nmr microscopy
DESCRIPTION
NMR Microscopy. Stef VanGorden Joseph Hornak Rochester Institute of Technology. Objective. The main goal of this research was to create a NMR microscope capable of producing a 1 mm thick, approximately 112 micron in-plane resolution tomographic images. Bruker 300 DRX Spectrometer. - PowerPoint PPT PresentationTRANSCRIPT
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NMR Microscopy
Stef VanGordenJoseph Hornak
Rochester Institute of Technology
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Objective
• The main goal of this research was to create a NMR microscope capable of producing a 1 mm thick, approximately 112 micron in-plane resolution tomographic images.
• Bruker 300 DRX Spectrometer
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Overview of my Presentation
• Background
• Experimental
• Applications
• Conclusions
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BACKGROUND
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E = hhB
(Hornak 1997)
Bz/T
Energy Level Diagram
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Gy
Sample with a Uniform Field
(Hornak 1997)
Hydrogen Molecules
S
N
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Sample with Increasing Gradient
Gy
(Hornak 1997) B = x Gx
Hydrogen Molecules
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EXPERIMENTAL
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Starting Point
• Reproduction of previous 1-D and 2-D imaging results.
• Slice Selective Sequence Evaluation
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Slice Selective Sequence
Slice Selective Case (tomographic slice)
Gz
Gy
Gx
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Timing Diagram for the Slice Selective NMR Microscope
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Variables
Thick Slice (From ~1mm to ~10 mm)
Width of 90° pulse = 80 µstw (180°) =160 µs
Amplitude attenuation = 20 dB
Thin Slice(From ~.5 mm to ~1 mm)
Width of 90° pulse = 157 µstw (180°) = 314 µs
Amplitude attenuation = 26 dB
THK = 2/(Gztw)
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Applying Gradients
THK = 2/(Gztw)
100% =50 G/cm Maximum
10% = 5 G/cm => 6 mm
60% = 30 G/cm => 1 mm
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Feature Documentation
In-plane Resolution Phantoms
Slice Thickness Phantoms
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In-plane Resolution
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In-plane Resolution
Fishing line in capillary tubes Glass rods
112 m
284 m
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In-plane Resolution
45 m -- 112 m
object --- image
Object Size vs. Image Size
0
1000
2000
3000
4000
5000
0 2000 4000 6000
Object Size [microns]
Ima
ge
Siz
e
[mic
ron
s] Ideal Values
Actual results
0
100
200
300
400
500
0 50 100 150
1 pixel = 23.7 m
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Slice Thickness Phantom
Screw phantom in sample tube
Signal from the screw phantom.
THK = ( (1/32 in/threads)(25.4 mm/in)THK = .8 mm for an angle of 360°
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Nylon Screw Phantom Images
z
y
x
y
x
y
x
y
x
y
One or more rotations
Less than one rotation
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Cone Phantom Design
Illustration of Cone Phantom
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Cone Phantom Images
Images with slice thickness of 1mm and .5 mm respectively.
.711 mm
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4°
Portion of Sample Tube (profile of middle)
Analyzing Wedge Images
.55mm
4.25mm
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APPLICATIONS
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Celery Images
Day 1: Images Two Weeks Later
Thick slice Thinner slice Thinnest slice
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House Fly Image
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Conclusion
• Moved practical NMR microscopy at RIT closer to reality.
• Current Capabilities slice thickness ~.5 mm in-plane resolution ~ 100
microns
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The Next Step ...
• Slice Selection Locator• SNR improvements• Further In-plane
Resolution Evaluation
x
y
Slice SelectionLocator