can also present with more serious symptoms. Up to 80

Week of Male Anopheles AedesAedes

vexansCulex

Culex

tarsalisCuliseta Other

Total

female

T o tal

mo squito e

s

% Trap

sites

submitted

May 29- June 4 0 0 1 1 1 1 0 0 4 4 100.00%

June 5- June 11 0 0 0 0 0 0 0 0 0 0 100.00%

June 12- June18 0 0 2 0 0 0 0 0 2 2 100.00%

June 19- June 25 0 0 0 0 0 0 0 0 0 0 100.00%

June 26- July 2 0 0 0 0 0 0 0 0 0 0 0.00%

July 3- July 9 0 0 1 0 0 2 0 0 3 3 100.00%

July 10- July 16 0 0 2 0 1 0 0 0 3 3 100.00%

July 17- July 23 0 1 0 0 0 3 0 0 4 4 100.00%

July 24- July 30 0 0 0 0 0 0 0 1 1 1 100.00%

July 31- Aug 6 0 0 0 0 0 0 0 0 0 0 100.00%

Aug 7- Aug 13 0 0 0 0 0 0 0 0 0 0 100.00%

Aug 14- Aug 20 0 0 0 0 0 0 0 0 0 0 100.00%

Aug 21- Aug 27 10 0 0 0 2 2 0 0 4 14 100.00%

Aug 28- Sept 3 0 0 2 0 0 0 0 0 2 2 100.00%

2017 Totals 10 1 8 1 4 8 0 1 23 33

2017 Trap

Averages Total

Female

2017 New Jersey Mosquito Trap Count Totals by Week - National Wildlife Refuges

10

percent of people are asymptomatic with a WNV infection. And although only a small percentage of

people infected with WNV display symptoms, WNV can cause encephalitis (an inflammation of the

brain) and meningitis (inflammation of the brain and spinal cord) in humans and animals, and even death

in some cases. According the CDC website, WNV was responsible for 94 deaths in 2016 in the United

States, two of which occurred in North Dakota. Additional information about West Nile virus in North

Dakota is available at www.ndhealth.gov/wnv.

Western equine encephalitis (WEE) is mostly found in states west of the Mississippi River, Wisconsin,

and Illinois. The primary vector is Culex tarsalis. Birds are the most important host. Since 1964, there

have been fewer than 1,000 cases reported. Human cases have historically been reported in North Dakota;

however, the last documented case in our state was in 1991. Human mortality rates are about 5 percent

and horse mortality rates are considerably higher.

Eastern equine encephalitis (EEE) is spread to horses and humans by infected mosquitoes. Annually,

there are a small number of cases nationwide, although no cases have historically been reported in North

Dakota. Most cases of EEE are seen in the Atlantic and gulf coast states. EEE is the most serious of the

arboviruses that can affect the central nervous system (CNS), resulting in severe complications and even

death. Symptoms may range from none at all, to flu-like, to more serious infections with sudden fever and

severe headache, followed by seizures and coma. About one third of infected patients die, and of those

who survive, many suffer permanent CNS damage. The primary vector is Culiseta melanura, which

feeds only on birds. There are other vectors that spread the virus to other mammals, including various

Culex and Aedes species.

St. Louis encephalitis (SLE) is transmitted from birds to mammals by an infected mosquito. SLE was

discovered in 1933 in St. Louis, Mo. Since then, SLE has been reported in 46 states. Most infections of

SLE do not result in illness, with mild cases exhibiting aseptic meningitis, or fever. The elderly and very

young children are more susceptible, with fatality rates from 2 to 20 percent, and neurologic dysfunction

occurring in about 1 percent of survivors. Nineteen human cases were reported in North Dakota from

1964 to 2010.

The California serogroup is a group of related viruses that include California encephalitis, La Crosse

encephalitis, and Jamestown Canyon virus. Each year, about 75 cases are reported in the United States,

with the majority of illnesses resulting from La Crosse encephalitis. The California serogroup viruses

primarily affect children younger than 16 and more often infect males, than females. Infections are mild,

with a mortality rate of about four deaths per 1,000 infections. Severe cases of La Crosse virus can affect

the nervous system resulting in seizures, partial paralysis, and mental issues. The La Cross virus is seen

in a mosquito-small rodent-mosquito cycle, with spillover into the human population, with humans being

the “dead end” host. Examples of small rodents include chipmunks and squirrels. The virus is generally

seen in mosquitos from heavily wooded areas. The female Aedes triseriatus, can pass the virus to the

eggs she lays. This virus can remain dormant through the winter and develop into infected mosquitos in

the spring. The California serogroup viruses are not considered a major agricultural or public health

threat in North Dakota.

Zika virus is the arbovirus of most recent concern. For the first time, outbreaks in the Western

Hemisphere were reported in 2015. Local transmission was seen in Central and South America, the

Caribbean, and Mexico. In 2016, the first case of local transmission was reported in the United States.

The primary vectors of the Zika virus are Aedes aegypti and Aedes albopictus, neither of which is known

to be present in the Midwest. Zika remains as a concern as it is known to have human to human

transmission. Local outbreaks may occur as a result of the virus being transported by infected travelers.

11

Symptoms of Zika virus include: fever, rash, joint pain, conjunctivitis, and may include muscle pain and

headache. An infection with Zika virus during pregnancy has been linked to the birth defect, such as

microcephaly, defects of the eye, hearing deficits, as well as growth impairments. There also appears to

be a connection between the Zika virus and increased incidents of Guillain-Barré syndrome. Additional

information about Zika virus can be found on the CDC website.

North Dakota Mosquito Surveillance

Risk Assessment Chart

for Arbovirus Activity

Risk

Category

Probability of

Human

Outbreak

Definition of

Conditions

Recommended Response

by Mosquito Surveillance Team

and North Dakota Vector

Control Personnel

1a Remote Mid-season; first week of July; no

observed epizootic activity; low

population counts of vector species

from New Jersey trap network

Begin preliminary, low-intensity

CDC live-trapping network and

testing in all areas of the state; test

for targeted virus presence

1b Late-season; third week of July

through September; no observed

epizootic activity; high population

counts from New Jersey trap

network

Deploy mid-intensity CDC live-

trapping network and viral testing

in areas with high population

counts of targeted vector species;

continue low-intensity trapping

and testing in other areas

2 Low Sporadic epizootic activity in birds

or mosquitoes

Deploy high-intensity CDC live-

trapping network and viral testing

in epizootic areas, and consider

preliminary control measures such

as source reduction and larval

control; continue surveillance in

other areas

3 Moderate Initial confirmation of virus in horse

or human; moderate activity in birds

or mosquitoes

Continue as in Category 2;

consider adult mosquito control as

indicated by surveillance activity

4 High Measures suggesting high risk of

human infection (for example, high

dead bird densities, high mosquito

infection rates, multiple positive

mosquito species, horse or mammal

cases indicating escalating epizootic

transmission, or a human case)

Response as in Category 3;

initiate adult mosquito control

program in areas of potential

human risk

5 Outbreak in

progress

Multiple confirmed human cases;

conditions as listed in Category 4

Implement emergency adult

mosquito control program; if

widespread, consider aerial

spraying

12

Appendix A

New Jersey Mosquito Trap Data Analysis

The mosquito’s life cycle has four separate and distinct stages: egg, larva, pupa and adult. A female

mosquito breeds in the presence of water and lays fertile eggs after obtaining a blood meal. The location

in which a female mosquito deposits her eggs in the environment depends upon larval habitat preference.

The 34 mosquito species indigenous to North Dakota can be grouped into four that reflect their larval

habitat preference; permanent pool, the transient water, the floodwater, and the artificial container and

tree-hole.

Mosquitoes within the permanent pool group, Anopheles and Culex species, lay eggs either singly or

side by side on the water surface of permanent ponds and lakes. Permanent pool mosquitoes can develop

continuously in warm water and hatch daily into adults. Transient water mosquitoes, such as Culex

tarsalis, prefer to lay their eggs in pools of a temporary nature. Common habitats of the transient water

group are roadside ditches, canals, ground pools and irrigated lands. Transient water mosquito eggs found

in ditches and small depressions must wait until rainfall to begin the hatching process. Floodwater

mosquitoes, the Aedes species, lay eggs singly on damp soil or along vegetated shorelines; the eggs

remain dormant until these areas are flooded. Once flooded, the eggs hatch if conditions are favorable.

Large numbers of larvae emerge, and adults can appear as early as six days after flooding. A major

rainstorm, a series of showers, or irrigation sufficient enough to produce standing water promotes

hatching in the floodwater species of mosquitoes. The artificial container and tree-hole group of

mosquitoes place their eggs inside the wall of a container or depression inside a tree, at or above the

water line, and the eggs hatch when the water levels rise. A heavy rain resulting in standing water in areas

such as old tires, tin cans and flowerpots, will begin the hatching process for artificial container

mosquitoes.

Once hatched, larvae of all species emerge and live in water. After four stages, or instars, the larva molts

into a pupa. The pupa stage is a resting, non-feeding stage, where the pupa is encased until the adult

matures and emerges from the skin after one-and-a-half to four days. Adult male mosquitoes hatch first

and live from six to seven days. Female mosquitoes can live for about two weeks, but have been found to

survive for up to five months with ample food. Peak adult mosquito populations usually appear within

two weeks after a number of eggs hatch.

Along with increased rainfall, warmer water temperatures speed up hatching and larval development. If

outdoor temperatures are 50 degrees or higher, productive breeding sites readily produce mosquito larvae.

With increasing water temperatures, large mosquito populations can emerge within one week. Research in

laboratory settings has shown that if the water temperature exceeds 100 degrees, it takes only three to four

days for larval metamorphosis; if the temperature is 90 degrees, it takes five days; and a lower water

temperature of 70 degrees decreases rate of growth to 10 days. Floodwater species of Aedes larvae

generally metamorphose within five to seven days after hatching. The species Culex tarsalis completes its

life cycle in 14 days at 70 degrees and in only 10 days at 80 degrees. On the other hand, some species

have naturally adapted to go through their entire life cycle in as little as four days or as long as one month.

When a mosquito becomes an adult, the weather elements affect its peak activity. Most mosquitoes are

active from dusk until dawn when wind speeds are less than eight miles per hour, the air temperature is

between 65 and 80 degrees, and the weather is moderate.

13

Heavy rains, gusting winds, and cool or high daytime temperatures all limit a mosquito's feeding activity.

At temperatures lower than 50 degrees, mosquitoes become sluggish, reducing their host-seeking

behavior. At higher temperatures, usually during daytime hours, adult mosquitoes seek cover in vegetated

or humid areas with shade.

Region I



14

Region II



15

Region III



16

Region IV


New Jersey Mosquito Trap Analysis

17

Region V



18

Region VI



0

25

50

75

100

125

150

175

200

225

1 2 3 4 5 6 7 8 9 10 11 12 13 14

Ave

rag

e W

ee

kly

Co

un

t

Week

Region VI Average Weekly Counts of Female Culex tarsalis: 2008-2017

2008

2009

2010

2011

2012

2013

2014

2015

2016

2017

19

Region VII



20

Region VIII



21

State Parks



22

National Wildlife Refuges



23

Appendix B 2007-2017

Weekly New Jersey Mosquito Trap Counts Comparison

Appendix B includes graphs of the annual trap counts from the last week of May through the first

week of September. These graphs depict how the mosquito trap counts have changed between

2007 and 2017. Each year, the general trend of North Dakota’s mosquito population is a steady

rise in population that peaks in early to late July, followed by a gradual decrease through the rest of

the mosquito season. Yearly and weekly variances in trap numbers can be attributed to factors such

as rainfall, temperature and wind speed.

28

Appendix C: 2017

Weekly Live Trapping Mosquito Counts and PCR Results

Appendix C includes the weekly enumeration of the Culex spp., Aedes spp., and

Anopheles/Culiseta spp. From the last week of May through the first week of September.

Also included are the PCR results of the seven viruses tested: West Nile Virus, La Cross

Encephalitis, Western Equine Encephalitis, Eastern Equine Encephalitis, Jamestown

Canyon Virus, St. Louis Encephalitis, and Zika Virus.

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #1 0 0 Not Tested 97 3 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 0 1329 53 0 0

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #2 0 0 Not Tested 98 2 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 0 738 14 0 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 Not Submitted

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 Not Submitted

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1Not Submitted

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2Not Submitted

Total Mosquitos

in Trap

Total Counts

Live Trapping Mosquito Counts 2017

Week: 1 Dates: 05.29.207-06.04.2017

Culex spp. Aedes spp. Anopheles/Culisetta spp.

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

29

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#20 0 Not Tested 0 0 Not Tested 0 0 Not Tested

Total Mosquitos

in Trap0 0 0 0 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap0 0 0 0 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#20 2 Negative 15 0 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 2 15 0 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#10 0 Not Tested 8 6 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 0 8 6 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap0 0 2 0 0 0

*Targets Tested: West Nile Virus, Western Equine Encephalitis, Eastern Equine Encephalitis,

LaCrosse Virus, Jamestown Canyon Virus, Saint Louis Encephalitis, and Zika Virus


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #10 0 Not Tested 1 21 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 0 1 21 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #20 1 Negative 2 2 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 1 2 2 0 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1 Not Submitted

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #2 Not Submitted

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#10 0 Not Tested 15 7 Negative 0 1 Negative

Total Mosquitos

in Trap0 0 15 7 0 1

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#20 0 Not Tested 23 6 Negative 0 1 Negative

Total Mosquitos

in Trap0 0 23 6 0 1

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap0 0 1 1 0 0

Total Counts

Total Counts

Total Counts

Aedes spp. Anopheles/Culisetta spp.Culex spp.

Total Counts

Total Counts

Total Counts

Total Counts

30

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #1 2 0 Negative 56 44 Negative 2 0 Negative

Total Mosquitos

in Trap2 0 1301 770 2 0

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #2 0 3 Negative 90 10 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 3 1901 109 0 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 0 0 Not Tested 12 0 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 0 12 0 0 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 0 0 Not Tested 1 0 Negative 3 0 Negative

Total Mosquitos

in Trap0 0 1 0 3 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1Not Submitted

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2Not Submitted

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap1 2 5 6 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap1 0 2 8 0 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1 0 0 Not Tested 9 0 Negative 0 0 Not Tested

Total Mosquitos

in Trap0 0 9 0 0 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap0 0 10 1 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#13 1 Negative 31 2 Negative 1 0 Negative

Total Mosquitos

in Trap3 1 31 2 1 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap0 1 7 6 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap0 0 0 0 0 0

Total Counts


Week: 2 Dates: 6.5.17-6.11.17


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


31

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap0 0 0 1 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap0 1 40 2 0 1

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap0 0 1 0 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap0 15 1 0 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#20 3 Negative 0 0 Not Tested 0 1 Negative

Total Mosquitos

in Trap0 3 0 0 0 1

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap33 31 502 1861 0 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap68 62 848 1502 0 7

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 2 2 Negative 0 5 Not Tested 1 0 Not Tested

Total Mosquitos

in Trap2 2 0 5 1 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 3 0 Negative 17 6 Negative 0 0 Not Tested

Total Mosquitos

in Trap3 0 17 6 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Total Counts


Week: 3 Dates: 06.12.2017-06.18.2017


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

32

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #123 0 Negative 3 1 Not Tested 0 0 Not Tested

Total Mosquitos

in Trap23 0 3 1 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #224 7 Negative 5 4 Not Tested 1 0 Not Tested

Total Mosquitos

in Trap24 7 5 4 1 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1 5 5 Negative 3 12 Negative 0 0 Not Tested

Total Mosquitos

in Trap5 5 3 12 0 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap0 4 3 9 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap0 0 0 0 0 0

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap0 0 11 1 0 2

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#1PWT

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#2PWT

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#10 2 Not Tested Negative 0 10 Negative

Total Mosquitos

in Trap0 2 0 10

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap2 2 96 53 2 23

Anopheles/Culisetta spp.


Culex spp.

Total Counts

Total Counts

Total Counts

Total Counts

Aedes spp.

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

~20 Total

~20 Total

Total Counts

Total Counts

Total Counts

33

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #1 14 7 Neg 42 58 Neg 2 0 NT

Total Mosquitos

in Trap14 7 831 1451 2 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap0 16 965 771 4 5

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 0 2 NT 0 5 NT 0 0 NT

Total Mosquitos

in Trap0 2 0 5 0 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 0 2 NT 1 0 NT 19 0 Neg

Total Mosquitos

in Trap0 2 1 0 19 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #11 10 Neg 47 53 Neg 1 1 NT

Total Mosquitos

in Trap1 10 54 62 1 1

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #20 9 NT 52 48 Neg 3 0 NT

Total Mosquitos

in Trap0 9 52 54 3 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1 2 0 Neg 12 6 Neg 0 0 NT

Total Mosquitos

in Trap2 0 12 6 0 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #2 3 1 Neg 39 25 Neg 0 0 NT

Total Mosquitos

in Trap3 1 39 25 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#19 7 Neg 73 27 Neg 0 0 NT

Total Mosquitos

in Trap9 7 150 110 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#20 0 NT 7 8 Neg 0 0 NT

Total Mosquitos

in Trap0 0 7 8 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#10 1 NT 0 0 NT 0 0 NT

Total Mosquitos

in Trap0 1 0 0 0 0


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Week: 4 Dates: June 19-June 25


34

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#20 0 NT 0 0 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 0 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#1 PWT

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#2 PWT

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#11 5 Neg 40 33 Neg 0 0 NT

Total Mosquitos

in Trap1 5 40 33 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#20 0 NT 2 0 NT 0 0 NT

Total Mosquitos

in Trap0 0 2 0 0 0


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap64 8 627 1381 1 2

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap9 2 677 1776 1 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 1 3 Neg 6 7 Neg 5 0 NT

Total Mosquitos

in Trap1 3 6 7 5 0

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 1 2 Neg 1 9 Neg 22 0 Neg

Total Mosquitos

in Trap1 2 1 9 22 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Total Counts


Week: 5 Dates: 06.26.2017-07.02.2017


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

35

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #17 22 Neg 46 54 Neg 0 0 NT

Total Mosquitos

in Trap7 22 152 162 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #23 6 Neg 39 51 Neg 0 0 NT

Total Mosquitos

in Trap3 6 39 51 0 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#11 1 Neg 1 1 NT 0 0 NT

Total Mosquitos

in Trap1 1 1 1 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#20 0 NT 38 62 Neg 0 0 NT

Total Mosquitos

in Trap0 0 74 97 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#1

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#20 0 NT 0 1 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 1 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#10 0 NT 0 0 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 0 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#20 3 NT 18 82 Neg 0 1 NT

Total Mosquitos

in Trap0 3 33 253 0 1

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#13 1 Neg 37 34 Neg 0 0 NT

Total Mosquitos

in Trap3 1 37 34 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#23 0 Neg 44 56 Neg 2 4 NT

Total Mosquitos

in Trap3 0 64 108 2 4

Total Counts

Total Counts

Total Counts

Aedes spp. Anopheles/Culisetta spp.


Culex spp.

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

36

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#132 2 Negative 6 71 Negative 0 0 NT

Total Mosquitos

in Trap32 2 6 71 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap15 18 439 1848 2 4

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#13 0 Negative 0 0 NT 0 0 NT

Total Mosquitos

in Trap3 0 0 0 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap27 9 24 80 1 2

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1 9 0 Negative 0 0 NT 0 0 NT

Total Mosquitos

in Trap9 0 0 0 0 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap29 0 0 0 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #15 2 Negative 23 54 Negative 0 0 NT

Total Mosquitos

in Trap5 2 23 54 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap6 2 82 211 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#2

Total Mosquitos

in Trap


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Week: 6 Dates: 07.03.2017-07.09.2017


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

37

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 9 26 Negative 26 74 Negative 13 6

176 Coquillettidia

perturbans

Total Mosquitos

in Trap9 26 745 4553 13 6

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 7 5 Negative 24 76 Negative 24 1

120 Coquillettidia

perturbans

Total Mosquitos

in Trap23 5 1144 1929 24 1

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap35 74 86 2094 17 17

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap2 8 198 702 1 0

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #1 92 8 Negative 23 77 Negative 0 0 NT

Total Mosquitos

in Trap92 8 2284 5559 0 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap830 166 931 3969 0 0


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#198 2

Positive

West Nile 8 46 Negative 1 0 NT

Total Mosquitos

in Trap326 3 8 46 1 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap34 55 528 444 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap7 1 5 4 0 0 1

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#20 0 NT 0 3 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 3 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap


Week: 7 Dates: 07.10.2017-07.16.2017


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

38

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1 30 0 Negative 18 80 Negative 0 0 NT

Total Mosquitos

in Trap30 0 18 80 0 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap19 0 6 5 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap10 5 6 23 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #20 3 NT 6 33 Negative 0 0 NT

Total Mosquitos

in Trap0 3 6 33 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap1 2 7 5 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


78

Total Mosquitos

in Trap4 1 7 13 0 0

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 8 22 Negative 25 75 Negative 5 7 Negative ~192

Total Mosquitos

in Trap8 30 353 1131 5 7

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 5 17 Negative 12 88 Negative 18 4 Negative

Total Mosquitos

in Trap5 17 320 3648 18 4 224

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#10 1 Not Tested 9 91 Negative 2 0 NT

Total Mosquitos

in Trap0 1 12 124 2 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap4 3 24 223 1 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap870 286 1103 4117 0 132

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap69 95 393 7963 0 25Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts



Culex spp.

39

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap537 11 58 290 0 2

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap142 6 64 708 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap44 0 0 1 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap4 0 1 1 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake


Total Mosquitos

in Trap43 9 31 88 0 9

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake


Total Mosquitos

in Trap256 28 35 113 1 19

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap7 4 59 504 0 3

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap8 3 44 132 0 1

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap752 32 96 1920 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap128 16 32 288 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap2 1 1 4 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap6 0 0 5 0


Week: 8 Dates: 07.17.17-07.23.17


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


40

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 5 0 Negative 33 67 Negative 76 1 Negative

Total Mosquitos

in Trap5 0 305 1203 124 1

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 3 2 Negative 17 83 Negative 5 3 NT

Total Mosquitos

in Trap3 2 113 659 5 3

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap31 10 31 176 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap1 6 20 96 1 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap1183 5 203 2073 0 11

# of Mosquitos

Tested for PCR

Williams

(Region I)Williston #2 94 6

Positive

West Nile 4 96

Positive

West Nile 0 51 Negative

Total Mosquitos

in Trap1886 6 36 448 0 83Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#1100 0

Positive

WNV4 16 Negative 1 0 NT

Total Mosquitos

in Trap138 0 4 16 1 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap51 0 1 3 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake


Total Mosquitos

in Trap165 5 0 56 3 7

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake


Total Mosquitos

in Trap135 5 18 138 0 11

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Week: 9 Dates: 7/24-30


41

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap28 1 18 246 0 2

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap99 0 5 26 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #199 1

Positive

WNV0 14 Negative 0 0 NT

Total Mosquitos

in Trap191 1 0 14 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap88 1 10 57 0 2

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap1 0 1 7 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap3 0 0 0 0 0

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 19 3 Negative 6 94 Negative 6 3 NT

Total Mosquitos

in Trap19 3 30 370 2 3

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap25 6 59 165 132 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap8 2 5 20 1 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap6 0 5 70 0 1

# of Mosquitos

Tested for PCR

Williams


Positive

WNV3 97 Negative 10 3 Negative

Total Mosquitos

in Trap781 55 99 1153 10 3

# of Mosquitos

Tested for PCR

Williams


Positive

WNV1 92

Postive

WNV0 22

Positive

WNV

Total Mosquitos

in Trap1506 98 1 124 0 54



Culex spp.

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

42

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap42 2 175 481 0 1

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap1 0 140 236 4 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap15 3 1 1 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap4 1 0 0 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap2 3 1 7 0 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap36 21 5 5 2 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #10 4 NT 2 8 Negative 0 0 NT

Total Mosquitos

in Trap0 4 2 8 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #21 1

Positive for

West Nile0 8 NT 0 1 NT

Total Mosquitos

in Trap1 1 0 8 0 1

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#10 0 NT 0 0 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 0 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#21 0 0 1 NT 0 0 NT

Total Mosquitos

in Trap1 0 0 1 0 0


Week: 10 Dates: 7/31-8/6


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Total Counts

43

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap50 15 366 756 13 1

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 63 14

Positive for

West Nile21 79

Positive for

West Nile50 1

Positive for

West Nileother 16

Total Mosquitos

in Trap68 14 216 447 50 1

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap8 2 27 46 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap8 2 69 132 0 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap78 12 37 815 1 3

# of Mosquitos

Tested for PCR

Williams


Positive for

West Nile3 35

Positive for

West Nile0 6 NT

Total Mosquitos

in Trap474 162 3 43 0 14


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#168 1

Positive West

Nile Virus24 76 Negative 0 0 NT

Total Mosquitos

in Trap68 1 152 316 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap1 0 36 207 2 2

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson

#115 0

Positive West


Total Mosquitos

in Trap15 0 7 5 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap3 0 0 0 0 1

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake


Total Mosquitos

in Trap114 0 0 25 0 2

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2100 0

Positive West

Nile Virus3 48

Positive West

Nile Virus1 0 NT

Total Mosquitos

in Trap252 0 3 48 1 0

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Week: 11 Dates: August 7-13


44

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap2 1 0 1 0 0

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #2 9 1 Negative 0 0 NT 1 0 NT other 2

Total Mosquitos

in Trap9 1 0 0 1 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #12 0 Negative 0 1 NT 0 0 NT

Total Mosquitos

in Trap2 0 0 1 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap3 1 4 3 0 0

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#2

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

ResultsComments

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap20 6 41 117 7 2

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap9 0 23 132 77 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#10 1 NT 2 5 NT 0 0 NT

Total Mosquitos

in Trap0 1 2 5 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap1 0 16 20 0 0

# of Mosquitos

Tested for PCR

Williams


Positive West


Total Mosquitos

in Trap90 10 26 250 1 8

# of Mosquitos

Tested for PCR

Williams


Positive West


Total Mosquitos

in Trap226 114 4 20 0 0



Culex spp.

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

45

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#11 0

P ositive for

We st Nile

Virus0 1 NT 0 0 NT

Total Mosquitos

in Trap1 0 0 1 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#20 0 NT 0 2 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 2 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap5 0 7 7 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap16 1 0 1 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1100 0 Negative 16 3 Negative 6 18

Total Mosquitos

in Trap111 0 16 3 6 18

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#298 0

P ositive for

We st Nile

Virus2 0 NT 2 3

Total Mosquitos

in Trap98 0 2 0 2 3

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap20 2 1 2 1 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap18 0 2 1 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand


Total Mosquitos

in Trap22 1 1 3 1 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#2

Total Mosquitos

in Trap


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


Week: 12 Dates: Aug 14-20


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

46

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap1 1 4 5 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#20 1 NT 12 47 Negative 0 0 NT

Total Mosquitos

in Trap0 1 12 47 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap6 0 7 11 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap16 4 13 19 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Total Counts

Total Counts

Total Counts

Total Counts


Week: 13 Dates: Aug 21-27


Total Counts

Total Counts

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot #1 27 7

P ositive for

We st Nile

Virus34 85

P ositive for

We st Nile

Virus9 1 Negative

Total Mosquitos

in Trap27 7 34 85 9 1

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap10 4 61 118 21 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#10 1 NT 1 1 NT 0 0 NT

Total Mosquitos

in Trap0 1 1 1 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap3 0 2 4 0 0

# of Mosquitos

Tested for PCR

Williams


P ositive for

We st Nile

Virus27 73 Negative 0 9 NT

Total Mosquitos

in Trap157 29 48 139 0 9

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap729 156 5 34 0 8


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

47

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap3 3 0 0 0 0

# of Mosquitos

Tested for PCR

Cass


Total Mosquitos

in Trap3 1 0 1 0 0

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap5 4 1 2 0 1

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown


Total Mosquitos

in Trap1 0 0 0 0 0

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap6 3 188 314 3 4

# of Mosquitos

Tested for PCR

Ward (Region

II)Minot#2 3 4 Negative 15 19 Negative 2 1 NT

Total Mosquitos

in Trap3 4 15 19 2 1

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap2 2 0 0 0 0

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap8 1 17 8 0 0

# of Mosquitos

Tested for PCR

Williams


Total Mosquitos

in Trap18 11 98 569 0 0

# of Mosquitos

Tested for PCR

Williams


P ositive for

We st Nile

Virus8 49 Negative 0 9 NT

Total Mosquitos

in Trap131 39 8 49 0 9



Culex spp.

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

48

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck

#10 0 NT 0 0 NT 0 0 NT

Total Mosquitos

in Trap0 0 0 0 0 0

# of Mosquitos

Tested for PCR

Burleigh

(Region VII)

Bismarck


Total Mosquitos

in Trap0 1 43 60 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap0 0 80 150 0 0

# of Mosquitos

Tested for PCR

Stark (Region

VIII)

Dickinson


Total Mosquitos

in Trap0 0 12 11 0 0

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Ramsey

(Region III)

Devils Lake

#2

Total Mosquitos

in Trap

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Cass

(Region V)Fargo #2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Grand Forks

(Region IV)

Grand

Forks #2

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#1

Total Mosquitos

in Trap

# of Mosquitos

Tested for PCR

Stutsman

(Region VI)

Jamestown

#2

Total Mosquitos

in Trap


Week: 14 Dates: 08.28.17-09.04.17


Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


49

Culex

tarsalisCulex spp.

PCR

Results

Aedes

vexansAedes spp.

PCR

Results

Anopheles

spp.

Culisetta

spp.

PCR

Results

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap9 6 114 218 0 5

# of Mosquitos

Tested for PCR

Ward (Region


Total Mosquitos

in Trap5 0 14 37 3 7

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton


Total Mosquitos

in Trap0 0 11 30 0 1

# of Mosquitos

Tested for PCR

Richland

(Region V)

Wahpeton

#20 0 NT

Total Mosquitos

in Trap0 0

# of Mosquitos

Tested for PCR

Williams


P ositive for

We st Nile

Virus17 83

P ositive for

We st Nile

Virus0 12 Negative

Total Mosquitos

in Trap20 11 47 248 0 12

# of Mosquitos

Tested for PCR

Williams


P ositive for

We st Nile

Virus8 85 Negative 0 21 Negative

Total Mosquitos

in Trap57 33 8 85 0 21

*Targets Tested: West Nile Virus, Western Equine Encephalitis, Eastern Equine Encephalitis,

LaCrosse Virus, Jamestown Canyon Virus, Saint Louis Encephalitis, and Zika Virus

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts

Total Counts


50


Taqman® Real Time RT-PCR results for West Nile Virus

51

Appendix D: 2017

Dead Bird Testing

In 2017, the North Dakota Department of Health mosquito surveillance team began testing

dead birds from around the state for mosquito-borne illness. As birds are the primary host

for mosquitos to feed upon, mosquito-borne infections may show up in the bird population

before the human population. To perform this testing, RNASoundTM RNA Sampling Cards

were used. RNASoundTM RNA Sampling Cards were sent to vector control sites around

the state, where the vector control personnel swabbed the oral cavity, or throat, of the bird.

The swab was then rolled over the card, which contains a solution that stabilizes the virus.

The card was then submitted for testing. The cards were tested in the same manner as the

live mosquito pools, and tested for the same seven viruses: West Nile Virus, Western

Equine Encephalitis, Eastern Equine Encephalitis, Jamestown Canyon Virus, La Cross

Encephalitis, St. Louis Encephalitis, and Zika Virus.

Submission

WeekCity Date of Collection Date of Submission ID Number PCR Results Bird Identification

1 Bismarck 5/30/2017 5/30/2017 BDBS17013 Neg Albino Robin

Williston ? 6/8/2017 WDBS17009 Neg Unknown

Williston 6/9/2017 6/16/2017 WDBS17001 Neg Redwing Blackbird

3 Grand Forks 6/12/2017 6/16/2017 GFDBS17001 Neg Grackle

Grand Forks 6/21/2017 7/3/2017 GFDBS17012 Positive WNV Crow


Bismarck 06/27/2017 6/27/2017 BDBS17014 Neg Robin

Williston ? June ? WDBS17003 Neg Unknown

Williston ? June ? WDBS17012 Neg Unknown




Grand Forks 6/29/2017 7/3/2017 GFDBS17010 Negative Grackle

Jamestown 7/7/2017 7/12/2017 JDBS17013 Negative Crow

Fargo 7/11/2017 7/13/2017 FDBS17001 Positive WNV Crow

Fargo 7/12/2017 7/14/2017 FDBS17002 Negative Crow

Grand Forks 7/13/2017 7/26/2017 GFDB17008 Negative Robin

Fargo 7/18/2017 7/21/2017 FDBS17012 Negative Robin

Grand Forks 7/18/2017 7/24/2017 GFDBS17007 Negative Pigeon

Elgin 7/19/2017 7/21/2017 EDBS17001 Negative Robin



Grand Forks 7/20/2017 7/24/2017 GFDBS17006 Negative Juvenielle Robin

Hillsboro 7/28/2017 7/31/2017 HDBS17001 Negative Hawk

Grand Forks 7/27/2017 8/3/2017 GFDBS17003 Negative Duck

Grand Forks 7/28/2017 8/3/2017 GFDBS17004 Negative Duck




Grand Forks 8/1/2017 8/8/2017 GFDBS17013 Negative Crow

Williston 8/4/2017 8/7/2017 WDBS17020 Negative Common Grackle

11 Grand Forks 8/9/2017 8/14/2017 GFDBS17014 Negative Unknown Juvenielle

13 Wahpeton 8/21/2017 8/23/2017 WaDBS17012 Negative Owl

Dead Bird Submissions 2017

4

5

2

8

7

9

10

52

References

1. American Mosquito Control Association. (1990). Organization for Mosquito

Control (16-17). Baltimore, Maryland.

2. Darsie, R.F., Jr. and Ward, R.A. (1981). The Identification and Geographical

Distribution of the Mosquitoes of North America North of Mexico. Fresno,

California: American Mosquito Control Association.

3. Moore, C.G. and Pratt, H.D. (1993). Mosquitoes of Public Health Importance and

Their Control (1-43). Atlanta, GA: Centers for Disease Control and Prevention.

4. Cross, Michael N. (1994, 1995, 1996, 1997, and 1998, September) 1994, 1995,

1996, 1997, and 1998 North Dakota Arbovirus/Mosquito Surveillance Program

Annual Reports. Bismarck, North Dakota: North Dakota Department of Health,

Division of Microbiology.

5. Nies, Nicole. (2000). 2000 North Dakota Mosquito Surveillance Program Report.

Bismarck, North Dakota: North Dakota Department of Health, Division of

Microbiology.

6. Irmen, Chris and Tescher, Todd. (2001, August). 2001 North Dakota Mosquito

Surveillance Program Report. Bismarck, North Dakota: North Dakota Department

of Health, Division of Microbiology.

7. Bold, Tyler and Schulz, Travis. (2002, October). 2002 North Dakota Mosquito

Surveillance Program Report. Bismarck, North Dakota: North Dakota Public

Health Laboratory.

8. Schulz, Travis; Bichler, Mike; Voller, Laura; Hoff, Jennifer. (2003, December).

2003 North Dakota Mosquito Surveillance Program Report. Bismarck, North

Dakota: North Dakota Department of Health, Division of Microbiology.

9. Goddard, Laura, et. al. (December 2002). Vector Competence of California

Mosquitoes for West Nile virus. Emerging Infectious Diseases (Vol. 8, No. 12)

(1385-1391). University of California, Davis, California.

10. Henke, Michelle; Iverson, Sara; Auen, Cari. (2008). 2008 North Dakota Mosquito

Surveillance Program Report. Bismarck, North Dakota: North Dakota Department

of Health, Division of Laboratory Services — Microbiology.

53

11. http://www.ag.ndsu.edu/pubs/plantsci/pests/e472s.htm

12. http://www.cdc.gov/sle/technical/epi.html

13. http://diseasemaps.usgs.gov/sle_nd_human.html

14. http://www.cdc.gov/chikungunya/pdfs/surveillance-and-control-of-aedes-aegypti-

and-aedes-albopictus-us.pdf

15. http://www.terminix.com/pest-control/mosquitoes/facts/mosquito-disease-2015-

2016/

16. RNASoundTM RNA Sampling Card Ready PunchedTM Package Insert. Fortius Bio.

17. The California Department of Public Health Vector-Borne Disease Section. West

Nile Virus Dead Bird Surveillance Program. Safe Practices RNA Preservation

Card Sampling of Dead Birds for West Nile Virus Testing.

18. Foss, Leslie; Reisen, William K;Fang, Ying; Kramer, Vicki; Padgett, Kerry. (2016).

Evaluation of Nucleic Acid Preservation Cards for West Nile Virus Testing in Dead

Birds. PLOS One. June 24, 2016. https://doi.org/10.1371/journal.pone.0157555

http://www.ag.ndsu.edu/pubs/plantsci/pests/e472s.htm

http://www.cdc.gov/sle/technical/epi.html

http://diseasemaps.usgs.gov/sle_nd_human.html

http://www.cdc.gov/chikungunya/pdfs/surveillance-and-control-of-aedes-aegypti-and-aedes-albopictus-us.pdf

http://www.cdc.gov/chikungunya/pdfs/surveillance-and-control-of-aedes-aegypti-and-aedes-albopictus-us.pdf

http://www.terminix.com/pest-control/mosquitoes/facts/mosquito-disease-2015-2016/

http://www.terminix.com/pest-control/mosquitoes/facts/mosquito-disease-2015-2016/

https://doi.org/10.1371/journal.pone.0157555

north dakota mosquito surveillancethe program was reinstated under the name north dakota mosquito...

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